Shuttle vector to generate recombinant adenovirus
| Catalog number | RDB03448 |
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| Resource name | pUAd5LCALNL(W1975C1F) |
| Alternative name | W1975C1F |
| Clone info. | Shuttle vector to generate rAd by the two-cosmid rescue method with pAF16Rct (RDB no. 3220) and can also be used to generate fiber-modified rAd by the COS-TPC method. pUAd5LCALNL is a derivative of pUAd5L (RDB 3444) that includes the CALNL cassette [Kanegae et al., Nucleic Acids Res. 23, 3816-21, 1995; Kanegae et al., Gene 181, 207-12, 1996] at the E1 deletion (mu 1.0 to 9.3). It can be used to generate rAd by the two-cosmid rescue method with pAF16Rct (RDB no. 3220) and can also be used to generate fiber-modified rAd by the COS-TPC method [Miyake et al., Proc. Natl. Acad. Sci. USA 93, 1320-24, 1996] without contamination of rAd that expresses wild-type fiber protein. The rAd expresses an encoded gene via the Cre-mediated control of gene expression by an expression cassette that provides the on/off-switching reporter unit and is composed of a CA promoter [Niwa et al., Gene 108, 193-99, 1991.], a loxP sequence, a stuffer sequence, and a second loxP sequence, which is followed by the SwaI cloning site and the G polyA sequence, namely, a CALNL cassette [Kanegae et al., Nucleic Acids Res. 23, 3816-21, 1995; Kanegae et al., Gene 181, 207-12, 1996]. |
| Vector backbone | Cosmid, use packaging extracts for transformation |
| Selectable markers | Amp^r |
| Gene/insert name | AdV_5 whole genome Genomic DNA |
| Depositor | Yokoyama, Kazunari | Ugai, Hideyo | |
Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
| Terms and conditions set forth by the DEPOSITOR | 1. The RECIPIENT agrees to expressly describe the acknowledgement of the Gene Engineering Division, RIKEN BRC as the source of the BIOLOGICAL RESOURCE in any publication. 2. The RECIPIENT shall send a copy of such publication to the Gene Engineering Division, RIKEN BRC. |
|---|---|
| Ordering | Please visit Information of Request for Distribution. |
| Remarks | ((Additional form)) CAG(FormE) Remember that you will be working with samples containing infectious virus. |
[open/close]| Catalog # | Resource name | Shipping form | Fee (non-profit org.) |
|---|---|---|---|
| RDB03448 | pUAd5LCALNL(W1975C1F) | DNA solution | Please contact us. |
Electronic file
Featured content
| Featured content | Cre-loxP and FLP-FRT System (English text) |
|---|---|
| Featured content | Control Vectors (English text) |
| Featured content | Empty Backbone (English text) |
| Featured content | Empty Backbone (Japanese text) |
References
| original | , J. Gene Med., 7, 1148-1157 (2005). PMID 15945121. |
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User reports and related articles (go to bottom)
This clone will be sequenced a portion and digested by restriction enzyme for examination.
References
| original | , J. Gene Med., 7, 1148-1157 (2005). PMID 15945121. |
|---|
2018.12.10
GNP_filter3_RDBDEP_html_181012.pl