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p3AP1 (PMA) RE-TK hRluc(F) (#RDB03452)

Reporter construct of AP1 response element/PMA


Drawn by SnapGene® software
Sequence information
(Assembled from experimentally sequenced data)		 GenBank Flat File Format open
SnapGene file download
Remarks, protocol and/or map (pdf) RDB03452.pdf
Publication Unpublished bioresource
Test sheet Data Sheet open 
 
Alternative name 2055C129F
Clone info. 3' AP1 response element is cloned into the BglII site of phRL-TK, and the BglII site is killed by the cloning. The nucleotide sequence of the 3' AP1 response element is 5'-GATCCTGACTAATGACTAATGACTAAG-3'.
Comment The nucleotide sequence of the element was determined. Function of the inserted element has not been examined by the depositor.
Vector backbone phRL-TK (Plasmid)
Size of vector backbone 4.0 kb
Selectable markers Amp^r
Growth conditions 37 C, LB+Amp
Gene/insert name Artificial AP1 response element/PMA synthesized
Depositor|Developer Yokoyama, Kazunari |

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
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Terms and conditions for distribution The RECIPIENT agrees to expressly describe the acknowledgement of the Gene Engineering Division, RIKEN BRC as the source of the BIOLOGICAL RESOURCE in any publication. The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for-profit organization for a not-for-profit research.
Additional terms and conditions:
The use of the BIOLOGICAL RESOURCE is restricted to the academic researches conducted by non-profit organization. By using this material, the RECIPIENT agrees to be bound by the conditions of the limited use statement of the Promega Corporation.
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MTAに書く使用条件 リソースを使用して論文を投稿する場合、理研BRC・遺伝子材料開発室から分譲された事を記載する。本件リソースは非営利機関にのみ提供し、非営利研究にのみ利用することができる。
付加的使用条件：
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Catalog # Resource name Shipping form Fee
RDB03452 p3AP1 (PMA) RE-TK hRluc(F) DNA solution

How to cite this biological resource

Materials & Methods section:

The p3AP1 (PMA) RE-TK hRluc(F) was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB03452).

Reference section:

Further references such as user reports and related articles (go to bottom)

References

Original, user report and related articles

user_report Ogawa E., p63/p51-induced onset of keratinocyte differentiation via the c-Jun N-terminal kinase pathway is counteracted by keratinocyte growth factor. J. Biol. Chem., 283, 34241-34249 (2008). PMID 18849344. [PubMed] [Article] [RRC of NBRP]