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Please review the QC test results indicated by check icon below as well as clone information before placing your order.

pTcf7mtluc

Reporter plasmid carring TCF-binding consensus sequence

Catalog number RDB02969
Resource name pTcf7mtluc
Clone info. Reporter plasmid carring 7 repeats the TCF-binding mutant sequence upsteam of the IFN-beta basal promoter followed the luciferase geme. p55Bluc was made by inserting the minimam promoter sequences of human IFNbeta gene into the MCS of PGV-B (TOYO B-Net CO., LTD). Expression was confirmed by depositor as follows: reporter assay.
Comment Use as a negative control of pTcf7wtluc (cat.# RDB02968).
Vector backbone p55Bluc (Plasmid)
Size of vector backbone 5,700 bp
Selectable markers Amp^r
Gene/insert name firefly (Photinus pyralis) luciferase cDNA
Depositor|Developer Shimotohno, Kunitada |
Other clones in our bank
External Database
firefly (Photinus pyralis) luciferase
            Reference sequence
              
             
            Remarks, protocol and/or map (gif) RDB02969.gif

            Distribution information

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            Terms and conditions for distribution This material is provided with written consent of Depositor. Acknowledgement to Depositor in publication.
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            Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word]
            Approval form by depositor [Word]
            Remarks ((Additional form)) Approval Form(FormD)
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            提供同意書 (MTA、非営利機関による非営利学術研究用)[Word]
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            RDB02969 pTcf7mtluc DNA solution

            Please review the QC test results indicated by check icon below as well as clone information before placing your order.

            How to cite this biological resource

            Materials & Methods section:

            The pTcf7mtluc was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB02969).

            Reference section:

            Ueda, Y., Hijikata, M., Takagi, S., Takada, R., Takada, S., Chiba, T., Shimotohno, K., Wnt/beta-catenin signaling suppresses apoptosis in low serum medium and induces morphologic change in rodent fibroblasts. Int. J. Cancer, 99 (5): 681-688 (2002). PMID 12115501.

            Further references such as user reports and related articles (go to bottom)

            References and tips

            Featured content

            Featured content Promoter Collection (Outline) (English text)
            Featured content Promoter Collection (Outline) (Japanese text)
            Featured content Wnt signaling pathway (English text)

            QC test results

            RIKEN BRC has sequenced portions of this material for quality test.
            Please review the QC test results indicated by check icon as well as clone information before placing your order.

            Test sheet RDB17506_A9Ibp1-1.pdf check

            Nucleotide sequence of a portion of this resource (if available).

            Primer: GL2 (Pr0198)
            Region: IFN-b basal pro,Tcf motiff mtx7,SV40p(A),f1 ori
            Sequence file: RDB17506_A9Iba.seq check
            >02969_17506_A9Ib_GL2_C02_08_ABI24.ab1
                1 NNNNNNNNNN NNNAGCTTGN NNNNGNTGCA GTTAGAATGT CCTTTCTCCN TGGGTATGGC
               61 CTATTTATAT GAGATGGTCC TCTCTCTATT CAGCGGATCT CGAGGGCCAA AGGCGGGGCC
              121 AAAGGTAGGG CCAAAGGGCG GGCCAAAGGC GCGGCCAAAG GTAGGGCCAA AGGGCGGGCC
              181 AAAGGACGCG TAAGAGCTCG GTACCTCCCG GGTTATGTTA GCTCAGTTAC AGTACCATAA
              241 GATACATTGA TGAGTTTGGA CAAACCACAA CTAGAATGCA GTGAAAAAAA TGCTTTATTT
              301 GTGAAATTTG TGATGCTATT GCTTTATTTG TAACCATTAT AAGCTGCAAT AAACAAGTTA
              361 ACAACAACAA TTGCATTCAT TTTATGTTTC AGGTTCAGGG GGAGGTGTGG GAGGTTTTTT
              421 AAAGCAAGTA AAACCTCCAC GTACCTTAAT ATTACTTACT TATCATGGTA GCTTGGGCTG
              481 GCGTAATAGC GAAGAGGCCC GCACCGATCG CCCTTCCCAA CAGTTGCGCA GCCTGAATGG
              541 CGAATGGCAA ATTGTAAGCG TTAATATTTT GTTAAAATTC GCGTTAAATT TTTGTTAAAT
              601 CAGCTCATTT TTTAACCAAT AGGCCGAAAT CGGCAAAATC CCTTATAAAT CAAAAGAATA
              661 GACCGAGATA GGGTTGAGTG TTGTTCCAGT TTGGAACAAG AGTCCACTAT TAAAGAACGT
              721 GGACTCCAAC GTCAAAGGGC GAAAAACCGT CTATCAGGGC GATGGCCCAC TACGTGAACC
              781 ATCACCCTAA TCAAGTTTTT TGGGGTCGAG GTGCCGTAAA GCACTAAATC GNAACCCTAA
              841 AGGGANCCCC CGNNTTANAG CTTGACGGGG AAAGCCGGCG AACGTNGCGA GAAANGAAGG
              901 GAAGAAAGCG AAAGGANCGG GCNCTAGGNN GCTGGNAAGN GNANCGGNCA CGCNGCNCGN
            961 ANNCNCNNNN CCCGNCNNNN NNNNNGNNNN NNTNCNNG
            //
            Primer: GV (Pr0748)
            Region: Tcf motiff mtx7,IFN-b basal pro,Luc
            Sequence file: RDB17506_A9Ibb.seq check
            >02969_17506_A9Ib_pGV_Cw_D02_11_ABI24.ab1
                1 NNNNNNNNNN NNNNNCNCTN NNGCGTCCTT TGGCCCGCCC TTTGGCCCTA CCTTTGGCCG
               61 CGCCTTTGGC CCGCCCTTTG GCCCTACCTT TGGCCCCGCC TTTGGCCCTC GAGATCCGCT
              121 GAATAGAGAG AGGACCATCT CATATAAATA GGCCATACCC ATGGAGAAAG GACATTCTAA
              181 CTGCAACCTT TCGCAAGCTT GGCATTCCGG TACTGTTGGT AAAATGGAAG ACGCCAAAAA
              241 CATAAAGAAA GGCCCGGCGC CATTCTATCC TCTAGAGGAT GGAACCGCTG GAGAGCAACT
              301 GCATAAGGCT ATGAAGAGAT ACGCCCTGGT TCCTGGAACA ATTGCTTTTA CAGATGCACA
              361 TATCGAGGTG AACATCACGT ACGCGGAATA CTTCGAAATG TCCGTTCGGT TGGCAGAAGC
              421 TATGAAACGA TATGGGCTGA ATACAAATCA CAGAATCGTC GTATGCAGTG AAAACTCTCT
              481 TCAATTCTTT ATGCCGGTGT TGGGCGCGTT ATTTATCGGA GTTGCAGTTG CGCCCGCGAA
              541 CGACATTTAT AATGAACGTG AATTGCTCAA CAGTATGAAC ATTTCGCAGC CTACCGTAGT
              601 GTTTGTTTCC AAAAAGGGGT TGCAAAAAAT TTTGAACGTG CAAAAAAAAT TACCAATAAT
              661 CCAGAAAATT ATTATCATGG ATTCTAAAAC GGATTACCAG GGATTTCAGT CGATGTACAC
              721 GTTCGTCACA TCTCATCTAC CTCCCGGTTT TAATGAATAC GATTTTGTAC CAGAGTCCTT
              781 TGATCGTGAC AAAACAATTG CACTGATAAT GAATTCCTCT GGATCTACTG NGTTACCNAA
              841 NGGTGTGGCC CTTCCGCATA GAANTGCCTG CGTCAGATTC TCGCANGCCN GANATNCTAT
              901 TTTTGGNAAT CAAATCATTC CNGANACTGC GATTTTNAGT GNNGNNCCNT TCCATCACGG
            961 NTTTGGAATG NTTACTACNC TCNNNNNNTN GANNTGNNGN NNTNC
            //

            Please visit Sequencing and PCR primers for primer information.

            References

            Original, user report and related articles

            original Ueda, Y., Wnt/beta-catenin signaling suppresses apoptosis in low serum medium and induces morphologic change in rodent fibroblasts. Int. J. Cancer, 99 (5): 681-688 (2002). PMID 12115501.
            user_report Tanaka, N., APC Mutations as a Potential Biomarker for Sensitivity to Tankyrase Inhibitors in Colorectal Cancer. Mol. Cancer Ther. 16 (4): 752-762 (2017). PMID 28179481 .

            2023.05.02

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