Resource data sheet
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pCAhBAFF

Expression vector of human BAFF cDNA

Catalog number RDB03927
Resource name pCAhBAFF
Clone info. human BAFF driven by CA promoter
Vector backbone pCAcc (Plasmid)
Selectable markers Amp^r
Gene/insert name Human TNFSF13B cDNA
Depositor Hamada, Hirofumi |
 
Other clones in our bank human TNFSF13B (NCBI Gene 10673) |

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Terms and conditions set forth by the DEPOSITOR The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit institution for a not-for-profit research. RECIPIENT must contact the DEPOSITOR in the case of application for any patents or commercial use based on the results from the use of the BIOLOGICAL RESOURCE. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested.
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RDB03927 pCAhBAFF DNA solution Please contact us.

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Sequence information

RIKEN BRC has sequenced portions of this material for quality test. Primers and the results are shown below.

Data are summerized on test sheet below.

Test sheet RDB12773_A4Dip1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: pAxCA_F1 RDB12773_A4Dia_2.seq
>03927_12773_A4Di_pAxCAF1_C06_09.ab1
    1 ATGGGGTGCG GCGAGGCACA GGATCTTGCT ACATGTTCAT GCCTTCTTCT TTTTCCTACA
   61 GCTCCTGGGC AACGTGCTGG TTATTGTGCT GTTCCATCAT TTTGGCAAAG AATTCGCCAT
  121 GGATGACTCC ACAGAAAGGG AGCAGTCACG CCTTACTTCT TGCCTTAAGA AAAGAGAAGA
  181 AATGAAACTG AAGGAGTGTG TTTCCATCCT CCCACGGAAG GAAAGCCCCT CTGTCCGATC
  241 CTCCAAAGAC GGAAAGCTGC TGGCTGCAAC CTTGCTGCTG GCACTGCCGT CTTGCTGCCT
  301 CACGGTGGTG TCTTTCTACC AGGTGGCCGC CCTGCAAGGG GACCTGGCCA GCCTCCGGGC
  361 AGAGCTGCAG GGCCACCACG CGGAGAAGCT GCCAGCAGGA GCAGGAGCCC CCAAGGCCGG
  421 CCTGGAGGAA GCTCCAGCTG TCACCGCGGG ACTGAAAATC TTTGAACCAC CAGCTCCAGG
  481 AGAAGGCAAC TCCAGTCAGA ACAGCAGAAA TAAGCGTGCC GTTCAGGGTC CAGAAGAAAC
  541 AGTCACTCAA GACTGCTTGC AACTGATTGC AGACAGTGAA ACACCAACTA TACAAAAAGG
  601 ATCTTACACA TTTGTTCCAT GGCTTCTCAG CTTTAAAAGG GGAAGTGCCC TAGAAGAAAA
  661 AGAGAATAAA ATATTGGTCA AAGAAACTGG TTACTTTTTT ATATATGGTC AGGTTTTATA
  721 TACTGATAAG ACCTACGCCA TGGGACATCT AATTCAGAGG AAGAAGGTCC ATGTCTTTGG
  781 GGATGAATTG AGTCTGGTGA CTTTGTTTCG ATGTATTCAA AATATGCCTG AAACACTACC
  841 CAATAATTCC TGCTATTCAG CTGGCATTGC AAAACTGGAA GAAGGAGATG AACTCCAACT
  901 TGCAATACCA AGAGAAAATG CACAAATATC ACTGGGATGG AGATGTCACA TTTTTTTGGT
  961 GCATTGAAAC TGCTGTGAGG GATCTTTTTT CCCTCTGCCA AAAAATTATG GGGACATCAT
 1021 GAAGCCCCCT TTGAGCATCT GAACTTTCTG GGCCTAATAA AGGAAATTTA TTTTTCAATT
 1081 GCAATAAGTG TGTTGGAATT TTTTTTTGTG TCTCCTCACT CGGTAAGGGA CAATATGGGA
1141 GGCAAATTTC ATTTTAAAAA ACCAATCCAG AAAAATGCAG
//
Primer: pCA-bgter RDB12773_A4Dib_2.seq
>03927_12773_A4Di_pCA_bgter_D06_12.ab1
    1 AGCCCTTGGA TGGTCCCATA ATTTTTGGCA GAGGGAAAAG ATCCTCACAG CAGTTTCAAT
   61 GCACCAAAAA ATGTGACATC TCCATCCAGT GATATTTGTG CATTTTCTCT TGGTATTGCA
  121 AGTTGGAGTT CATCTCCTTC TTCCAGTTTT GCAATGCCAG CTGAATAGCA GGAATTATTG
  181 GGTAGTGTTT CAGGCATATT TTGAATACAT CGAAACAAAG TCACCAGACT CAATTCATCC
  241 CCAAAGACAT GGACCTTCTT CCTCTGAATT AGATGTCCCA TGGCGTAGGT CTTATCAGTA
  301 TATAAAACCT GACCATATAT AAAAAAGTAA CCAGTTTCTT TGACCAATAT TTTATTCTCT
  361 TTTTCTTCTA GGGCACTTCC CCTTTTAAAG CTGAGAAGCC ATGGAACAAA TGTGTAAGAT
  421 CCTTTTTGTA TAGTTGGTGT TTCACTGTCT GCAATCAGTT GCAAGCAGTC TTGAGTGACT
  481 GTTTCTTCTG GACCCTGAAC GGCACGCTTA TTTCTGCTGT TCTGACTGGA GTTGCCTTCT
  541 CCTGGAGCTG GTGGTTCAAA GATTTTCAGT CCCGCGGTGA CAGCTGGAGC TTCCTCCAGG
  601 CCGGCCTTGG GGGCTCCTGC TCCTGCTGGC AGCTTCTCCG CGTGGTGGCC CTGCAGCTCT
  661 GCCCGGAGGC TGGCCAGGTC CCCTTGCAGG GCGGCCACCT GGTAGAAAGA CACCACCGTG
  721 AGGCAGCAAG ACGGCAGTGC CAGCAGCAAG GTTGCAGCCA GCAGCTTTCC GTCTTTGGAG
  781 GATCGGACAG AGGGGCTTTC CTTCCGTGGG AGGATGGAAA CACACTCCTT CAGTTTCATT
  841 TCTTCTCTTT TCTTAAGGCA AGAAGTAAGG CGTGACTGCT CCCTTTCTGT GGAGTCATCC
  901 ATGGCGAATT CTTTGCCAAA ATGATGAGAC AGCACAATAA CCAGCACGTT GCCCAGGAGC
  961 TGTAGGAAAA AGAAGAAAGG CATGAACATG GTTAGCAGAG GCTCTAGAGC CCGCCGGGTC
 1021 ACACGCCAGA AGCCGAAACC CCGCCTGCCC CGTCCCCCCC CGAAGGCAGC CGTCCCCCCC
 1081 GCGGACAGCC CGAGCTGGAG AGGAGAAGGG AACGCGCCGC GACGCACGAG TCCTCCCGAC
1141 CATTTCCTTT CTGTGCCCGG CGGCCGCAAC G
//

Please visit Sequencing and PCR primers for primer information.


References

References


2018.12.10

GNP_filter3_RDBDEP_html_181012.pl