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pAxCALNLrAAT-RXXR (forward) (#RDB03198)

Shuttle vector to generate rAd harbouring human alpha1-antitrypsin cDNA

Alternative name 1157 C2
Clone info. Shuttle vector to generate recombinant adenovirus harbouring human alpha1-antitrypsin cDNA. RXXR mutant form.
Comment Cre-recombinase is required to remove LNL stuffer sequence. Use COS-TPC method for the production of adenovirus.
Vector backbone pAxCALNLw (Cosmid, use packaging extracts for transforming E. coli host)
Selectable markers Amp^r
Gene/insert name Rat alpha1-antitrypsin cDNA
Depositor|Developer DNA Bank, |
 

Distribution information

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MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ]
Please visit Information of Request for Distribution.[link] 
Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature(s) designated by the DEPOSITOR is requested. (Tsuji et al. J. Biochem, 126, 591-603 (1999); Tsuji et al. Protein Engng., 15(2), 123-130 (2002).) The RECIPIENT agrees to use the BIOLOGICAL RESOURCE only for not-for-profit research in the not-for-profit organization.
Additional terms and conditions:
Regarding resources containing CAG promoter: In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation for the CAG promoter (Niwa, H., Yamamura, K., Miyazaki, J., Gene 108 : 193-200, 1991) and an acknowledgment to Dr. Jun-ich Miyazaki of the Osaka University are requested.
Remarks Remember that you will be working with samples containing infectious virus.
提供案内 (日本国内) [open/close]

必要書類
提供依頼書  [依頼書の記入例 ]
提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ]
手続きの概要は、「提供申込みについて[link]」をご覧ください。
MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する(Tsuji et al. J. Biochem, 126, 591-603 (1999); Tsuji et al. Protein Engng., 15(2), 123-130 (2002).)。 本件リソースは非営利機関にのみ提供し、非営利研究にのみ利用することができる。
MTAに書く付加的使用条件：
CAGプロモータを含むリソースについて: 利用者は、研究成果の公表にあたってCAGプロモータの文献 (Niwa, H., Yamamura, K., Miyazaki, J., Gene 108 : 193-200, 1991)を引用し、大阪大学 宮崎純一博士への謝辞の表明を必要とする。
備考 このリソースはウイルス粒子産生用のため、取扱いに注意が必要です。

Catalog # Resource name Availability Shipping form Fee (non-profit org.)
RDB03198 pAxCALNLrAAT-RXXR (forward) Under QC test. Please contact us. DNA solution JPY 9,460 (not-for-profit academic purpose)
plus cost of shipping containers, dry ice (if required) and shipping charge

check Please wait for results of QC test to be uploaded. This clone will be sequenced a portion for examination.

How to cite this biological resource

Materials & Methods section:

The pAxCALNLrAAT-RXXR (forward) was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB03198).

Reference section:

Further references such as user reports and related articles (go to bottom)

QC test results

check Please wait for results of QC test to be uploaded. This clone will be sequenced a portion for examination.

References

Original, user report and related articles

reference Tsuji, A., Development of selectivity of alpha1-antitrypsin variant by mutagenesis in its reactive site loop against proprotein convertase. A crucial role of the P4 arginine in PACE4 inhibition. Protein Eng. 15 (2): 123-130 (2002). PMID 11917148. [link to RRC of NBRP]
reference Tsuji, A., Inactivation of proprotein convertase, PACE4, by alpha1-antitrypsin Portland (alpha1-PDX), a blocker of proteolytic activation of bone morphogenetic protein during embryogenesis: evidence that PACE4 is able to form an SDS-stable acyl intermediate with alpha1-PDX. J. Biochem, 126, 591-603 (1999). PMID 10467177.