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pUAd5LCALNL(W1975C23R)

Shuttle vector to generate recombinant adenovirus

Catalog number RDB03446
Resource name pUAd5LCALNL(W1975C23R)
Clone info. Shuttle vector to generate rAd by the two-cosmid rescue method with pAF16Rct (RDB no. 3220) and can also be used to generate fiber-modified rAd by the COS-TPC method.
pUAd5LCALNL is a derivative of pUAd5L (RDB 3444) that includes the CALNL cassette [Kanegae et al., Nucleic Acids Res. 23, 3816-21, 1995; Kanegae et al., Gene 181, 207-12, 1996] at the E1 deletion (mu 1.0 to 9.3). It can be used to generate rAd by the two-cosmid rescue method with pAF16Rct (RDB no. 3220) and can also be used to generate fiber-modified rAd by the COS-TPC method [Miyake et al., Proc. Natl. Acad. Sci. USA 93, 1320-24, 1996] without contamination of rAd that expresses wild-type fiber protein. The rAd expresses an encoded gene via the Cre-mediated control of gene expression by an expression cassette that provides the on/off-switching reporter unit and is composed of a CA promoter [Niwa et al., Gene 108, 193-99, 1991.], a loxP sequence, a stuffer sequence, and a second loxP sequence, which is followed by the SwaI cloning site and the G polyA sequence, namely, a CALNL cassette [Kanegae et al., Nucleic Acids Res. 23, 3816-21, 1995; Kanegae et al., Gene 181, 207-12, 1996].
Vector backbone Cosmid, use packaging extracts for transformation
Selectable markers Amp^r
Gene/insert name AdV_5 whole genome Genomic DNA
Depositor Yokoyama, Kazunari | Ugai, Hideyo |

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Terms and conditions set forth by the DEPOSITOR 1. The RECIPIENT agrees to expressly describe the acknowledgement of the Gene Engineering Division, RIKEN BRC as the source of the BIOLOGICAL RESOURCE in any publication.
2. The RECIPIENT shall send a copy of such publication to the Gene Engineering Division, RIKEN BRC.
Ordering Please visit Information of Request for Distribution. 
Remarks ((Additional form)) CAG(FormE)
Remember that you will be working with samples containing infectious virus.
Distribution information (domestic users) [open/close]


Catalog # Resource name Shipping form Fee (non-profit org.)
RDB03446 pUAd5LCALNL(W1975C23R) DNA solution Please contact us.

Ordering Information [in Japanese] [in English]

References and tips

Electronic file

Electronic file PDF RDB03446.pdf from Depositor

Featured content

Featured content Cre-loxP and FLP-FRT System (English text)
Featured content Control Vectors (English text)
Featured content Empty Backbone (English text)
Featured content Empty Backbone (Japanese text)

References

original , J. Gene Med., 7, 1148-1157 (2005). PMID 15945121.

User reports and related articles (go to bottom)


Sequence information

This clone will be sequenced a portion and digested by restriction enzyme for examination.


References

References

original , J. Gene Med., 7, 1148-1157 (2005). PMID 15945121.

2018.12.10

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