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Rat alpha-Antitryspin RRRRSL mutant

Expression vector of rat alpha-antitrypsin cDNA

Catalog number RDB03410
Resource name Rat alpha-Antitryspin RRRRSL mutant
Clone info. Rat alpha-antitrypsin RRRRSL mutant is an engineered Variant of rat alpha-antitrypsin that carries four mutations, Ala^349/Arg, Val^350/Arg, D^356/Arg and M^352/Arg. Expression was confirmed by depositor as follows: Immunoprecipitation and immunoblot.
Vector backbone pcDNA3 (Plasmid)
Size of vector backbone 5.4 kb
Selectable markers Amp^r (E. coli)
Gene/insert name Rat alpha1-antitrypsin cDNA
Reference of nucleotide sequence D00675 (DDBJ accession number)
Depositor Tsuji, Akihiko |
 
Other clones in our bank Rat Serpina1 (KEGG orthology K03984) |

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature (Tsuji, A. et al., Protein Eng. Des. Sel. 20 (4): 163-170 (2007)) are requested.
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RDB03410 Rat alpha-Antitryspin RRRRSL mutant Under QC test. Please contact us. DNA solution

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Ordering Information [in Japanese] [in English]

References and tips

Electronic file

Electronic file Map RDB03410.gif

Original reference

original Tsuji, A., Engineering of alpha1-antitrypsin variants selective for subtilisin-like proprotein convertases PACE4 and PC6: importance of the P2' residue in stable complex formation of the serpin with proprotein convertase. Protein Eng. Des. Sel. 20 (4): 163-170 (2007). PMID 17351018.

Further references such as user reports and related articles (go to bottom)

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Sequence information

check Please wait for results of QC test to be uploaded. This clone will be sequenced a portion for examination.


References

Original, user report and related articles

original Tsuji, A., Engineering of alpha1-antitrypsin variants selective for subtilisin-like proprotein convertases PACE4 and PC6: importance of the P2' residue in stable complex formation of the serpin with proprotein convertase. Protein Eng. Des. Sel. 20 (4): 163-170 (2007). PMID 17351018.

2020.01.07

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