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Rat alpha-Antitryspin RRRRSL mutant (#RDB03410)

Expression vector of rat alpha-antitrypsin cDNA

Clone info. Rat alpha-antitrypsin RRRRSL mutant is an engineered Variant of rat alpha-antitrypsin that carries four mutations, Ala^349/Arg, Val^350/Arg, D^356/Arg and M^352/Arg. Expression was confirmed by depositor as follows: Immunoprecipitation and immunoblot.
Vector backbone pcDNA3 (Plasmid)
Size of vector backbone 5.4 kb
Selectable markers Amp^r (E. coli)
Gene/insert name Rat alpha1-antitrypsin cDNA
Depositor|Developer Tsuji, Akihiko |
 
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Distribution information

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Order form [Credit Card Payment] [Bank Transfer Payment] [Example of order form ]
MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ]
Please visit Information of Request for Distribution.[link] For for-profit-research purpose, please contact us. 
Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature (Tsuji, A. et al., Protein Eng. Des. Sel. 20 (4): 163-170 (2007)) are requested.
提供案内 (日本国内) [open/close]

必要書類
提供依頼書  [依頼書の記入例 ]
提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ]
手続きの概要は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。
MTAに書く使用条件 利用者は、研究成果の公表にあたって文献 (Tsuji, A. et al., Protein Eng. Des. Sel. 20 (4): 163-170 (2007)) の引用を必要とする。

Catalog # Resource name Availability Shipping form Fee (non-profit org.)
RDB03410 Rat alpha-Antitryspin RRRRSL mutant Under QC test. Please contact us. DNA solution JPY 9,460 (not-for-profit academic purpose)
plus cost of shipping containers, dry ice (if required) and shipping charge

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How to cite this biological resource

Materials & Methods section:

The Rat alpha-Antitryspin RRRRSL mutant was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB03410).

Reference section:

Tsuji, A., Kanie, H., Makise, H., Yuasa, K., Nagahama, M., Matsuda, Y., Engineering of alpha1-antitrypsin variants selective for subtilisin-like proprotein convertases PACE4 and PC6: importance of the P2' residue in stable complex formation of the serpin with proprotein convertase. Protein Eng. Des. Sel. 20 (4): 163-170 (2007). PMID 17351018. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

check Please wait for results of QC test to be uploaded. This clone will be sequenced a portion for examination.

References

Original, user report and related articles

original Tsuji, A., Engineering of alpha1-antitrypsin variants selective for subtilisin-like proprotein convertases PACE4 and PC6: importance of the P2' residue in stable complex formation of the serpin with proprotein convertase. Protein Eng. Des. Sel. 20 (4): 163-170 (2007). PMID 17351018. [link to RRC of NBRP]