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pAxcwit

A dual cassette for constructing recombinant adenovirus

Catalog number RDB03120
Resource name pAxcwit
Clone info. A dual cassette for constructing recombinant adenovirus improved from pAxcw. Please note that pAxcwit has been improved as pAxcwit2 (RDB05212), which contains a Csp45I site and an additional PacI site for linearizing the cosmid vector by restriction enzymes.
Comment The pAxcwit cosmid is a derivative of pAxcw (RDB 917) and includes the Ad5 genome from 0 to 100 map units (mus). The cosmid vector, which is a promoter-less vector, can be used to clone an expression cassette that is driven by the promoter of interest at the unique SwaI site in the E1 region. The pAxcwit cosmid, digested with Csp45I, can generate rAd upon transfection of HEK293 cells. The Axcwit cosmid can also be used to generate rAd by the COS-TPC method [Miyake et al., Proc. Natl. Acad. Sci. USA 93, 1320-24, 1996].
Vector backbone charomid 9-11 (Cosmid, use packaging extracts for transformation)
Size of vector backbone 11 kb
Selectable markers Amp^r
Gene/insert name AdV_5 E1, E3-deleted adenovirus 5 genome Genomic DNA
Depositor|Developer Saito, Izumu |
Other clones in our bank

External Database
AdV_5 E1, E3-deleted adenovirus 5 genome

            Reference sequence
              

            Distribution information

            Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
            Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested (Terashima, M., Exp. Med., 21, 931-936(2003)).
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            Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word]
            Remarks Remember that you will be working with samples containing infectious virus.
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            提供条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Terashima, M., Exp. Med., 21, 931-936(2003))。
            提供依頼 手続きの詳細は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。
            提供依頼書 [Word]
            提供同意書 (MTA、非営利機関による非営利学術研究用)[Word]
            備考 このリソースはウイルス粒子産生用のため、取扱いに注意が必要です。

            Catalog # Resource name Availability Shipping form Fee (non-profit org.)
            RDB03120 pAxcwit Under QC test. Please contact us. DNA solution

            check Please wait for results of QC test to be uploaded. This clone will be sequenced a portion for examination.

            References and tips

            Electronic file

            Sequence RDB03120y.seq
            Sequence RDB03120z.seq
            Remarks, protocol and/or map (gif) RDB03120.gif
            Remarks, protocol and/or map (pdf) RDB03120.pdf

            Original reference

            original Fukuda, H., Possible mechanism of adenovirus generation from a cloned viral genome tagged with nucleotides at its ends. Microbiol. Immunol., 50, 643-654 (2006). PMID 16924150.
            original Terashima, M., [Japanese text] Exp. Med., 21, 931-936 (2003).

            Further references such as user reports and related articles (go to bottom)

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            Sequence information

            check Please wait for results of QC test to be uploaded. This clone will be sequenced a portion for examination.


            References

            Original, user report and related articles

            original Fukuda, H., Possible mechanism of adenovirus generation from a cloned viral genome tagged with nucleotides at its ends. Microbiol. Immunol., 50, 643-654 (2006). PMID 16924150.
            original Terashima, M., [Japanese text] Exp. Med., 21, 931-936 (2003).
            user_report Kojima, Y., Oncolytic gene therapy combined with double suicide genes for human bile duct cancer in nude mouse models. J. Surg. Res. 157 (1): e63-70 (2009). PMID 19345377.

            2022.05.18

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