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Please review the QC test results indicated by check icon below as well as clone information before placing your order.

pCY

Complete genome of JC virus, archetype

Catalog number RDB03031
Resource name pCY
Clone info. Complete genome of JC virus, archetype.
Comment This clone contains whole genome of AB038249.1 (4,298nt to 1nt and 5,120nt to 4,299nt) in BamHI site of pUC19 by quality test. Known differences: A to C substitution (Gly, synonym) at 2446 nt of AB038249.1.
Vector backbone pUC19 (Plasmid)
Size of vector backbone 2,690 bp
Selectable markers Amp^r
Gene/insert name Hum_Polyoma JCV genome Genomic DNA
Reference of insert sequence AB038249 (DDBJ accession number)
Depositor|Developer Yogo, Yoshiaki |
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External Database
Hum_Polyoma JCV genome

            Reference sequence
              

            Distribution information

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            RDB03031 pCY DNA solution

            Please review the QC test results indicated by check icon below as well as clone information before placing your order.

            References and tips

            Electronic file

            Remarks, protocol and/or map (gif) RDB03031.gif

            Original reference

            original Yogo, Y., Isolation of a possible archetypal JC virus DNA sequence from nonimmunocompromised individuals. J. Virol., 64, 3139-3143 (1990). PMID 2159570.

            Further references such as user reports and related articles (go to bottom)

            Featured content


            Sequence information

            RIKEN BRC has sequenced portions of this material for quality test.
            Please review the QC test results indicated by check icon as well as clone information before placing your order.

            Test sheet RDB17446_A9F3p1-1.pdf check

            Nucleotide sequence of a portion of this resource (if available).

            Primer: pUC-CAPbs_up_F
            Sequence file: RDB17446_A9F3a.seq check
            >03031_12319_A9F3_pUC-CAPbs_up_F_A04_01_ABI24.ab1
                1 CTCAGTGCAG CGGGCAGTGA GCGCACGCAA TTAATGTGAG TTAGCTCACT CATTAGGCAC
               61 CCCAGGCTTT ACACTTTATG CTTCCGGCTC GTATGTTGTG TGGAATTGTG AGCGGATAAC
              121 AATTTCACAC AGGAAACAGC TATGACCATG ATTACGCCAA GCTTGCATGC CTGCAGGTCG
              181 ACTCTAGAGG ATCCCAACAC TCTACCCCAC CTAAGAAGAA AAAAAAGGTA GAAGACCCTA
              241 AAGACTTTCC TGTAGATCTG CATGCATTCC TCAGTCAAGC TGTGTTTAGT AATAGAACTG
              301 TTGCTTCTTT TGCTGTGTAT ACCACTAAAG AAAAAGCTCA AATTTTATAT AAGAAACTTA
              361 TGGAAAAATA TTCTGTAACC TTTATAAGTA GACATGGTTT TGGGGGTCAT AATATTTTGT
              421 TTTTTTTAAC ACCACATAGA CATAGAGTTT CAGCAATTAA TAATTACTGT CAGAAACTAT
              481 GTACCTTTAG TTTTTTAATT TGTAAAGGTG TGAATAAGGA ATACTTGTTT TACAGTGCCT
              541 TGTGTAGACA GCCATATGCA GTAGTGGAAG AAAGTATTCA GGGGGGCCTT AAGGAGCATG
              601 ACTTTAACCC AGAAGAACCA GAAGAAACTA AGCAGGTTTC ATGGAAATTA GTTACACAGT
              661 ATGCTTTGGA CACCAAGTGT GAGGATGTTT TCTTGCTTAT GGGCATGTAC TTAAACTTTC
              721 AGGAAAACCC ACTGCAATGC AAAAAATGTG AAAAGAAGGA TCAGCCAAAT CACTTTAACC
              781 ATCATGAAAA GCACTATTAT AATGCCCAAA TTTTTGCAGA TAGCAAAAAT CAAAAAAGCA
              841 TTTGCCAGCA GGCTGTTGAT ACTGTAGCAG CCAAACAAAG GGTTGACAGT CTCCATATGA
              901 CCAGAGAAGA AATGTTAGTT GAAAGGTTTA ATTTTTTGCT TGATAAAATG GGACTTAATT
              961 TTTGGGGCAC ATGGCAATGC TGTTTTAGAG CATATATGGG CTGGGTGGGC CTGGATTCAT
             1021 TGTTGCTGCC TCAAATGACA CAGTCATTTA TGAGTTCTAA AATGCATTGT ATAAAACATC
             1081 AAAAAAAAGT ACTGGCTATC CAAGGCCATA GAACAGTTGC CAAAAACTAC TTTAACGCTG
            1141 GCAAGGCTGT TACC
            //
            Primer: pUC-M13_-100
            Sequence file: RDB17446_A9F3b.seq check
            >03031_12319_A9F3_pUC-M13_-100_B04_04_ABI24.ab1
                1 CGGCAGGGCG GAATTAAGTT GGGTACGCCA GGGTTTTCCC AGTCACGACG TTGTAAAACG
               61 ACGGCCAGTG AATTCGAGCT CGGTACCCGG GGATCCTGTG TTTTCATCAT CACTGGCAAA
              121 CATTTCTTCA TGGCAAAACA GGTCTTCATC CCACTTCTCA TTAAATGTAT TCCACCAGGA
              181 TTCCCACTCA TCTGTTCCAT AGGTTGGCAC CTAAAAACAA AAAATTCAGT TTATGTAAAA
              241 AACAAAATGC CCTGCAAAAG AAAAATTGTG GTTTACCTTA AAGCTTTAGA TCCCTGTAGG
              301 GGGTGTCTCC AAGAACCTTC TCCCAGCAAT GAAGAGCTTC TTGGGTTAAG TCACACCCAA
              361 ACCATTGTCT GAAGCAATCA AAGCAATAGC AATCTATCCA CACAAGTGGG CTGCTTCTTA
              421 AAAATTTTCT GTTTCTATGC CTTAATTTTA GCATGCACAT TAAACAGGGG CAATGCACTG
              481 AAGGATTAGT GGCACAATTA GGCCATTCCT TGCAATAAAG AGTATCAGAA TTAGGAGGAA
              541 AATCACAACC AACCTCTGAA CTATTCCATG TACCAAAATC AGGCTGATGA GCAACTTTTA
              601 CACCTTGTTC CATTTTTTTA TATAAAAAGT TCATTCTCTT CATTTTGTCT TCGTCCCCAC
              661 CTTTATCAGG GTGAAGTTCT TTGCATTTTT TCAGATAAGC TTTTCTCATG ACAGGAATGT
              721 TTCCCCATGC AGATCTATCA AGGCCTAATA AATCCATGAG CTCCATGGAT TCCTCCCTAT
              781 TCAGCACTTT GTCCATTTTT GCTTTTTGTA GCAAAAAATT AGTGCAAAAA AGGGAAAAAC
              841 AAGGGAATTT CCCTGGCCTC CTAAAAAGCC TCCACGCCCT TACTACTTCT GAGTAAGCTT
              901 GGAGGCGGAG GCGGCCTCGG CCTCCTGTAT ATATAAAAAA AAAGGGAAGG TAGGGGAGGA
              961 GCTGGCTAAA ACTGGGATGG GCTGCCAGCC AAGCATGAGC TCATACCCTA GGGAGCCAAC
             1021 CCAGCTGACA GCCCAGAGGG AGCCCTGCTG CAATGCCACT GTAGTTATAG TGGAAACCCT
             1081 CCATAGTTCT TTATTCACAG TAACAAGCAC AGGGAAGTTG AAAGCAGCCC AGGGGACAAT
            1141 GT
            //
            Primer: JCsp_f1
            Sequence file: RDB17446_A9F3c.seq check
            >03031_12319_A9F3_JCsp_f1_A01_01_ABI24.ab1
                1 TTCCTTGAGC ATATGAAGAT GGCCCCAACA AAAAGAAAAG GAGAAAGGAA GGACCCCGTG
               61 CAAGTTCCAA AACTTCTTAT AAGAGGAGGA GTAGAAGTTC TAGAAGTTAA AACTGGGGTT
              121 GACTCAATTA CAGAGGTAGA ATGTTTTTTA ACTCCAGAAA TGGGTGACCC AGATGAGCAT
              181 CTTAGGGGTT TTAGTAAGTC AATTTCTATA TCAGATACAT TTGAAAGTGA CTCCCCAAAT
              241 AAGGACATGC TTCCTTGTTA CAGTGTGGCC AGAATTCCAC TACCCAATCT AAATGAGGAT
              301 CTAACCTGTG GAAATATACT AATGTGGGAG GCTGTGACCT TAAAAACTGA GGTTATAGGG
              361 GTAACAACTT TGATGAATGT GCACTCTAAT GGTCAAGCAA CTCATGACAA TGGTGCAGGA
              421 AAGCCAGTGC AGGGCACCAG CTTTCATTTT TTTTCTGTTG GGGGGGAGGC TTTAGAATTA
              481 CAGGGGGTGG TTTTTAACTA CAGAACAAAG TACCCAGATG GAACAATTTT TCCAAAGAAT
              541 GCAACAGTGC AATCTCAAGT AATGAACACA GAGCACAAGG CGTACCTAGA TAAGAACAAA
              601 GCATATCCTG TTGAATGTTG GGTTCCTGAT CCCACCAGAA ATGAAAACAC AAGATATTTT
              661 GGGACACTAA CAGGAGGAGA AAATGTTCCT CCAGTTCTTC ATATAACAAA CACTGCCACA
              721 AACAGTGCTG CTTGATGAAT TTGGTGTTGG GCCCACTTTG CAAAGGTGAC AACTTGTATT
              781 TGTCAGCTGT GGATGTTTGT GGCATGTTTA CTAACAGATC TGGTTCCCAG CAGTGGAGAG
              841 GACTGTCCAG ATATTTTAAA GGTTCAGCTA AGAAAAAGGA GGGTTAAAAA CCCCCTAACC
              901 CAATTTCTTT CCTTTCTTAC TGATTTAATT AACAGAAGGA ACCCCTAAAT TTGATGGGGC
              961 AGCCTATGTA TGGGCATGGA ATGCCTCAGG TAGAGGGAGG GTTAGAGTTT TTTGTAGGGG
            1021 GAAA
            //
            Primer: JCsp_R2
            Sequence file: RDB17446_A9F3d.seq check
            >03031_12319_A9F3_JCsp_R2_A03_03_ABI24.ab1
                1 CGGCAAGTAA AGCTGGTTAT ACTTTATTAA AATGTACTGC ATATTACAAT AAAGGCTTTT
               61 GATTACAGCA TCTTTGTCTG CAACTGTCCA TATCTGTCAA CATATCTCAT CATGTCTGGG
              121 TCCCCTGGAA GTTCCTCTGT CCCCTCAAAA ACTCTAACCT CCTCTACCTG AGCATCCATG
              181 CCATACATAG GCTGCCCATC AACTCTAGGG GTCCTTCTGT TAATTAAATC AGTAAGAAGG
              241 AAAGAAATTG GGTAGGGGTT TTTAACCCTC CTTTTTCTTA GCTGAACCTT AAAATATCTG
              301 GACAGTCCTC TCCACTGCTG GGAACCAGAT CTGTTAGTAA ACATGCCACA AACATCCACA
              361 GCTGACAAAT ACAAGTTGTC ACCTTTGCAA AGTGGCCCAA CACCAAATTC ATCAAGCAGC
              421 ACTGTTGTGG CAGTGTTTGT TATATGAAGA ACTGGAGGAA CATTTTCTCC TCCTGTTAGT
              481 GTCCCAAAAT ATCTTGTGTT TTCATTTCTG GTGGGATCAG GAACCCAACA TTCAACAGGA
              541 TATGCTTTGT TCTTATCTAG GTACGCCTTG TGCTCTGTGT TCATTACTTG AGATTGCACT
              601 GTTGCATTCT TTGGAAAAAT TGTTCCATCT GGGTACTTTG TTCTGTAGTT AAAAACCACC
              661 CCCTGTAATT CTAAAGCCTC CCCCCCAACA GAAAAAAAAT GAAAGCTGGT GCCCTGCACT
              721 GGCTTTCCTG CACCATTGTC ATGAGTTGCT TGACCATTAG AGTGCACATT CATCAAAGTT
              781 GTTACCCCTA TAACCTCAGT TTTTAAGGTC ACAGCCTCCC ACATTAGTAT ATTTCCACAG
              841 GTTAGATCCT CATTTAGATT GGGTAGTGGA ATTCTGGCCA CACTGTAACA AGGAAGCATG
              901 TCCTTATTTG GGGAGTCACT TTCAAATGTA TCTGATATAG AAATTGACTT ACTAAAACCC
              961 CTAAGATGCT CATCTGGGTC ACCCATTTTC TGGAGTTAAA AAACATTTCT ACCTCTGTAT
             1021 TGAGTCACCC CAGTTTTTAC TCTAGACTCT ACTCCTTCTC TATAGAGTTT TGGAACTTGC
            1081 ACTGGTCATC CATTCTCGAT ACCGATCTGG AATGGGGGAT CATA
            //
            Primer: JCsp_f4
            Sequence file: RDB17446_A9F3e.seq check
            >03031_12319_A9F3_JCsp_f4_E01_13_ABI24.ab1
                1 ACATCATGGG ATTCTCCCTA TTCAGCACTT TGTCCATTTT TGCTTTTTGT AGCAAAAAAT
               61 TAGTGCAAAA AAGGGAAAAA CAAGGGAATT TCCCTGGCCT CCTAAAAAGC CTCCACGCCC
              121 TTACTACTTC TGAGTAAGCT TGGAGGCGGA GGCGGCCTCG GCCTCCTGTA TATATAAAAA
              181 AAAGGGAAGG TAGGGAGGAG CTGGCTAAAA CTGGATGGCT GCCAGCCAAG CATGAGCTCA
              241 TACCTAGGGA GCCAACCAGC TGACAGCCAG AGGGAGCCCT GGCTGCATGC CACTGGCAGT
              301 TATAGTGAAA CCCCTCCCAT AGTCCTTAAT CACAAGTAAA CAAAGCACAA GGGGAAGTGG
              361 AAAGCAGCCA GGGGAACATG TTTTGCGAGC CAGAGCTGTT TTGGCTTGTC ACCAGCTGGC
              421 CATGGTTCTT CGCCAGCTGT CACGTAAGGC TTCTGTGAAA GTTAGTAAAA CCTGGAGTGG
              481 AACTAAAAAA AGAGCTCAAA GGATTTTAAT TTTTTTGTTA GAATTTTTGC TGGACTTTTG
              541 CACAGGTGAA GACAGTGTAG ACGGGAAAAA AAGACAGAAA CACAGTGGTT TGACTGAGCA
              601 GAGATACAGT GCTTTGCCTG AACCAAAAGC TACATAGGTA AGTAATGTTT TTTTTTGTGT
              661 TTTCAGGTTC ATGGGTGCCG CACTTGCACT TTTGGGGGAC CTAGTTGCTA CTGTTTCTGA
              721 GGCTGCTGCT GCCACAGGAT TTTCAGTAGC TGAAATTGCT GCTGGAGAGG CTGCTGCTAC
              781 TATAGAAGTT GAAATTGCAT CCCTTGCTAC TGTAGAGGGG ATTACAAGTA CCTCTGAGGC
              841 TATAGCTGCT ATAGGCCTTA CTCCTGAAAC ATATGCTGTA ATTACTGGAG CTCCGGGGGG
              901 CTGTAGCTGG GTTTGCTGCA TTGGTTCAAA CTGTAACTGG TGGTAGTGCT ATTGCTCAGT
              961 TGGGATATAG ATTTTTTTGC TGACTGGGAT CATAAAGTTT CAACAGTTTG GGCTTTTTTC
             1021 AGCAGCAGCT ATGGCTTTAC AGTTATTTAA TCCCAGAGAC TACTATGATA TTTAATTTCT
             1081 TGGAGTGAAT GCTTTGATAC ATATTCACTA TTAGATTCTT AGAACATGGG GGGCCATTCG
            1141 TTTGAATCTT CGA
            //

            Please visit Sequencing and PCR primers for primer information.


            References

            Original, user report and related articles

            original Yogo, Y., Isolation of a possible archetypal JC virus DNA sequence from nonimmunocompromised individuals. J. Virol., 64, 3139-3143 (1990). PMID 2159570.

            2022.05.18

            GNP_filter3_RDBDEP_html_220516.pl