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Please review the QC test results indicated by check icon below as well as clone information before placing your order.

pCMV-3010

The expression plasmid for all hepatitis C viral proteins

Catalog number RDB02966
Resource name pCMV-3010
Clone info. The expression plasmid for all hepatitis C viral proteins. pKS(+)/CMV was made by inserting the CMV early promoter and SV40 polyA addition signal sequences into the MCS of pBluescriptII KS(+). Expression was confirmed by depositor as follows: Western blotting.
Vector backbone pKS(+)/CMV (Plasmid)
Size of vector backbone 4,200 bp
Selectable markers Amp^r
Gene/insert name hepatitis C virus entire genome cDNA
Reference of insert sequence D90208, D00757, D16435 (DDBJ accession number)
Depositor|Developer Shimotohno, Kunitada |
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External Database
hepatitis C virus entire genome
            Reference sequence
              
             
            Remarks, protocol and/or map (gif) RDB02966.gif

            Distribution information

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            Remarks ((Additional form)) Approval Form(FormD)
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            提供条件 寄託者に許可をとること。研究結果の発表時、謝辞に寄託者名を入れること。
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            手続きの概要は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。
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            寄託者の書面による提供承諾が必要です。

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            RDB02966 pCMV-3010 DNA solution

            Please review the QC test results indicated by check icon below as well as clone information before placing your order.

            How to cite this biological resource

            Materials & Methods section:

            The pCMV-3010 was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB02966).

            Reference section:

            Marusawa, H., Hijikata, M., Chiba, T., Shimotohno, K., Hepatitis C virus core protein inhibits Fas- and tumor necrosis factor alpha-mediated apoptosis via NF-kappaB activation. J. Virol., 73, 4713-4720 (1999). PMID 10233931. [link to RRC of NBRP]

            Further references such as user reports and related articles (go to bottom)

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            QC test results

            RIKEN BRC has sequenced portions of this material for quality test.
            Please review the QC test results indicated by check icon as well as clone information before placing your order.

            Test sheet RDB12275_A3Jh.pdf check

            Nucleotide sequence of a portion of this resource (if available).

            Primer:
            Sequence file: RDB12275_A3Jha.seq check
            >02966_12275_A3Jh_CMV-Foward_A02_02.ab1
                1 CGTACTCGCC CATTGACGCA AATGGGCGGT AGGCGTGTAC GGTGGGAGGT CTATATAAGC
               61 AGAGCTCGTT TAGTGAACCG TCAGATCGCC TGGAGACGCC ATCCACGCTG TTTTGACCTC
              121 CATAGAAGAC ACCGGGACCG ATCCAGCCTC CGCGGCCGGG AACGGTGCAT TGGAACGGAC
              181 CTGCAGCCCA AGCTCGGATC CCCCGGGCTG CAGGAATTCC GTGTTGGGTC GCGAAAGGCC
              241 TTGTGGTACT GCCTGATAGG GTGCTTGCGA GTGCCCCGGG AGGTCTCGTA GACCGTGCAT
              301 CATGAGCACA AATCCTAAAC CTCAAAGAAA AACCAAACGT AACACCAACC GCCGCCCACA
              361 GGACGTTAAG TTCCCGGGCG GTGGTCAGAT CGTTGGTGGA GTTTACCTGT TGCCGCGCAG
              421 GGGCCCCAGG TTGGGTGTGC GCGCGACTAG GAAGACTTCC GAGCGGTCGC AACCTCGTGG
              481 AAGGCGACAA CCTATCCCCA AGGCTCGCCG GCCCGAGGGT AGGACCTGGG CTCAGCCCGG
              541 GTACCCTTGG CCCCTCTATG GCAACGAGGG TATGGGGTGG GCAGGATGGC TCCTGTCACC
              601 CCGTGGCTCT CGGCCTAGTT GGGGCCCCAC AGACCCCCGG CGTAGGTCGC GTAATTTGGG
            661 TAAGGTC
            //
            Primer:
            Sequence file: RDB12275_A3Jhb.seq check
            >02966_12275_A3Jh_reverse_B02_05.ab1
                1 AGTGCCAATT ACCTCACTAA AGGGAACAAA AGCTGGGTAC CGGGCCCCCC CTCGACGCTC
               61 TCCCTTATGC GACTCCTGCA TTAGGAAGCA GCCCAGTAGT AGGTTGAGGC CGTTGAGCAC
              121 CGCCGCCGCA AGGAATGGTG CATGCAAGGA GATGGCGCCC AACAGTCCCC CGGCCACGGG
              181 GCCTGCCACC ATACCCACGC CGAAACAAGC GCTCATGAGC CCGAAGTGGC GAGCCCGATC
              241 TTCCCCATCG GTGATGTCGG CGATATAGGC GCCAGCAACC GCACCTGTGG CGCCGGTGAT
              301 GCCGGCCACG ATGCGTCCGG CGTAGAGGAT CATAATCAGC CATACCACAT TTGTAGAGGT
              361 TTTACTTGCT TTAAAAAACC TCCCACACCT CCCCCTGAAC CTGAAACATA AAATGAATGC
              421 AATTGTTGTT GTTAACTTGT TTATTGCAGC TTATAATGGT TACAAATAAA GCAATAGCAT
              481 CACAAATTTC ACAAATAAAG CATTTTTTTC ACTGCATTCT AGTTGTGGTT TGTCCAAACT
              541 CATCAATGTA TCTTATCATG TCTGGATCAC GGTATCGATA AGCTTGTCGA CGAATTCCGA
              601 AAAAAAAAAA AGGGAATGGC CTATTGGCCT GGAGTGGTTA GCTCCCCGTT CACCGGTTGG
            661 GGAGCAGGTA GA
            //

            Please visit Sequencing and PCR primers for primer information.

            References

            Original, user report and related articles

            original Marusawa, H., Hepatitis C virus core protein inhibits Fas- and tumor necrosis factor alpha-mediated apoptosis via NF-kappaB activation. J. Virol., 73, 4713-4720 (1999). PMID 10233931. [link to RRC of NBRP]

            2023.08.18

            GNP_filter3_RDBDEP_html_230702.pl