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pAxCAwtit (#RDB03121)

A dual cassette for constructing recombinant adenovirus containing CAG promoter


Drawn by SnapGene® software
Sequence information
(Assembled from experimentally sequenced data)		 GenBank Flat File Format open
SnapGene file download
Remarks, protocol and/or map (pdf) RDB03121.pdf
Publication Fukuda, H., Microbiol. Immunol., 50, 643-654 (2006). PMID 16924150. [PubMed] [Article] [RRC of NBRP]
Publication Terashima, M., Exp. Med., 21, 931-936 (2003).
Test sheet Data Sheet open 
 
Clone info. A dual cassette for constructing recombinant adenovirus containing CAG promoter improved from pAxCAwt. Please note that pAxCAwtit has been improved as pAxCAwtit2 (RDB05213), which contains a Csp45I site and an additional PacI site for linearizing the cosmid vector by restriction enzymes.
Comment The pAxCAwtit cosmid is a derivative of pAxcwit (RDB 3120) and can be used to clone a CAG cassette [Niwa et al., Gene 108, 193-99, 1991], harboring a unique SwaI site as the cloning site for the gene of interest, at the E1 deletion. Upon transfection of HEK293 cells, the pAxCAwtit cosmid, digested with Csp45I, can generate rAd. The pAxCAwtit cosmid can also be used to generate rAd by the COS-TPC method [Miyake et al., Proc. Natl. Acad. Sci. USA 93, 1320-24, 1996].
Vector backbone charomid 9-11 (Cosmid, use packaging extracts for transforming E. coli host)
Size of vector backbone 11 kb
Selectable markers Amp^r
Growth conditions 37C, LB+Amp
Gene/insert name AdV_5 E1, E3-deleted adenovirus 5 genome Genomic DNA
Depositor|Developer Saito, Izumu |

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Ordering forms
Order form [Credit Card Payment] [Bank Transfer Payment] [Example of order form ]
MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ]
Please visit Information of Request for Distribution.[link] For for-profit-research purpose, please contact us. 
Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested (Terashima, M., Exp. Med., 21, 931-936(2003)).
Additional terms and conditions:
Regarding resources containing CAG promoter: In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation for the CAG promoter (Niwa, H., Yamamura, K., Miyazaki, J., Gene 108 : 193-200, 1991) and an acknowledgment to Dr. Jun-ich Miyazaki of the Osaka University are requested.
Remarks Remember that you will be working with samples containing infectious virus.
提供案内 (日本国内) [open/close]

必要書類
提供依頼書  [依頼書の記入例 ]
提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ]
手続きの概要は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。
MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Terashima, M., Exp. Med., 21, 931-936(2003))。
付加的使用条件：
CAGプロモータを含むリソースについて: 利用者は、研究成果の公表にあたってCAGプロモータの文献 (Niwa, H., Yamamura, K., Miyazaki, J., Gene 108 : 193-200, 1991)を引用し、大阪大学 宮崎純一博士への謝辞の表明を必要とする。
備考 このリソースはウイルス粒子産生用のため、取扱いに注意が必要です。

Catalog # Resource name Shipping form Fee
RDB03121 pAxCAwtit DNA solution

How to cite this biological resource

Materials & Methods section:

The pAxCAwtit was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB03121).

Reference section:

Fukuda, H., Terashima, M., Koshikawa, M., Kanegae, Y., Saito, I., Possible mechanism of adenovirus generation from a cloned viral genome tagged with nucleotides at its ends. Microbiol. Immunol., 50, 643-654 (2006). PMID 16924150. [PubMed] [Article] [RRC of NBRP]
Terashima, M., Kondo, S., Kanegae, Y., Saito, I., [Japanese text] Exp. Med., 21, 931-936 (2003).

Further references such as user reports and related articles (go to bottom)

References

Original, user report and related articles

original Fukuda, H., Possible mechanism of adenovirus generation from a cloned viral genome tagged with nucleotides at its ends. Microbiol. Immunol., 50, 643-654 (2006). PMID 16924150. [PubMed] [Article] [RRC of NBRP]
original Terashima, M., [Japanese text] Exp. Med., 21, 931-936 (2003).
user_report Heng, J.I., The zinc finger transcription factor RP58 negatively regulates Rnd2 for the control of neuronal migration during cerebral cortical development. Cereb. Cortex 25 (3): 806-816 (2015). PMID 24084125. [PubMed] [Article] [RRC of NBRP]
user_report Ikeno Y., Secretory phospholipases A2 induce neurite outgrowth in PC12 cells through lysophosphatidylcholine generation and activation of G2A receptor. J. Biol. Chem., 280, 28044-28052, (2005). PMID 15927955. [PubMed] [Article] [RRC of NBRP]