Resource data sheet
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AxCAEGFP (forward)

Recombinant adenovirus harboring enhanced green fluorescent protein cDNA

Catalog number RDB03343
Resource name AxCAEGFP (forward)
Alternative name 196A9F3S11
Clone info. Enhanced green fluorescent protein (EGFP) is cloned into the SwaI site of pAxCAwt. (Direction:forward).
Expression of proteins was not determined at the production of this virus.
Vector backbone pAxCAwt (Adenovirus)
Gene/insert name Aequorea victoria GFP cDNA
Depositor DNA Bank, |

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Terms and conditions set forth by the DEPOSITOR 1. The RECIPIENT agrees to use the BIOLOGICAL RESOURCE only for academic research in the non-profit organization. 2. In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested.
Remarks ((Additional form)) CAG(FormE)
Please use Schedule A (GFP-A) additionally.
Distribution information (domestic users) [open/close]


Catalog # Resource name Shipping form Fee (non-profit org.)
RDB03343 AxCAEGFP (forward) Virus, lysate of infected cell culture

Ordering Information [in Japanese] [in English]

References and tips

Electronic file

Original reference

Further references such as user reports and related articles (go to bottom)

Featured content


Sequence information

RIKEN BRC has sequenced portions of this material for quality test. Primers and the results are shown below.

Data are summerized on test sheet below.

Test sheet RDB03343_A3Fj03343p1-1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: pCAG-F RDB03343_A3Fj03343a.seq
>03343_03343_A3Fj_1_pCAG-F_A01_01_ABI08.ab1
    1 TATACGGTCC TCTGGTTCCG GGGGGGACGG GGCAGGGCGG GGTTCGGCTT CTGGCAGTGT
   61 GACCGGCGGC TCTAGAGCCT CTGCTAACCA TGTTCATGCC TTCTTCTTTT TCCTACAGCT
  121 CCTGGGCAAC GTGCTGGTTA TTGTGCTGTC TCATCATTTT GGCAAAGAAT TGATTTATCG
  181 ATTTCTAGCG CTACCGGTCG CCACCATGGT GAGCAAGGGC GAGGAGCTGT TCACCGGGGT
  241 GGTGCCCATC CTGGTCGAGC TGGACGGCGA CGTAAACGGC CACAAGTTCA GCGTGTCCGG
301 CGAGGGCGAG GGCGATGCCA CCTACGGCAA GCTGACCCTG AAGTTCAAA
//
Primer: pAxCA_R1 RDB03343_A3Fj03343b.seq
>03343__03343_A3Fr_g#11_pAxCA_R1_B01_02_ABI08.ab1
    1 CATGTCATTG GCCACACCAG CCACCACCTT CTGATAGGCA GCCTGCACCT GAGGAGTGAA
   61 TTGATTATCG ATTCTAGACT AGTTTAATTA ATTTATTAAC GCTTACAATT TACGCGTTAA
  121 GATACATTGA TGAGTTTGGA CAAACCACAA CTAGAATGCA GTGAAAAAAA TGCTTTATTT
  181 GTGAAATTTG TGATGCTATT GCTTTATTTG TAACCATTAT AAGCTGCAAT AAACAAGTTA
  241 ACAACAACAA TTGCATTCAT TTTATGTTTC AGGTTCAGGG GGAGGTGTGG GAGGTTTTTT
  301 AAAGCAAGTA AAACCTCTAC AAATGTGGTA TGGCTGATTA TGATCAGTTA TCTAGATCCG
  361 GTGGATCCCG GGCCCGCGGT ACCGTCGACT GCAGAATTCG AAGCTTGAGC TCGAGATCTG
  421 AGTCCGGACT TGTACAGCTC GTCCATGCCG AGAGTGATCC CGGCGGCGGT CACGAACTCC
  481 AGCAGGACCA TGTGATCGCG CTTCTCGTTG GGGTCTTTGC TCAGGGCGGA CTGGGTGCTC
  541 AGGTAGTGGT TGTCGGGCAG CAGCACGGGG CCGTCGCCGA TGGGGGTGTT CTGCTGGTAG
  601 TGGTCGGCGA GCTGCACGCT GCCGTCCTCG ATGTTGTGGC GGATCTTGAA GTTCACCTTG
661 ATGCCGTTCT TCTGCTTGTC GGCCATGATA TAGACGTTGT GGCTGTTGTA GTTGTACT
//
Primer: EGFP-N_F RDB03343_A3Fj03343c.seq
>03343__03343_A3Fr_g#11_EGFP-N_F_A01_01_ABI08.ab1
    1 CGTCGGCGAT GTCTGGCGAG GTGCGAGGGC GATGCCGCCT ACGGCAAGCT GACCCTGAAG
   61 TTCATCTGCA CCACCGGCAA GCTGCCCGTG CCCTGGCCCA CCCTCGTGAC CACCCTGACC
  121 TACGGCGTGC AGTGCTTCAG CCGCTACCCC GACCACATGA AGCAGCACGA CTTCTTCAAG
  181 TCCGCCATGC CCGAAGGCTA CGTCCAGGAG CGCACCATCT TCTTCAAGGA CGACGGCAAC
  241 TACAAGACCC GCGCCGAGGT GAAGTTCGAG GGCGACACCC TGGTGAACCG CATCGAGCTG
  301 AAGGGCATCG ACTTCAAGGA GGACGGCAAC ATCCTGGGGC ACAAGCTGGA GTACAACTAC
  361 AACAGCCACA ACGTCTATAT CATGGCCGAC AAGCAGAAGA ACGGCATCAA GGTGAACTTC
  421 AAGATCCGCC ACAACATCGA GGACGGCAGC GTGCAGCTCG CCGACCACTA CCAGCAGAAC
  481 ACCCCCATCG GCGACGGCCC CGTGCTGCTG CCCGACAACC ACTACCTGAG CACCCAGTCC
  541 GCCCTGAGCA AAGACCCCAA CGAGAAGCGC GATCACATGG TCCTGCTGGA GTTCGTGACC
  601 GCCGCCGGGA TCACTCTCGG CATGGACGAG CTGTACAAGT CCGGACTCAG ATCTCGAGCT
  661 CAAGCTTCGA ATTCTGCAGT CGACGGTACC GCGGGCCCGG GGATCCACCG GGATCTAGAT
721 AA
//

Please visit Sequencing and PCR primers for primer information.


References

Original, user report and related articles

user report Suzuki E., A simple method for the simultaneous detection of E1A and E1B in adenovirus stocks. Oncol. Rep., 11, 173-178 (2004). PMID 14654922.

2019.07.16

GNP_filter3_RDBDEP_html_190432.pl