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Please review the QC test results indicated by check icon below as well as clone information before placing your order.

pTcf7wtluc

Reporter plasmid carring TCF-binding consensus sequence.

Catalog number RDB02968
Resource name pTcf7wtluc
Clone info. Reporter plasmid carring 7 repeates of the TCF-binding consensus sequence upstream of the IFN-beta basal promoter followed by the luciferase geme. p55Bluc was made by inserting the minimam promoter sequences of human IFNbeta gene into the MCS of PGV-B (TOYO B-Net CO., LTD). Expression was confirmed by depositor as follows: reporter assay.
Comment Use pTcf7mtluc (cat.# RDB02969) as a negative control.
Vector backbone p55Bluc (Plasmid)
Size of vector backbone 5,700 bp
Selectable markers Amp^r
Gene/insert name firefly (Photinus pyralis) luciferase cDNA
Depositor|Developer Shimotohno, Kunitada |
Other clones in our bank
External Database
firefly (Photinus pyralis) luciferase
            Reference sequence
              
             
            Remarks, protocol and/or map (gif) RDB02968.gif

            Distribution information

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            Terms and conditions for distribution This material is provided with written consent of Depositor. Acknowledgement to Depositor in publication.
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            Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word]
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            Remarks ((Additional form)) Approval Form(FormD)
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            RDB02968 pTcf7wtluc DNA solution

            Please review the QC test results indicated by check icon below as well as clone information before placing your order.

            How to cite this biological resource

            Materials & Methods section:

            The pTcf7wtluc was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB02968).

            Reference section:

            Ueda, Y., Hijikata, M., Takagi, S., Takada, R., Takada, S., Chiba, T., Shimotohno, K., Wnt/beta-catenin signaling suppresses apoptosis in low serum medium and induces morphologic change in rodent fibroblasts. Int. J. Cancer, 99 (5): 681-688 (2002). PMID 12115501.

            Further references such as user reports and related articles (go to bottom)

            References and tips

            Featured content

            Featured content Promoter Collection (Outline) (English text)
            Featured content Promoter Collection (Outline) (Japanese text)
            Featured content Wnt signaling pathway (English text)

            QC test results

            RIKEN BRC has sequenced portions of this material for quality test.
            Please review the QC test results indicated by check icon as well as clone information before placing your order.

            Test sheet RDB17505_A9Ibp1-1.pdf check

            Nucleotide sequence of a portion of this resource (if available).

            Primer: GL2 (Pr0198)
            Region: IFN-b basal pro,Tcf motiff WTx7,SV40p(A),f1 ori
            Sequence file: RDB17505_A9Iba.seq check
            >02968_17505_A9Ib_GL2_A02_02_ABI24.ab1
                1 NNNNNNNNNN NNNNNNNNTT GCGAAGGNTG CAGTTAGAAT GTCCTTTCTC CNTGGGTATG
               61 GCCTATTTAT ATGAGATGGT CCTCTCTCTA TTCAGCGGAT CTCGAGGATC AAAGGCGCGA
              121 TCAAAGGTAG GATCAAAGGG CGGATCAAAG GCGCGATCAA AGGTAGGATC AAAGGGCGGA
              181 TCAAAGGACG CGTAAGAGCT CGGTACCTCC CGGGTTATGT TAGCTCAGTT ACAGTACCAT
              241 AAGATACATT GATGAGTTTG GACAAACCAC AACTAGAATG CAGTGAAAAA AATGCTTTAT
              301 TTGTGAAATT TGTGATGCTA TTGCTTTATT TGTAACCATT ATAAGCTGCA ATAAACAAGT
              361 TAACAACAAC AATTGCATTC ATTTTATGTT TCAGGTTCAG GGGGAGGTGT GGGAGGTTTT
              421 TTAAAGCAAG TAAAACCTCC ACGTACCTTA ATATTACTTA CTTATCATGG TAGCTTGGGC
              481 TGGCGTAATA GCGAAGAGGC CCGCACCGAT CGCCCTTCCC AACAGTTGCG CAGCCTGAAT
              541 GGCGAATGGC AAATTGTAAG CGTTAATATT TTGTTAAAAT TCGCGTTAAA TTTTTGTTAA
              601 ATCAGCTCAT TTTTTAACCA ATAGGCCGAA ATCGGCAAAA TCCCTTATAA ATCAAAAGAA
              661 TAGACCGAGA TAGGGTTGAG TGTTGTTCCA GTTTGGAACA AGAGTCCACT ATTAAAGAAC
              721 GTGGACTCNA ACGTCAAAGG GCGAAAAACC GTCTATCAGG GCGATGGCCC ACTACGTGAA
              781 CCATCACCCT AATCAAGTTT TTTGGGGTCG AGGTGCCGTA NNGCACTAAA TCGGANCCNN
              841 AAAGGGANCC CCCGNNTTAN AGCTTGNCGG GGAAAGCCGG CGAANGTGGC GANAAAGGAA
              901 NGGAANAAAG CNAAAGGANC GGGCGCNANG NCGCTNNNAA GNGTAGCGGN CACNNNGCGC
              961 NNAACCNCCA CACCNNCCGC GNTNNANGNN NCNNNNCAGG NNNNGNNNNN NGNNNNTTTN
             1021 NNGGNNANNG NGNNNNNNNN CCNNNNTNNN TNNNTTTNNN NANNNNNNNN NANNNNNNNN
            1081 NN
            //
            Primer: GV (Pr0748)
            Region: Tcf motiff WTx6,IFN-b basal pro,Luc
            Sequence file: RDB17505_A9Ibb.seq check
            >02968_17505_A9Ib_pGV_Cw_B02_05_ABI24.ab1
                1 NNNNNNNNNN NNNNNCNCTN ANGCGTCCTT TGNTCCGCCC TTTGATCCTA CCTTTGATCG
               61 CGCCTTTGAT CCGCCCTTTG ATCCTACCTT TGATCGCGCC TTTGATCCTC GAGATCCGCT
              121 GAATAGAGAG AGGACCATCT CATATAAATA GGCCATACCC ATGGAGAAAG GACATTCTAA
              181 CTGCAACCTT TCGCAAGCTT GGCATTCCGG TACTGTTGGT AAAATGGAAG ACGCCAAAAA
              241 CATAAAGAAA GGCCCGGCGC CATTCTATCC TCTAGAGGAT GGAACCGCTG GAGAGCAACT
              301 GCATAAGGCT ATGAAGAGAT ACGCCCTGGT TCCTGGAACA ATTGCTTTTA CAGATGCACA
              361 TATCGAGGTG AACATCACGT ACGCGGAATA CTTCGAAATG TCCGTTCGGT TGGCAGAAGC
              421 TATGAAACGA TATGGGCTGA ATACAAATCA CAGAATCGTC GTATGCAGTG AAAACTCTCT
              481 TCAATTCTTT ATGCCGGTGT TGGGCGCGTT ATTTATCGGA GTTGCAGTTG CGCCCGCGAA
              541 CGACATTTAT AATGAACGTG AATTGCTCAA CAGTATGAAC ATTTCGCAGC CTACCGTAGT
              601 GTTTGTTTCC AAAAAGGGGT TGCAAAAAAT TTTGAACGTG CAAAAAAAAT TACNAATAAT
              661 CCAGAAAATT ATTATCATGG ATTCTAAAAC GGATTACCAG GGATTTCAGT CGATGTACAC
              721 GTTCGTCACA TCTCATCTAC CTCCCGGTTT TAATGAATAC GATTTTGTAC CAGAGTCCTT
              781 TGATCGTGAC AAAACAATTG CACTGATAAT GAATTCCTCT GGATCTACTG GGTTACNTAA
              841 NGGTGTGGCC NTTCCGCATA GAACTGCCTG CGTCAGANTC TCGCATGCCN GANATCCNAT
              901 TTTTGGNAAT CAAATCNTTN CGGANACTGC GATTTTAAGT GNNGNNCCNT TCCATCNCGG
              961 NTTNNGAATG NTNACNACNC NNNGANANTN GANNTGNNNN NTNNGNNNNN NNNTNNNNGN
            1021 ATANNNTNNN NNNNNNN
            //

            Please visit Sequencing and PCR primers for primer information.

            References

            Original, user report and related articles

            original Ueda, Y., Wnt/beta-catenin signaling suppresses apoptosis in low serum medium and induces morphologic change in rodent fibroblasts. Int. J. Cancer, 99 (5): 681-688 (2002). PMID 12115501.
            user_report Tanaka, N., APC Mutations as a Potential Biomarker for Sensitivity to Tankyrase Inhibitors in Colorectal Cancer. Mol. Cancer Ther. 16 (4): 752-762 (2017). PMID 28179481 .

            2023.05.02

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