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pUAd5L(W1866C2F)

Shuttle vector to generate recombinant adenovirus

Catalog number RDB03444
Resource name pUAd5L(W1866C2F)
Alternative name W1866C2F
Clone info. Shuttle vector to generate rAd by the two-cosmid rescue method with pAF16Rct (RDB no. 3220) and can also be used to generate fiber-modified rAd by the COS-TPC method. PacI linker is cloned into the SmaI site of Charomid 9-20.
This cassette cosmid for constructing rAd of the E1-substitution type, pUAd5L, is a derivative of a charomid [Saito and Stark, Proc. Natl. Acad. Sci. USA 83, 8664-68, 1996]; it includes an Ad5 genome from 0 to 76.0 mus with deletion of E1 (mu 1.0 to 9.3). The cosmid vector, which is a promoter-less vector, can be used to clone an expression cassette that is driven by the promoter of interest at the unique SwaI site in the E1 region. The pUAd5L cosmid and its derivatives can be used to generate rAd by the two-cosmid rescue method with pAF16Rct (RDB no. 3220) and can also be used to generate fiber-modified rAd by the COS-TPC method [Miyake et al., Proc. Natl. Acad. Sci. USA 93, 1320-24, 1996] without contamination of rAd that expresses wild-type fiber protein.
Vector backbone Charomid 9-20 (Cosmid, use packaging extracts for transformation)
Selectable markers Amp^r
Gene/insert name AdV_5 whole genome Genomic DNA
Depositor|Developer Yokoyama, Kazunari | Ugai, Hideyo |

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Terms and conditions set forth by the DEPOSITOR 1. The RECIPIENT agrees to expressly describe the acknowledgement of the Gene Engineering Division, RIKEN BRC as the source of the BIOLOGICAL RESOURCE in any publication.
2. The RECIPIENT shall send a copy of such publication to the Gene Engineering Division, RIKEN BRC.
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Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word]
Remarks Remember that you will be working with samples containing infectious virus.
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Catalog # Resource name Availability
RDB03444 pUAd5L(W1866C2F) Distribution of this resource is pending.

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Ordering Information [in Japanese] [in English]

References and tips

Electronic file

Electronic file Remarks, protocol and/or map (pdf) RDB03444.pdf

Original reference

original , J. Gene Med., 7, 1148-1157 (2005). PMID 15945121.

Further references such as user reports and related articles (go to bottom)

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Sequence information

check Please wait for results of QC test to be uploaded. This clone will be sequenced a portion for examination.


References

Original, user report and related articles

original , J. Gene Med., 7, 1148-1157 (2005). PMID 15945121.

2021.03.25

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