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pTcf7mtluc (#RDB02969)

Reporter plasmid carring TCF-binding consensus sequence


Drawn by SnapGene® software
Sequence information
(Assembled from experimentally sequenced data)		 GenBank Flat File Format open
SnapGene file download
Aditional Information
Publication Ueda, Y., Int. J. Cancer, 99 (5): 681-688 (2002). PMID 12115501. [PubMed] [Article] [RRC of NBRP]
Test sheet Data Sheet open 
 
Clone info. Reporter plasmid carring 7 repeats the TCF-binding mutant sequence upsteam of the IFN-beta basal promoter followed the luciferase geme. p55Bluc was made by inserting the minimam promoter sequences of human IFNbeta gene into the MCS of PGV-B (TOYO B-Net CO., LTD). Expression was confirmed by depositor as follows: reporter assay.
Comment Use as a negative control of pTcf7wtluc (RDB02968).
Vector backbone p55Bluc (Plasmid)
Size of vector backbone 5,700 bp
Selectable markers Amp^r
Growth conditions 37 C, LB+Amp
Gene/insert name firefly (Photinus pyralis) luciferase cDNA
Depositor|Developer Shimotohno, Kunitada |

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Ordering forms
Order form [Credit Card Payment] [Bank Transfer Payment] [Example of order form ]
MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ]
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Please visit Information of Request for Distribution.[link] 
Terms and conditions for distribution The research materials will be provided to both non-profit and for-profit organizations. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to DEPOSITOR and a citation of the literature designated by the DEPOSITOR are requested. (Ueda, Y. et al., Int. J. Cancer, 99 (5):681-688, 2002)
提供案内 (日本国内) [open/close]

必要書類
提供依頼書  [依頼書の記入例 ]
提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ]
提供同意書 (MTA, 営利目的用)[Word]
手続きの概要は、「提供申込みについて[link]」をご覧ください。
MTAに書く使用条件 本件研究材料は、非営利機関及び営利機関の区別なく提供する。利用者は、研究成果の公表にあたって寄託者への謝辞の表明を必要とし、寄託者の指定する文献を引用すること(Ueda, Y. et al., Int. J. Cancer, 99 (5):681-688, 2002)。

Catalog # Resource name Shipping form Fee
RDB02969 pTcf7mtluc DNA solution Please contact us.

How to cite this biological resource

Materials & Methods section:

The pTcf7mtluc was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB02969).

Reference section:

Ueda, Y., Hijikata, M., Takagi, S., Takada, R., Takada, S., Chiba, T., Shimotohno, K., Wnt/beta-catenin signaling suppresses apoptosis in low serum medium and induces morphologic change in rodent fibroblasts. Int. J. Cancer, 99 (5): 681-688 (2002). PMID 12115501. [PubMed] [Article] [RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

References

Original, user report and related articles

original Ueda, Y., Wnt/beta-catenin signaling suppresses apoptosis in low serum medium and induces morphologic change in rodent fibroblasts. Int. J. Cancer, 99 (5): 681-688 (2002). PMID 12115501. [PubMed] [Article] [RRC of NBRP]
user_report Tanaka, N., APC Mutations as a Potential Biomarker for Sensitivity to Tankyrase Inhibitors in Colorectal Cancer. Mol. Cancer Ther. 16 (4): 752-762 (2017). PMID 28179481. [PubMed] [Article] [RRC of NBRP]