RDB02977 - pMFGhB7-1 (CD80)

Please review the QC test results indicated by check

icon below as well as clone information before placing your order.

pMFGhB7-1 (CD80) (#RDB02977)

Retroviral vector of human B7-1 (CD80)

Alternative name	CD80
Clone info.	Retroviral vector of human B7-1 (CD80).
Vector backbone	pMFG (Plasmid)
Selectable markers	Amp^r
Gene/insert name	Human CD80 (B7-1) cDNA
Depositor\|Developer	Hamada, Hirofumi \|

Sequence	RDB02977z.seq

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.

Ordering forms
Order form [Credit Card Payment] [Bank Transfer Payment] [Example of order form ]
MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ]
Please visit Information of Request for Distribution.[link]

Terms and conditions for distribution The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit institution for a not-for-profit research. RECIPIENT must contact the DEPOSITOR in the case of application for any patents or commercial use based on the results from the use of the BIOLOGICAL RESOURCE. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the literature designated by the DEPOSITOR (J. Leukoc. Biol., 60, 181-190 (1996)) and an acknowledgment to the DEPOSITOR are requested.

Remarks Remember that you will be working with samples containing infectious virus.

提供案内 (日本国内) [open/close]

Ordering forms	Order form [Credit Card Payment] [Bank Transfer Payment] [Example of order form ] MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ] Please visit Information of Request for Distribution.[link]
Terms and conditions for distribution	The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit institution for a not-for-profit research. RECIPIENT must contact the DEPOSITOR in the case of application for any patents or commercial use based on the results from the use of the BIOLOGICAL RESOURCE. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the literature designated by the DEPOSITOR (J. Leukoc. Biol., 60, 181-190 (1996)) and an acknowledgment to the DEPOSITOR are requested.
Remarks	Remember that you will be working with samples containing infectious virus.

必要書類	提供依頼書 [依頼書の記入例 ] 提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ] 手続きの概要は、「提供申込みについて[link]」をご覧ください。
MTAに書く使用条件	非営利機関の非営利学術研究へ提供する。利用者が本件リソースを利用した研究成果に基づき特許申請、商業活動等を行う場合は、利用者は事前に寄託者に連絡する。利用者は、研究成果の公表にあたって寄託者の指定する文献 (J. Leukoc. Biol., 60, 181-190 (1996))を引用し、謝辞の表明を必要とする。
備考	このリソースはウイルス粒子産生用のため、取扱いに注意が必要です。

Catalog #	Resource name	Shipping form	Fee (non-profit org.)
RDB02977	pMFGhB7-1 (CD80)	DNA solution	JPY 9,460 (not-for-profit academic purpose) plus cost of shipping containers, dry ice (if required) and shipping charge

Please review the QC test results indicated by check

icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The pMFGhB7-1 (CD80) was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB02977).

Reference section:

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet	RDB15968_A8F6p1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: pMFG_F Sequence file: RDB15968_A8F6a.seq
>02977_D5679_A8F6_1_pMFG_F_A07_01_ABI24.ab1 1 TCTAAAATGG GGGGGCACCC CGCCCTTGTA ACTTCCCTGA CCCTGACATG ACAAGAGGTT 61 ACTAACAGCC CCTCTCTCCA AGCTCACTTA CAGGCTCTCT ACTTAGTCCA GCACGAAGTC 121 TGGAGACCTC TGGCGGCAGC CTACCAAGAA CAACTGGACC GACCGGTGGT ACCTCACCCT 181 TACCGAGTCG GCGACACAGT GTGGGTCCGC CGACACCAGA CTAAGAACCT AGAACCTCGC 241 TGGAAAGGAC CTTACACAGT CCTGCTGACC ACCCCCACCG CCCTCAAAGT AGACGGCATC 301 GCAGCTTGGA TACACGCCGC CCACGTGAAG GCTGCCGACC CCGGGGGTGG ACCATCCTCT 361 AGAACACCAT GGGCCACACA CGGAGGCAGG GAACATCACC ATCCAAGTGT CCATACCTCA 421 ATTTCTTTCA GCTCTTGGTG CTGGCTGGTC TTTCTCACTT CTGTTCAGGT GTTATCCACG 481 TGACCAAGGA AGTGAAAGAA GTGGCAACGC TGTCCTGTGG TCACAATGTT TCTGTTGAAG 541 AGCTGGCACA AACTCGCATC TACTGGCAAA AGGAGAAGAA AATGGTGCTG ACTATGATGT 601 CTGGGGACAT GAATATATGG CCCGAGTACA AGAACCGGAC CATCTTTGAT ATCACTAATA 661 ACCTCTCCAT TGTGATCCTG GCTCTGCGCC CATCTGACGA GGGCACATAC GAGTGTGTTG 721 TTCTGAAGTA TGAAAAAGAC GCTTTCAAGC GGGAACACCT GGCTGAAGTG ACGTTATCAG 781 TCAAAGCTGA CTTCCCTACA CCTAGTATAT CTGACTTTGA AATTCCAACT TCTAATATTA 841 GAAGGATAAT TTGCTCAACC TCTGGAGGTT TTCCAGAGCC TCACCTCTCC TGGTTGGAAA 901 ATGGAGAAGA ATTAAATGCC ATCAACACAA CAGTTTCCCA AGATCCTGAA ACTGAGCTCT 961 ATGCTGTTAG CAGCAAACTG GATTTCAATA TGACAACCAA CCACAGCCTT CATGTGTTCT 1021 TCATTCAAGG TAATGGACAT TTTAAGAGGT GAACTCAGAC CTTCCTACCT GAATACCATC 1081 CAGGCAAGAC ATCT //
Primer: pMXs-3LTRup_R2 Sequence file: RDB15968_A8F6b.seq
>02977_D5679_A8F6_1_pMXs-3LTRup_R2_B07_04_ABI24.ab1 1 CACTAAATCT TTTATTTTAT CTATGGCTCC GGATCCTTAT ACAGGGCGTA CACTTTCCCT 61 TCTCAATCTC TCATTCCTCC TTCTCTCTCT GCATCTTGGG GCAAAGCAGT AGGTCAGGCA 121 GCATATCACA AAAATTCCAT TTACTGAGAT TAAGGTAATG GCCCAGGATG GGAGCAGGTT 181 ATCAGGAAAA TGCTCTTGCT TGGTTGTATT CCAGTTGAAG GTCTGATTCA CTCTTAAATG 241 TCCATACTTG ATGAGACACA TGAAGCTGTG GTTGGTTGTC ATATTGAAAT CCAGTTTGCT 301 GCTAACAGCA TAGAGCTCAG TTTCAGGATC TTGGGAAACT GTTGTGTTGA TGGCATTTAA 361 TTCTTCTCCA TTTTCCAACC AGGAGAGGTG AGGCTCTGGA AAACCTCCAG AGGTTGAGCA 421 AATTATCCTT CTAATATTAG AAGTTGGAAT TTCAAAGTCA GATATACTAG GTGTAGGGAA 481 GTCAGCTTTG ACTGATAACG TCACTTCAGC CAGGTGTTCC CGCTTGAAAG CGTCTTTTTC 541 ATACTTCAGA ACAACACACT CGTATGTGCC CTCGTCAGAT GGGCGCAGAG CCAGGATCAC 601 AATGGAGAGG TTATTAGTGA TATCAAAGAT GGTCCGGTTC TTGTACTCGG GCCATATATT 661 CATGTCCCCA GACATCATAG TCAGCACCAT TTTCTTCTCC TTTTGCCAGT AGATGCGAGT 721 TTGTGCCAGC TCTTCAACAG AAACATTGTG ACCACAGGAC AGCGTTGCCA CTTCTTTCAC 781 TTCCTTGGTC ACGTGGATAA CACCTGAACA GAAGTGAGAA AGACCAGCCA GCACCAAGAG 841 CTGAAAGAAA TTGAGGTATG GACACTTTGG ATGGTGATGT TCCCCTGCCT CCGTGTGTGG 901 CCCCATGGTG TTCTAGAAGG AATGGTCCAC CCCCCCGGGG TTCGGCAGCC TTTCACGTGG 961 GGCGGCCGTG TATCAGCCTG CGATGCCGGT CTTACTTTGA AGGCCGGTGG GGGGTGGTTC 1021 AAGC //
Primer: M13fwd Sequence file: RDB15968_A8F6c.seq
>02977_D5679_A8F6_1_M13fwd_E01_05_ABI08.ab1 1 GTGGAGGGAT TCTAATACAT CCCAAACTCA AATATATAAA GCATTTGACT TGTTCTATGC 61 CCTAGGGGGC GGGGGGAAGC TAAGCCAGCT TTTTTTAACA TTTAAAATGT TAATTCCATT 121 TTAAATGCAC AGATGTTTTT ATTTCATAAG GGTTTCAATG TGCATGAATG CTGCAATATT 181 CCTGTTACCA AAGCTAGTAT AAATAAAAAT AGATAAACGT GGAAATTACT TAGAGTTTCT 241 GTCATTAACG TTTCCTTCCT CAGTTGACAA CATAAATGCG CTGCTGAGAA GCCAGTTTGC 301 ATCTGTCAGG ATCAATTTCC CATTATGCCA GTCATATTAA TTACTAGTCA ATTAGTTGAT 361 TTTTATTTTT GACATATACA TGTGAAAGAC CCCACCTGTA GGTTTGGCAA GCTAGCTTAA 421 GTAACGCCAT TTTGCAAGGC ATGGAAAAAT ACATAACTGA GAATAGAAAA GTTCAGATCA 481 AGGTCAGGAA CAGATGGAAC AGCTGAATAT GGGCCAAACA GGATATCTGT GGTAAGCAGT 541 TCCTGCCCCG GCTCAGGGCC AAGAACAGAT GGAACAGCTG AATATGGGCC AAACAGGATA 601 TCTGTGGTAA GCAGTTCCTG CCCCGGCTCA GGGCCAAGAA CAGATGGTCC CCAGATGCGG 661 TCCAGCCCTC AGCAGTTTCT AGAGAACCAT CAGATGTTTC CAGGGTGCCC CAAGGACCTG 721 AAATGACCCT GTGCCTTATT TGAACTAACC AATCAGTTCG CTTCTCGCTT CTGTTCGCGC 781 GCTTCTGCTC CCCGAGCTCA ATAAAAGAGC CCACAACCCC TCACTCGGCG CGCCAGTCCT 841 CCGATTGACT GAGTCGCCCG GGTACCCGTG TATCCAATAA ACCCTCTTGC AGTTGCATCC 901 GACTTGTGGG TCTCGCTGTT CCTTGGGAGG GTCTCCTCTG AGTGATTGAC TACCCGTCAG 961 CGGGGGGTCT TTCATTTGGG GGGCTCGTCC GGATCGGGAG ACCCCCTGCC CAGGGACCCA 1021 CCGACCCACC ACCGGGAAGG TAGCTGGGCC AGCACTTTAT CTGTGTCTGT CGATTGTCTA 1081 GTTGTCTATG ACCTGATTTA ATGCCGCTGC CGTTCGGTAC TAGGTTAGGC CTTAACCCTA 1141 TAAGGC //
Primer: pMFG_1 Sequence file: RDB15968_A8F6d.seq
>02977_D5679_A8F6_1_pMFG_1_F01_06_ABI08.ab1 1 AGAGGGGGGA AAAAGAGGGG CTGGTTTAGT AACTCTTGTC ATGTCAGGGT CAGGGAAGTT 61 TACAAGGGGC GGGGGTGCCC CATATAAGAT CTCATATGGC CATATGGGGG CGCCTAGAGA 121 AGGAGTGAGG GCTGGATAAA GGGAGGATCG AGGCGGGGTC GAACGAGGAG GTTCAAGGGG 181 GAGAGACGGG GCGGATGGAG GAAGAGGAGG CGGAGGCTTA GGGTGTACAA AGGGCTTGAC 241 CCAGGGAGGG GGGTCAAAAG CCAAGGCTTC CCAGGTCACG ATGTAGGGGA CCTGGTCTGG 301 GTGTCCATGC GGGCCAGGTG AAAAGACCTT GATCTTAACC TGGGTGATGA GGTCTCGGTT 361 AAAGGTGCCG TCTCGCGGCC ATCCGACGTT AAAGGTTGGC CATTCTGCAG AGCAGAAGGT 421 AACCCAACGT CTCTTCTTGA CATCTACCGA CTGGTTGTGA GCGATCCGCT CGACATCTTT 481 CCAGTGACCT AAGGTCAAAC TTAAGGGAGT GGTAACAGTC TGGCCCGGGC CCATATTTTC 541 AGACAAATAC AGAAACACAG TCAGACAGAG ACAACACAGA ACGATGCTGC AGCAGACAAG 601 ACGCGCGGCG CGGCTTCGGT CCCAAACCGA AAGCAAAAAT TCAGACGGAG GCGGGAACTG 661 TTTTAGGTTC TCGTCTCCTA CCAGAACCAC ATATCCCTCC TCTAAGG //
Primer: Reverse2 Sequence file: RDB15968_A8F6e.seq
>02977_D5679_A8F6_1_Reverse2_E07_13_ABI24.ab1 1 CGTGAAGGCA TTACGATTGG TCGACCAATT CCAAGCTAAG TGAAAGACTC TGTCTCAAAA 61 ACCAACCTGA CACCTGAGCC GGAGCGTTGC CTCTCACTAC CATAAATATA TATACACAAC 121 ACACACACAC ACACACACAC ACACACACAC ACACACACAC ACACAATCAT ACACATAAAC 181 ACAATCACAC ACACACACAT GCACAATCAC ACACACACAC ATCAATCAAT CAATCAATCA 241 ATATACCAAA AATGATAGCT CATACCTGTA ATCTTAGATG TGGGAAGGCT AAAACAGCAG 301 GCTACCCATG ACTTCAAGGT CACCCTGGGT TACAGAGTAG CTAATAGTCT AGCTTGAACT 361 ATAGTGTAGG ATCTTTAAAA AAAAATTAAC CAACCAACCA ACAAACAAAC AAACAAACAA 421 CAACAACAAC AAATGGAAGA CAGAGGACAA AAAAAAATAA AAGAAAAAGT AGAAAGCCAA 481 TGGAGATACT ATCTTAAAAT TAGAAATATT TATGACACAT TCTGAATACT CCATGTTTAT 541 TTCAAGGAAG CCAATGTAAC TTTAAGTACT ATGGCCATTT AATGTAAATA CTTAAGAAAA 601 AAAACCAAAT TAATTTTGAT ACATGCTGCA TGTGAAAGAC CCCCGCTGAC GGGTAGTCAA 661 TCACTCAGAG GAGACCCTCC CAAGGAACAG CGAGACCACA AGTCGGATGC AACTGCAAGA 721 GGGTTTATTG GATACACGGG TACCCGGGCG ACTCAGTCAA TCGGAGGACT GGCGCCCCGA 781 GTGAGGGGTT GTGGGCTCTT TTATTGAGCT CGGGGAGCAG AAGCGCGCGA ACAGAAGCGA 841 GAAGCGAACT GATTGGTTTA GTTCAAATAA GGCACAGGGT CATTTCAGTC CTTGGGGCAC 901 CCTGGAAACA TCTGATGGGT TCTCTAGAAA ACTGCTGAAG GGCTGAACCG CATCTGGGGA 961 CATCTGTTCT TGGCCTGGTC GGGCAGACCT GCTACACAGG AATTCTGTTT GCCCAATATT 1021 CAGCTGTCAT CGTCCTAGCC TGGACGCAGA ACTGGCTACC AGAAA //