Resource data sheet
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pCAhCD40L

Expression vector of human CD40 ligand.

Catalog number RDB03915
Resource name pCAhCD40L
Clone info. Expression vector of human CD40 ligand driven by CA promoter.
Vector backbone pCAcc (Plasmid)
Selectable markers Amp^r
Gene/insert name Human CD40 ligand cDNA
Depositor Hamada, Hirofumi |
 
Other clones in our bank human CD40LG (NCBI Gene 959) |

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Terms and conditions set forth by the DEPOSITOR The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit institution for a not-for-profit research. RECIPIENT must contact the DEPOSITOR in the case of application for any patents or commercial use based on the results from the use of the BIOLOGICAL RESOURCE. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested.
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Catalog # Resource name Shipping form Fee (non-profit org.)
RDB03915 pCAhCD40L DNA solution

Ordering Information [in Japanese] [in English]

References and tips

Electronic file

Featured content

References

original Tomihara, K., Gene transfer of the CD40-ligand to human dendritic cells induces NK-mediated antitumor effects against human carcinoma cells. Int. J. Cancer 120 (7): 1491-1498 (2007). PMID 17205529.

User reports and related articles (go to bottom)


Sequence information

RIKEN BRC has sequenced portions of this material for quality test. Primers and the results are shown below.

Data are summerized on test sheet below.

Test sheet RDB12774_A4Dip1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: pAxCA_F1 RDB12774_A4Dia.seq
>03915_12774_A4Di_pAxCA_F1_E12_05_ABI08.ab1
    1 CGAACACCCC GGGTTGCTAC ATGTTCATGC CTTCTTCTTT TTCCTACAGC TCCTGGGCAA
   61 CGTGCTGGTT ATTGTGCTGT CTCATCATTT TGGCAAAGAA TTCAGCATGA TCGAAACATA
  121 CAACCAAACT TCTCCCCGAT CTGCGGCCAC TGGACTGCCC ATCAGCATGA AAATTTTTAT
  181 GTATTTACTT ACTGTTTTTC TTATCACCCA GATGATTGGG TCAGCACTTT TTGCTGTGTA
  241 TCTTCATAGA AGGTTGGACA AGATAGAAGA TGAAAGGAAT CTTCATGAAG ATTTTGTATT
  301 CATGAAAACG ATACAGAGAT GCAACACAGG AGAAAGATCC TTATCCTTAC TGAACTGTGA
  361 GGAGATTAAA AGCCAGTTTG AAGGCTTTGT GAAGGATATA ATGTTAAACA AAGAGGAGAC
  421 GAAGAAAGAA AACAGCTTTG AAATGCAAAA AGGTGATCAG AATCCTCAAA TTGCGGCACA
  481 TGTCATAAGT GAGGCCAGCA GTAAAACAAC ATCTGTGTTA CAGTGGGCTG AAAAAGGATA
  541 CTACACCATG AGCAACAACT TGGTAACCCT GGAAAATGGG AAACAGCTGA CCGTTAAAAG
  601 ACAAGGACTC TATTATATCT ATGCCCAAGT CACCTTCTGT TCCAATCGGG AAGCTTCGAG
  661 TCAAGCTCCA TTTATAGCCA GCCTCTGCCT AAAGTCCCCC GGTAGATTCG AGAGAATCTT
  721 ACTCAGAGCT GCAAATACCC ACAGTTCCGC CAAACCTTGC GGGCAACAAT CCATTCACTT
  781 GGGAGGAGTA TTTGAATTGC AACCAGGTGC TTCGGTGTTT GTCAATGTGA CTGATCCAAG
  841 CCAAGTGAGC CATGGCACTG GCTTCACGTC CTTTGGCTTA CTCAAACTCT GAGGATCTTT
  901 TTCCCTCTGC CAAAAATTAT GGGGGACATC ATGAAGCCCC TTTGAGCATC TGACTTTCTG
  961 GGCTATAAAG GAAATTTATT TTTCATTGCA TAGTGTGGTG GAGTTTTTTT TGTGTCTCTC
 1021 CACTTCGGAC GACATATGGA GGCCAATTCC ATTAACATCG GACTGAGAAT TGCTTAGGAG
1081 TTTGTGGCA
//

Please visit Sequencing and PCR primers for primer information.


References

References

original Tomihara, K., Gene transfer of the CD40-ligand to human dendritic cells induces NK-mediated antitumor effects against human carcinoma cells. Int. J. Cancer 120 (7): 1491-1498 (2007). PMID 17205529.

2018.10.31

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