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pDEST12.2-MAILL

Amammalian expression plasmid of mouse MAIL-L

Catalog number RDB03013
Resource name pDEST12.2-MAILL
Clone info. Amammalian expression plasmid of mouse MAIL-L. Primer sequence for RT-PCR is avairable. Expression was confirmed by depositor as follows: western blotting using a MALL-specific antibody.
Vector backbone pDEST12.2 (Plasmid)
Size of vector backbone 7,278 bp
Selectable markers Amp^r (E. coli), Neo^r (mammalian cell)
Gene/insert name Mouse MAIL (molecule possessing ankyrin-repeats induced by lipopolysaccharide) cDNA
Reference of insert sequence AB020974 (DDBJ accession number)
Depositor|Developer Kitamura, Hiroshi |
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External Database
Mouse MAIL (molecule possessing ankyrin-repeats induced by lipopolysaccharide)

          Reference sequence
            

          Distribution information

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          RDB03013 pDEST12.2-MAILL DNA solution Please contact us.

          Please review the QC test results indicated by check icon below as well as clone information before placing your order.

          References and tips

          Electronic file

          Original reference

          original Kitamura, H., MAIL, a novel nuclear I kappa B protein that potentiates LPS-induced IL-6 production. FEBS Lett., 485, 53-56 (2000). PMID 11086164.

          Further references such as user reports and related articles (go to bottom)

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          Sequence information

          RIKEN BRC has sequenced portions of this material for quality test.
          Please review the QC test results indicated by check icon as well as clone information before placing your order.

          Test sheet RDB03013_02Ktp1-1.pdf check

          Nucleotide sequence of a portion of this resource (if available).

          Primer: CMV_Forward
          Region: CMV pro,SP6 pro,attB1,insert 5'
          Sequence file: RDB03013_02Kta.seq check
          >03013_03013_B2EpF_CMV_Forward_A02_01_ABI08.ab1
              1 NNNNNNNNNN NNNNNNTGNN GCNNTGGGCG GTAGGCGTGT ACGGTGGGAG GTCTATATAA
             61 GCAGAGCTCG TTTAGTGAAC CGTCAGATCG CCTGGAGACG CCATCCACGC TGTTTTGACC
            121 TCCATAGAAG ACACCGGGAC CGATCCAGCC TCCGGACTCT AGCCTAGGCC GCGGGACGGA
            181 TAACAATTTC ACACAGGAAA CAGCTATGAC CATTAGGCCT TTGCAAAAAG CTATTTAGGT
            241 GACACTATAG AAGGTACGCC TGCAGGTACC GGATCACAAG TTTGTACAAA AAAGCAGGCT
            301 TAGCGCCTAG CCTGGGAGCA TGATCGTGGA CAAGCTGCTG GACGACAGCC GCGGCGGAGA
            361 GGGGCTGCTG GACGCGGCCG GCGACTGCGG CCTCATGACC AGCCCGCTCA ACCTGGCTTA
            421 CTTCTACGGC GCCTCGCCTC CTTCCGCCCC CGGCGCCGGC GACACCGGCT ACCTGTCCGC
            481 CGTGCCCTCC GCGCCCGGCT CGCCCGGCTC CGACTCCTCC GATTTCTCCT CCACTTCCTC
            541 CGTGTCCTCC TGCGGGGCCG TGGAGTCCCG TCCCAGAGGT GGCGCCCGCG CCGAGCGCCC
            601 TCAAGTTGAG CCCCACATGG GGGTTGGCAG GCAGCAGAGA GGCCCCTTTC AAGGTGTTCG
            661 CGTGAAGAAC TCGGTGAAGG AGCTCCTGCT ACACATCCGA AGCAACAAGC AGAAGGCTTC
            721 CGGCCAGCCC GTGGATGAGT TTAAGACACA GAGTGTGAAC ATTGAGCAGC TGACAGANCT
            781 GAAGAGTGCA GTGTCCGCAG TGGGGAAAAG AAAGGGACCC GATCCTCTGT CCNATGGACC
            841 GGTTTGCAAG ANGCCAGCTC TGTTGCCTTC TCACTTCGNG ACATCACCGC AAACGCCTAC
            901 ACCTGGGGAG AGCANGGAAN ANGNTCGCCA CAGCNAATCC NAGCTGGATA GCANCGCTGC
            961 CCNGCTTNNN AATANTATAA ANATCNNNAA TGANTGNNNC CNANTTTCNC TNAACNCNGN
          1021 NCNNNNTANC TNNANGANCC CNNNNNNNNN CCNNNNNNNN NN
          //
          Primer: M13_-40
          Region: T7 pro,attB2,insert 3'
          Sequence file: RDB03013_02Ktb.seq check
          >03013_03013_B2EpF_M13_-40_B02_02_ABI08.ab1
              1 NNNNNNNNNN NNNNNNNNNA NANGACTNNC TATAGGGAGA GAGCTATGAC GTCGCATGCA
             61 CGCGATCACC ACTTTGTACA AGAAAGCTGG GTCCTAGTAT GGTGGTGCTC GCTGCTGAAT
            121 GGACTTCCCC TTCAGGATAC GTCGGATCTG TTCTCCCACT GGGCCATCGG GGACCAAATG
            181 CACGGGCTGT TCATTCTCCA AGTTCCGAGT ACTGGGGTCA GCTCCCTTCC TCATCAACAG
            241 GCGGACTGCG TCCAACTGTG TCACCCGATA CTGCAGGCTG GCAGCAACAT GGAGGGCGGT
            301 GTTTCCATTG TAAGCCTTGG CATTCACGAA AGACAGGCAA CTGGGCAACT CCAAAAAGAG
            361 GCGAATGAGT TCCAGATTTG CTTCTTCCGC TGCCAAGTGC AGGGCTGTGC GGCCACTCTT
            421 ACGATCCTTT GCCTCCACTG CGGCTCCCAT CTGAATCAGA CACTTAATGG TGTCTACCAG
            481 GCTCTTATTC CTTAGTAAGA GGTCCTGCAC TTCGGGCGAA TGAGACTGCC GATTCCTCTG
            541 CAGTTCGTGC ACCACCGCAT TGTGAGCCAC GACCGCGCAA TGTAGGGGAG TCAGGCCATC
            601 ATAGTTCGTT GCCTCAAGAT CCACAAACTG ATTGCTCCTC ACAGCTCCCT TCTGGATGGC
            661 CTGGAGCACC TGGGAGTGGC CCTTCTCCGC ACAGACATGC AGAGGTGTCC TTCCCCAGCA
            721 ATCCGTGGTG TTCACCTGGG CACCAAGGTT TACCAGATCT TGCACAATGA GGTGCTGATT
            781 GGCAGCTACC GCCACCTGAA AGGCACTCTG TCCATTGTGC TCCTTAATGT CCAGCATGTG
            841 AAGAGCGTTC ATCTTTCTTG NANGGACATA CNAGANCGCT CGCCTNCCTT GGGCAACAGC
            901 AATATGGAGA AACGTGTCAN CATCTCCGNN CCTANCGAGG AANTGGTCCT GGGGAATATT
            961 TGCCAATTTG CTTTCTTNNN GNTCNACCTG NCNCTGAAAA AATNANNTNC CCANCTNGNN
          1021 NNNATNCATN NGGNNTNNCN NNNNNNNNCT NNNNNNNNNN
          //
          Primer: SV40pro_ori_F
          Region: SV40 pro_ori,NeoR
          Sequence file: RDB03013_02Ktc.seq check
          >03013_03013_B2EpF_SV40pro_ori_F_C02_03_ABI08.ab1
              1 NNNNNNNNNN NNNNNNNNNN GCCTCNNCCT CTGAGCTATT CCNGAAGTAG TGAGGAGGCT
             61 TTTTTGGAGG CCTAGGCTTT TGCAAAAAGC TTGATTCTTC TGACACAACA GTCTCGAACT
            121 TAAGGCTAGA GCCACCATGA TTGAACAAGA TGGATTGCAC GCAGGTTCTC CGGCCGCTTG
            181 GGTGGAGAGG CTATTCGGCT ATGACTGGGC ACAACAGACA ATCGGCTGCT CTGATGCCGC
            241 CGTGTTCCGG CTGTCAGCGC AGGGGCGCCC GGTTCTTTTT GTCAAGACCG ACCTGTCCGG
            301 TGCCCTGAAT GAACTGCAGG ACGAGGCAGC GCGGCTATCG TGGCTGGCCA CGACGGGCGT
            361 TCCTTGCGCA GCTGTGCTCG ACGTTGTCAC TGAAGCGGGA AGGGACTGGC TGCTATTGGG
            421 CGAAGTGCCG GGGCAGGATC TCCTGTCATC TCACCTTGCT CCTGCCGAGA AAGTATCCAT
            481 CATGGCTGAT GCAATGCGGC GGCTGCATAC GCTTGATCCG GCTACCTGCC CATTCGACCA
            541 CCAAGCGAAA CATCGCATCG AGCGAGCACG TACTCGGATG GAAGCCGGTC TTGTCGATCA
            601 GGATGATCTG GACGAAGAGC ATCAGGGGCT CGCGCCAGCC GAACTGTTCG CCAGGCTCAA
            661 GGCGCGCATG CCCGACGGCG AGGATCTCGT CGTGACCCAT GGCGATGCCT GCTTGCCGAA
            721 TATCATGGTG GAAAATGGCC GCTTTTCTGG ATTCATCGAC TGTGGCCGGC TGGGTGTGGC
            781 GGANCGCTAT CANGNCATAG CGTTGGCTAC CCGTGATATT GCTGAAGAGC TTGGCGGCGA
            841 ATGGGCTGAC CGCTTCCTCG TGCTTTACNG TATCGCCGCT CCCNATTCGC AGCGCATCGC
            901 CTTCNATCNN NNTCTTGANN AGTTCNTCTG ANCGGGANTC TGGGGTTNGA AATGACNNAN
            961 CNAGCGANGC CCANNNNGNN NTCNCGANNN NCNNNNTAAA ANNNCTTTAT TTTCATTACN
          1021 TCTGTGTGNT NGNNTTTTTN GNGTGNNNNN ANANNNNNNN NNN
          //

          Please visit Sequencing and PCR primers for primer information.


          References

          Original, user report and related articles

          original Kitamura, H., MAIL, a novel nuclear I kappa B protein that potentiates LPS-induced IL-6 production. FEBS Lett., 485, 53-56 (2000). PMID 11086164.

          2022.06.16

          GNP_filter3_RDBDEP_html_220520.pl