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pTcf7wtluc

Reporter plasmid carring TCF-binding consensus sequence.

Catalog number RDB02968
Resource name pTcf7wtluc
Clone info. Reporter plasmid carring 7 repeates of the TCF-binding consensus sequence upstream of the IFN-beta basal promoter followed by the luciferase geme. p55Bluc was made by inserting the minimam promoter sequences of human IFNbeta gene into the MCS of PGV-B (TOYO B-Net CO., LTD). Expression was confirmed by depositor as follows: reporter assay.
Use pTcf7mtluc (cat.# RDB02969) as a negative control.
Vector backbone p55Bluc (Plasmid)
Size of vector backbone 5,700 bp
Selectable markers Amp^r
Gene/insert name firefly (Photinus pyralis) luciferase cDNA
Depositor Shimotohno, Kunitada |

Distribution information

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Terms and conditions set forth by the DEPOSITOR This material is provided with written consent of Depositor. Acknowledgement to Depositor in publication.
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Catalog # Resource name Shipping form Fee (non-profit org.)
RDB02968 pTcf7wtluc DNA solution

Ordering Information [in Japanese] [in English]

References and tips

Electronic file

Electronic file Map RDB02968.gif from Depositor

Featured content

Featured content Promoter Collection (Outline) (English text)
Featured content Promoter Collection (Outline) (Japanese text)
Featured content Control Vectors (English text)
Featured content Wnt signaling pathway (English text)

References

original Ueda, Y., Wnt/beta-catenin signaling suppresses apoptosis in low serum medium and induces morphologic change in rodent fibroblasts. Int. J. Cancer, 99 (5): 681-688 (2002). PMID 12115501.

User reports and related articles (go to bottom)

Sequence information

RIKEN BRC has sequenced portions of this material for quality test. Primers and the results are shown below.

Data are summerized on test sheet below.

Test sheet RDB02968_A3B5.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: pGV-CW RDB02968_A3B5a.seq
>2968_2968_A3B5_pGV-CW_A04_01.ab1
    1 ATACCCGGGA AGTACGAGCT CTTACGCGTC CTTTGATCCG CCCTTTGATC CTACCTTTGA
   61 TCGCGCCTTT GATCCGCCCT TTGATCCTAC CTTTGATCGC GCCTTTGATC CTCGAGATCC
  121 GCTGAATAGA GAGAGGACCA TCTCATATAA ATAGGCCATA CCCATGGAGA AAGGACATTC
  181 TAACTGCAAC CTTTCGCAAG CTTGGCATTC CGGTACTGTT GGTAAAATGG AAGACGCCAA
  241 AAACATAAAG AAAGGCCCGG CGCCATTCTA TCCTCTAGAG GATGGAACCG CTGGAGAGCA
  301 ACTGCATAAG GCTATGAAGA GATACGCCCT GGTTCCTGGA ACAATTGCTT TTACAGATGC
  361 ACATATCGAG GTGAACATCA CGTACGCGGA ATACTTCGAA ATGTCCGTTC GGTTGGCAGA
  421 AGCTATGAAA CGATATGGGC TGAATACAAA TCACAGAATC GTCGTATGCA GTGAAAACTC
  481 TCTTCAATTC TTTATGCCGG TGTTGGGCGC GTTATTTATC GGAGTTGCAG TTGCGCCCGC
  541 GAACGACATT TATAATGAAC GTGAATTGCT CAACAGTATG AACATTTCGC AGCCTACCGT
  601 AGTGTTTGTT TCCAAAAAGG GGTTGCAAAA AATTTTGAAC GTGCAAAAAA AATTACCAAT
  661 AATCCAGAAA ATTATTATCA TGGATTCTAA AACGGATTAC CAGGGATTTC AGTCGATGTA
  721 CACGTTCGTC ACATCTCATC TACCTCCCGG TTTTAATGAA TACGATTTTG TACCAGAGTC
  781 CTTTGATCGT GACAAAACAA TTGCACTGAT AATGAATTCC TCTGGATCTA CTGGGTTACC
  841 TAAGGGTGTG GCCCTTCCGC ATAGAACTGC CTGCGTCAGA TTCTCGCATG CCAGAGATCC
  901 TATTTTTGGC AATCAAATCA TTCCGGATAC TGCGATTTTA AGTGTTGTTC CATTCCATCA
  961 CGGGTTTTGG AATGTTTACT ACACTCGGAT ATTTGATATG TGGGATTTCG AGTCGTCTTA
 1021 ATGTATAGAT TTGAAGAAGG AGCTGTTTTT ACGATCCCTT TCAGGATTAC AAAATCAAAG
 1081 TGCGTTTGCT AGTACCAACC CTAATTCCAA TCCTCGCTGA AAGGCACCTC TGATTGGAAC
1141 AAAATACGGG ACTTTATATC CTTA
//
Primer: pGV-CCW RDB02968_A3B5b.seq
>2968_2968_A3B5_pGV-CCW_B04_04.ab1
    1 ACGCGATCAG GAATGCCAAG CTTGCGAAAG GTTGCAGTTA GAATGTCCTT TCTCCATGGG
   61 TATGGCCTAT TTATATGAGA TGGTCCTCTC TCTATTCAGC GGATCTCGAG GATCAAAGGC
  121 GCGATCAAAG GTAGGATCAA AGGGCGGATC AAAGGCGCGA TCAAAGGTAG GATCAAAGGG
  181 CGGATCAAAG GACGCGTAAG AGCTCGGTAC CTCCCGGGTT ATGTTAGCTC AGTTACAGTA
  241 CCATAAGATA CATTGATGAG TTTGGACAAA CCACAACTAG AATGCAGTGA AAAAAATGCT
  301 TTATTTGTGA AATTTGTGAT GCTATTGCTT TATTTGTAAC CATTATAAGC TGCAATAAAC
  361 AAGTTAACAA CAACAATTGC ATTCATTTTA TGTTTCAGGT TCAGGGGGAG GTGTGGGAGG
  421 TTTTTTAAAG CAAGTAAAAC CTCCACGTAC CTTAATATTA CTTACTTATC ATGGTAGCTT
  481 GGGCTGGCGT AATAGCGAAG AGGCCCGCAC CGATCGCCCT TCCCAACAGT TGCGCAGCCT
  541 GAATGGCGAA TGGCAAATTG TAAGCGTTAA TATTTTGTTA AAATTCGCGT TAAATTTTTG
  601 TTAAATCAGC TCATTTTTTA ACCAATAGGC CGAAATCGGC AAAATCCCTT ATAAATCAAA
  661 AGAATAGACC GAGATAGGGT TGAGTGTTGT TCCAGTTTGG AACAAGAGTC CACTATTAAA
  721 GAACGTGGAC TCCAACGTCA AAGGGCGAAA AACCGTCTAT CAGGGCGATG GCCCACTACG
  781 TGAACCATCA CCCTAATCAA GTTTTTTGGG GTCGAGGTGC CGTAAAGCAC TAAATCGGAA
  841 CCCTAAAGGG AGCCCCCGAT TTAGAGCTTG ACGGGGAAAG CCGGCGAACG TGGCGAGAAA
  901 GGAAGGGAAG AAAGCGAAAG GAGCGGGCGC TAGGGCGCTG GCAAGTGTAG CGGTCACGCT
  961 GCGCGTAACC ACCACACCCG CCGCGCTTAA TGCGCCGCTA CAGGGCGCGT CAGGTGGCAC
 1021 TTTTCGGGGA AATGTGCGCG GACCCCTATT GTTTATTTTC TAAATACATT TCAAATATGT
 1081 ATCCGCTCAT GAGACAAATA ACCCTGATAA ATGCTCCATA TATGGAAAGA GAGTATGAGT
1141 ATTCAACATT CCCGGGTGTT CGCCCCCTAA AATTTC
//

Please visit Sequencing and PCR primers for primer information.

References

References

original Ueda, Y., Wnt/beta-catenin signaling suppresses apoptosis in low serum medium and induces morphologic change in rodent fibroblasts. Int. J. Cancer, 99 (5): 681-688 (2002). PMID 12115501.
user report Tanaka, N., APC Mutations as a Potential Biomarker for Sensitivity to Tankyrase Inhibitors in Colorectal Cancer. Mol. Cancer Ther. 16 (4): 752-762 (2017). PMID 28179481 .

2018.12.11

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