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pAxcwit

A dual cassette for constructing recombinant adenovirus

Catalog number RDB03120
Resource name pAxcwit
Clone info. A dual cassette for constructing recombinant adenovirus. Improved version of pAxcw containing intact termini of Ad DNA.
The pAxcwit cosmid is a derivative of pAxcw (RDB 917) and includes the Ad5 genome from 0 to 100 map units (mus). The cosmid vector, which is a promoter-less vector, can be used to clone an expression cassette that is driven by the promoter of interest at the unique SwaI site in the E1 region. The pAxcwit cosmid, digested with Csp45I, can generate rAd upon transfection of HEK293 cells. The Axcwit cosmid can also be used to generate rAd by the COS-TPC method [Miyake et al., Proc. Natl. Acad. Sci. USA 93, 1320-24, 1996].
Vector backbone charomid 9-11 (Cosmid, use packaging extracts for transformation)
Size of vector backbone 11 kb
Selectable markers Amp^r
Gene/insert name AdV_5 E1, E3-deleted adenovirus 5 genome Genomic DNA
Depositor|Developer Saito, Izumu |

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested (Terashima, M., Exp. Med., 21, 931-936(2003)).
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Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word]
Remarks Remember that you will be working with samples containing infectious virus.
Distribution information (domestic users) [open/close]


Catalog # Resource name Availability Shipping form Fee (non-profit org.)
RDB03120 pAxcwit Under QC test. Please contact us. DNA solution

check Please wait for results of QC test to be uploaded. This clone will be sequenced a portion for examination.
Ordering Information [in Japanese] [in English]

References and tips

Electronic file

Electronic file Sequence RDB03120y.seq
Electronic file Sequence RDB03120z.seq
Electronic file Map RDB03120.gif
Electronic file Remarks, protocol and/or map (pdf) RDB03120.pdf

Original reference

original Fukuda, H., Possible mechanism of adenovirus generation from a cloned viral genome tagged with nucleotides at its ends. Microbiol. Immunol., 50, 643-654 (2006). PMID 16924150.
original Terashima, M., [Japanese text] Exp. Med., 21, 931-936 (2003).

Further references such as user reports and related articles (go to bottom)

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Sequence information

check Please wait for results of QC test to be uploaded. This clone will be sequenced a portion for examination.


References

Original, user report and related articles

original Fukuda, H., Possible mechanism of adenovirus generation from a cloned viral genome tagged with nucleotides at its ends. Microbiol. Immunol., 50, 643-654 (2006). PMID 16924150.
original Terashima, M., [Japanese text] Exp. Med., 21, 931-936 (2003).
user report Kojima, Y., Oncolytic gene therapy combined with double suicide genes for human bile duct cancer in nude mouse models. J. Surg. Res. 157 (1): e63-70 (2009). PMID 19345377.

2021.03.25

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