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pTcf7wtluc

Reporter plasmid carring TCF-binding consensus sequence.

Catalog number RDB02968
Resource name pTcf7wtluc
Clone info. Reporter plasmid carring 7 repeates of the TCF-binding consensus sequence upstream of the IFN-beta basal promoter followed by the luciferase geme. p55Bluc was made by inserting the minimam promoter sequences of human IFNbeta gene into the MCS of PGV-B (TOYO B-Net CO., LTD). Expression was confirmed by depositor as follows: reporter assay.
Use pTcf7mtluc (cat.# RDB02969) as a negative control.
Vector backbone p55Bluc (Plasmid)
Size of vector backbone 5,700 bp
Selectable markers Amp^r
Gene/insert name firefly (Photinus pyralis) luciferase cDNA
Depositor Shimotohno, Kunitada |

Distribution information

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Terms and conditions set forth by the DEPOSITOR This material is provided with written consent of Depositor. Acknowledgement to Depositor in publication.
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Catalog # Resource name Shipping form Fee (non-profit org.)
RDB02968 pTcf7wtluc DNA solution

Ordering Information [in Japanese] [in English]

References and tips

Electronic file

Electronic file Map RDB02968.gif from Depositor

Original reference

original Ueda, Y., Wnt/beta-catenin signaling suppresses apoptosis in low serum medium and induces morphologic change in rodent fibroblasts. Int. J. Cancer, 99 (5): 681-688 (2002). PMID 12115501.

Further references such as user reports and related articles (go to bottom)

Featured content

Featured content Promoter Collection (Outline) (English text)
Featured content Promoter Collection (Outline) (Japanese text)
Featured content Control Vectors (English text)
Featured content Wnt signaling pathway (English text)

Sequence information

RIKEN BRC has sequenced portions of this material for quality test. Primers and the results are shown below.

Data are summerized on test sheet below.

Test sheet RDB17505_A9Ibp1-1.pdf

Nucleotide sequence of a portion of this resource (if available).

Region: IFN-b basal pro,Tcf motiff WTx7,SV40p(A),f1 ori | Primer: GL2 | Sequence: RDB17505_A9Iba.seq
>02968_17505_A9Ib_GL2_A02_02_ABI24.ab1
    1 NNNNNNNNNN NNNNNNNNTT GCGAAGGNTG CAGTTAGAAT GTCCTTTCTC CNTGGGTATG
   61 GCCTATTTAT ATGAGATGGT CCTCTCTCTA TTCAGCGGAT CTCGAGGATC AAAGGCGCGA
  121 TCAAAGGTAG GATCAAAGGG CGGATCAAAG GCGCGATCAA AGGTAGGATC AAAGGGCGGA
  181 TCAAAGGACG CGTAAGAGCT CGGTACCTCC CGGGTTATGT TAGCTCAGTT ACAGTACCAT
  241 AAGATACATT GATGAGTTTG GACAAACCAC AACTAGAATG CAGTGAAAAA AATGCTTTAT
  301 TTGTGAAATT TGTGATGCTA TTGCTTTATT TGTAACCATT ATAAGCTGCA ATAAACAAGT
  361 TAACAACAAC AATTGCATTC ATTTTATGTT TCAGGTTCAG GGGGAGGTGT GGGAGGTTTT
  421 TTAAAGCAAG TAAAACCTCC ACGTACCTTA ATATTACTTA CTTATCATGG TAGCTTGGGC
  481 TGGCGTAATA GCGAAGAGGC CCGCACCGAT CGCCCTTCCC AACAGTTGCG CAGCCTGAAT
  541 GGCGAATGGC AAATTGTAAG CGTTAATATT TTGTTAAAAT TCGCGTTAAA TTTTTGTTAA
  601 ATCAGCTCAT TTTTTAACCA ATAGGCCGAA ATCGGCAAAA TCCCTTATAA ATCAAAAGAA
  661 TAGACCGAGA TAGGGTTGAG TGTTGTTCCA GTTTGGAACA AGAGTCCACT ATTAAAGAAC
  721 GTGGACTCNA ACGTCAAAGG GCGAAAAACC GTCTATCAGG GCGATGGCCC ACTACGTGAA
  781 CCATCACCCT AATCAAGTTT TTTGGGGTCG AGGTGCCGTA NNGCACTAAA TCGGANCCNN
  841 AAAGGGANCC CCCGNNTTAN AGCTTGNCGG GGAAAGCCGG CGAANGTGGC GANAAAGGAA
  901 NGGAANAAAG CNAAAGGANC GGGCGCNANG NCGCTNNNAA GNGTAGCGGN CACNNNGCGC
  961 NNAACCNCCA CACCNNCCGC GNTNNANGNN NCNNNNCAGG NNNNGNNNNN NGNNNNTTTN
 1021 NNGGNNANNG NGNNNNNNNN CCNNNNTNNN TNNNTTTNNN NANNNNNNNN NANNNNNNNN
 1081 NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN
1141 NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN N
//
Region: Tcf motiff WTx6,IFN-b basal pro,Luc | Primer: GV | Sequence: RDB17505_A9Ibb.seq
>02968_17505_A9Ib_pGV_Cw_B02_05_ABI24.ab1
    1 NNNNNNNNNN NNNNNCNCTN ANGCGTCCTT TGNTCCGCCC TTTGATCCTA CCTTTGATCG
   61 CGCCTTTGAT CCGCCCTTTG ATCCTACCTT TGATCGCGCC TTTGATCCTC GAGATCCGCT
  121 GAATAGAGAG AGGACCATCT CATATAAATA GGCCATACCC ATGGAGAAAG GACATTCTAA
  181 CTGCAACCTT TCGCAAGCTT GGCATTCCGG TACTGTTGGT AAAATGGAAG ACGCCAAAAA
  241 CATAAAGAAA GGCCCGGCGC CATTCTATCC TCTAGAGGAT GGAACCGCTG GAGAGCAACT
  301 GCATAAGGCT ATGAAGAGAT ACGCCCTGGT TCCTGGAACA ATTGCTTTTA CAGATGCACA
  361 TATCGAGGTG AACATCACGT ACGCGGAATA CTTCGAAATG TCCGTTCGGT TGGCAGAAGC
  421 TATGAAACGA TATGGGCTGA ATACAAATCA CAGAATCGTC GTATGCAGTG AAAACTCTCT
  481 TCAATTCTTT ATGCCGGTGT TGGGCGCGTT ATTTATCGGA GTTGCAGTTG CGCCCGCGAA
  541 CGACATTTAT AATGAACGTG AATTGCTCAA CAGTATGAAC ATTTCGCAGC CTACCGTAGT
  601 GTTTGTTTCC AAAAAGGGGT TGCAAAAAAT TTTGAACGTG CAAAAAAAAT TACNAATAAT
  661 CCAGAAAATT ATTATCATGG ATTCTAAAAC GGATTACCAG GGATTTCAGT CGATGTACAC
  721 GTTCGTCACA TCTCATCTAC CTCCCGGTTT TAATGAATAC GATTTTGTAC CAGAGTCCTT
  781 TGATCGTGAC AAAACAATTG CACTGATAAT GAATTCCTCT GGATCTACTG GGTTACNTAA
  841 NGGTGTGGCC NTTCCGCATA GAACTGCCTG CGTCAGANTC TCGCATGCCN GANATCCNAT
  901 TTTTGGNAAT CAAATCNTTN CGGANACTGC GATTTTAAGT GNNGNNCCNT TCCATCNCGG
  961 NTTNNGAATG NTNACNACNC NNNGANANTN GANNTGNNNN NTNNGNNNNN NNNTNNNNGN
 1021 ATANNNTNNN NNNNNNNANN NNNNTTTTNN NNNNNCCNNN NNNNNNNNNN ANNNNNNNNN
 1081 NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN
1141 NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNN
//

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

original Ueda, Y., Wnt/beta-catenin signaling suppresses apoptosis in low serum medium and induces morphologic change in rodent fibroblasts. Int. J. Cancer, 99 (5): 681-688 (2002). PMID 12115501.
user report Tanaka, N., APC Mutations as a Potential Biomarker for Sensitivity to Tankyrase Inhibitors in Colorectal Cancer. Mol. Cancer Ther. 16 (4): 752-762 (2017). PMID 28179481 .

2019.10.11

GNP_filter3_RDBDEP_html_190927.pl