Resource data sheet
Prev.
Please review the QC test results indicated by check icon below as well as clone information before placing your order.

pCAEGFP

Expression vector of jellyfish GFP

Catalog number RDB03006
Resource name pCAEGFP
Clone info. Expression vector of jellyfish GFP. EGFP is regulated by CAG promoter.
Vector backbone pCAcc (Plasmid)
Size of vector backbone 4.83 kb
Selectable markers Amp^r
Gene/insert name Aequorea victoria GFP cDNA
Depositor|Developer Hamada, Hirofumi |
Other clones in our bank

External Database
Aequorea victoria GFP

            Reference sequence
              

            Distribution information

            Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
            Terms and conditions set forth by the DEPOSITOR The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit institution for a not-for-profit research. RECIPIENT must contact the DEPOSITOR in the case of application for any patents or commercial use based on the results from the use of the BIOLOGICAL RESOURCE. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested.
            Additional terms and conditions for distribution Regarding resources containing CAG promoter: In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation for the CAG promoter (Niwa, H., Yamamura, K., Miyazaki, J., Gene 108 : 193-200, 1991) and an acknowledgment to Dr. Jun-ich Miyazaki of the Osaka University are requested.
            Ordering Please visit Information of Request for Distribution.[link] 
            Order form 
            [Credit Card Payment]  [Bank Transfer Payment]
            Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word]
            Schedule A (GFP-A) [Link]
            Remarks
            Please use Schedule A (GFP-A) additionally.
            提供案内 (日本国内) [open/close]

            提供条件 非営利機関の非営利学術研究へ提供する。利用者が本件リソースを利用した研究成果に基づき特許申請、商業活動等を行う場合は、利用者は事前に寄託者に連絡する。利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する。利用者は、研究成果の公表にあたって謝辞の表明を必要とする。
            付加的提供条件 CAGプロモータを含むリソースについて: 利用者は、研究成果の公表にあたってCAGプロモータの文献 (Niwa, H., Yamamura, K., Miyazaki, J., Gene 108 : 193-200, 1991)を引用し、大阪大学 宮崎純一博士への謝辞の表明を必要とする。
            提供依頼 手続きの詳細は、「提供申込みについて[link]」をご覧ください。
            提供依頼書 [Word]
            提供同意書 (MTA、非営利機関による非営利学術研究用)[Word]
            Schedule A(GFP-A, 英語版のみ) [Link]
            備考
            Schedule A(GFP-A, 英語版のみ)が追加で必要です。

            Catalog # Resource name Shipping form Fee (non-profit org.)
            RDB03006 pCAEGFP DNA solution

            Ordering Information [in Japanese] [in English]
            Please review the QC test results indicated by check icon below as well as clone information before placing your order.

            References and tips

            Electronic file

            Original reference

            Further references such as user reports and related articles (go to bottom)

            Featured content

            Featured content Empty Backbone (English text)
            Featured content Empty Backbone (Japanese text)

            Sequence information

            RIKEN BRC has sequenced portions of this material for quality test.
            Please review the QC test results indicated by check icon as well as clone information before placing your order.

            Test sheet RDB15919_A8E7p1.pdf check

            Nucleotide sequence of a portion of this resource (if available).

            Primer: EGFP-N
            Sequence file: RDB15919_A8E7a.seq check
            >03006_15919_A8E7_EGFP-N_E01_05_ABI08.ab1
                1 GCACCCCGGG GTGACGCTCC TCGCCCTTGC TCACCATGGT GGCGACCGGT GGATCCCGGG
               61 CCCGCGGTAC CGTCGACTGC AGAATTCTTT GCCAAAATGA TGAGACAGCA CAATAACCAG
              121 CACGTTGCCC AGGAGCTGTA GGAAAAAGAA GAAGGCATGA ACATGGTTAG CAGAGGCTCT
              181 AGAGCCGCCG GTCACACGCC AGAAGCCGAA CCCCGCCCTG CCCCGTCCCC CCCGAAGGCA
              241 GCCGTCCCCC CGCGGACAGC CCCGAGGCTG GAGAGGGAGA AGGGGACGGC GGCGCGGCGA
              301 CGCACGAAGG CCCTCCCCGC CCATTTCCTT CCTGCCGGCG CCGCACCGCT TCGCCCCGCG
              361 CCCGCTAGAG GGGGTGCGGC GGCGCCTCCC AGATTTCGGC TCCGCACAGA TTTGGGACAA
              421 AGGAAGTCCC TGCGCCCTCT CGCACGATTA CCATAAAAGG CAATGGCTGC GGCTCGCCGC
              481 GCCTCGACAG CCGCCGGCGC TCCGGGGGCC GCCGCGCCCC TCCCCCGAGC CCTCCCCGGC
              541 CCGAGGCGGC CCCGCCCCGC CCGGCACCCC CACCTGCCGC CACCCCCCGC CCGGCACGGC
              601 GAGCCCCGCG CCACGCCCCG TACGGAGCCC CGCACCCCGA AGCCGGGCCG TGCTCAGCAA
            661 CTCGGGGAGG GGGGTG
            //
            Primer: pAxCA_F1
            Sequence file: RDB15919_A8E7b.seq check
            >03006_15919_A8E7_pAxCA_F1_A01_01_ABI08.ab1
                1 CAGGACCCTG TGCTACATTG TTCATGCCTT CTTCTTTTTC CTACAGCTCC TGGGCAACGT
               61 GCTGGTTATT GTGCTGTCTC ATCATTTTGG CAAAGAATTC TGCAGTCGAC GGTACCGCGG
              121 GCCCGGGATC CACCGGTCGC CACCATGGTG AGCAAGGGCG AGGAGCTGTT CACCGGGGTG
              181 GTGCCCATCC TGGTCGAGCT GGACGGCGAC GTAAACGGCC ACAAGTTCAG CGTGTCCGGC
              241 GAGGGCGAGG GCGATGCCAC CTACGGCAAG CTGACCCTGA AGTTCATCTG CACCACCGGC
              301 AAGCTGCCCG TGCCCTGGCC CACCCTCGTG ACCACCCTGA CCTACGGCGT GCAGTGCTTC
              361 AGCCGCTACC CCGACCACAT GAAGCAGCAC GACTTCTTCA AGTCCGCCAT GCCCGAAGGC
              421 TACGTCCAGG AGCGCACCAT CTTCTTCAAG GACGACGGCA ACTACAAGAC CCGCGCCGAG
              481 GTGAAGTTCG AGGGCGACAC CCTGGTGAAC CGCATCGAGC TGAAGGGCAT CGACTTCAAG
              541 GAGGACGGCA ACATCCTGGG GCACAAGCTG GAGTACAACT ACAACAGCCA CAACGTCTAT
              601 ATCATGGCCG ACAAGCAGAA GAACGGCATC AAGGTGAACT TCAAGATCCG CCACAACATC
              661 GAGGACGGCA GCGTGCAGCT CGCCGACCAC TACCAGCAGA ACACCCCCAT CGGCGACGGC
              721 CCCGTGCTGC TGCCCGACAA CCACTACCTG AGCACCCAGT CCGCCCTGAG CAAAGACCCC
              781 AACGAGAAGC GCGATCACAT GGTCCTGCTG GAGTTCGTGA CCGCCGCCGG GATCACTCTC
              841 GGCATGGACG AGCTGTACAA GTAAAGCGGC CGATCTTTTT CCCTCTGCCA AAAATTATGG
              901 GGACATCATG AAGCCCCTTG AGCATCTGAC TTCTGGCTAA TAAAGGAAAT TTATTTTCAT
              961 TGCAATAGTG TGTTGGGAAT TTTTTTGTGT CTCTCACTCG GAAGGACATA TGGGGAGGGG
             1021 CAAATCATTT AAAACATCAG AATGAGTATT TGGGTTAGAG GTTTGGCAAC ATATGCCATA
             1081 TGCTGGCTGC CATGGAACAA AGGTTGGCCT TATAAGAGTT CATTCAGGTA TATGAAACCA
            1141 GCTCCCTGCC TGGTCCAATT TCCTATTTTT CCATTAAGGA A
            //

            Please visit Sequencing and PCR primers for primer information.


            References

            Original, user report and related articles


            2022.02.09

            GNP_filter3_RDBDEP_html_220112.pl