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Please review the QC test results indicated by check icon below as well as clone information before placing your order.

AxCAEGFP (forward)

Recombinant adenovirus harboring enhanced green fluorescent protein cDNA

Catalog number RDB03343
Resource name AxCAEGFP (forward)
Alternative name 196A9F3S11
Clone info. Enhanced green fluorescent protein (EGFP) is cloned into the SwaI site of pAxCAwt. (Direction:forward).
Comment Expression of proteins was not determined at the production of this virus.
Vector backbone pAxCAwt (Adenovirus)
Growth remarks 5 percent FCS is fine.
Gene/insert name Aequorea victoria GFP cDNA
Depositor|Developer DNA Bank, |
Other clones in our bank
External Database
Aequorea victoria GFP
            Reference sequence
              
             

            Distribution information

            Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
            Terms and conditions for distribution 1. The RECIPIENT agrees to use the BIOLOGICAL RESOURCE only for academic research in the non-profit organization. 2. In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested.
            Additional terms and conditions:
            Regarding resources containing CAG promoter: In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation for the CAG promoter (Niwa, H., Yamamura, K., Miyazaki, J., Gene 108 : 193-200, 1991) and an acknowledgment to Dr. Jun-ich Miyazaki of the Osaka University are requested.
            Ordering Please visit Information of Request for Distribution.[link] 
            Order form             [Credit Card Payment]  [Bank Transfer Payment]
            Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word]
            提供案内 (日本国内) [open/close]

            提供条件 1. 本件リソースは非営利機関にのみ提供し、学術研究にのみ利用することができる。 2. 謝辞の表明を必要とする。
            付加的提供条件：
            CAGプロモータを含むリソースについて: 利用者は、研究成果の公表にあたってCAGプロモータの文献 (Niwa, H., Yamamura, K., Miyazaki, J., Gene 108 : 193-200, 1991)を引用し、大阪大学 宮崎純一博士への謝辞の表明を必要とする。
            提供依頼 手続きの詳細は、「提供申込みについて[link]」をご覧ください。
            提供依頼書 [Word]
            提供同意書 (MTA、非営利機関による非営利学術研究用)[Word]
            遺伝子組換え生物の受入れ確認書 [Word]
            備考 【追加提供依頼書】受入れ確認書(書式J)
            組換え体提供にかかる書式が必要です。

            Catalog # Resource name Shipping form Fee (non-profit org.)
            RDB03343 AxCAEGFP (forward) Virus, lysate of infected cell culture

            Please review the QC test results indicated by check icon below as well as clone information before placing your order.

            How to cite this biological resource

            Materials & Methods section:

            The AxCAEGFP (forward) was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB03343).

            Reference section:

            Further references such as user reports and related articles (go to bottom)

            References and tips

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            QC test results

            RIKEN BRC has sequenced portions of this material for quality test.
            Please review the QC test results indicated by check icon as well as clone information before placing your order.

            Test sheet RDB03343_A3Fjp1-1.pdf check

            Nucleotide sequence of a portion of this resource (if available).

            Primer: pCAG-F
            Sequence file: RDB03343_A3Fja.seq check
            1
            Primer: pAxCA_R1
            Sequence file: RDB03343_A3Fjb.seq check
            1
            Primer: EGFP-N_F
            Sequence file: RDB03343_A3Fjc.seq check
            1

            Please visit Sequencing and PCR primers for primer information.

            References

            Original, user report and related articles

            user_report Suzuki E., A simple method for the simultaneous detection of E1A and E1B in adenovirus stocks. Oncol. Rep., 11, 173-178 (2004). PMID 14654922.

            2023.05.02

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