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Rat alpha1-antitrypsin-AVRR mutant (#RDB02940)

Expression vector of rat alpha1-antitrypsin

Clone info. Rat alpha1-antitrypsin AVRR mutant contains Ala-Val-Arg-Arg in its reactive site. Expression was confirmed by depositor as follows: Immunoprecipitation and Western blotting (HEK293 cells).
Comment Wild type of rat alpha1-antitrypsin is M32247.
Vector backbone pcDNA3 (Plasmid)
Size of vector backbone 5,446 bp
Selectable markers Amp^r (E. coli), Neo (Mammalian cell)
Gene/insert name Rat alpha1-antitrypsin cDNA
Depositor|Developer Matsuda, Yoshiko | Tsuji, Akihiko |
 

Distribution information

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Order form [Credit Card Payment] [Bank Transfer Payment] [Example of order form ]
MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ]
Please visit Information of Request for Distribution.[link] For for-profit-research purpose, please contact us. 
Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature (Tsuji, A. et al., Protein Eng. 15 (2): 123-130 (2002)) are requested.
提供案内 (日本国内) [open/close]

必要書類
提供依頼書  [依頼書の記入例 ]
提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ]
手続きの概要は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。
MTAに書く使用条件 利用者は、研究成果の公表にあたって文献 (Tsuji, A. et al., Protein Eng. 15 (2): 123-130 (2002)) の引用を必要とする。

Catalog # Resource name Availability Shipping form Fee (non-profit org.)
RDB02940 Rat alpha1-antitrypsin-AVRR mutant Under QC test. Please contact us. DNA solution JPY 9,460 (not-for-profit academic purpose)
plus cost of shipping containers, dry ice (if required) and shipping charge

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How to cite this biological resource

Materials & Methods section:

The Rat alpha1-antitrypsin-AVRR mutant was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB02940).

Reference section:

Tsuji, A., Ikoma, T., Hashimoto, E., Matsuda, Y., Development of selectivity of alpha1-antitrypsin variant by mutagenesis in its reactive site loop against proprotein convertase. A crucial role of the P4 arginine in PACE4 inhibition. Protein Eng. 15 (2): 123-130 (2002). PMID 11917148. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

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References

Original, user report and related articles

original Tsuji, A., Development of selectivity of alpha1-antitrypsin variant by mutagenesis in its reactive site loop against proprotein convertase. A crucial role of the P4 arginine in PACE4 inhibition. Protein Eng. 15 (2): 123-130 (2002). PMID 11917148. [link to RRC of NBRP]