Shuttle vector to produce rAd expressing human Sp3
Clone info. | Human Sp3 gene is cloned into the SwaI site of pAxCALNLw. (Direction:forward). |
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Comment | Cre-recombinase is required to remove LNL stuffer sequence. Use COS-TPC method for the production of adenovirus. |
Vector backbone | pAxCALNLw (Cosmid, use packaging extracts for transforming E. coli host) |
Size of vector backbone | 45,995 bp |
Selectable markers | Amp^r |
Gene/insert name | Human Sp3 cDNA |
Depositor|Developer | Yokoyama, Kazunari | |
Additional map |
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Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Ordering forms | Order form [Credit Card Payment] [Bank Transfer Payment] [Example of order form ] MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ] Please visit Information of Request for Distribution.[link] For for-profit-research purpose, please contact us. |
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Terms and conditions for distribution | Manuscripts for the publication shall be made through the discussion between Depositor and Recipient Investigator. Additional terms and conditions: Regarding resources containing CAG promoter: In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation for the CAG promoter (Niwa, H., Yamamura, K., Miyazaki, J., Gene 108 : 193-200, 1991) and an acknowledgment to Dr. Jun-ich Miyazaki of the Osaka University are requested. |
Remarks | Remember that you will be working with samples containing infectious virus. |
必要書類 | 提供依頼書 [依頼書の記入例 ] 提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ] 手続きの概要は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。 |
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MTAに書く使用条件 | 使用にあたっては常に密な連絡をとり、発表等に関しても事前に合意を要する。 MTAに書く付加的使用条件: CAGプロモータを含むリソースについて: 利用者は、研究成果の公表にあたってCAGプロモータの文献 (Niwa, H., Yamamura, K., Miyazaki, J., Gene 108 : 193-200, 1991)を引用し、大阪大学 宮崎純一博士への謝辞の表明を必要とする。 |
備考 | このリソースはウイルス粒子産生用のため、取扱いに注意が必要です。 |
Catalog # | Resource name | Availability | Shipping form | Fee (non-profit org.) |
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RDB02706 | pAxCALNLhSp3 (forward) | Under QC test. Please contact us. | DNA solution |
Materials & Methods section:
The pAxCALNLhSp3 (forward) was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB02706). |
Reference section:
Further references such as user reports and related articles (go to bottom)
Please wait for results of QC test to be uploaded. This clone will be sequenced a portion for examination.
Original, user report and related articles
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