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Nojima cDNA Library Collection No.13.

cDNA library created by Dr. H. Nojima, No.13. Mouse 10T-1/2

Catalog number RDB02903
Resource name Nojima cDNA Library Collection No.13.
Clone info. pAP3neo cDNA Library Mouse 10T-half. Complexity of original stock, 5.3 M. Titer and volume at storage, 340 Mcfu/ml, 3.5 ml (on 2003.05.09). 7% DMSO solution. Host, MC1061A.
Vector backbone pAP3neo (Plasmid)
Size of vector backbone 5,350 bp
Selectable markers Amp^r (E. coli), Neo^r (mammalian cell)
Gene/insert name Mouse anonymous cDNA
Depositor|Developer Nojima, Hiroshi |
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External Database
Mouse anonymous
            Reference sequence
              
             

            Distribution information

            Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
            Terms and conditions for distribution (1) Cite Kobori et al., 1998 with the following statement: The cDNA library was prepared using pAP3neo vector by the Linker-Primer method (Kobori et al., 1998). Kobori M., Ikeda Y., Nara H., Kumegawa M., Nojima H. and Kawashima H.: Large scale isolation of osteoclast-specific genes by an improved method involving the preparation of a subtracted cDNA libary. Genes Cells 3, 459-475, 1998. (2) Acknowledgement to Hiroshi Nojima in publication. (3) No complaint about the library to the Depositor/Developer is acceptable.
            Ordering Please visit Information of Request for Distribution.[link] For for-profit-research purpose, please contact us. 
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            Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word]
            提供案内 (日本国内) [open/close]

            提供条件 (1) 以下の文章と共に以下の文献を引用すること。The cDNA library was prepared using pAP3neo vector by the Linker-Primer method (Kobori et al., 1998). Kobori M., Ikeda Y., Nara H., Kumegawa M., Nojima H. and Kawashima H.: Large scale isolation of osteoclast-specific genes by an improved method involving the preparation of a subtracted cDNA libary. Genes Cells 3, 459-475, 1998.　(2) Hiroshi Nojima に謝辞を表明すること。(3)使用後、本件リソースの内容について寄託者にクレームをつけないこと。
            提供依頼 手続きの詳細は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。
            提供依頼書 [Word]
            提供同意書 (MTA、非営利機関による非営利学術研究用)[Word]
            遺伝子組換え生物の受入れ確認書が必要です。当室にご請求ください。
            備考 【追加提供依頼書】受入れ確認書(書式J)
            組換え体提供にかかる書式が必要です。

            Catalog # Resource name Availability Shipping form Fee (non-profit org.)
            RDB02903 Nojima cDNA Library Collection No.13. Under QC test. Please contact us. Please contact us. Please contact us.

            check Please wait for results of QC test to be uploaded. This clone will be sequenced a portion for examination.

            How to cite this biological resource

            Materials & Methods section:

            The Nojima cDNA Library Collection No.13. was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB02903).

            Reference section:

            Kobori, M., Ikeda, Y., Nara, H., Kato, M., Kumegawa, M., Nojima, H., Kawashima, H., Large scale isolation of osteoclast-specific genes by an improved method involving the preparation of a subtracted cDNA library. Genes to Cells, 3, 459-475 (1998). PMID 9753427.

            Further references such as user reports and related articles (go to bottom)

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            References

            Original, user report and related articles

            original Kobori, M., Large scale isolation of osteoclast-specific genes by an improved method involving the preparation of a subtracted cDNA library. Genes to Cells, 3, 459-475 (1998). PMID 9753427.
            user_report Komiya, M., A genetic screen to discover SUMOylated proteins in living mammalian cells. Sci. Rep. 7 (1): 17443 (2017). PMID 29234079.

            2023.05.12

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