Resource data sheet
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Please review the QC test results indicated by check icon below as well as clone information before placing your order.

AxCAEGFP (forward) (#RDB03343)

Recombinant adenovirus harboring enhanced green fluorescent protein cDNA

Alternative name 196A9F3S11
Clone info. Enhanced green fluorescent protein (EGFP) is cloned into the SwaI site of pAxCAwt. (Direction:forward).
Comment Expression of proteins was not determined at the production of this virus.
Vector backbone pAxCAwt (Adenovirus)
Growth conditions DMEM+5 percent FCS+37 C + 5 percent CO2
Growth remarks 5 percent FCS is fine.
Gene/insert name Aequorea victoria GFP cDNA
Depositor|Developer DNA Bank, |
 

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Ordering forms
Order form [Credit Card Payment] [Bank Transfer Payment] [Example of order form ]
MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ]
Please visit Information of Request for Distribution.[link] 
Terms and conditions for distribution 1. The RECIPIENT agrees to use the BIOLOGICAL RESOURCE only for academic research in the non-profit organization. 2. In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested.
Additional terms and conditions:
Regarding resources containing CAG promoter: In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation for the CAG promoter (Niwa, H., Yamamura, K., Miyazaki, J., Gene 108 : 193-200, 1991) and an acknowledgment to Dr. Jun-ich Miyazaki of the Osaka University are requested.
提供案内 (日本国内) [open/close]

必要書類
提供依頼書  [依頼書の記入例 ]
提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ]
遺伝子組換え生物の受入れ確認書 [Word]
手続きの概要は、「提供申込みについて[link]」をご覧ください。
MTAに書く使用条件 1. 本件リソースは非営利機関にのみ提供し、学術研究にのみ利用することができる。 2. 謝辞の表明を必要とする。
付加的使用条件：
CAGプロモータを含むリソースについて: 利用者は、研究成果の公表にあたってCAGプロモータの文献 (Niwa, H., Yamamura, K., Miyazaki, J., Gene 108 : 193-200, 1991)を引用し、大阪大学 宮崎純一博士への謝辞の表明を必要とする。
備考 【追加提供依頼書】受入れ確認書(書式J)
組換え体提供にかかる書式が必要です。

Catalog # Resource name Shipping form Fee (non-profit org.)
RDB03343 AxCAEGFP (forward) Virus, lysate of infected cell culture JPY 32,340 plus cost of shipping containers, dry ice (if required) and shipping charge

Please review the QC test results indicated by check icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The AxCAEGFP (forward) was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB03343).

Reference section:

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB03343_A3Fjp1-1.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: pCAG-F
Sequence file: RDB03343_A3Fja.seq check
1
Primer: pAxCA_R1
Sequence file: RDB03343_A3Fjb.seq check
1
Primer: EGFP-N_F
Sequence file: RDB03343_A3Fjc.seq check
1

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

reference Kaizuka, Y., Cell membrane glycan contents are biochemical factors that constitute a kinetic barrier to viral particle uptake in a protein-nonspecific manner. bioRxiv dor: https://doi.org/10.1101/2024.06.29.601115 [link to Journal]
user_report Kaizuka, Y., Antiviral Activity of Cell Membrane-Bound Amphiphilic Polymers. Langmuir 39 (15): 5408-5417 (2023). PMID 37014318. [link to RRC of NBRP]
user_report Suzuki E., A simple method for the simultaneous detection of E1A and E1B in adenovirus stocks. Oncol. Rep., 11, 173-178 (2004). PMID 14654922. [link to RRC of NBRP]