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pAxCAwtit

A dual cassette for constructing recombinant adenovirus containing CAG promoter

Catalog number RDB03121
Resource name pAxCAwtit
Clone info. A dual cassette for constructing recombinant adenovirus containing CAG promoter. Improved version of pAxCAwt.
The pAxCAwtit cosmid is a derivative of pAxcwit (RDB 3120) and can be used to clone a CAG cassette [Niwa et al., Gene 108, 193-99, 1991], harboring a unique SwaI site as the cloning site for the gene of interest, at the E1 deletion. Upon transfection of HEK293 cells, the pAxCAwtit cosmid, digested with Csp45I, can generate rAd. The pAxCAwtit cosmid can also be used to generate rAd by the COS-TPC method [Miyake et al., Proc. Natl. Acad. Sci. USA 93, 1320-24, 1996].
Vector backbone charomid 9-11 (Cosmid, use packaging extracts for transformation)
Size of vector backbone 11 kb
Selectable markers Amp^r
Gene/insert name AdV_5 E1, E3-deleted adenovirus 5 genome Genomic DNA
Depositor Saito, Izumu |

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested (Terashima, M., Exp. Med., 21, 931-936(2003)).
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Material Transfer Agreement (MTA) [Word]
Remarks ((Additional form)) CAG(FormE)
Remember that you will be working with samples containing infectious virus.
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RDB03121 pAxCAwtit DNA solution

Ordering Information [in Japanese] [in English]

References and tips

Electronic file

Electronic file Sequence RDB03121u.seq from Depositor
Electronic file Sequence RDB03121w.seq from Depositor
Electronic file Sequence RDB03121v.seq from Depositor
Electronic file Sequence RDB03121y.seq from Depositor
Electronic file Sequence RDB03121x.seq from Depositor
Electronic file Sequence RDB03121z.seq from Depositor
Electronic file Map RDB03121.gif from Depositor
Electronic file PDF RDB03121.pdf from Depositor

Featured content

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Featured content Empty Backbone (Japanese text)

References

original Fukuda, H., Possible mechanism of adenovirus generation from a cloned viral genome tagged with nucleotides at its ends. Microbiol. Immunol., 50, 643-654 (2006). PMID 16924150.
original Terashima, M., [Japanese text] Exp. Med., 21, 931-936 (2003).

User reports and related articles (go to bottom)


Sequence information

This clone will be sequenced a portion and digested by restriction enzyme for examination.


References

References

original Fukuda, H., Possible mechanism of adenovirus generation from a cloned viral genome tagged with nucleotides at its ends. Microbiol. Immunol., 50, 643-654 (2006). PMID 16924150.
original Terashima, M., [Japanese text] Exp. Med., 21, 931-936 (2003).
user report Heng, J.I., The zinc finger transcription factor RP58 negatively regulates Rnd2 for the control of neuronal migration during cerebral cortical development. Cereb. Cortex 25 (3): 806-816 (2015). PMID 24084125.
user report Ikeno Y., Secretory phospholipases A2 induce neurite outgrowth in PC12 cells through lysophosphatidylcholine generation and activation of G2A receptor. J. Biol. Chem., 280, 28044-28052, (2005). PMID 15927955.

2018.12.10

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