cDNA library created by Dr. H. Nojima, No.12. REF (rat embryonic fibroblast)
Clone info. | pAP3neo cDNA library REF (rat embryonic fibroblast). Complexity of original stock, 62.0 M. Titer and volume at storage, 210 Mcfu/ml, 3.5 ml (on 2003.08.08). 7% DMSO solution. Host, MC1061A. |
---|---|
Vector backbone | pAP3neo (Plasmid) |
Size of vector backbone | 5,350 bp |
Selectable markers | Amp^r (E. coli), Neo^r (mammalian cell) |
Gene/insert name | Rat anonymous cDNA |
Depositor|Developer | Nojima, Hiroshi | |
Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Ordering forms | Order form [Credit Card Payment] [Bank Transfer Payment] [Example of order form ] MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ] Please visit Information of Request for Distribution.[link] For for-profit-research purpose, please contact us. |
---|---|
Terms and conditions for distribution | (1) Cite Kobori et al., 1998 with the following statement: The cDNA library was prepared using pAP3neo vector by the Linker-Primer method (Kobori et al., 1998). Kobori M., Ikeda Y., Nara H., Kumegawa M., Nojima H. and Kawashima H.: Large scale isolation of osteoclast-specific genes by an improved method involving the preparation of a subtracted cDNA libary. Genes Cells 3, 459-475, 1998. (2) Acknowledgement to Hiroshi Nojima in publication. (3) No complaint about the library to the Depositor/Developer is acceptable. |
必要書類 | 提供依頼書 [依頼書の記入例 ] 提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ] 遺伝子組換え生物の受入れ確認書が必要です。当室にご請求ください。 手続きの概要は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。 |
---|---|
MTAに書く使用条件 | (1) 以下の文章と共に以下の文献を引用すること。The cDNA library was prepared using pAP3neo vector by the Linker-Primer method (Kobori et al., 1998). Kobori M., Ikeda Y., Nara H., Kumegawa M., Nojima H. and Kawashima H.: Large scale isolation of osteoclast-specific genes by an improved method involving the preparation of a subtracted cDNA libary. Genes Cells 3, 459-475, 1998. (2) Hiroshi Nojima に謝辞を表明すること。(3)使用後、本件リソースの内容について寄託者にクレームをつけないこと。 |
備考 | 【追加提供依頼書】受入れ確認書(書式J) 組換え体提供にかかる書式が必要です。 |
Catalog # | Resource name | Availability | Shipping form | Fee (non-profit org.) |
---|---|---|---|---|
RDB02902 | Nojima cDNA Library Collection No.12. | Under QC test. Please contact us. | Please contact us. | Please contact us. |
Materials & Methods section:
The Nojima cDNA Library Collection No.12. was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB02902). |
Reference section:
Kobori, M., Ikeda, Y., Nara, H., Kato, M., Kumegawa, M., Nojima, H., Kawashima, H., Large scale isolation of osteoclast-specific genes by an improved method involving the preparation of a subtracted cDNA library. Genes to Cells, 3, 459-475 (1998). PMID 9753427. |
Further references such as user reports and related articles (go to bottom)
Original, user report and related articles
original | Kobori, M., Large scale isolation of osteoclast-specific genes by an improved method involving the preparation of a subtracted cDNA library. Genes to Cells, 3, 459-475 (1998). PMID 9753427. |
---|
Featured content