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pUAd5L(W1866C2F)

Shuttle vector to generate recombinant adenovirus

Catalog number RDB03444
Resource name pUAd5L(W1866C2F)
Alternative name W1866C2F
Clone info. Shuttle vector to generate rAd by the two-cosmid rescue method with pAF16Rct (RDB no. 3220) and can also be used to generate fiber-modified rAd by the COS-TPC method. PacI linker is cloned into the SmaI site of Charomid 9-20.
This cassette cosmid for constructing rAd of the E1-substitution type, pUAd5L, is a derivative of a charomid [Saito and Stark, Proc. Natl. Acad. Sci. USA 83, 8664-68, 1996]; it includes an Ad5 genome from 0 to 76.0 mus with deletion of E1 (mu 1.0 to 9.3). The cosmid vector, which is a promoter-less vector, can be used to clone an expression cassette that is driven by the promoter of interest at the unique SwaI site in the E1 region. The pUAd5L cosmid and its derivatives can be used to generate rAd by the two-cosmid rescue method with pAF16Rct (RDB no. 3220) and can also be used to generate fiber-modified rAd by the COS-TPC method [Miyake et al., Proc. Natl. Acad. Sci. USA 93, 1320-24, 1996] without contamination of rAd that expresses wild-type fiber protein.
Vector backbone Charomid 9-20 (Cosmid, use packaging extracts for transformation)
Selectable markers Amp^r
Gene/insert name AdV_5 whole genome Genomic DNA
Depositor Yokoyama, Kazunari | Ugai, Hideyo |

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Terms and conditions set forth by the DEPOSITOR 1. The RECIPIENT agrees to expressly describe the acknowledgement of the Gene Engineering Division, RIKEN BRC as the source of the BIOLOGICAL RESOURCE in any publication.
2. The RECIPIENT shall send a copy of such publication to the Gene Engineering Division, RIKEN BRC.
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RDB03444 pUAd5L(W1866C2F) DNA solution Please contact us.

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Electronic file

Electronic file PDF RDB03444.pdf from Depositor

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References

original , J. Gene Med., 7, 1148-1157 (2005). PMID 15945121.

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Sequence information

This clone will be sequenced a portion and digested by restriction enzyme for examination.


References

References

original , J. Gene Med., 7, 1148-1157 (2005). PMID 15945121.

2018.12.10

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