Resource data sheet
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Please review the QC test results indicated by check icon below as well as clone information before placing your order.

pCMV-3010 (#RDB02966)

The expression plasmid for all hepatitis C viral proteins

Clone info. The expression plasmid for all hepatitis C viral proteins. pKS(+)/CMV was made by inserting the CMV early promoter and SV40 polyA addition signal sequences into the MCS of pBluescriptII KS(+). Expression was confirmed by depositor as follows: Western blotting.
Vector backbone pKS(+)/CMV (Plasmid)
Size of vector backbone 4,200 bp
Selectable markers Amp^r
Gene/insert name hepatitis C virus entire genome cDNA
Reference of insert sequence D90208, D00757, D16435 (DDBJ accession number)
Depositor|Developer Shimotohno, Kunitada |
 
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Distribution information

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MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ]
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Terms and conditions for distribution This material is provided with written consent of Depositor. Acknowledgement to Depositor in publication.
Remarks ((Additional form)) Approval Form(FormD)
Please obtain written approval of the depositor.
提供案内 (日本国内) [open/close]

必要書類
提供依頼書  [依頼書の記入例 ]
提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ]
寄託者による提供承諾書 [Word]
手続きの概要は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。
MTAに書く使用条件 寄託者に許可をとること。研究結果の発表時、謝辞に寄託者名を入れること。
備考 【追加提供依頼書】承諾書(FormD)
寄託者の書面による提供承諾が必要です。

Catalog # Resource name Shipping form Fee (non-profit org.)
RDB02966 pCMV-3010 DNA solution

Please review the QC test results indicated by check icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The pCMV-3010 was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB02966).

Reference section:

Marusawa, H., Hijikata, M., Chiba, T., Shimotohno, K., Hepatitis C virus core protein inhibits Fas- and tumor necrosis factor alpha-mediated apoptosis via NF-kappaB activation. J. Virol., 73, 4713-4720 (1999). PMID 10233931. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB12275_A3Jh.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer:
Sequence file: RDB12275_A3Jha.seq check
>02966_12275_A3Jh_CMV-Foward_A02_02.ab1
    1 CGTACTCGCC CATTGACGCA AATGGGCGGT AGGCGTGTAC GGTGGGAGGT CTATATAAGC
   61 AGAGCTCGTT TAGTGAACCG TCAGATCGCC TGGAGACGCC ATCCACGCTG TTTTGACCTC
  121 CATAGAAGAC ACCGGGACCG ATCCAGCCTC CGCGGCCGGG AACGGTGCAT TGGAACGGAC
  181 CTGCAGCCCA AGCTCGGATC CCCCGGGCTG CAGGAATTCC GTGTTGGGTC GCGAAAGGCC
  241 TTGTGGTACT GCCTGATAGG GTGCTTGCGA GTGCCCCGGG AGGTCTCGTA GACCGTGCAT
  301 CATGAGCACA AATCCTAAAC CTCAAAGAAA AACCAAACGT AACACCAACC GCCGCCCACA
  361 GGACGTTAAG TTCCCGGGCG GTGGTCAGAT CGTTGGTGGA GTTTACCTGT TGCCGCGCAG
  421 GGGCCCCAGG TTGGGTGTGC GCGCGACTAG GAAGACTTCC GAGCGGTCGC AACCTCGTGG
  481 AAGGCGACAA CCTATCCCCA AGGCTCGCCG GCCCGAGGGT AGGACCTGGG CTCAGCCCGG
  541 GTACCCTTGG CCCCTCTATG GCAACGAGGG TATGGGGTGG GCAGGATGGC TCCTGTCACC
  601 CCGTGGCTCT CGGCCTAGTT GGGGCCCCAC AGACCCCCGG CGTAGGTCGC GTAATTTGGG
661 TAAGGTC
//
Primer:
Sequence file: RDB12275_A3Jhb.seq check
>02966_12275_A3Jh_reverse_B02_05.ab1
    1 AGTGCCAATT ACCTCACTAA AGGGAACAAA AGCTGGGTAC CGGGCCCCCC CTCGACGCTC
   61 TCCCTTATGC GACTCCTGCA TTAGGAAGCA GCCCAGTAGT AGGTTGAGGC CGTTGAGCAC
  121 CGCCGCCGCA AGGAATGGTG CATGCAAGGA GATGGCGCCC AACAGTCCCC CGGCCACGGG
  181 GCCTGCCACC ATACCCACGC CGAAACAAGC GCTCATGAGC CCGAAGTGGC GAGCCCGATC
  241 TTCCCCATCG GTGATGTCGG CGATATAGGC GCCAGCAACC GCACCTGTGG CGCCGGTGAT
  301 GCCGGCCACG ATGCGTCCGG CGTAGAGGAT CATAATCAGC CATACCACAT TTGTAGAGGT
  361 TTTACTTGCT TTAAAAAACC TCCCACACCT CCCCCTGAAC CTGAAACATA AAATGAATGC
  421 AATTGTTGTT GTTAACTTGT TTATTGCAGC TTATAATGGT TACAAATAAA GCAATAGCAT
  481 CACAAATTTC ACAAATAAAG CATTTTTTTC ACTGCATTCT AGTTGTGGTT TGTCCAAACT
  541 CATCAATGTA TCTTATCATG TCTGGATCAC GGTATCGATA AGCTTGTCGA CGAATTCCGA
  601 AAAAAAAAAA AGGGAATGGC CTATTGGCCT GGAGTGGTTA GCTCCCCGTT CACCGGTTGG
661 GGAGCAGGTA GA
//

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

original Marusawa, H., Hepatitis C virus core protein inhibits Fas- and tumor necrosis factor alpha-mediated apoptosis via NF-kappaB activation. J. Virol., 73, 4713-4720 (1999). PMID 10233931. [link to RRC of NBRP]