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pDEST12.2-MAILL (#RDB03013)

Amammalian expression plasmid of mouse MAIL-L

Clone info. A mammalian expression plasmid of mouse MAIL-L. Primer sequence for RT-PCR is avairable. Expression was confirmed by depositor as follows: western blotting using a MALL-specific antibody.
Vector backbone pDEST12.2 (Plasmid)
Size of vector backbone 7,278 bp
Selectable markers Amp^r (E. coli), Neo^r (mammalian cell)
Gene/insert name Mouse MAIL (molecule possessing ankyrin-repeats induced by lipopolysaccharide) cDNA
Reference of insert sequence AB020974 (DDBJ accession number)
Depositor|Developer Kitamura, Hiroshi |
 

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RDB03013 pDEST12.2-MAILL DNA solution

Please review the QC test results indicated by check icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The pDEST12.2-MAILL was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB03013).

Reference section:

Kitamura, H., Kanehira, K., Okita, K., Morimatsu, M., Saito, M., MAIL, a novel nuclear I kappa B protein that potentiates LPS-induced IL-6 production. FEBS Lett., 485, 53-56 (2000). PMID 11086164. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB03013_02Ktp1-1.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: CMV_Forward (Pr0016)
Region: CMV pro,SP6 pro,attB1,insert 5'
Sequence file: RDB03013_02Kta.seq check
>03013_03013_B2EpF_CMV_Forward_A02_01_ABI08.ab1
    1 NNNNNNNNNN NNNNNNTGNN GCNNTGGGCG GTAGGCGTGT ACGGTGGGAG GTCTATATAA
   61 GCAGAGCTCG TTTAGTGAAC CGTCAGATCG CCTGGAGACG CCATCCACGC TGTTTTGACC
  121 TCCATAGAAG ACACCGGGAC CGATCCAGCC TCCGGACTCT AGCCTAGGCC GCGGGACGGA
  181 TAACAATTTC ACACAGGAAA CAGCTATGAC CATTAGGCCT TTGCAAAAAG CTATTTAGGT
  241 GACACTATAG AAGGTACGCC TGCAGGTACC GGATCACAAG TTTGTACAAA AAAGCAGGCT
  301 TAGCGCCTAG CCTGGGAGCA TGATCGTGGA CAAGCTGCTG GACGACAGCC GCGGCGGAGA
  361 GGGGCTGCTG GACGCGGCCG GCGACTGCGG CCTCATGACC AGCCCGCTCA ACCTGGCTTA
  421 CTTCTACGGC GCCTCGCCTC CTTCCGCCCC CGGCGCCGGC GACACCGGCT ACCTGTCCGC
  481 CGTGCCCTCC GCGCCCGGCT CGCCCGGCTC CGACTCCTCC GATTTCTCCT CCACTTCCTC
  541 CGTGTCCTCC TGCGGGGCCG TGGAGTCCCG TCCCAGAGGT GGCGCCCGCG CCGAGCGCCC
  601 TCAAGTTGAG CCCCACATGG GGGTTGGCAG GCAGCAGAGA GGCCCCTTTC AAGGTGTTCG
  661 CGTGAAGAAC TCGGTGAAGG AGCTCCTGCT ACACATCCGA AGCAACAAGC AGAAGGCTTC
  721 CGGCCAGCCC GTGGATGAGT TTAAGACACA GAGTGTGAAC ATTGAGCAGC TGACAGANCT
  781 GAAGAGTGCA GTGTCCGCAG TGGGGAAAAG AAAGGGACCC GATCCTCTGT CCNATGGACC
  841 GGTTTGCAAG ANGCCAGCTC TGTTGCCTTC TCACTTCGNG ACATCACCGC AAACGCCTAC
  901 ACCTGGGGAG AGCANGGAAN ANGNTCGCCA CAGCNAATCC NAGCTGGATA GCANCGCTGC
  961 CCNGCTTNNN AATANTATAA ANATCNNNAA TGANTGNNNC CNANTTTCNC TNAACNCNGN
1021 NCNNNNTANC TNNANGANCC CNNNNNNNNN CCNNNNNNNN NN
//
Primer: M13_-40 (Pr0410)
Region: T7 pro,attB2,insert 3'
Sequence file: RDB03013_02Ktb.seq check
>03013_03013_B2EpF_M13_-40_B02_02_ABI08.ab1
    1 NNNNNNNNNN NNNNNNNNNA NANGACTNNC TATAGGGAGA GAGCTATGAC GTCGCATGCA
   61 CGCGATCACC ACTTTGTACA AGAAAGCTGG GTCCTAGTAT GGTGGTGCTC GCTGCTGAAT
  121 GGACTTCCCC TTCAGGATAC GTCGGATCTG TTCTCCCACT GGGCCATCGG GGACCAAATG
  181 CACGGGCTGT TCATTCTCCA AGTTCCGAGT ACTGGGGTCA GCTCCCTTCC TCATCAACAG
  241 GCGGACTGCG TCCAACTGTG TCACCCGATA CTGCAGGCTG GCAGCAACAT GGAGGGCGGT
  301 GTTTCCATTG TAAGCCTTGG CATTCACGAA AGACAGGCAA CTGGGCAACT CCAAAAAGAG
  361 GCGAATGAGT TCCAGATTTG CTTCTTCCGC TGCCAAGTGC AGGGCTGTGC GGCCACTCTT
  421 ACGATCCTTT GCCTCCACTG CGGCTCCCAT CTGAATCAGA CACTTAATGG TGTCTACCAG
  481 GCTCTTATTC CTTAGTAAGA GGTCCTGCAC TTCGGGCGAA TGAGACTGCC GATTCCTCTG
  541 CAGTTCGTGC ACCACCGCAT TGTGAGCCAC GACCGCGCAA TGTAGGGGAG TCAGGCCATC
  601 ATAGTTCGTT GCCTCAAGAT CCACAAACTG ATTGCTCCTC ACAGCTCCCT TCTGGATGGC
  661 CTGGAGCACC TGGGAGTGGC CCTTCTCCGC ACAGACATGC AGAGGTGTCC TTCCCCAGCA
  721 ATCCGTGGTG TTCACCTGGG CACCAAGGTT TACCAGATCT TGCACAATGA GGTGCTGATT
  781 GGCAGCTACC GCCACCTGAA AGGCACTCTG TCCATTGTGC TCCTTAATGT CCAGCATGTG
  841 AAGAGCGTTC ATCTTTCTTG NANGGACATA CNAGANCGCT CGCCTNCCTT GGGCAACAGC
  901 AATATGGAGA AACGTGTCAN CATCTCCGNN CCTANCGAGG AANTGGTCCT GGGGAATATT
  961 TGCCAATTTG CTTTCTTNNN GNTCNACCTG NCNCTGAAAA AATNANNTNC CCANCTNGNN
1021 NNNATNCATN NGGNNTNNCN NNNNNNNNCT NNNNNNNNNN
//
Primer: SV40pro_ori_F (Pr0731)
Region: SV40 pro_ori,NeoR
Sequence file: RDB03013_02Ktc.seq check
>03013_03013_B2EpF_SV40pro_ori_F_C02_03_ABI08.ab1
    1 NNNNNNNNNN NNNNNNNNNN GCCTCNNCCT CTGAGCTATT CCNGAAGTAG TGAGGAGGCT
   61 TTTTTGGAGG CCTAGGCTTT TGCAAAAAGC TTGATTCTTC TGACACAACA GTCTCGAACT
  121 TAAGGCTAGA GCCACCATGA TTGAACAAGA TGGATTGCAC GCAGGTTCTC CGGCCGCTTG
  181 GGTGGAGAGG CTATTCGGCT ATGACTGGGC ACAACAGACA ATCGGCTGCT CTGATGCCGC
  241 CGTGTTCCGG CTGTCAGCGC AGGGGCGCCC GGTTCTTTTT GTCAAGACCG ACCTGTCCGG
  301 TGCCCTGAAT GAACTGCAGG ACGAGGCAGC GCGGCTATCG TGGCTGGCCA CGACGGGCGT
  361 TCCTTGCGCA GCTGTGCTCG ACGTTGTCAC TGAAGCGGGA AGGGACTGGC TGCTATTGGG
  421 CGAAGTGCCG GGGCAGGATC TCCTGTCATC TCACCTTGCT CCTGCCGAGA AAGTATCCAT
  481 CATGGCTGAT GCAATGCGGC GGCTGCATAC GCTTGATCCG GCTACCTGCC CATTCGACCA
  541 CCAAGCGAAA CATCGCATCG AGCGAGCACG TACTCGGATG GAAGCCGGTC TTGTCGATCA
  601 GGATGATCTG GACGAAGAGC ATCAGGGGCT CGCGCCAGCC GAACTGTTCG CCAGGCTCAA
  661 GGCGCGCATG CCCGACGGCG AGGATCTCGT CGTGACCCAT GGCGATGCCT GCTTGCCGAA
  721 TATCATGGTG GAAAATGGCC GCTTTTCTGG ATTCATCGAC TGTGGCCGGC TGGGTGTGGC
  781 GGANCGCTAT CANGNCATAG CGTTGGCTAC CCGTGATATT GCTGAAGAGC TTGGCGGCGA
  841 ATGGGCTGAC CGCTTCCTCG TGCTTTACNG TATCGCCGCT CCCNATTCGC AGCGCATCGC
  901 CTTCNATCNN NNTCTTGANN AGTTCNTCTG ANCGGGANTC TGGGGTTNGA AATGACNNAN
  961 CNAGCGANGC CCANNNNGNN NTCNCGANNN NCNNNNTAAA ANNNCTTTAT TTTCATTACN
1021 TCTGTGTGNT NGNNTTTTTN GNGTGNNNNN ANANNNNNNN NNN
//

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

original Kitamura, H., MAIL, a novel nuclear I kappa B protein that potentiates LPS-induced IL-6 production. FEBS Lett., 485, 53-56 (2000). PMID 11086164. [link to RRC of NBRP]