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pTcf7mtluc

Reporter plasmid carring TCF-binding consensus sequence

Catalog number RDB02969
Resource name pTcf7mtluc
Clone info. Reporter plasmid carring 7 repeats the TCF-binding mutant sequence upsteam of the IFN-beta basal promoter followed the luciferase geme. p55Bluc was made by inserting the minimam promoter sequences of human IFNbeta gene into the MCS of PGV-B (TOYO B-Net CO., LTD). Expression was confirmed by depositor as follows: reporter assay.
Use as a negative control of pTcf7wtluc (cat.# RDB02968).
Vector backbone p55Bluc (Plasmid)
Size of vector backbone 5,700 bp
Selectable markers Amp^r
Gene/insert name firefly (Photinus pyralis) luciferase cDNA
Depositor Shimotohno, Kunitada |

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Terms and conditions set forth by the DEPOSITOR This material is provided with written consent of Depositor. Acknowledgement to Depositor in publication.
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Remarks ((Additional form)) Approval Form(FormD)
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Distribution information (domestic users) [open/close]


Catalog # Resource name Shipping form Fee (non-profit org.)
RDB02969 pTcf7mtluc DNA solution

Ordering Information [in Japanese] [in English]

References and tips

Electronic file

Electronic file Map RDB02969.gif from Depositor

Featured content

Featured content Promoter Collection (Outline) (English text)
Featured content Promoter Collection (Outline) (Japanese text)
Featured content Control Vectors (English text)
Featured content Wnt signaling pathway (English text)

References

original Ueda, Y., Wnt/beta-catenin signaling suppresses apoptosis in low serum medium and induces morphologic change in rodent fibroblasts. Int. J. Cancer, 99 (5): 681-688 (2002). PMID 12115501.

User reports and related articles (go to bottom)


Sequence information

This clone will be sequenced a portion and digested by restriction enzyme for examination.


References

References

original Ueda, Y., Wnt/beta-catenin signaling suppresses apoptosis in low serum medium and induces morphologic change in rodent fibroblasts. Int. J. Cancer, 99 (5): 681-688 (2002). PMID 12115501.
user report Tanaka, N., APC Mutations as a Potential Biomarker for Sensitivity to Tankyrase Inhibitors in Colorectal Cancer. Mol. Cancer Ther. 16 (4): 752-762 (2017). PMID 28179481 .

2018.12.11

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