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Rat alpha1-antitrypsin-RXXR mutant

Expression vector of rat alpha1-antitrypsin

Catalog number RDB02939
Resource name Rat alpha1-antitrypsin-RXXR mutant
Alternative name Rat alpha1-PDX
Clone info. Rat alpha1-antitrypsin RXXR mutant contains Arg-Ire-Pro-Arg in its reactive site. Expression was confirmed by depositor as follows: immunoprecipitation and Western blotting (HEK293 cells).
Wild type of rat alpha1-antitrypsin is M32247.
Vector backbone pcDNA3 (Plasmid)
Size of vector backbone 5,446 bp
Selectable markers Amp^r (E. coli), Neo (Mammalian cell)
Gene/insert name Rat alpha1-antitrypsin cDNA
Depositor Matsuda, Yoshiko | Tsuji, Akihiko |
 
Other clones in our bank Rat Serpina1 (KEGG orthology K03984) |

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature (Tsuji, A. et al., J. Biochem., 126, 591-603 (1999)) are requested.
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RDB02939 Rat alpha1-antitrypsin-RXXR mutant Under QC test. Please contact us. DNA solution

check Please wait for results of QC test to be uploaded. This clone will be sequenced a portion for examination.
Ordering Information [in Japanese] [in English]

References and tips

Electronic file

Original reference

original Tsuji, A., Development of selectivity of alpha1-antitrypsin variant by mutagenesis in its reactive site loop against proprotein convertase. A crucial role of the P4 arginine in PACE4 inhibition. Protein Eng. 15 (2): 123-130 (2002). PMID 11917148.
original Tsuji, A., Inactivation of proprotein convertase, PACE4, by alpha1-antitrypsin Portland (alpha1-PDX), a blocker of proteolytic activation of bone morphogenetic protein during embryogenesis: evidence that PACE4 is able to form an SDS-stable acyl intermediate with alpha1-PDX. J. Biochem., 126, 591-603 (1999). PMID 10467177.

Further references such as user reports and related articles (go to bottom)

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Sequence information

check Please wait for results of QC test to be uploaded. This clone will be sequenced a portion for examination.


References

Original, user report and related articles

original Tsuji, A., Development of selectivity of alpha1-antitrypsin variant by mutagenesis in its reactive site loop against proprotein convertase. A crucial role of the P4 arginine in PACE4 inhibition. Protein Eng. 15 (2): 123-130 (2002). PMID 11917148.
original Tsuji, A., Inactivation of proprotein convertase, PACE4, by alpha1-antitrypsin Portland (alpha1-PDX), a blocker of proteolytic activation of bone morphogenetic protein during embryogenesis: evidence that PACE4 is able to form an SDS-stable acyl intermediate with alpha1-PDX. J. Biochem., 126, 591-603 (1999). PMID 10467177.

2020.01.07

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