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Please review the QC test results indicated by check icon below as well as clone information before placing your order.

pCAcc-Luc+ (#RDB02443)

Expressing luciferase under the control of CAG promoter

Clone info. Expression vector of luciferase gene (delived from 7LvWF) under the control of CAG promoter. DNA sequence is available.
Vector backbone pCAcc (Plasmid)
Size of vector backbone 4.7 kb
Selectable markers Amp^r
Gene/insert name firefly (Photinus pyralis) luciferase genomic DNA
Depositor|Developer Hamada, Hirofumi | Nakamura, Takafumi |
 
Sequence RDB02443z.seq
Additional map

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Ordering forms
Order form [Credit Card Payment] [Bank Transfer Payment] [Example of order form ]
MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ]
Please visit Information of Request for Distribution.[link] 
Terms and conditions for distribution The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit institution for a not-for-profit research. RECIPIENT must contact the DEPOSITOR in the case of application for any patents or commercial use based on the results from the use of the BIOLOGICAL RESOURCE. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested.
Additional terms and conditions:
Regarding resources containing CAG promoter: In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation for the CAG promoter (Niwa, H., Yamamura, K., Miyazaki, J., Gene 108 : 193-200, 1991) and an acknowledgment to Dr. Jun-ich Miyazaki of the Osaka University are requested.
提供案内 (日本国内) [open/close]

必要書類
提供依頼書  [依頼書の記入例 ]
提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ]
手続きの概要は、「提供申込みについて[link]」をご覧ください。
MTAに書く使用条件 非営利機関の非営利学術研究へ提供する。利用者が本件リソースを利用した研究成果に基づき特許申請、商業活動等を行う場合は、利用者は事前に寄託者に連絡する。利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する。利用者は、研究成果の公表にあたって謝辞の表明を必要とする。
MTAに書く付加的使用条件：
CAGプロモータを含むリソースについて: 利用者は、研究成果の公表にあたってCAGプロモータの文献 (Niwa, H., Yamamura, K., Miyazaki, J., Gene 108 : 193-200, 1991)を引用し、大阪大学 宮崎純一博士への謝辞の表明を必要とする。

Catalog # Resource name Shipping form Fee (non-profit org.)
RDB02443 pCAcc-Luc+ DNA solution Please contact us.

Please review the QC test results indicated by check icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The pCAcc-Luc+ was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB02443).

Reference section:

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB16055_A8Hup1.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: pCAG-F
Sequence file: RDB16055_A8Hua.seq check
>02443_16055_A8Hu_3_pCA-bgter_F01_16_ABI24.ab1
    1 AACCCCGGGA GTCCCATAAT TTTTGGCAGA GGGAAAAGAT GGCCGCTCTA GAATTACACG
   61 GCGATCTTTC CGCCCTTCTT GGCCTTTATG AGGATCTCTC TGATTTTTCT TGCGTCGAGT
  121 TTTCCGGTAA GACCTTTCGG TACTTCGTCC ACAAACACAA CTCCTCCGCG CAACTTTTTC
  181 GCGGTTGTTA CTTGACTGGC GACGTAATCC ACGATCTCTT TTTCCGTCAT CGTCTTTCCG
  241 TGCTCCAAAA CAACAACGGC GGCGGGAAGT TCACCGGCGT CATCGTCGGG AAGACCTGCG
  301 ACACCTGCGT CGAAGATGTT GGGGTGTTGG AGCAAGATGG ATTCCAATTC AGCGGGAGCC
  361 ACCTGATAGC CTTTGTACTT AATCAGAGAC TTCAGGCGGT CAACGATGAA GAAGTGTTCG
  421 TCTTCGTCCC AGTAAGCTAT GTCTCCAGAA TGTAGCCATC CATCCTTGTC AATCAAGGCG
  481 TTGGTCGCTT CCGGATTGTT TACATAACCG GACATAATCA TAGGACCTCT CACACACAGT
  541 TCGCCTCTTT GATTAACGCC CAGCGTTTTC CCGGTATCCA GATCCACAAC CTTCGCTTCA
  601 AAAAATGGAA CAACTTTACC GACCGCGCCC GGTTTATCAT CCCCCTCGGG TGTAATCAGA
  661 ATAGCTGATG TAGTCTCAGT GAGCCCATAT CCTTGCCTGA TACCTGGCAG ATGGAACCTC
  721 TTGGCAACCG CTTCCCCGAC TTCCTTAGAG AGGGGAGCGC CACCAGAAGC AATTTCGTGT
  781 AAATTAGATA AATCGTATTT GTCAATCAGA GTGCTTTTGG CGAAGAAGGA GAATAGGGTT
  841 GGCACCAGCA GCGCACTTTG AATCTTGTAA TCCTGAAGGC TCCTCAGAAA CAGCTCTTCT
  901 TCAAATCTAT ACATTAAGAC GACTCGAATC CACATATCAA ATATCCGAGT GTAGTAAACA
  961 TTCCAAAAAC CGTGATGGAA ATGGAAACAA CACTTTAAAA ATCGCAGTAT CCCGGGAAAT
 1021 GATTTTGATT GCCAAAAATT AGGATCCTCC TGGCCATGCG AAGATCTCAA CGGCAAGGCC
1081 AGGTTCTTTG AGGCAGAGAG TC
//
Primer: pCA-bgter
Sequence file: RDB16055_A8Hub.seq check
>02443_16055_A8Hu_3_pCAG-F_D01_10_ABI24.ab1
    1 GCGGGGGGGG CAGGGCTTCC TTCGGGGGGG GAGGGGGCAG GGCGGGGGTT CGGCTTCTGG
   61 CGTGTGACCG GCGGCTCTAG AGCCTCTGCT AACCATGTTC ATGCCTTCTT CTTTTTCCTA
  121 CAGCTCCTGG GCAACGTGCT GGTTATTGTG CTGTCTCATC ATTTTGGCAA AGAATTCCTG
  181 CAGCCCGGGG GATCTGCGAT CTAAGTAAGC TTGGCATTCC GGTACTGTTG GTAAAGCCAC
  241 CATGGAAGAC GCCAAAAACA TAAAGAAAGG CCCGGCGCCA TTCTATCCGC TGGAAGATGG
  301 AACCGCTGGA GAGCAACTGC ATAAGGCTAT GAAGAGATAC GCCCTGGTTC CTGGAACAAT
  361 TGCTTTTACA GATGCACATA TCGAGGTGGA CATCACTTAC GCTGAGTACT TCGAAATGTC
  421 CGTTCGGTTG GCAGAAGCTA TGAAACGATA TGGGCTGAAT ACAAATCACA GAATCGTCGT
  481 ATGCAGTGAA AACTCTCTTC AATTCTTTAT GCCGGTGTTG GGCGCGTTAT TTATCGGAGT
  541 TGCAGTTGCG CCCGCGAACG ACATTTATAA TGAACGTGAA TTGCTCAACA GTATGGGCAT
  601 TTCGCAGCCT ACCGTGGTGT TCGTTTCCAA AAAGGGGTTG CAAAAAATTT TGAACGTGCA
  661 AAAAAAGCTC CCAAATCATC CAAAAAATTA TTATCATGGA TTCTAAAAAC GGATTACCAG
  721 GGATTTCAGT CGATGTACAC GTTCGTCACA TCTCATCTAC CTCCCGGTTT TAATGAAATA
  781 CGATTTTGTG CCAAGAGTCC TTCGATAGGG ACAAGACAAT TGGCACTGAT CAATGAACTC
  841 CTCTGGATCT ACTGGGTCTG GCCTAAAAGG TGTCGCTCTG CCTCATAGAA ACTGCCCTGC
  901 GTGAGATTTC TCGCATGCCC AGAGATCCCT ATTTTTTGGC AATCCAAAAT CATTCCGGAT
961 ACCTGGCGAA TTTTTAAAGT GGTTGG
//

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

user_report Naka, M., Intracellular Delivery of Plasmid DNA Using Amphipathic Helical Cell-Penetrating Peptides Containing Dipropylglycine. Chem. Pharm. Bull. (Tokyo) 72 (5): 512-517 (2024). PMID 38811213. [link to RRC of NBRP]
user_report Yokoo, H., Development of delivery carriers for plasmid DNA by conjugation of a helical template to oligoarginine. Bioorg. Med. Chem. 72: 116997 (2022). PMID 36088811. [link to RRC of NBRP]
user_report Chen, Q., Polyplex micelle installing intracellular self-processing functionalities without free catiomers for safe and efficient systemic gene therapy through tumor vasculature targeting. Biomaterials 113: 253-265 (2017). PMID 27835820. [link to RRC of NBRP]
user_report Kato, T., Plasmid DNA delivery by arginine-rich cell-penetrating peptides containing unnatural amino acids. Bioorg. Med. Chem. pii: S0968-0896 (16) 30275-9 (2016). PMID 27132868. [link to RRC of NBRP]
user_report Oba, M., Plasmid DNA delivery using fluorescein-labeled arginine-rich peptides. Bioorg. Med. Chem. 23 (15): 4911-4918 (2015). PMID 26048025. [link to RRC of NBRP]
user_report Ge, Z, Targeted gene delivery by polyplex micelles with crowded PEG palisade and cRGD moiety for systemic treatment of pancreatic tumors. Biomaterials, 35 (10): 3416-3426 (2014). PMID 24439417. [link to RRC of NBRP]
user_report Dirisala, A., Optimized rod length of polyplex micelles for maximizing transfection efficiency and their performance in systemic gene therapy against stroma-rich pancreatic tumors. Biomaterials, 35 (20): 5359-5368 (2014). PMID 24720877. [link to RRC of NBRP]
user_report Chen Q., Homo-catiomer integration into PEGylated polyplex micelle from block-catiomer for systemic anti-angiogenic gene therapy for fibrotic pancreatic tumors. Biomaterials, 33 (18): 4722-4730 (2012). PMID 22444644. [link to RRC of NBRP]
user_report Kataoka, K., Charge-conversion ternary polyplex with endosome disruption moiety: a technique for efficient and safe gene delivery. Angew. Chem. Int. Ed. Engl. 47 (28): 5163-5166 (2008). PMID 18528828. [link to RRC of NBRP]
user_report Mickler, F.M., Effect of integrin targeting and PEG shielding on polyplex micelle internalization studied by live-cell imaging. J. Control Release, 156 (3): 364-373 (2011). PMID 21843561. [link to RRC of NBRP]
user_report Vachutinsky Y., Antiangiogenic gene therapy of experimental pancreatic tumor by sFlt-1 plasmid DNA carried by RGD-modified crosslinked polyplex micelles. J. Control. Release (2010). PMID 20138936. [link to RRC of NBRP]
user_report Oba M, Antiangiogenic gene therapy of solid tumor by systemic injection of polyplex micelles loading plasmid DNA encoding soluble flt-1. Mol. Pharm. 7 (2), 501-509 (2010). PMID 20178335. [link to RRC of NBRP]
user_report Oba M., Polyplex micelles prepared from omega-cholesteryl PEG-polycation block copolymers for systemic gene delivery. Biomaterials, 32 (2): 652-663 (2010) PMID 20932567. [link to RRC of NBRP]
user_report Oba M., Polyplex micelles with cyclic RGD peptide ligands and disulfide cross-links directing to the enhanced transfection via controlled intracellular trafficking. Mol. Pharm., 5, 1080-1092 (2008). PMID 19434856. [link to RRC of NBRP]
user_report Oba M., Cyclic RGD peptide-conjugated polyplex micelles as a targetable gene delivery system directed to cells possessing alphavbeta3 and alphavbeta5 integrins. Bioconjug. Chem., 18, 1415-1423 (2007). PMID 17595054. [link to RRC of NBRP]
user_report Nishiyama N., Light-induced gene transfer from packaged DNA enveloped in a dendrimeric photosensitizer. Nat. Mater., 4, 934-941 (2005). PMID 16299510. [link to RRC of NBRP]