Resource data sheet
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pCAEGFP

Expression vector of jellyfish GFP

Catalog number RDB03006
Resource name pCAEGFP
Clone info. Expression vector of jellyfish GFP. EGFP is regulated by CAG promoter.
Vector backbone pCAcc (Plasmid)
Size of vector backbone 4.83 kb
Selectable markers Amp^r
Gene/insert name Aequorea victoria GFP cDNA
Depositor Hamada, Hirofumi |

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Terms and conditions set forth by the DEPOSITOR The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit institution for a not-for-profit research. RECIPIENT must contact the DEPOSITOR in the case of application for any patents or commercial use based on the results from the use of the BIOLOGICAL RESOURCE. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested.
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Material Transfer Agreement (MTA) [Word]
Schedule A (GFP-A) [Link]
Remarks ((Additional form)) GFP-A
Please use Schedule A (GFP-A) additionally.
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Catalog # Resource name Shipping form Fee (non-profit org.)
RDB03006 pCAEGFP DNA solution

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Sequence information

RIKEN BRC has sequenced portions of this material for quality test. Primers and the results are shown below.

Data are summerized on test sheet below.

Test sheet RDB15919_A8E7p1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: EGFP-N RDB15919_A8E7a.seq
>03006_15919_A8E7_EGFP-N_E01_05_ABI08.ab1
    1 GCACCCCGGG GTGACGCTCC TCGCCCTTGC TCACCATGGT GGCGACCGGT GGATCCCGGG
   61 CCCGCGGTAC CGTCGACTGC AGAATTCTTT GCCAAAATGA TGAGACAGCA CAATAACCAG
  121 CACGTTGCCC AGGAGCTGTA GGAAAAAGAA GAAGGCATGA ACATGGTTAG CAGAGGCTCT
  181 AGAGCCGCCG GTCACACGCC AGAAGCCGAA CCCCGCCCTG CCCCGTCCCC CCCGAAGGCA
  241 GCCGTCCCCC CGCGGACAGC CCCGAGGCTG GAGAGGGAGA AGGGGACGGC GGCGCGGCGA
  301 CGCACGAAGG CCCTCCCCGC CCATTTCCTT CCTGCCGGCG CCGCACCGCT TCGCCCCGCG
  361 CCCGCTAGAG GGGGTGCGGC GGCGCCTCCC AGATTTCGGC TCCGCACAGA TTTGGGACAA
  421 AGGAAGTCCC TGCGCCCTCT CGCACGATTA CCATAAAAGG CAATGGCTGC GGCTCGCCGC
  481 GCCTCGACAG CCGCCGGCGC TCCGGGGGCC GCCGCGCCCC TCCCCCGAGC CCTCCCCGGC
  541 CCGAGGCGGC CCCGCCCCGC CCGGCACCCC CACCTGCCGC CACCCCCCGC CCGGCACGGC
  601 GAGCCCCGCG CCACGCCCCG TACGGAGCCC CGCACCCCGA AGCCGGGCCG TGCTCAGCAA
661 CTCGGGGAGG GGGGTG
//
Primer: pAxCA_F1 RDB15919_A8E7b.seq
>03006_15919_A8E7_pAxCA_F1_A01_01_ABI08.ab1
    1 CAGGACCCTG TGCTACATTG TTCATGCCTT CTTCTTTTTC CTACAGCTCC TGGGCAACGT
   61 GCTGGTTATT GTGCTGTCTC ATCATTTTGG CAAAGAATTC TGCAGTCGAC GGTACCGCGG
  121 GCCCGGGATC CACCGGTCGC CACCATGGTG AGCAAGGGCG AGGAGCTGTT CACCGGGGTG
  181 GTGCCCATCC TGGTCGAGCT GGACGGCGAC GTAAACGGCC ACAAGTTCAG CGTGTCCGGC
  241 GAGGGCGAGG GCGATGCCAC CTACGGCAAG CTGACCCTGA AGTTCATCTG CACCACCGGC
  301 AAGCTGCCCG TGCCCTGGCC CACCCTCGTG ACCACCCTGA CCTACGGCGT GCAGTGCTTC
  361 AGCCGCTACC CCGACCACAT GAAGCAGCAC GACTTCTTCA AGTCCGCCAT GCCCGAAGGC
  421 TACGTCCAGG AGCGCACCAT CTTCTTCAAG GACGACGGCA ACTACAAGAC CCGCGCCGAG
  481 GTGAAGTTCG AGGGCGACAC CCTGGTGAAC CGCATCGAGC TGAAGGGCAT CGACTTCAAG
  541 GAGGACGGCA ACATCCTGGG GCACAAGCTG GAGTACAACT ACAACAGCCA CAACGTCTAT
  601 ATCATGGCCG ACAAGCAGAA GAACGGCATC AAGGTGAACT TCAAGATCCG CCACAACATC
  661 GAGGACGGCA GCGTGCAGCT CGCCGACCAC TACCAGCAGA ACACCCCCAT CGGCGACGGC
  721 CCCGTGCTGC TGCCCGACAA CCACTACCTG AGCACCCAGT CCGCCCTGAG CAAAGACCCC
  781 AACGAGAAGC GCGATCACAT GGTCCTGCTG GAGTTCGTGA CCGCCGCCGG GATCACTCTC
  841 GGCATGGACG AGCTGTACAA GTAAAGCGGC CGATCTTTTT CCCTCTGCCA AAAATTATGG
  901 GGACATCATG AAGCCCCTTG AGCATCTGAC TTCTGGCTAA TAAAGGAAAT TTATTTTCAT
  961 TGCAATAGTG TGTTGGGAAT TTTTTTGTGT CTCTCACTCG GAAGGACATA TGGGGAGGGG
 1021 CAAATCATTT AAAACATCAG AATGAGTATT TGGGTTAGAG GTTTGGCAAC ATATGCCATA
 1081 TGCTGGCTGC CATGGAACAA AGGTTGGCCT TATAAGAGTT CATTCAGGTA TATGAAACCA
1141 GCTCCCTGCC TGGTCCAATT TCCTATTTTT CCATTAAGGA A
//

Please visit Sequencing and PCR primers for primer information.


References

References


2018.12.10

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