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pMY

Complete genome of JC virus, archetype

Catalog number RDB03032
Resource name pMY
Clone info. Complete genome of JC virus, archetype.
Comment This clone contains whole genome of AB038250.1 (4,299nt to 5,121nt and 1nt to 4,298nt) in BamHI site of pUC19 by quality test.
Vector backbone pUC19 (Plasmid)
Size of vector backbone 2,690 bp
Selectable markers Amp^r
Gene/insert name Hum_Polyoma JCV genome Genomic DNA
Depositor|Developer Yogo, Yoshiaki |
Other clones in our bank

External Database
Hum_Polyoma JCV genome

            Reference sequence
              

            Distribution information

            Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
            Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature(s) designated by the DEPOSITOR is requested.
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            RDB03032 pMY DNA solution

            Please review the QC test results indicated by check icon below as well as clone information before placing your order.

            References and tips

            Electronic file

            Remarks, protocol and/or map (gif) RDB03032.gif

            Original reference

            original Yogo, Y., Isolation of a possible archetypal JC virus DNA sequence from nonimmunocompromised individuals. J. Virol., 64, 3139-3143 (1990). PMID 2159570.

            Further references such as user reports and related articles (go to bottom)

            Featured content


            Sequence information

            RIKEN BRC has sequenced portions of this material for quality test.
            Please review the QC test results indicated by check icon as well as clone information before placing your order.

            Test sheet RDB17355_A9F3p1-2.pdf check

            Nucleotide sequence of a portion of this resource (if available).

            Primer: pUC-CAPbs_up_F
            Region: CAP binding site,lac pro,lacO,insert 5'
            Sequence file: RDB17355_A9F3g.seq check
            >03032_17355_A9F3_2_pUC-CAPbs_up_F_C04_07_ABI24.ab1
                1 NNNNNNNNNN NNNNNNNCNC NNCGCNNNAA TGTGAGTTAG CTCACTCATT AGGCACCCCA
               61 GGCTTTACAC TTTATGCTTC CGGCTCGTAT GTTGTGTGGA ATTGTGAGCG GATAACAATT
              121 TCACACAGGA AACAGCTATG ACCATGATTA CGCCAAGCTT GCATGCCTGC AGGTCGACTC
              181 TAGAGGATCC TGTGTTTTCA TCATCACTGG CAAACATTTC TTCATGGCAA AACAGGTCTT
              241 CATCCCACTT CTCATTAAAT GTATTCCACC AGGATTCCCA TTCATCTGTT CCATAGGTTG
              301 GCACCTAAAA ACAAAAAATT AAGTTTATTG TAAAAAACAA AATGCCCTGC AAAAGAAAAA
              361 TTGTGGTTTA CCTTAAAGCT TTAGATCCCT GTAGGGGGTG TCTCCAAGAA CCTTCTCCCA
              421 GCAATGAAGA GCTTCTTGGG TTAAGTCACA CCCAAACCAT TGTCTGAAGC AATCAAAGCA
              481 ATAGCAATCT ATCCACACAA GTGGGCTGCT TCTTAAAAAT TTTCTGTTTC TATGCCTTAA
              541 TTTTAGCATG CACATTAAAC AGGGGCAATG CACTGAAGGA TTAGTGGCAC AATTAGGCCA
              601 TTCCTTGCAA TAAAGGGTAT CAGAATTAGG AGGAAAATCA CAACCAACCT CTGAACTATT
              661 CCATGTACCA AAATCAGGCT GATGAGCAAC TTTTACACCT TGTTCCATTT TTTTATATAA
              721 AAAATTCATT CTCTTCATTT TGTCTTCGTC CCCACCTTTA TCAGGNTGAA GTTCTTTGCA
              781 TTTTTTCAGA TAAGCTTTTC TCATGACAGG AATGTTTCNN CATGCAGATC TATCAANGCC
              841 TAATAAATCC NTGANCTCCA NGGATTCCTC CCNATTCAGC ACTTTNGTCC ATTTTTTGCT
              901 TTTTGTAGCA AAAAATTANN GCAAAAANNN NAAAAANNAN NNNNNNTTCC NNGGCCNCCN
            961 AAAAAAGCNN NNNNNCCCNN ANNANTTNNG ANTAANNTNN GNNNGNNNNN NNNNN
            //
            Primer: pUC-M13_-100
            Region: insert 3'
            Sequence file: RDB17355_A9F3h.seq check
            >03032_17355_B1HpF_pUC-M13_-100_H02_23_ABI24.ab1
                1 NNNNNNNNNN NANNNNGGNA NGCCNGGGNT TTCCCNGTCA CGACGTTGTA AAACGACGGC
               61 CAGTGAATTC GAGCTCGGTA CCCGGGGATC CCAACACTCT ACCCCACCTA AGAAGAAAAA
              121 AAAGGTAGAA GACCCTAAAG ACTTTCCTGT AGATCTGCAT GCATTCCTCA GTCAAGCTGT
              181 GTTTAGTAAT AGAACTGTTG CTTCTTTTGC TGTGTATACC ACTAAAGAAA AAGCTCAAAT
              241 TTTATATAAG AAACTTATGG AAAAATATTC TGTAACCTTT ATAAGTAGAC ATGGTTTTGG
              301 GGGTCATAAT ATCTTGTTTT TTCTAACACC ACATAGACAT AGAGTTTCAG CAATTAATAA
              361 TTACTGTCAA AAACTATGTA CCTTTAGTTT TTTAATTTGT AAAGGTGTGA ATAAGGAATA
              421 CTTGTTTTAC AGTGCCTTGT GTAGACAGCC ATATGCAGTA GTGGAAGAAA GTATTCAGGG
              481 GGGCCTTAAG GAGCATGACT TTAACCCAGA AGAACCAGAA GAAACTAAGC AGGTTTCATG
              541 GAAATTAGTT ACACAGTATG CTTTGGACAC CAAGTGTGAG GATGTTTTCT TGCTTATGGG
              601 CATGTACTTA GACTTTCAGG AAAACCCTTT GCNATGCAAA AAATGNGAAA AAAANGNNCA
              661 GCCAAATCAC NTNNACCATC ATGAAAAGCA CTATTATNNN GNCNNANTTT TNNNNNANNN
            721 NNAAANNNNA AAAANNNNNT NNNNNNNNNN
            //
            Primer: JCsp_f1
            Region: insert mid
            Sequence file: RDB17355_A9F3i.seq check
            >03032_17355_A9F3_2_JCsp_f1_B01_04_ABI24.ab1
                1 NNNNNNNNNN NNNNNNNGGN CCCNNCAAAA AGAANAGGAG AAAGGAAGGA NCCCGTGCAA
               61 GTTCCAAAAC TTCTTATAAG AGGAGGAGTA GAAGTTCTAG AAGTTAAAAC TGGGGTTGAC
              121 TCAATTACAG AGGTAGAATG CTTTTTAACT CCAGAAATGG GTGACCCAGA TGAGCATCTT
              181 AGGGGTTTTA GTAAGTCAAT TTCTATATCA GATACATTTG AAAGTGACTC CCCAAATAAG
              241 GACATGCTTC CTTGTTACAG TGTGGCCAGA ATTCCACTAC CCAATCTAAA TGAGGATCTA
              301 ACCTGTGGAA ATATACTAAT GTGGGAGGCT GTGACCTTAA AAACTGAGGT TATAGGGGTG
              361 ACAACTTTGA TGAATGTGCA CTCTAATGGT CAAGCAACTC ATGACAATGG TGCAGGAAAG
              421 CCAGTGCAGG GCACCAGCTT TCATTTTTTT TCTGTTGGGG GGGAGGCTTT AGAATTACAG
              481 GGGGTGGTTT TTAATTACAG AACAAAGTAC CCAGATGGAA CAATTTTTCC AAAGAATGCA
              541 ACAGTGCAAT CTCAAGTAAT GAACACAGAG CACAAGGCGT ACCTAGATAA GAACAAAGCA
              601 TATCCTGTTG AATGTTGGGT TCCTGATCCC ACCAGAAATG AAAACACAAG ATATTTTGGG
              661 ACACTAACAG GAGGAGAAAA TGTTCCTCCA GTTCTTCATA TAACAAATAC TGCNACAACA
              721 GTGCTGCTTG ATGAATTTGG TGTTGGGCCA CTTTGCNAAG GTGACAACTT GTATTTGTCA
              781 GCTGTTGATG TTTGTGGAAT GTTTACTAAC AGATCTGGTT CCCNNCANTG GAGAGGANTG
              841 TCCNGATATT TNNNNGTTCA GCTCANAAAA AGGANGGTTA AAAACCCCTA CCCNATTTCT
              901 TNCCTTCTTA CTGATTTGAN TNNNANAANN NNCCCTNNAN TTGANGGGNA NCCTANNNAN
            961 GGNANNNNNG CNNNNNNNNN N
            //
            Primer: JCsp_R2
            Region: insert mid
            Sequence file: RDB17355_A9F3j.seq check
            >03032_17355_A9F3_2_JCsp_R2_B03_06_ABI24.ab1
                1 NNNNNNNNNN NNNNNNNNNT NNNNCTTTAT TAAAATGTAC TGCATATTAC AATAAAGGCT
               61 TTTGGTTACA GCATCTTTGT TTGCAACTGT CCATATCTGT CAACATATCT CATCATGTCT
              121 GGGTCCCCTG GAAGTTCCTC TGTCCCCTCA AAAACTCTAA CCTCCTCTAC CTGAGCATCC
              181 ATACCATACA TAGGCTGCCC ATCAACTCTA GGGGTCCTTC TGTTAATCAA ATCAGTAAGA
              241 AGGAAAGAAA TTGGGTAGGG GTTTTTAACC CTCCTTTTTC TGAGCTGAAC CTTAAAATAT
              301 CTGGACAGTC CTCTCCACTG CTGGGAACCA GATCTGTTAG TAAACATTCC ACAAACATCA
              361 ACAGCTGACA AATACAAGTT GTCACCTTTG CAAAGTGGCC CAACACCAAA TTCATCAAGC
              421 AGCACTGTTG TGGCAGTATT TGTTATATGA AGAACTGGAG GAACATTTTC TCCTCCTGTT
              481 AGTGTCCCAA AATATCTTGT GTTTTCATTT CTGGTGGGAT CAGGAACCCA ACATTCAACA
              541 GGATATGCTT TGTTCTTATC TAGGTACGCC TTGTGCTCTG TGTTCATTAC TTGAGATTGC
              601 ACTGTTGCAT TCTTTGGAAA AATTGTTCCA TCTGGGTACT TTGTTCTGTA ATTAAAAACC
              661 ACCCCCTGTA ATTCTAAAGC CTCCCCCCCA ACAGAAAAAA AATGAAAGCT GGTGCCCTGC
              721 ACTGGCTTTC CTGCACCATT GTCATGAGTT GCTTGACCAT TAGAGTGCAC ATTCATCAAA
              781 GTTGTCACCC CTATAACCTC AGTTTTTAAG GTCACAGCCT CCCACATTAG TATATTTCCA
              841 CANGTTAGAT CCTCATTTAG ATTGGGTAGT NNAATTCTGG NCACACTGTA ACNAGGAANC
              901 ATGTCCTTAT TTGGGGANTC ANTTTCAAAT GTATCTGATA TAGAAATTGA CTTACTAAAA
            961 CCCCNNANAT GCTCATCNNG GNNACCCATT TNNNGNNNNT NNAAANNNNN NNNNN
            //
            Primer: Jcsp_f4
            Region: insert mid
            Sequence file: RDB17355_A9F3k.seq check
            >03032_17355_A9F3_2_JCsp_f4_F01_16_ABI24.ab1
                1 NNNNNNNNNN NNNNNNNCNN NTTNNGNACT TTGTCNNTTT TTGCTTTTTG TAGCAAAAAA
               61 TTAGTGCAAA AAAGGGAAAA ACAAGGGAAT TTCCCTGGCC TCCTAAAAAG CCTCCACGCC
              121 CTTACTACTT CTGAGTAAGC TTGGAGGCGG AGGCGGCCTC GGCCTCCTGT ATATATAAAA
              181 AAAAGGGAAG GTAGGGAGGA GCTGGCTAAA ACTGGATGGC TGCCAGCCAA GCATGAGCTC
              241 ATACCTAGGG AGCCAACCAG CAGACAGCCA GAGGGAGCCC TGGCTGCATG CCACTGGCAG
              301 TTATAGTGAA ACCACTCCCA TAGTCCTTAA TCACAAGTAA ACAAAGCACA AGGGGAAGTG
              361 GAAAGCAGCC AAGGGAACAT GTTTTGCGAG CCAGAGCTGT TTTGGCTTGT CACCAGCTGG
              421 CCATGGTTCT TCGCCAGCTG TCACGTAAGG CTTCTGTGAA AGTTAGTAAA ACCTGGAGTG
              481 GAACTAAAAA AAGAGCTCAA AGGATTTTAA TTTTTTTGTT AGAATTTTTG CTGGATTTTT
              541 GCACAGGTGA AGACAGTGTA GACGGGAAAA AAAGGCAGAA ACACAGTGGT TTGACTCAGC
              601 AGACATACAG TGCTTTGCCT GAACCAAAAG CTACATAGGT AAGTAATGTT TTTTTTTGTG
              661 TTTTCAGGTT CATGGGTGCC GCACTTGCAC TTTTGGGGGA NCTAGTTGCT ACTGTTTCTG
              721 ANGCTGCTGC TGCCACAGGA TTTTCAGTAG CTGAAATTGC TGCTGGAGAN GCTGCTGCTA
              781 CTATAGAAGT TGAAATTGCA TCCCTTGCTA CTGTAGANGG GNNTACAAGT ACCTCTGANG
              841 CTATANCTGC AATAGGCCTT ACTCCTGNNA CATATGCTGT AANTACNNGA GCNNCNGGGG
              901 GCTGTANCNN GGNTTTNNTG NNNTNGGTTC AAACTNNNAN NNGGTGGNNG NGCTNTNGCT
              961 CANTTGGGNN NTNNANTTTT TGCTGNNNNG GNATCATAAA GTTTNNNNNN TNNGGNNTTT
            1021 TNANCANNNN NNNNNN
            //

            Please visit Sequencing and PCR primers for primer information.


            References

            Original, user report and related articles

            original Yogo, Y., Isolation of a possible archetypal JC virus DNA sequence from nonimmunocompromised individuals. J. Virol., 64, 3139-3143 (1990). PMID 2159570.

            2022.05.18

            GNP_filter3_RDBDEP_html_220516.pl