Resource data sheet
DNA Bank Top
Please review the QC test results indicated by check icon below as well as clone information before placing your order.

Nojima cDNA Library Collection No.07. (#RDB02897)

cDNA library created by Dr. H. Nojima, No.07. Mouse cartilage

Clone info. pAP3neo cDNA library Mouse Cartilage. Complexity of original stock, 2.6 M. Titer and volume at storage, 200 Mcfu/ml, 3.5 ml (on 2003.04.18). 7% DMSO solution. Host, MC1061A.
Vector backbone pAP3neo (Plasmid)
Size of vector backbone 5,350 bp
Selectable markers Amp^r (E. coli), Neo^r (mammalian cell)
Gene/insert name Mouse anonymous cDNA
Depositor|Developer Nojima, Hiroshi |
 

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Ordering forms
Order form [Credit Card Payment] [Bank Transfer Payment] [Example of order form ]
MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ]
Please visit Information of Request for Distribution.[link] For for-profit-research purpose, please contact us. 
Terms and conditions for distribution (1) Cite Kobori et al., 1998 with the following statement: The cDNA library was prepared using pAP3neo vector by the Linker-Primer method (Kobori et al., 1998). Kobori M., Ikeda Y., Nara H., Kumegawa M., Nojima H. and Kawashima H.: Large scale isolation of osteoclast-specific genes by an improved method involving the preparation of a subtracted cDNA libary. Genes Cells 3, 459-475, 1998. (2) Acknowledgement to Hiroshi Nojima in publication. (3) No complaint about the library to the Depositor/Developer is acceptable.
提供案内 (日本国内) [open/close]

必要書類
提供依頼書  [依頼書の記入例 ]
提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ]
遺伝子組換え生物の受入れ確認書が必要です。当室にご請求ください。
手続きの概要は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。
MTAに書く使用条件 (1) 以下の文章と共に以下の文献を引用すること。The cDNA library was prepared using pAP3neo vector by the Linker-Primer method (Kobori et al., 1998). Kobori M., Ikeda Y., Nara H., Kumegawa M., Nojima H. and Kawashima H.: Large scale isolation of osteoclast-specific genes by an improved method involving the preparation of a subtracted cDNA libary. Genes Cells 3, 459-475, 1998.　 (2) Hiroshi Nojima に謝辞を表明すること。(3)使用後、本件リソースの内容について寄託者にクレームをつけないこと。
備考 【追加提供依頼書】受入れ確認書(書式J)
組換え体提供にかかる書式が必要です。

Catalog # Resource name Availability Shipping form Fee (non-profit org.)
RDB02897 Nojima cDNA Library Collection No.07. Under QC test. Please contact us. Please contact us. Please contact us.

check Please wait for results of QC test to be uploaded. This clone will be sequenced a portion for examination.

How to cite this biological resource

Materials & Methods section:

The Nojima cDNA Library Collection No.07. was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB02897).

Reference section:

Kobori, M., Ikeda, Y., Nara, H., Kato, M., Kumegawa, M., Nojima, H., Kawashima, H., Large scale isolation of osteoclast-specific genes by an improved method involving the preparation of a subtracted cDNA library. Genes to Cells, 3, 459-475 (1998). PMID 9753427.

Further references such as user reports and related articles (go to bottom)

QC test results

References

Original, user report and related articles

original Kobori, M., Large scale isolation of osteoclast-specific genes by an improved method involving the preparation of a subtracted cDNA library. Genes to Cells, 3, 459-475 (1998). PMID 9753427.