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pUAd5LCA(W1840C6R)

Shuttle vector to generate recombinant adenovirus

Catalog number RDB03445
Resource name pUAd5LCA(W1840C6R)
Alternative name W1840C6R
Clone info. Shuttle vector to generate rAd by the two-cosmid rescue method with pAF16Rct (RDB no. 3220) and can also be used to generate fiber-modified rAd by the COS-TPC method.
pUAd5LCA is a derivative of pUAd5L (RDB 3444) that includes a CAG cassette [Niwa et al., Gene 108, 193-99, 1991], harboring a unique SwaI site as the cloning site for the gene of interest, at the E1 deletion (mu 1.0 to 9.3). It can be used to generate rAd by the two-cosmid rescue method with pAF16Rct (RDB no. 3220) and can also be used to generate fiber-modified rAd by the COS-TPC method [Miyake et al., Proc. Natl. Acad. Sci. USA 93, 1320-24, 1996] without contamination of rAd that expresses wild-type fiber protein.
Vector backbone Cosmid, use packaging extracts for transformation
Selectable markers Amp^r
Gene/insert name AdV_5 whole genome Genomic DNA
Depositor|Developer Yokoyama, Kazunari | Ugai, Hideyo |

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Terms and conditions set forth by the DEPOSITOR 1. The RECIPIENT agrees to expressly describe the acknowledgement of the Gene Engineering Division, RIKEN BRC as the source of the BIOLOGICAL RESOURCE in any publication.
2. The RECIPIENT shall send a copy of such publication to the Gene Engineering Division, RIKEN BRC.
Remarks Remember that you will be working with samples containing infectious virus.
Distribution information (domestic users) [open/close]


Catalog # Resource name Availability
RDB03445 pUAd5LCA(W1840C6R) Distribution of this resource is pending.

check Please wait for results of QC test to be uploaded. This clone will be sequenced a portion for examination.
Ordering Information [in Japanese] [in English]

References and tips

Electronic file

Electronic file Remarks, protocol and/or map (pdf) RDB03445.pdf

Original reference

original , J. Gene Med., 7, 1148-1157 (2005). PMID 15945121.

Further references such as user reports and related articles (go to bottom)

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Sequence information

check Please wait for results of QC test to be uploaded. This clone will be sequenced a portion for examination.


References

Original, user report and related articles

original , J. Gene Med., 7, 1148-1157 (2005). PMID 15945121.

2021.03.25

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