Resource data sheet
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Please review the QC test results indicated by check icon below as well as clone information before placing your order.

p3CRE RE-TK hRluc (#RDB03459)

Reporter construct of CRE response element/PMA

Alternative name 2059C2F
Clone info. 3' CRE is cloned into the BglII site of phRL-TK, and the BglII site is killed by the cloning. The nucleotide sequence of the 3' CRE is 5'-GATCCTGACGTCATGACGTCATGACGTCAG-3'.
Comment The nucleotide sequence of the element was determined. Function of the inserted element has not been examined by the depositor.
Vector backbone phRL-TK (Plasmid)
Size of vector backbone 4.0 kb
Selectable markers Amp^r
Gene/insert name Artificial CRE response element/PMA synthesized
Depositor|Developer Yokoyama, Kazunari |
 
Remarks, protocol and/or map (pdf) RDB03459.pdf

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
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Order form [Credit Card Payment] [Bank Transfer Payment] [Example of order form ]
MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ]
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Terms and conditions for distribution The RECIPIENT agrees to expressly describe the acknowledgement of the Gene Engineering Division, RIKEN BRC as the source of the BIOLOGICAL RESOURCE in any publication. The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for-profit organization for a not-for-profit research.
Additional terms and conditions:
The use of the BIOLOGICAL RESOURCE is restricted to the academic researches conducted by non-profit organization. By using this material, the RECIPIENT agrees to be bound by the conditions of the limited use statement of the Promega Corporation.
提供案内 (日本国内) [open/close]

必要書類
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提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ]
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MTAに書く付加的使用条件:
本件リソースの使用は学術機関での学術研究に限る。本件リソースの使用にあたって、利用者はプロメガ社の限定使用条件に従う必要がある。

Catalog # Resource name Shipping form Fee (non-profit org.)
RDB03459 p3CRE RE-TK hRluc DNA solution

Please review the QC test results indicated by check icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The p3CRE RE-TK hRluc was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB03459).

Reference section:

Further references such as user reports and related articles (go to bottom)


QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB03459_A5Jtp1-1.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: Tkpro-R
Sequence file: RDB03459_A5Jta.seq check
1
Primer: pAxCANLF1
Sequence file: RDB03459_A5Jtb.seq check
1

Please visit Sequencing and PCR primers for primer information.


References

Original, user report and related articles

user_report Kimura M., IRF2-binding protein-1 is a JDP2 ubiquitin ligase and an inhibitor of ATF2-dependent transcription. FEBS Lett., 582, 2833-2837 (2008). PMID 18671972. [link to RRC of NBRP]