Reporter plasmid carring TCF-binding consensus sequence.
Catalog number | RDB02968 |
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Resource name | pTcf7wtluc |
Clone info. | Reporter plasmid carring 7 repeates of the TCF-binding consensus sequence upstream of the IFN-beta basal promoter followed by the luciferase geme. p55Bluc was made by inserting the minimam promoter sequences of human IFNbeta gene into the MCS of PGV-B (TOYO B-Net CO., LTD). Expression was confirmed by depositor as follows: reporter assay. Use pTcf7mtluc (cat.# RDB02969) as a negative control. |
Vector backbone | p55Bluc (Plasmid) |
Size of vector backbone | 5,700 bp |
Selectable markers | Amp^r |
Gene/insert name | firefly (Photinus pyralis) luciferase cDNA |
Depositor|Developer | Shimotohno, Kunitada | |
Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Terms and conditions set forth by the DEPOSITOR | This material is provided with written consent of Depositor. Acknowledgement to Depositor in publication. |
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Ordering | Please visit Information of Request for Distribution.[link] Order form [Credit Card Payment ![]() ![]() Material Transfer Agreement (MTA) [Word ![]() Approval form by depositor [Word ![]() |
Remarks | ((Additional form)) Approval Form(FormD) Please obtain written approval of the depositor. |
Catalog # | Resource name | Shipping form | Fee (non-profit org.) |
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RDB02968 | pTcf7wtluc | DNA solution |
Electronic file
Electronic file | Map RDB02968.gif
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Original reference
original | Ueda, Y., Wnt/beta-catenin signaling suppresses apoptosis in low serum medium and induces morphologic change in rodent fibroblasts. Int. J. Cancer, 99 (5): 681-688 (2002). PMID 12115501. |
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Further references such as user reports and related articles (go to bottom)
Featured content
Featured content | Promoter Collection (Outline) (English text) |
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Featured content | Promoter Collection (Outline) (Japanese text) |
Featured content | Control Vectors (English text) |
Featured content | Wnt signaling pathway (English text) |
RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by icon as well as clone information before placing your order.
Test sheet | RDB17505_A9Ibp1-1.pdf ![]() |
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Nucleotide sequence of a portion of this resource (if available).
Primer: GL2 Region: IFN-b basal pro,Tcf motiff WTx7,SV40p(A),f1 ori Sequence file: RDB17505_A9Iba.seq ![]() |
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>02968_17505_A9Ib_GL2_A02_02_ABI24.ab1 1 NNNNNNNNNN NNNNNNNNTT GCGAAGGNTG CAGTTAGAAT GTCCTTTCTC CNTGGGTATG 61 GCCTATTTAT ATGAGATGGT CCTCTCTCTA TTCAGCGGAT CTCGAGGATC AAAGGCGCGA 121 TCAAAGGTAG GATCAAAGGG CGGATCAAAG GCGCGATCAA AGGTAGGATC AAAGGGCGGA 181 TCAAAGGACG CGTAAGAGCT CGGTACCTCC CGGGTTATGT TAGCTCAGTT ACAGTACCAT 241 AAGATACATT GATGAGTTTG GACAAACCAC AACTAGAATG CAGTGAAAAA AATGCTTTAT 301 TTGTGAAATT TGTGATGCTA TTGCTTTATT TGTAACCATT ATAAGCTGCA ATAAACAAGT 361 TAACAACAAC AATTGCATTC ATTTTATGTT TCAGGTTCAG GGGGAGGTGT GGGAGGTTTT 421 TTAAAGCAAG TAAAACCTCC ACGTACCTTA ATATTACTTA CTTATCATGG TAGCTTGGGC 481 TGGCGTAATA GCGAAGAGGC CCGCACCGAT CGCCCTTCCC AACAGTTGCG CAGCCTGAAT 541 GGCGAATGGC AAATTGTAAG CGTTAATATT TTGTTAAAAT TCGCGTTAAA TTTTTGTTAA 601 ATCAGCTCAT TTTTTAACCA ATAGGCCGAA ATCGGCAAAA TCCCTTATAA ATCAAAAGAA 661 TAGACCGAGA TAGGGTTGAG TGTTGTTCCA GTTTGGAACA AGAGTCCACT ATTAAAGAAC 721 GTGGACTCNA ACGTCAAAGG GCGAAAAACC GTCTATCAGG GCGATGGCCC ACTACGTGAA 781 CCATCACCCT AATCAAGTTT TTTGGGGTCG AGGTGCCGTA NNGCACTAAA TCGGANCCNN 841 AAAGGGANCC CCCGNNTTAN AGCTTGNCGG GGAAAGCCGG CGAANGTGGC GANAAAGGAA 901 NGGAANAAAG CNAAAGGANC GGGCGCNANG NCGCTNNNAA GNGTAGCGGN CACNNNGCGC 961 NNAACCNCCA CACCNNCCGC GNTNNANGNN NCNNNNCAGG NNNNGNNNNN NGNNNNTTTN 1021 NNGGNNANNG NGNNNNNNNN CCNNNNTNNN TNNNTTTNNN NANNNNNNNN NANNNNNNNN 1081 NN // |
Primer: GV Region: Tcf motiff WTx6,IFN-b basal pro,Luc Sequence file: RDB17505_A9Ibb.seq ![]() |
>02968_17505_A9Ib_pGV_Cw_B02_05_ABI24.ab1 1 NNNNNNNNNN NNNNNCNCTN ANGCGTCCTT TGNTCCGCCC TTTGATCCTA CCTTTGATCG 61 CGCCTTTGAT CCGCCCTTTG ATCCTACCTT TGATCGCGCC TTTGATCCTC GAGATCCGCT 121 GAATAGAGAG AGGACCATCT CATATAAATA GGCCATACCC ATGGAGAAAG GACATTCTAA 181 CTGCAACCTT TCGCAAGCTT GGCATTCCGG TACTGTTGGT AAAATGGAAG ACGCCAAAAA 241 CATAAAGAAA GGCCCGGCGC CATTCTATCC TCTAGAGGAT GGAACCGCTG GAGAGCAACT 301 GCATAAGGCT ATGAAGAGAT ACGCCCTGGT TCCTGGAACA ATTGCTTTTA CAGATGCACA 361 TATCGAGGTG AACATCACGT ACGCGGAATA CTTCGAAATG TCCGTTCGGT TGGCAGAAGC 421 TATGAAACGA TATGGGCTGA ATACAAATCA CAGAATCGTC GTATGCAGTG AAAACTCTCT 481 TCAATTCTTT ATGCCGGTGT TGGGCGCGTT ATTTATCGGA GTTGCAGTTG CGCCCGCGAA 541 CGACATTTAT AATGAACGTG AATTGCTCAA CAGTATGAAC ATTTCGCAGC CTACCGTAGT 601 GTTTGTTTCC AAAAAGGGGT TGCAAAAAAT TTTGAACGTG CAAAAAAAAT TACNAATAAT 661 CCAGAAAATT ATTATCATGG ATTCTAAAAC GGATTACCAG GGATTTCAGT CGATGTACAC 721 GTTCGTCACA TCTCATCTAC CTCCCGGTTT TAATGAATAC GATTTTGTAC CAGAGTCCTT 781 TGATCGTGAC AAAACAATTG CACTGATAAT GAATTCCTCT GGATCTACTG GGTTACNTAA 841 NGGTGTGGCC NTTCCGCATA GAACTGCCTG CGTCAGANTC TCGCATGCCN GANATCCNAT 901 TTTTGGNAAT CAAATCNTTN CGGANACTGC GATTTTAAGT GNNGNNCCNT TCCATCNCGG 961 NTTNNGAATG NTNACNACNC NNNGANANTN GANNTGNNNN NTNNGNNNNN NNNTNNNNGN 1021 ATANNNTNNN NNNNNNN // |
Please visit Sequencing and PCR primers for primer information.
Original, user report and related articles
2020.10.02
GNP_filter3_RDBDEP_html_200316.pl