Resource data sheet
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pCY

Complete genome of JC virus, archetype

Catalog number RDB03031
Resource name pCY
Clone info. Complete genome of JC virus, archetype.
Known differences: A to C substitution (Gly, synonym) at 2446 nt of AB038249.1.
Vector backbone pUC19 (Plasmid)
Size of vector backbone 2,690 bp
Selectable markers Amp^r
Gene/insert name Hum_Polyoma JCV genome Genomic DNA
Reference of nucleotide sequence AB038249 (DDBJ accession number)
Depositor Yogo, Yoshiaki |

Distribution information

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Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature(s) designated by the DEPOSITOR is requested.
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RDB03031 pCY DNA solution

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References and tips

Electronic file

Electronic file Map RDB03031.gif from Depositor

Original reference

original Yogo, Y., Isolation of a possible archetypal JC virus DNA sequence from nonimmunocompromised individuals. J. Virol., 64, 3139-3143 (1990). PMID 2159570.

Further references such as user reports and related articles (go to bottom)

Featured content


Sequence information

RIKEN BRC has sequenced portions of this material for quality test. Primers and the results are shown below.

Data are summerized on test sheet below.

Test sheet RDB17446_A9F3p1-1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: pUC-CAPbs_up_F RDB17446_A9F3a.seq
>03031_12319_A9F3_pUC-CAPbs_up_F_A04_01_ABI24.ab1
    1 CTCAGTGCAG CGGGCAGTGA GCGCACGCAA TTAATGTGAG TTAGCTCACT CATTAGGCAC
   61 CCCAGGCTTT ACACTTTATG CTTCCGGCTC GTATGTTGTG TGGAATTGTG AGCGGATAAC
  121 AATTTCACAC AGGAAACAGC TATGACCATG ATTACGCCAA GCTTGCATGC CTGCAGGTCG
  181 ACTCTAGAGG ATCCCAACAC TCTACCCCAC CTAAGAAGAA AAAAAAGGTA GAAGACCCTA
  241 AAGACTTTCC TGTAGATCTG CATGCATTCC TCAGTCAAGC TGTGTTTAGT AATAGAACTG
  301 TTGCTTCTTT TGCTGTGTAT ACCACTAAAG AAAAAGCTCA AATTTTATAT AAGAAACTTA
  361 TGGAAAAATA TTCTGTAACC TTTATAAGTA GACATGGTTT TGGGGGTCAT AATATTTTGT
  421 TTTTTTTAAC ACCACATAGA CATAGAGTTT CAGCAATTAA TAATTACTGT CAGAAACTAT
  481 GTACCTTTAG TTTTTTAATT TGTAAAGGTG TGAATAAGGA ATACTTGTTT TACAGTGCCT
  541 TGTGTAGACA GCCATATGCA GTAGTGGAAG AAAGTATTCA GGGGGGCCTT AAGGAGCATG
  601 ACTTTAACCC AGAAGAACCA GAAGAAACTA AGCAGGTTTC ATGGAAATTA GTTACACAGT
  661 ATGCTTTGGA CACCAAGTGT GAGGATGTTT TCTTGCTTAT GGGCATGTAC TTAAACTTTC
  721 AGGAAAACCC ACTGCAATGC AAAAAATGTG AAAAGAAGGA TCAGCCAAAT CACTTTAACC
  781 ATCATGAAAA GCACTATTAT AATGCCCAAA TTTTTGCAGA TAGCAAAAAT CAAAAAAGCA
  841 TTTGCCAGCA GGCTGTTGAT ACTGTAGCAG CCAAACAAAG GGTTGACAGT CTCCATATGA
  901 CCAGAGAAGA AATGTTAGTT GAAAGGTTTA ATTTTTTGCT TGATAAAATG GGACTTAATT
  961 TTTGGGGCAC ATGGCAATGC TGTTTTAGAG CATATATGGG CTGGGTGGGC CTGGATTCAT
 1021 TGTTGCTGCC TCAAATGACA CAGTCATTTA TGAGTTCTAA AATGCATTGT ATAAAACATC
 1081 AAAAAAAAGT ACTGGCTATC CAAGGCCATA GAACAGTTGC CAAAAACTAC TTTAACGCTG
1141 GCAAGGCTGT TACC
//
Primer: pUC-M13_-100 RDB17446_A9F3b.seq
>03031_12319_A9F3_pUC-M13_-100_B04_04_ABI24.ab1
    1 CGGCAGGGCG GAATTAAGTT GGGTACGCCA GGGTTTTCCC AGTCACGACG TTGTAAAACG
   61 ACGGCCAGTG AATTCGAGCT CGGTACCCGG GGATCCTGTG TTTTCATCAT CACTGGCAAA
  121 CATTTCTTCA TGGCAAAACA GGTCTTCATC CCACTTCTCA TTAAATGTAT TCCACCAGGA
  181 TTCCCACTCA TCTGTTCCAT AGGTTGGCAC CTAAAAACAA AAAATTCAGT TTATGTAAAA
  241 AACAAAATGC CCTGCAAAAG AAAAATTGTG GTTTACCTTA AAGCTTTAGA TCCCTGTAGG
  301 GGGTGTCTCC AAGAACCTTC TCCCAGCAAT GAAGAGCTTC TTGGGTTAAG TCACACCCAA
  361 ACCATTGTCT GAAGCAATCA AAGCAATAGC AATCTATCCA CACAAGTGGG CTGCTTCTTA
  421 AAAATTTTCT GTTTCTATGC CTTAATTTTA GCATGCACAT TAAACAGGGG CAATGCACTG
  481 AAGGATTAGT GGCACAATTA GGCCATTCCT TGCAATAAAG AGTATCAGAA TTAGGAGGAA
  541 AATCACAACC AACCTCTGAA CTATTCCATG TACCAAAATC AGGCTGATGA GCAACTTTTA
  601 CACCTTGTTC CATTTTTTTA TATAAAAAGT TCATTCTCTT CATTTTGTCT TCGTCCCCAC
  661 CTTTATCAGG GTGAAGTTCT TTGCATTTTT TCAGATAAGC TTTTCTCATG ACAGGAATGT
  721 TTCCCCATGC AGATCTATCA AGGCCTAATA AATCCATGAG CTCCATGGAT TCCTCCCTAT
  781 TCAGCACTTT GTCCATTTTT GCTTTTTGTA GCAAAAAATT AGTGCAAAAA AGGGAAAAAC
  841 AAGGGAATTT CCCTGGCCTC CTAAAAAGCC TCCACGCCCT TACTACTTCT GAGTAAGCTT
  901 GGAGGCGGAG GCGGCCTCGG CCTCCTGTAT ATATAAAAAA AAAGGGAAGG TAGGGGAGGA
  961 GCTGGCTAAA ACTGGGATGG GCTGCCAGCC AAGCATGAGC TCATACCCTA GGGAGCCAAC
 1021 CCAGCTGACA GCCCAGAGGG AGCCCTGCTG CAATGCCACT GTAGTTATAG TGGAAACCCT
 1081 CCATAGTTCT TTATTCACAG TAACAAGCAC AGGGAAGTTG AAAGCAGCCC AGGGGACAAT
1141 GT
//
Primer: JCsp_f1 RDB17446_A9F3c.seq
>03031_12319_A9F3_JCsp_f1_A01_01_ABI24.ab1
    1 TTCCTTGAGC ATATGAAGAT GGCCCCAACA AAAAGAAAAG GAGAAAGGAA GGACCCCGTG
   61 CAAGTTCCAA AACTTCTTAT AAGAGGAGGA GTAGAAGTTC TAGAAGTTAA AACTGGGGTT
  121 GACTCAATTA CAGAGGTAGA ATGTTTTTTA ACTCCAGAAA TGGGTGACCC AGATGAGCAT
  181 CTTAGGGGTT TTAGTAAGTC AATTTCTATA TCAGATACAT TTGAAAGTGA CTCCCCAAAT
  241 AAGGACATGC TTCCTTGTTA CAGTGTGGCC AGAATTCCAC TACCCAATCT AAATGAGGAT
  301 CTAACCTGTG GAAATATACT AATGTGGGAG GCTGTGACCT TAAAAACTGA GGTTATAGGG
  361 GTAACAACTT TGATGAATGT GCACTCTAAT GGTCAAGCAA CTCATGACAA TGGTGCAGGA
  421 AAGCCAGTGC AGGGCACCAG CTTTCATTTT TTTTCTGTTG GGGGGGAGGC TTTAGAATTA
  481 CAGGGGGTGG TTTTTAACTA CAGAACAAAG TACCCAGATG GAACAATTTT TCCAAAGAAT
  541 GCAACAGTGC AATCTCAAGT AATGAACACA GAGCACAAGG CGTACCTAGA TAAGAACAAA
  601 GCATATCCTG TTGAATGTTG GGTTCCTGAT CCCACCAGAA ATGAAAACAC AAGATATTTT
  661 GGGACACTAA CAGGAGGAGA AAATGTTCCT CCAGTTCTTC ATATAACAAA CACTGCCACA
  721 AACAGTGCTG CTTGATGAAT TTGGTGTTGG GCCCACTTTG CAAAGGTGAC AACTTGTATT
  781 TGTCAGCTGT GGATGTTTGT GGCATGTTTA CTAACAGATC TGGTTCCCAG CAGTGGAGAG
  841 GACTGTCCAG ATATTTTAAA GGTTCAGCTA AGAAAAAGGA GGGTTAAAAA CCCCCTAACC
  901 CAATTTCTTT CCTTTCTTAC TGATTTAATT AACAGAAGGA ACCCCTAAAT TTGATGGGGC
  961 AGCCTATGTA TGGGCATGGA ATGCCTCAGG TAGAGGGAGG GTTAGAGTTT TTTGTAGGGG
1021 GAAA
//
Primer: JCsp_R2 RDB17446_A9F3d.seq
>03031_12319_A9F3_JCsp_R2_A03_03_ABI24.ab1
    1 CGGCAAGTAA AGCTGGTTAT ACTTTATTAA AATGTACTGC ATATTACAAT AAAGGCTTTT
   61 GATTACAGCA TCTTTGTCTG CAACTGTCCA TATCTGTCAA CATATCTCAT CATGTCTGGG
  121 TCCCCTGGAA GTTCCTCTGT CCCCTCAAAA ACTCTAACCT CCTCTACCTG AGCATCCATG
  181 CCATACATAG GCTGCCCATC AACTCTAGGG GTCCTTCTGT TAATTAAATC AGTAAGAAGG
  241 AAAGAAATTG GGTAGGGGTT TTTAACCCTC CTTTTTCTTA GCTGAACCTT AAAATATCTG
  301 GACAGTCCTC TCCACTGCTG GGAACCAGAT CTGTTAGTAA ACATGCCACA AACATCCACA
  361 GCTGACAAAT ACAAGTTGTC ACCTTTGCAA AGTGGCCCAA CACCAAATTC ATCAAGCAGC
  421 ACTGTTGTGG CAGTGTTTGT TATATGAAGA ACTGGAGGAA CATTTTCTCC TCCTGTTAGT
  481 GTCCCAAAAT ATCTTGTGTT TTCATTTCTG GTGGGATCAG GAACCCAACA TTCAACAGGA
  541 TATGCTTTGT TCTTATCTAG GTACGCCTTG TGCTCTGTGT TCATTACTTG AGATTGCACT
  601 GTTGCATTCT TTGGAAAAAT TGTTCCATCT GGGTACTTTG TTCTGTAGTT AAAAACCACC
  661 CCCTGTAATT CTAAAGCCTC CCCCCCAACA GAAAAAAAAT GAAAGCTGGT GCCCTGCACT
  721 GGCTTTCCTG CACCATTGTC ATGAGTTGCT TGACCATTAG AGTGCACATT CATCAAAGTT
  781 GTTACCCCTA TAACCTCAGT TTTTAAGGTC ACAGCCTCCC ACATTAGTAT ATTTCCACAG
  841 GTTAGATCCT CATTTAGATT GGGTAGTGGA ATTCTGGCCA CACTGTAACA AGGAAGCATG
  901 TCCTTATTTG GGGAGTCACT TTCAAATGTA TCTGATATAG AAATTGACTT ACTAAAACCC
  961 CTAAGATGCT CATCTGGGTC ACCCATTTTC TGGAGTTAAA AAACATTTCT ACCTCTGTAT
 1021 TGAGTCACCC CAGTTTTTAC TCTAGACTCT ACTCCTTCTC TATAGAGTTT TGGAACTTGC
1081 ACTGGTCATC CATTCTCGAT ACCGATCTGG AATGGGGGAT CATA
//
Primer: JCsp_f4 RDB17446_A9F3e.seq
>03031_12319_A9F3_JCsp_f4_E01_13_ABI24.ab1
    1 ACATCATGGG ATTCTCCCTA TTCAGCACTT TGTCCATTTT TGCTTTTTGT AGCAAAAAAT
   61 TAGTGCAAAA AAGGGAAAAA CAAGGGAATT TCCCTGGCCT CCTAAAAAGC CTCCACGCCC
  121 TTACTACTTC TGAGTAAGCT TGGAGGCGGA GGCGGCCTCG GCCTCCTGTA TATATAAAAA
  181 AAAGGGAAGG TAGGGAGGAG CTGGCTAAAA CTGGATGGCT GCCAGCCAAG CATGAGCTCA
  241 TACCTAGGGA GCCAACCAGC TGACAGCCAG AGGGAGCCCT GGCTGCATGC CACTGGCAGT
  301 TATAGTGAAA CCCCTCCCAT AGTCCTTAAT CACAAGTAAA CAAAGCACAA GGGGAAGTGG
  361 AAAGCAGCCA GGGGAACATG TTTTGCGAGC CAGAGCTGTT TTGGCTTGTC ACCAGCTGGC
  421 CATGGTTCTT CGCCAGCTGT CACGTAAGGC TTCTGTGAAA GTTAGTAAAA CCTGGAGTGG
  481 AACTAAAAAA AGAGCTCAAA GGATTTTAAT TTTTTTGTTA GAATTTTTGC TGGACTTTTG
  541 CACAGGTGAA GACAGTGTAG ACGGGAAAAA AAGACAGAAA CACAGTGGTT TGACTGAGCA
  601 GAGATACAGT GCTTTGCCTG AACCAAAAGC TACATAGGTA AGTAATGTTT TTTTTTGTGT
  661 TTTCAGGTTC ATGGGTGCCG CACTTGCACT TTTGGGGGAC CTAGTTGCTA CTGTTTCTGA
  721 GGCTGCTGCT GCCACAGGAT TTTCAGTAGC TGAAATTGCT GCTGGAGAGG CTGCTGCTAC
  781 TATAGAAGTT GAAATTGCAT CCCTTGCTAC TGTAGAGGGG ATTACAAGTA CCTCTGAGGC
  841 TATAGCTGCT ATAGGCCTTA CTCCTGAAAC ATATGCTGTA ATTACTGGAG CTCCGGGGGG
  901 CTGTAGCTGG GTTTGCTGCA TTGGTTCAAA CTGTAACTGG TGGTAGTGCT ATTGCTCAGT
  961 TGGGATATAG ATTTTTTTGC TGACTGGGAT CATAAAGTTT CAACAGTTTG GGCTTTTTTC
 1021 AGCAGCAGCT ATGGCTTTAC AGTTATTTAA TCCCAGAGAC TACTATGATA TTTAATTTCT
 1081 TGGAGTGAAT GCTTTGATAC ATATTCACTA TTAGATTCTT AGAACATGGG GGGCCATTCG
1141 TTTGAATCTT CGA
//

Please visit Sequencing and PCR primers for primer information.


References

Original, user report and related articles

original Yogo, Y., Isolation of a possible archetypal JC virus DNA sequence from nonimmunocompromised individuals. J. Virol., 64, 3139-3143 (1990). PMID 2159570.

2019.07.09

GNP_filter3_RDBDEP_html_190431.pl