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pAxCAiLacZit

Shuttle vector to generate rAd harbouring E. coli LacZ

Catalog number RDB03380
Resource name pAxCAiLacZit
Clone info. A positive control cosmid generating recombinant adenovirus mediated by direct-transfection method after cleaving with Csp45I. The name of the generated recombinant adenovirus is AxCAiLacZ, which efficiently express beta-galactosidase.
Produce adenovirus by IT method.
Vector backbone pAxCAwtit (Cosmid, use packaging extracts for transformation)
Size of vector backbone 45 kb
Selectable markers Amp^r
Gene/insert name E. coli lacZ (beta-D-galactosidase) Genomic DNA
Depositor Saito, Izumu |
 
Other clones in our bank E. coli lacZ (KEGG orthology K01190) |

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested (Fukuda, H., Microbiol. Immunol., 50, 643-654(2006)).
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Material Transfer Agreement (MTA) [Word]
Remarks ((Additional form)) CAG(FormE)
Remember that you will be working with samples containing infectious virus.
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Catalog # Resource name Shipping form Fee (non-profit org.)
RDB03380 pAxCAiLacZit DNA solution

Ordering Information [in Japanese] [in English]

References and tips

Electronic file

Featured content

Featured content Control Vectors (English text)
Featured content Empty Backbone (English text)
Featured content Empty Backbone (Japanese text)

References

original Fukuda, H., Possible mechanism of adenovirus generation from a cloned viral genome tagged with nucleotides at its ends. Microbiol. Immunol., 50, 643-654 (2006). PMID 16924150.

User reports and related articles (go to bottom)


Sequence information

This clone will be sequenced a portion and digested by restriction enzyme for examination.


References

References

original Fukuda, H., Possible mechanism of adenovirus generation from a cloned viral genome tagged with nucleotides at its ends. Microbiol. Immunol., 50, 643-654 (2006). PMID 16924150.

2018.12.10

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