Resource data sheet
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pCAcc-Luc+

Expressing luciferase under the control of CAG promoter

Catalog number RDB02443
Resource name pCAcc-Luc+
Clone info. Expression vector of luciferase gene (delived from 7LvWF) under the control of CAG promoter. DNA sequence is available.
Vector backbone pCAcc (Plasmid)
Size of vector backbone 4.7 kb
Selectable markers Amp^r
Gene/insert name firefly (Photinus pyralis) luciferase genomic DNA
Depositor Hamada, Hirofumi | Nakamura, Takafumi |

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Terms and conditions set forth by the DEPOSITOR The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit institution for a not-for-profit research. RECIPIENT must contact the DEPOSITOR in the case of application for any patents or commercial use based on the results from the use of the BIOLOGICAL RESOURCE. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested.
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Catalog # Resource name Shipping form Fee (non-profit org.)
RDB02443 pCAcc-Luc+ DNA solution Please contact us.

Ordering Information [in Japanese] [in English]

References and tips

Electronic file

Electronic file Sequence RDB02443z.seq from Depositor
Electronic file Map RDB02443.gif from Depositor

Featured content

Featured content Control Vectors (English text)

References

User reports and related articles (go to bottom)

Sequence information

RIKEN BRC has sequenced portions of this material for quality test. Primers and the results are shown below.

Data are summerized on test sheet below.

Test sheet RDB16055_A8Hup1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: pCAG-F RDB16055_A8Hua.seq
>02443_16055_A8Hu_3_pCA-bgter_F01_16_ABI24.ab1
    1 AACCCCGGGA GTCCCATAAT TTTTGGCAGA GGGAAAAGAT GGCCGCTCTA GAATTACACG
   61 GCGATCTTTC CGCCCTTCTT GGCCTTTATG AGGATCTCTC TGATTTTTCT TGCGTCGAGT
  121 TTTCCGGTAA GACCTTTCGG TACTTCGTCC ACAAACACAA CTCCTCCGCG CAACTTTTTC
  181 GCGGTTGTTA CTTGACTGGC GACGTAATCC ACGATCTCTT TTTCCGTCAT CGTCTTTCCG
  241 TGCTCCAAAA CAACAACGGC GGCGGGAAGT TCACCGGCGT CATCGTCGGG AAGACCTGCG
  301 ACACCTGCGT CGAAGATGTT GGGGTGTTGG AGCAAGATGG ATTCCAATTC AGCGGGAGCC
  361 ACCTGATAGC CTTTGTACTT AATCAGAGAC TTCAGGCGGT CAACGATGAA GAAGTGTTCG
  421 TCTTCGTCCC AGTAAGCTAT GTCTCCAGAA TGTAGCCATC CATCCTTGTC AATCAAGGCG
  481 TTGGTCGCTT CCGGATTGTT TACATAACCG GACATAATCA TAGGACCTCT CACACACAGT
  541 TCGCCTCTTT GATTAACGCC CAGCGTTTTC CCGGTATCCA GATCCACAAC CTTCGCTTCA
  601 AAAAATGGAA CAACTTTACC GACCGCGCCC GGTTTATCAT CCCCCTCGGG TGTAATCAGA
  661 ATAGCTGATG TAGTCTCAGT GAGCCCATAT CCTTGCCTGA TACCTGGCAG ATGGAACCTC
  721 TTGGCAACCG CTTCCCCGAC TTCCTTAGAG AGGGGAGCGC CACCAGAAGC AATTTCGTGT
  781 AAATTAGATA AATCGTATTT GTCAATCAGA GTGCTTTTGG CGAAGAAGGA GAATAGGGTT
  841 GGCACCAGCA GCGCACTTTG AATCTTGTAA TCCTGAAGGC TCCTCAGAAA CAGCTCTTCT
  901 TCAAATCTAT ACATTAAGAC GACTCGAATC CACATATCAA ATATCCGAGT GTAGTAAACA
  961 TTCCAAAAAC CGTGATGGAA ATGGAAACAA CACTTTAAAA ATCGCAGTAT CCCGGGAAAT
 1021 GATTTTGATT GCCAAAAATT AGGATCCTCC TGGCCATGCG AAGATCTCAA CGGCAAGGCC
1081 AGGTTCTTTG AGGCAGAGAG TC
//
Primer: pCA-bgter RDB16055_A8Hub.seq
>02443_16055_A8Hu_3_pCAG-F_D01_10_ABI24.ab1
    1 GCGGGGGGGG CAGGGCTTCC TTCGGGGGGG GAGGGGGCAG GGCGGGGGTT CGGCTTCTGG
   61 CGTGTGACCG GCGGCTCTAG AGCCTCTGCT AACCATGTTC ATGCCTTCTT CTTTTTCCTA
  121 CAGCTCCTGG GCAACGTGCT GGTTATTGTG CTGTCTCATC ATTTTGGCAA AGAATTCCTG
  181 CAGCCCGGGG GATCTGCGAT CTAAGTAAGC TTGGCATTCC GGTACTGTTG GTAAAGCCAC
  241 CATGGAAGAC GCCAAAAACA TAAAGAAAGG CCCGGCGCCA TTCTATCCGC TGGAAGATGG
  301 AACCGCTGGA GAGCAACTGC ATAAGGCTAT GAAGAGATAC GCCCTGGTTC CTGGAACAAT
  361 TGCTTTTACA GATGCACATA TCGAGGTGGA CATCACTTAC GCTGAGTACT TCGAAATGTC
  421 CGTTCGGTTG GCAGAAGCTA TGAAACGATA TGGGCTGAAT ACAAATCACA GAATCGTCGT
  481 ATGCAGTGAA AACTCTCTTC AATTCTTTAT GCCGGTGTTG GGCGCGTTAT TTATCGGAGT
  541 TGCAGTTGCG CCCGCGAACG ACATTTATAA TGAACGTGAA TTGCTCAACA GTATGGGCAT
  601 TTCGCAGCCT ACCGTGGTGT TCGTTTCCAA AAAGGGGTTG CAAAAAATTT TGAACGTGCA
  661 AAAAAAGCTC CCAAATCATC CAAAAAATTA TTATCATGGA TTCTAAAAAC GGATTACCAG
  721 GGATTTCAGT CGATGTACAC GTTCGTCACA TCTCATCTAC CTCCCGGTTT TAATGAAATA
  781 CGATTTTGTG CCAAGAGTCC TTCGATAGGG ACAAGACAAT TGGCACTGAT CAATGAACTC
  841 CTCTGGATCT ACTGGGTCTG GCCTAAAAGG TGTCGCTCTG CCTCATAGAA ACTGCCCTGC
  901 GTGAGATTTC TCGCATGCCC AGAGATCCCT ATTTTTTGGC AATCCAAAAT CATTCCGGAT
961 ACCTGGCGAA TTTTTAAAGT GGTTGG
//

Please visit Sequencing and PCR primers for primer information.

References

References

user report Chen, Q., Polyplex micelle installing intracellular self-processing functionalities without free catiomers for safe and efficient systemic gene therapy through tumor vasculature targeting. Biomaterials 113: 253-265 (2017). PMID 27835820.
user report Kato, T., Plasmid DNA delivery by arginine-rich cell-penetrating peptides containing unnatural amino acids. Bioorg. Med. Chem. pii: S0968-0896 (16) 30275-9 (2016). PMID 27132868.
user report Oba, M., Plasmid DNA delivery using fluorescein-labeled arginine-rich peptides. Bioorg. Med. Chem. 23 (15): 4911-4918 (2015). PMID 26048025.
user report Ge, Z, Targeted gene delivery by polyplex micelles with crowded PEG palisade and cRGD moiety for systemic treatment of pancreatic tumors. Biomaterials, 35 (10): 3416-3426 (2014). PMID 24439417.
user report Dirisala, A., Optimized rod length of polyplex micelles for maximizing transfection efficiency and their performance in systemic gene therapy against stroma-rich pancreatic tumors. Biomaterials, 35 (20): 5359-5368 (2014). PMID 24720877.
user report Chen Q., Homo-catiomer integration into PEGylated polyplex micelle from block-catiomer for systemic anti-angiogenic gene therapy for fibrotic pancreatic tumors. Biomaterials, 33 (18): 4722-4730 (2012). PMID 22444644.
user report Kataoka, K., Charge-conversion ternary polyplex with endosome disruption moiety: a technique for efficient and safe gene delivery. Angew. Chem. Int. Ed. Engl. 47 (28): 5163-5166 (2008). PMID 18528828.
user report Mickler, F.M., Effect of integrin targeting and PEG shielding on polyplex micelle internalization studied by live-cell imaging. J. Control Release, 156 (3): 364-373 (2011). PMID 21843561.
user report Vachutinsky Y., Antiangiogenic gene therapy of experimental pancreatic tumor by sFlt-1 plasmid DNA carried by RGD-modified crosslinked polyplex micelles. J. Control. Release (2010). PMID 20138936.
user report Oba M, Antiangiogenic gene therapy of solid tumor by systemic injection of polyplex micelles loading plasmid DNA encoding soluble flt-1. Mol. Pharm. 7 (2), 501-509 (2010). PMID 20178335.
user report Oba M., Polyplex micelles prepared from omega-cholesteryl PEG-polycation block copolymers for systemic gene delivery. Biomaterials, 32 (2): 652-663 (2010) PMID 20932567.
user report Oba M., Polyplex micelles with cyclic RGD peptide ligands and disulfide cross-links directing to the enhanced transfection via controlled intracellular trafficking. Mol. Pharm., 5, 1080-1092 (2008). PMID 19434856.
user report Oba M., Cyclic RGD peptide-conjugated polyplex micelles as a targetable gene delivery system directed to cells possessing alphavbeta3 and alphavbeta5 integrins. Bioconjug. Chem., 18, 1415-1423 (2007). PMID 17595054.
user report Nishiyama N., Light-induced gene transfer from packaged DNA enveloped in a dendrimeric photosensitizer. Nat. Mater., 4, 934-941 (2005). PMID 16299510.

2018.12.11

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