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Please review the QC test results indicated by check icon below as well as clone information before placing your order.

pCAhCD40L

Expression vector of human CD40 ligand.

Catalog number RDB03915
Resource name pCAhCD40L
Clone info. Expression vector of human CD40 ligand driven by CA promoter.
Vector backbone pCAcc (Plasmid)
Selectable markers Amp^r
Gene/insert name Human CD40 ligand cDNA
Depositor|Developer Hamada, Hirofumi |
Other clones in our bank
External Database
Human CD40 ligand
          Reference sequence
            

          Distribution information

          Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
          Terms and conditions set forth by the DEPOSITOR The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit institution for a not-for-profit research. RECIPIENT must contact the DEPOSITOR in the case of application for any patents or commercial use based on the results from the use of the BIOLOGICAL RESOURCE. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested.
          Additional terms and conditions for distribution Regarding resources containing CAG promoter: In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation for the CAG promoter (Niwa, H., Yamamura, K., Miyazaki, J., Gene 108 : 193-200, 1991) and an acknowledgment to Dr. Jun-ich Miyazaki of the Osaka University are requested.
          Ordering Please visit Information of Request for Distribution.[link] 
          Order form           [Credit Card Payment]  [Bank Transfer Payment]
          Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word]
          提供案内 (日本国内) [open/close]

          提供条件 非営利機関の非営利学術研究へ提供する。利用者が本件リソースを利用した研究成果に基づき特許申請、商業活動等を行う場合は、利用者は事前に寄託者に連絡する。利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する。利用者は、研究成果の公表にあたって謝辞の表明を必要とする。
          付加的提供条件 CAGプロモータを含むリソースについて: 利用者は、研究成果の公表にあたってCAGプロモータの文献 (Niwa, H., Yamamura, K., Miyazaki, J., Gene 108 : 193-200, 1991)を引用し、大阪大学 宮崎純一博士への謝辞の表明を必要とする。
          提供依頼 手続きの詳細は、「提供申込みについて[link]」をご覧ください。
          提供依頼書 [Word]
          提供同意書 (MTA、非営利機関による非営利学術研究用)[Word]

          Catalog # Resource name Shipping form Fee (non-profit org.)
          RDB03915 pCAhCD40L DNA solution

          Ordering Information [in Japanese] [in English]
          Please review the QC test results indicated by check icon below as well as clone information before placing your order.

          References and tips

          Electronic file

          Original reference

          original Tomihara, K., Gene transfer of the CD40-ligand to human dendritic cells induces NK-mediated antitumor effects against human carcinoma cells. Int. J. Cancer 120 (7): 1491-1498 (2007). PMID 17205529.

          Further references such as user reports and related articles (go to bottom)

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          Sequence information

          RIKEN BRC has sequenced portions of this material for quality test.
          Please review the QC test results indicated by check icon as well as clone information before placing your order.

          Test sheet RDB12774_A4Dip1.pdf check

          Nucleotide sequence of a portion of this resource (if available).

          Primer: pAxCA_F1
          Sequence file: RDB12774_A4Dia.seq check
          >03915_12774_A4Di_pAxCA_F1_E12_05_ABI08.ab1
              1 CGAACACCCC GGGTTGCTAC ATGTTCATGC CTTCTTCTTT TTCCTACAGC TCCTGGGCAA
             61 CGTGCTGGTT ATTGTGCTGT CTCATCATTT TGGCAAAGAA TTCAGCATGA TCGAAACATA
            121 CAACCAAACT TCTCCCCGAT CTGCGGCCAC TGGACTGCCC ATCAGCATGA AAATTTTTAT
            181 GTATTTACTT ACTGTTTTTC TTATCACCCA GATGATTGGG TCAGCACTTT TTGCTGTGTA
            241 TCTTCATAGA AGGTTGGACA AGATAGAAGA TGAAAGGAAT CTTCATGAAG ATTTTGTATT
            301 CATGAAAACG ATACAGAGAT GCAACACAGG AGAAAGATCC TTATCCTTAC TGAACTGTGA
            361 GGAGATTAAA AGCCAGTTTG AAGGCTTTGT GAAGGATATA ATGTTAAACA AAGAGGAGAC
            421 GAAGAAAGAA AACAGCTTTG AAATGCAAAA AGGTGATCAG AATCCTCAAA TTGCGGCACA
            481 TGTCATAAGT GAGGCCAGCA GTAAAACAAC ATCTGTGTTA CAGTGGGCTG AAAAAGGATA
            541 CTACACCATG AGCAACAACT TGGTAACCCT GGAAAATGGG AAACAGCTGA CCGTTAAAAG
            601 ACAAGGACTC TATTATATCT ATGCCCAAGT CACCTTCTGT TCCAATCGGG AAGCTTCGAG
            661 TCAAGCTCCA TTTATAGCCA GCCTCTGCCT AAAGTCCCCC GGTAGATTCG AGAGAATCTT
            721 ACTCAGAGCT GCAAATACCC ACAGTTCCGC CAAACCTTGC GGGCAACAAT CCATTCACTT
            781 GGGAGGAGTA TTTGAATTGC AACCAGGTGC TTCGGTGTTT GTCAATGTGA CTGATCCAAG
            841 CCAAGTGAGC CATGGCACTG GCTTCACGTC CTTTGGCTTA CTCAAACTCT GAGGATCTTT
            901 TTCCCTCTGC CAAAAATTAT GGGGGACATC ATGAAGCCCC TTTGAGCATC TGACTTTCTG
            961 GGCTATAAAG GAAATTTATT TTTCATTGCA TAGTGTGGTG GAGTTTTTTT TGTGTCTCTC
           1021 CACTTCGGAC GACATATGGA GGCCAATTCC ATTAACATCG GACTGAGAAT TGCTTAGGAG
          1081 TTTGTGGCA
          //

          Please visit Sequencing and PCR primers for primer information.

          References

          Original, user report and related articles

          original Tomihara, K., Gene transfer of the CD40-ligand to human dendritic cells induces NK-mediated antitumor effects against human carcinoma cells. Int. J. Cancer 120 (7): 1491-1498 (2007). PMID 17205529.

          2022.02.09

          GNP_filter3_RDBDEP_html_220112.pl