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pAxCALNLhCCNA1 (forward) (#RDB03557)

Shuttle vector to generate rAd harboring human CCNA1 (forward)

Alternative name 1285C13F
Clone info. Shuttle vector to generate recombinant adenovirus harboring Sugano full-length cDNA clone, CBR04934 (Gene 200: 149-156 (1997)). This clone might have a DNA sequence corresponding 47nt to 1950nt of NM_001111045.1 as 47nt to 431nt and 1751nt to 1950nt of NM_001111045.1 were observed. Known differences (at least): substitutions T to C at 291nt and A to G at 1946nt.
Comment Cre-recombinase is required to remove LNL stuffer sequence.
Vector backbone pAxCALNLw (Cosmid, use packaging extracts for transforming E. coli host)
Size of vector backbone 46074 bp
Selectable markers Amp^r
Gene/insert name Human CCNA1/Cyc A1 cDNA
Depositor|Developer DNA Bank, |
 

Distribution information

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MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ]
Please visit Information of Request for Distribution.[link] For for-profit-research purpose, please contact us. 
Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature (Gene 200: 149-156, 1997) and acknowledgement to the DNA Bank are requested.
Additional terms and conditions:
Regarding resources containing CAG promoter: In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation for the CAG promoter (Niwa, H., Yamamura, K., Miyazaki, J., Gene 108 : 193-200, 1991) and an acknowledgment to Dr. Jun-ich Miyazaki of the Osaka University are requested.
Remarks Remember that you will be working with samples containing infectious virus.
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必要書類
提供依頼書  [依頼書の記入例 ]
提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ]
手続きの概要は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。
MTAに書く使用条件 研究発表に際し、理研DNA Bankヘの謝辞ならびに次の論文の引用を必要とする (Gene 200: 149-156, 1997)。
MTAに書く付加的使用条件：
CAGプロモータを含むリソースについて: 利用者は、研究成果の公表にあたってCAGプロモータの文献 (Niwa, H., Yamamura, K., Miyazaki, J., Gene 108 : 193-200, 1991)を引用し、大阪大学 宮崎純一博士への謝辞の表明を必要とする。
備考 このリソースはウイルス粒子産生用のため、取扱いに注意が必要です。

Catalog # Resource name Shipping form Fee (non-profit org.)
RDB03557 pAxCALNLhCCNA1 (forward) DNA solution JPY 9,460 (not-for-profit academic purpose)
plus cost of shipping containers, dry ice (if required) and shipping charge

Please review the QC test results indicated by check icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The pAxCALNLhCCNA1 (forward) was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB03557).

Reference section:

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB03557_B0Fmp1-1.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: pAxCLNLW_F (Pr0878)
Region: SV40 pA, loxP, insert 5'
Sequence file: RDB03557_B0Fma.seq check
>03557_03557_B0Fm_3_pAxCLNLW_F_A05_01_ABI08.ab1
    1 NNNNNNNNNN NNNNNNNNNN NNNNCTCNNG CGCGGGGNNC TCNTGCTGGN GTTCTTCGCC
   61 CACCCCATCG ATAACTTGTT TATTGCAGCT TATAATGGTT ACAAATAAAG CAATAGCATC
  121 ACAAATTTCA CAAATAAAGC ATTTTTTTCA CTGCATTCTA GTTGTGGTTT GTCCAAACTC
  181 ATCAATGTAT CTTATCATGT CTGGATCAAT CCGAACGCGT ATAACTTCGT ATAGCATACA
  241 TTATACGAAG TTATCTCGAG TCGCTCGGTA CGATTTTCGA GCACTGTTGG CCTACTGGCT
  301 CCCAGGCTGG AATCCCTCGA CACTTGGTCC TTCCCGCCCC GCCCTTCCGT GCCCTGCCCT
  361 TCCCTGCCCT TCCCCGCCCT GCCCCGCCCG GCCCGGCCCG GCCCTGCCCA ACCCTGCCCC
  421 GCCCTGCCCC GCCCAGCCGG CCACCTCTTA ACCGCGATCC TCCAGTGCAC TTGCCAGTTG
  481 TTCCGGACAC ATAGAAAGAT AACGACGGGA AGAGCGGGGC CCGCTTTGGG GTCCAGGCAG
  541 GTCTTGGGGC CTCCTGTCTG GTGGGAGGAG GCCGCAGCGC AGCACCCTGC TCGTCACTTG
  601 GGATGGAGAC CGGCTTTCCC GCAATCATGT ACCCTGGATC TTTTATTGGG GGCTGGGGAG
  661 AAGAGTATCT CAGCTGGGAA GGANCGGGGC TCCCAGATTT CGTCTTCCAG CAGCCCGTGG
  721 AGTCTGAAGC AATGCACTGC AGCAACCCCA AGAGTGGAGT TGTGCTGGCT ACAGTGGCCC
  781 GAGGTCCCGA TGCTTGTCAG ATACTCACCA GANCCCCGCT GGGCCNGGAT NCCCCGCAGA
  841 NGNNAGTGCT ANGGCTGCTN NNNGNNAATG GGCAGTACAG GANGNNCTGN GGCNNGGGGN
  901 NCACNANAAT CANGNGNTNN TCTGGATCAN NAAATGNNNN NCNNCCNNCN NGAAANAAAG
961 CACTNNNNNN NNNN
//
Primer: pCAGGS_R1 (Pr0614)
Region: beta-globin pA, insert 3'
Sequence file: RDB03557_B0Fmb.seq check
>03557_03557_B0Fm_3_pCAGGS_R1_B05_02_ABI08.ab1
    1 NNNNNNNNNN NNNNNNNNNN NNNNNNGNNN TGNNNNNAAT TTCCTTTATT AGCCAGAAGT
   61 CAGATGCTCA AGGGGCTTCA TGATGTCCCC ATAATTTTTG GCAGAGGGAA AAAGATCTCA
  121 GTGGTATTTG TGAGCCAGGG CATTGGCCAC ACCAGCCACC ACCTTCTGAT AGGCAGCCTG
  181 CACCTGAGGA GTGAATTGAT TTTCGAGCAC ATGTGGCCTT TTTTTTTTTT TTTTTTTTTC
  241 ACAAGACAAG CAGTTTATTG TTTAAAAAAT GTTGGACTAT ATTACAAACT ACTAAAGTCT
  301 AAGGGAAACT AAATTCATTT ACATTTTTAA AATGTCATCT ATATTGTAAA CAACATTAGT
  361 TGATCCAACG TGCAGAAGCC TATGACGATT ATTGGCACTT CTGATATGGA GGTGAAGTTC
  421 TGGAAGTGCT TCCATTCANA AACTTATTGT ANAANAANAA CTGCAGGTGG CTCCATGAGG
  481 GACACACACA GGTACTTTGA AGCCTTGTAC TTCTCCCTAA TTGCTTGCTG AGGTCGATGG
  541 GGTATATCAA GGTACGCTTT ATGAAGCTCA CTCAGGCAAG GCACAATTTC ACTTAATGAA
  601 TACCCTGTAA ATGCAGCAAG GGTTTCTGGC CAAAAGTGCT TGTTCACAGT ATAGTTTGCC
  661 AGGCAAAAAG CTGCTGCAGC TATCAGTGAA GGAAGATATT TCAAGANTGG ATCTGCTTCA
  721 AGTAGACTCA GCTCTGCTAC GTACTTAGCC AGGTTCTCAG NCCTGACNCA CACTCCTTGT
  781 CGCCTCAAGT ACTGANNGAN AAANTGGTNG GNGGNTGGTN CTGTCANATC AAAAGCTANA
  841 ANTTTNANAA NCAAGTGTTC CNNTTTTANN AGTTGTCNNT TTGNGTATGT NTCNTNNNNN
  901 ATANNNNNNN AANTCNNNCT ACTTNNNNNA NNNNATNTCT CTNNATNNTT NNGANNNNNN
961 AANNNNNNNN NN
//
Primer: tetpro-B_R (Pr0732)
Region: ITR, Ad5 psi
Sequence file: RDB03557_B0Fmc.seq check
>03557_03557_B0Fm_3_tetpro-B_R_D04_04_ABI08.ab1
    1 NNNNNNNNNN NNNNNNTGNN NNNGNNGGGG TGGNGTTTGT GNNGTGGCGC GGGGCGTGGG
   61 ANCGGGGCGG GTGNNGTAGT AGTGTGGCGG AAGTGTGATG TTGCAAGTGT GGCGGAACAC
  121 ATGTAAGCGA CGGATGTGGC AAAAGTGACG TTTTTGGTGT GCGCCGGTGT ACACAGGAAG
  181 TGACAATTTT CGCGCGGTTT TAGGCGGATG TTGTAGTAAA TTTGGGCGTA ACCGAGTAAG
  241 ATTTGGCCAT TTTCGCGGGA AAACTGAATA AGAGGAAGTG AAATCTGAAT AATTTTGTGT
  301 TACTCATAGC GCGTAATATT TGTCTAGGGC CGCGGGGACT TTGACCGTTT ACGTGGAGAC
  361 TCGCCCAGGT GTTTTTCTCA GGTGTTTTCC GCGTTCCGGG TCAAAGTTGG CGTTTTATTA
  421 TTATAGTCAG CATCGATTTA GCTTGGGCTG CAGGTCGAGG GATCTCCATA AGAGAAGAGG
  481 GACAGCTATG ACTGGGAGTA GTCAGGAGAG GAGGAAAAAT CTGGCTAGTA AAACATGTAA
  541 GGAAAATTTT AGGGATGTTA AAGAAAAAAA TAACACAAAA CAAAATATAA AAAAAATCTA
  601 ACCTCAAGTC AAGGCTTTTC TATGGAATAA GGAATGGACA GCAGGGGGCT GTTTCATATA
  661 CTGATGACCT CTTTATAGCC AACCTTTGTT CATGGCAGCC AGCATATGGG CATATGTTGC
  721 CAAACTCTAA ACCAAATACT CATTCTGATG TTTTAAATGA TTTGCCCTCC CATATGTCCT
  781 TCCNAGTGAG AGACACAAAA AATTCCAACA CACTATTGCA ATGAAAATAA ATTTCCTTTA
  841 TNAGCNNGAA NTCAGANGCT CAANGGGCTT CATGANGTCC CCNTAATTTT TGGCAGANGG
  901 AAAAANATNN CANNGGNNTT TGTGANNCAG GGNNTTNGNC ACACCANNCN NCCNNCNTNN
961 TGANNNNNNN NCNNNNNNNN NN
//
Primer: pAxCA_F1 (Pr0164)
Region: loxP, NeoR
Sequence file: RDB03557_B0Fmd.seq check
>03557_03557_B0Fm_3_pAxCA_F1_C04_03_ABI08.ab1
    1 NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNCCTTCTTC TTTTTCCNNN NNNCTCCTGG
   61 GCAACGTGCT GGTTATTGTG CTGNCTCATC ATTTTGGCAA AGAATTGNNN NNTTCGAGCG
  121 AACGCGTATA ACTTCGTATA GCATACATTA TACGAAGTTA TCTCGAGTCG GATTTGATCT
  181 GATCAAGAGA CAGGATGAGG ATCGTTTCGC ATGATTGAAC AAGATGGATT GCACGCAGGT
  241 TCTCCGGCCG CTTGGGTGGA GAGGCTATTC GGCTATGACT GGGCACAACA GACAATCGGC
  301 TGCTCTGATG CCGCCGTGTT CCGGCTGTCA GCGCAGGGGC GCCCGGTTCT TTTTGTCAAG
  361 ACCGACCTGT CCGGTGCCCT GAATGAACTG CAGGACGAGG CAGCGCGGCT ATCGTGGCTG
  421 GCCACGACGG GCGTTCCTTG CGCAGCTGTG CTCGACGTTG TCACTGAAGC GGGAAGGGAC
  481 TGGCTGCTAT TGGGCGAAGT GCCGGGGCAG GATCTCCTGT CATCTCACCT TGCTCCTGCC
  541 GAGAAAGTAT CCATCATGGC TGATGCAATG CGGCGGCTGC ATACGCTTGA TCCGGCTACC
  601 TGCCCATTCG ACCACCAAGC GAANCATCGC ATCGAGCGAG CACGTACTCG GATGGAAGCC
  661 GGTCTTGTCG ATCAGGATGA TCTGGACGAA GANCATCANG GGCTCGCGCC AGCCGAACTG
  721 TTCGCCAGGC TCNANNGCGC GCATGCCCNA CGGCGANGAT CTCGTCGNGN NCCATGGCGA
  781 TGCCTGCTTG CCNAATATCA TGGTGGAAAA NGGCCGCTTT TCTGGNNTCA TCGACNGNGG
  841 CCGGCTGGGN GTGGCGGANN GCTATCAGGA CATANCGNTN NGCTACCCNN GATATTGNTN
  901 ANNANNNTNG GCGGCNAATG GGNNTGANNN NNTNNCNNCN NGCTTTNNNG NATCGNNGNT
  961 CCNNATTCGC ANCNNNNTCN NNNTTNTANN GNNNTTNNTN NNNNNNNNTN NNTNNNGNNN
1021 NNGNNNNNNN NGGNNNNNNN NAANNNNNNN NNN
//

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles