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Please review the QC test results indicated by check icon below as well as clone information before placing your order.

p3AP1 (PMA) RE-TK hRluc(F)

Reporter construct of AP1 response element/PMA

Catalog number RDB03452
Resource name p3AP1 (PMA) RE-TK hRluc(F)
Alternative name 2055C129F
Clone info. 3' AP1 response element is cloned into the BglII site of phRL-TK, and the BglII site is killed by the cloning. The nucleotide sequence of the 3' AP1 response element is 5'-GATCCTGACTAATGACTAATGACTAAG-3'.
Comment The nucleotide sequence of the element was determined. Function of the inserted element has not been examined by the depositor.
Vector backbone phRL-TK (Plasmid)
Size of vector backbone 4.0 kb
Selectable markers Amp^r
Gene/insert name Artificial AP1 response element/PMA synthesized
Depositor|Developer Yokoyama, Kazunari |
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External Database
Artificial AP1 response element/PMA

            Reference sequence
              

            Distribution information

            Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
            Terms and conditions set forth by the DEPOSITOR The RECIPIENT agrees to expressly describe the acknowledgement of the Gene Engineering Division, RIKEN BRC as the source of the BIOLOGICAL RESOURCE in any publication. The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for-profit organization for a not-for-profit research.
            Additional terms and conditions for distribution The use of the BIOLOGICAL RESOURCE is restricted to the academic researches conducted by non-profit organization. By using this material, the RECIPIENT agrees to be bound by the conditions of the limited use statement of the Promega Corporation.
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            RDB03452 p3AP1 (PMA) RE-TK hRluc(F) DNA solution

            Please review the QC test results indicated by check icon below as well as clone information before placing your order.

            References and tips

            Electronic file

            Remarks, protocol and/or map (pdf) RDB03452.pdf

            Original reference

            Further references such as user reports and related articles (go to bottom)

            Featured content

            Featured content Dnaconda's recommendation EXR0028j (Japanese text)

            Sequence information

            RIKEN BRC has sequenced portions of this material for quality test.
            Please review the QC test results indicated by check icon as well as clone information before placing your order.

            Test sheet RDB13982_A6C7p1.pdf check

            Nucleotide sequence of a portion of this resource (if available).

            Primer: TKproR
            Sequence file: RDB13982_A6C7a.seq check
            >03452_13982_A6C7_TkproR_D05_11.ab1
                1 CCTAGGAAAG TGTTTCGTTC TTCCCCTCCC CCCGCGTCAG ACAAACCCTA ACCACCGCTT
               61 AAGCGGCCCC CGCGAGGTCC GAAGACTCAT TTAGATCCTT AGTCATTAGT CATTAGTCAG
              121 GATCTGTCGA GCCATGTGAG CAAAAGGCCA GCAAAAGGCC AGGAACCGTA AAAAGGCCGC
              181 GTTGCTGGCG TTTTTCCATA GGCTCCGCCC CCCTGACGAG CATCACAAAA ATCGACGCTC
              241 AAGTCAGAGG TGGCGAAACC CGACAGGACT ATAAAGATAC CAGGCGTTTC CCCCTGGAAG
              301 CTCCCTCGTG CGCTCTCCTG TTCCGACCCT GCCGCTTACC GGATACCTGT CCGCCTTTCT
              361 CCCTTCGGGA AGCGTGGCGC TTTCTCATAG CTCACGCTGT AGGTATCTCA GTTCGGTGTA
              421 GGTCGTTCGC TCCAAGCTGG GCTGTGTGCA CGAACCCCCC GTTCAGCCCG ACCGCTGCGC
              481 CTTATCCGGT AACTATCGTC TTGAGTCCAA CCCGGTAAGA CACGACTTAT CGCCACTGGC
              541 AGCAGCCACT GGTAACAGGA TTAGCAGAGC GAGGTATGTA GGCGGTGCTA CAGAGTTCTT
              601 GAAGTGGTGG CCTAACTACG GCTACACTAG AAGAACAGTA TTTGGTATCT GCGCTCTGCT
              661 GAAGCCAGTT ACCTTCGGAA AAAGAGTTGG TAGCTCTTGA TCCGGCAAAC AAACCACCGC
              721 TGGTAGCGGT GGTTTTTTTG TTTGCAAGCA GCAGATTACG CGCAGAAAAA AAGGATCTCA
              781 AGAAGATCCT TTGATCTTTT CTACGGGGTC TGACGCTCAG TGGAACGAAA ACTCACGTTA
              841 AGGGATTTTG GTCATGAGAT TATCAAAAAG GATCTTCACC TAGATCCTTT TAAATTAAAA
              901 ATGAAGTTTT AAATCAATCT AAAGTATATA TGAGTAAACT TGGTCTGACA GTTACCAATG
              961 CTTAATCAGT GAGGCACCTA TCTCAGCGAT CTGTCTATTT CGTTCATCCA TAGTTGCCCT
             1021 GACTCCCCGT CGTGTAGATA ACTACGATAC GGGAGGCTTT ACATCTGGGC TCCCAGTGCT
            1081 GCCATGATAC GCGAGACTCA CCGCCTTCAC CGGCCTCCAG AATTTAATTC AAGAGC
            //
            Primer: pAxCALNL_F1
            Sequence file: RDB13982_A6C7b.seq check
            >03452_13982_A6C7_pAxCALNL_F1_E05_14.ab1
                1 ATAATATATC TTATCATGTC TGCTCGAGCG GCCGCTCTAG AATTACTGCT CGTTCTTCAG
               61 CACGCGCTCC ACGAAGCTCT TGATGTACTT ACCCATTTCA TCTGGAGCGT CCTCCTGGCT
              121 GAAGTGGAGG CCCTTCACCT TCACGAACTC GGTGTTAGGG AACTTCTTAG CTCCCTCGAC
              181 AATAGCGTTG GAAAAGAACC CAGGGTCGGA CTCGATGAAC ATCTTAGGCA GATCGTCGCT
              241 GGCCCGAAGG TAGGCGTTGT AGTTGCGGAC AATCTGGACG ACGTCGGGCT TGCCTCCCTT
              301 AACGAGAGGG ATCTCGCGAG GCCAGGAGAG GGTAGGCCGT CTAACCTCGC CCTTCTCCTT
              361 GAATGGCTCC AGGTAGGCAG CGAACTCCTC AGGCTCCAGT TTCCGCATGA TCTTGCTTGG
              421 GAGCATGGTC TCGACGAAGA AGTTATTCTC AAGCACCATT TTCTCGCCCT CTTCGCTCTT
              481 GATCAGGGCG ATATCCTCCT CGATGTCAGG CCACTCGTCC CAGGACTCGA TCACGTCCAC
              541 GACACTCTCA GCATGGACGA TGGCCTTGAT CTTGTCTTGG TGCTCGTAGG AGTAGTGAAA
              601 GGCCAGACAA GCCCCCCAGT CGTGGCCCAC AAAGATGATT TTCTTTGGAA GGTTCAGCAG
              661 CTCGAACCAA GCGGTGAGGT ACTTGTAGTG ATCCAGGAGG CGATATGAGC CATTCCCGCT
              721 CTTGCCGGAC TTACCCATTC CGATCAGATC AGGGATGATG CATCTAGCCA CGGGCTCGAT
              781 GTGAGGCACG ACGTGCCTCC ACAGGTAGCT GGAGGCAGCG TTACCATGCA GAAAAATCAC
              841 GGCGTTCTCG GCGTGCTTCT CGGAATCATA GTAGTTGATG AAAGGAGTCC AGCACGTTCA
              901 TTTGCTTGCA GCGAGCCCAC CACTGAGGCC CAGTGATCAT GCGTTTGCGT TGCTCGGGGT
              961 CGTAACACTT GGAAGCATGG TGGCTAGCCT AATAGTGAGT CGTATAGTAC TCTAGCCTTA
             1021 GAGCTGTATG GACCTGGGGA GTGACACTGT GGAAGAAGGC AAGTGGATGT CAGTAGACCA
            1081 ATAGTTGCCT ATCAGAAACG GCAAAGAGAG TCC
            //

            Please visit Sequencing and PCR primers for primer information.


            References

            Original, user report and related articles

            user_report Ogawa E., p63/p51-induced onset of keratinocyte differentiation via the c-Jun N-terminal kinase pathway is counteracted by keratinocyte growth factor. J. Biol. Chem., 283, 34241-34249 (2008). PMID 18849344.

            2022.05.18

            GNP_filter3_RDBDEP_html_220516.pl