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pAxCALNLhCCNA1 (forward) (#RDB03534)

Shuttle vector to generate rAd harboring human CCNA1 (forward)

Alternative name 1256C8F
Clone info. Shuttle vector to generate recombinant adenovirus harboring Sugano full-length cDNA clone, CBL05761 (Gene 200: 149-156 (1997)). This clone might have a DNA sequence corresponding 1nt to 1,730nt of NM_003914.4 as 1nt to 410nt and 1399nt to 1730nt of NM_003914.4 were observed. Known difference (at least): A to G sbstitution at 1548nt.
Comment Cre-recombinase is required to remove LNL stuffer sequence.
Vector backbone pAxCALNLw (Cosmid, use packaging extracts for transforming E. coli host)
Size of vector backbone 46074 bp
Selectable markers Amp^r
Gene/insert name Human CCNA1/Cyc A1 cDNA
Depositor|Developer DNA Bank, |
 

Distribution information

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MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ]
Please visit Information of Request for Distribution.[link] For for-profit-research purpose, please contact us. 
Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature (Gene 200: 149-156, 1997) and acknowledgement to the DNA Bank are requested.
Additional terms and conditions:
Regarding resources containing CAG promoter: In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation for the CAG promoter (Niwa, H., Yamamura, K., Miyazaki, J., Gene 108 : 193-200, 1991) and an acknowledgment to Dr. Jun-ich Miyazaki of the Osaka University are requested.
Remarks Remember that you will be working with samples containing infectious virus.
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必要書類
提供依頼書  [依頼書の記入例 ]
提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ]
手続きの概要は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。
MTAに書く使用条件 研究発表に際し、理研DNA Bankヘの謝辞ならびに次の論文の引用を必要とする (Gene 200: 149-156, 1997)。
MTAに書く付加的使用条件：
CAGプロモータを含むリソースについて: 利用者は、研究成果の公表にあたってCAGプロモータの文献 (Niwa, H., Yamamura, K., Miyazaki, J., Gene 108 : 193-200, 1991)を引用し、大阪大学 宮崎純一博士への謝辞の表明を必要とする。
備考 このリソースはウイルス粒子産生用のため、取扱いに注意が必要です。

Catalog # Resource name Shipping form Fee (non-profit org.)
RDB03534 pAxCALNLhCCNA1 (forward) DNA solution JPY 9,460 (not-for-profit academic purpose)
plus cost of shipping containers, dry ice (if required) and shipping charge

Please review the QC test results indicated by check icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The pAxCALNLhCCNA1 (forward) was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB03534).

Reference section:

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB03534_B0Fmp1-1.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: pAxCLNLW_F (Pr0878)
Region: SV40 pA, loxP, insert 5'
Sequence file: RDB03534_B0Fma.seq check
>03534_03534_B0Fm_1_pAxCLNLW_F_A07_01_ABI24.ab1
    1 NNNNNNNNNN NNGCNNNNNN NNCNTCNNGC GCGGGGNNCT CNTGCTGGNN TTCTTCGCCC
   61 ACCCCATCGA TAACTTGTTT ATTGCAGCTT ATAATGGTTA CAAATAAAGC AATAGCATCA
  121 CAAATTTCAC AAATAAAGCA TTTTTTTCAC TGCATTCTAG TTGTGGTTTG TCCAAACTCA
  181 TCAATGTATC TTATCATGTC TGGATCAATC CGAACGCGTA TAACTTCGTA TAGCATACAT
  241 TATACGAAGT TATCTCGAGT CGCTCGGTAC GATTTTCGAG CACTGTTGGC CTACTGGAGT
  301 TGTTCCGGAC ACATAGAAAG ATAACGACGG GAAGAGCGGG GCCCGCTTTG GGGTCCAGGC
  361 AGGTTTTGGG GCCTCCTGTC TGGTGGGAGG AGGCCGCAGC GCAGCACCCT GCTCGTCACT
  421 TGGGATGGAG ACCGGCTTTC CCGCAATCAT GTACCCTGGA TCTTTTATTG GGGGCTGGGG
  481 AGAAGAGTAT CTCAGCTGGG AAGGACCGGG GCTCCCAGAT TTCGTCTTCC AGCAGCAGCC
  541 CGTGGAGTCT GAAGCAATGC ACTGCAGCAA CCCCAAGAGT GGAGTTGTGC TGGCTACAGT
  601 GGCCCGAGGT CCCGATGCTT GTCAGATACT CACCAGAGCC CCGCTGGGCC AGGATCCCCC
  661 GCAGAGGACA GTGCTAGGGC TGCTAACTGC AAATGGGCAG TACAGGANGA NCTGTGGCCA
  721 GGGGATCACA AGAATCANGT GTTATTCTGG ATCAGAAAAT GCCTTCCCTC CNGCTGGAAA
  781 GAAAGCACTC CCTGACTGTG GGGGNNCAAG ANCCCCCCCA AGCAAGGGTT TGACATCTAC
  841 ATGGNTGAAC TANANCNNGG GGGANAGANA CNGCTGCTCN GNNCAGANAN GGGGANGGNN
  901 TTTNANGNNG NGNATGAAGN ANANNANTTT GNCTNNNNNN NNNANNANAC ATACANNAAA
961 NNNNNNNNNN
//
Primer: hCCNA1-M_F (Pr0879)
Region: insert 3'
Sequence file: RDB03534_B0Fmb.seq check
>03534_03534_B0Fm_1_hCCNA1-M_F_D06_04_ABI08.ab1
    1 NNNNNNNNNN NNNNNNNNNC NGCNTTNNCN GNGNANTCNT TAAGTGAAAT TGTGCCTTGC
   61 CTGANTGAGC TTCATAAAGC GTACCTTGAT ATACCCCATC GACCTCAGCA AGCAATTAGG
  121 GAGAAGTACA AGGCTTCAAA GTACCTGTGT GTGTCCCTCA TGGAGCCACC TGCAGTTCTT
  181 CTTCTACAAT AAGTTTCTGA ATGGAAGCAC TTCCGGAACT TCACCTCCAT ATCAGAAGTG
  241 CCAATAATCG TCATAGGCTT CTGCACGTTG GATCAACTAA TGTTGTTTAC AATATAGATG
  301 ACATTTTAAA AATGTAAATG AATTTAGTTT CCCTTAGACT TTAGTAGTTT GTAATATAGT
  361 CCAACATTTT TTAAACAATA AACTGCTTGT CTTATGAAAA AAAAAAAAAA AAAAAAAAAA
  421 AAAAAGGCCA CATGTGCTCG AAAATCAATT CACTCCTCAG GTGCAGGCTG CCTATCANAA
  481 GGTGGTGGCT GGTGTGGCCA ATGCCCTGGC TCACAAATAC CACTGANATC TTTTTCCCTC
  541 TGCCAAAAAT TATGGGGACA TCATGAAGCC CCTTGAGCAT CTGACTTCTG GCTAATAAAG
  601 GAAATTTATT TTCATTGCAA TAGTGTGTTG GAATTTTTTG TGTCTCTCAC TCGGAAGGAC
  661 ATATGGGAGG GCAAATCATT TAAAACATCA NAATGAGTAT TTGGTTNANA GTTTGGCAAC
  721 ATATGCCCAT ATGCTGGCTG CCATGAACAA AGGTTGGCTA TAAAGAGGTC ATCAGTATAT
  781 GAAACAGCCC CCCNGCTGNC CATTCNTNAT TCCATANAAA ANCCTTGACT NGANGNTANA
  841 NTTTTTTTTN ANATTTTGTT TTGNGNNANT TTTTTNNNTN NACATNCCCT AAAAATTTTN
  901 CNTTANNNNT TTTNANNANN CNAGANTTTT TNCNNNCNNN NNCNNNANNN NNNCCNANNN
961 NNNANNNNNN NCNNNNTNNN NNNNNNN
//
Primer: tetpro-B_R (Pr0732)
Region: ITR, Ad5 psi
Sequence file: RDB03534_B0Fmc.seq check
>03534_03534_B0Fm_1_tetpro-B_R_D07_10_ABI24.ab1
    1 NNNNNNNNNN NNNNNNNNNN NNNNAATGNN GGGGTGGNNT TTGTGNNGTG GCGCGGGGCG
   61 TGGGAACGGG GCGGGTGNNG TAGTAGTGTG GCGGAAGTGT GATGTTGCAA GTGTGGCGGA
  121 ACACATGTAA GCGACGGATG TGGCAAAAGT GACGTTTTTG GTGTGCGCCG GTGTACACAG
  181 GAAGTGACAA TTTTCGCGCG GTTTTAGGCG GATGTTGTAG TAAATTTGGG CGTAACCGAG
  241 TAAGATTTGG CCATTTTCGC GGGAAAACTG AATAAGAGGA AGTGAAATCT GAATAATTTT
  301 GTGTTACTCA TAGCGCGTAA TATTTGTCTA GGGCCGCGGG GACTTTGACC GTTTACGTGG
  361 AGACTCGCCC AGGTGTTTTT CTCAGGTGTT TTCCGCGTTC CGGGTCAAAG TTGGCGTTTT
  421 ATTATTATAG TCAGCATCGA TTTAGCTTGG GCTGCAGGTC GAGGGATCTC CATAAGAGAA
  481 GAGGGACAGC TATGACTGGG ANTAGTCAGG AGAGGAGGAA AAATCTGGCT AGTAAAACAT
  541 GTAAGGAAAA TTTTAGGGAT GTTAAAGAAA AAAATAACAC AAAACAAAAT ATAAAAAAAA
  601 TCTAACCTCN ANGTCAAGGC TTTTCTATGG AATAAGGAAT GGACAGCANG GGGCTGTTTC
  661 ATATACTGAT GACCTCTTTA TAGCCAACCT TTGTTCATGG CAGCCAGCAT ATGGGCATAT
  721 GTTGCCNAAC TCTAAACCNA ATACTCATTC TGATGTTTTA AATGANTTGC CCTCCCATAT
  781 GTCCNTCCGA GTGANANACA CAAAAAAAAT TCCNACACAC TATTGCAATG AAAATAANTT
  841 TCCTTTTATT ANNCAGAAAN NCANATGCTC AANGGGCTTC NTGATGTCCC CATANTTTTT
  901 GGNANANGGN AAAAGATNNC ANNGGNNATT NNNGANCCAG GGNATNNGNN NNCNCNNNCN
961 NNCNNCNNTN NNGNNANGNN NNNCNNGNNN NNNNNNN
//
Primer: pCAG-F_V2 (Pr0330)
Region: CAG pro, loxP, NeoR
Sequence file: RDB03534_B0Fmd.seq check
>03534_03534_B0Fm_1_pCAG-F_V2_C06_03_ABI08.ab1
    1 NNNNNNNNNN NNNNNNNNNN GNGCNNNNNN GANTCTGGGN NGCGCCGCCG CACCCCCTCT
   61 AGCGGGCGCG GGGCGNANCG GTGCGGCGCC GGCAGGAAGG AAATGGGCGG GGAGGGCCTT
  121 CGTGCGTCGC CGCGCCGCCG TCCCCTTCTC CCTCTCCAGC CTCGGGGCTG TCCGCGGGGG
  181 GACGGCTGCC TTCGGGGGGG ACGGGGCAGG GCGGGGTTCG GCTTCTGGCG TGTGACCGGC
  241 GGCTCTAGAG CCTCTGCTAA CCATGTTCAT GCCTTCTTCT TTTTCCTACA GCTCCTGGGC
  301 AACGTGCTGG TTATTGTGCT GTCTCATCAT TTTGGCAAAG AATTGATTAA TTCGAGCGAA
  361 CGCGTATAAC TTCGTATAGC ATACATTATA CGAAGTTATC TCGAGTCGGA TTTGATCTGA
  421 TCAAGAGACA GGATGAGGAT CGTTTCGCAT GATTGAACAA GATGGATTGC ACGCAGGTTC
  481 TCCGGCCGCT TGGGTGGAGA GGCTATTCGG CTATGACTGG GCACAACAGA CAATCGGCTG
  541 CTCTGATGCC GCCGTGTTCC GGCTGTCAGC GCAGGGGCGC CCGGTTCTTT TTGTCAAGAC
  601 CGACCTGTCC GGTGCCCTGA ATGAACTGCA GGACGAGGCA GCGCGGCTAT CGTGGCTGGC
  661 CACGACGGGC GTTCCTTGCG CANCTGTGCT CGACGTTGTC ACTGAAGCGG GAAGGGACTG
  721 GCTGCTATTG GGCGAAGTGC CGGGGCANGA TCTCCTGTCA TCTCACCTTG CNCCTGCNGA
  781 GAAAGTATCC ATCATGGCTG ANGCNATGCG GCGGCTGCAT ACGCTTGATN CGGCTANCNG
  841 CCCATTCGAN CACNNAGCNA ANCATCGNAN NCNNNCGNNC NCGTACTNNG ATGNAAANNN
  901 NGNNTNNNNN ATNNNNNANN NTCNGGNNNN AANNNNNTNN NNGGCNNNNN GNNNNNCNNN
961 ANNTNNTNNN NNNNGNNNNN NNNNN
//

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles