Resource data sheet
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pxCAG

Expression vector driven by CAG promoter

Catalog number RDB02546
Resource name pxCAG
Clone info. pAxCAwt (RDB No. 1678) is digested with Sal I and it is self-ligated with T4 DNA ligase. pCAG is expression vector driven by CAG promoter. The cloning site is as same as pAxCAwt (RDB No. 1678).
Vector backbone pxCAG (Plasmid)
Selectable markers Amp^r
Depositor|Developer Yokoyama, Kazunari |
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External Database
            Reference sequence
              
             
            Remarks, protocol and/or map (gif) RDB02546.gif

            Distribution information

            Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
            Terms and conditions for distribution The RECIPIENT agrees to expressly describe the acknowledgement of the RIKEN BRC as the source of the BIOLOGICAL RESOURCE in any publication.
            Additional terms and conditions:
            Regarding resources containing CAG promoter: In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation for the CAG promoter (Niwa, H., Yamamura, K., Miyazaki, J., Gene 108 : 193-200, 1991) and an acknowledgment to Dr. Jun-ich Miyazaki of the Osaka University are requested.
            Ordering Please visit Information of Request for Distribution.[link] For for-profit-research purpose, please contact us. 
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            Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word]
            提供案内 (日本国内) [open/close]

            提供条件 本件リソースを使用して研究成果を発表する場合、理研BRCから提供されたことを明示する。
            付加的提供条件：
            CAGプロモータを含むリソースについて: 利用者は、研究成果の公表にあたってCAGプロモータの文献 (Niwa, H., Yamamura, K., Miyazaki, J., Gene 108 : 193-200, 1991)を引用し、大阪大学 宮崎純一博士への謝辞の表明を必要とする。
            提供依頼 手続きの詳細は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。
            提供依頼書 [Word]
            提供同意書 (MTA、非営利機関による非営利学術研究用)[Word]

            Catalog # Resource name Availability Shipping form Fee (non-profit org.)
            RDB02546 pxCAG Under QC test. Please contact us. DNA solution

            check Please wait for results of QC test to be uploaded. This clone will be sequenced a portion for examination.

            How to cite this biological resource

            Materials & Methods section:

            The pxCAG was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB02546).

            Reference section:

            Further references such as user reports and related articles (go to bottom)

            References and tips

            Featured content

            QC test results

            check Please wait for results of QC test to be uploaded. This clone will be sequenced a portion for examination.

            References

            Original, user report and related articles

            user_report Araki, Y., Efficient recombinant production in mammalian cells using a novel IR/MAR gene amplification method. PLoS One, 7 (7): e41787 (2012). PMID 22844523.

            2023.05.02

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