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Please review the QC test results indicated by check icon below as well as clone information before placing your order.

pCAGhp53R273H (Forward)

Mammalian vector expressing mutant of p53 gene (R273H)

Catalog number RDB02433
Resource name pCAGhp53R273H (Forward)
Clone info. Mammalian vector expressing mutant of p53 gene
Vector backbone pCAG (Plasmid)
Selectable markers Amp^r
Gene/insert name Human tumor protein p53 cDNA
Depositor|Developer Yokoyama, Kazunari |
Other clones in our bank
External Database
Human tumor protein p53
          Reference sequence
            
           
          Remarks, protocol and/or map (gif) RDB02433.gif

          Distribution information

          Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
          Terms and conditions for distribution Manuscripts for the publication shall be made through the discussion between Depositor and Recipient Investigator.
          Additional terms and conditions:
          Regarding resources containing CAG promoter: In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation for the CAG promoter (Niwa, H., Yamamura, K., Miyazaki, J., Gene 108 : 193-200, 1991) and an acknowledgment to Dr. Jun-ich Miyazaki of the Osaka University are requested.
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          Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word]
          提供案内 (日本国内) [open/close]

          提供条件 使用にあたっては常に密な連絡をとり、発表等に関しても事前に合意を要する。
          付加的提供条件：
          CAGプロモータを含むリソースについて: 利用者は、研究成果の公表にあたってCAGプロモータの文献 (Niwa, H., Yamamura, K., Miyazaki, J., Gene 108 : 193-200, 1991)を引用し、大阪大学 宮崎純一博士への謝辞の表明を必要とする。
          提供依頼 手続きの詳細は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。
          提供依頼書 [Word]
          提供同意書 (MTA、非営利機関による非営利学術研究用)[Word]

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          RDB02433 pCAGhp53R273H (Forward) DNA solution

          Please review the QC test results indicated by check icon below as well as clone information before placing your order.

          How to cite this biological resource

          Materials & Methods section:

          The pCAGhp53R273H (Forward) was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB02433).

          Reference section:

          Further references such as user reports and related articles (go to bottom)

          References and tips

          Featured content

          QC test results

          RIKEN BRC has sequenced portions of this material for quality test.
          Please review the QC test results indicated by check icon as well as clone information before placing your order.

          Test sheet RDB19202_B1G7p1-1.pdf check

          Nucleotide sequence of a portion of this resource (if available).

          Primer: pCAG-F (Pr0328)
          Region: insert 5'
          Sequence file: RDB19202_B1G7a.seq check
          >02433_19202_B1G7_2_pCAG-F_C01_03_ABI08.ab1
              1 NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN GCAGGGCGGG GTTCGGCTTC TGGCGTGTGA
             61 CCGGCGGCTC TANAGCCTCT GCTAACCATG TTCATGCCTT CTTCTTTTTC CTACAGCTCC
            121 TGGGCAACGT GCTGGTTATT GTGCTGTCTC ATCATTTTGG CAAAGAATTG ATTTATCGAT
            181 TTAATTCTAG AGCCACCGTC CAGGGAGCAG GTAGCTGCTG GGCTCCGGGG ACACTTTGCG
            241 TTCGGGCTGG GAGCGTGCTT TCCACGACGG TGACACGCTT CCCTGGATTG GCAGCCAGAC
            301 TGCCTTCCGG GTCACTGCCA TGGAGGAGCC GCAGTCAGAT CCTAGCGTCG AGCCCCCTCT
            361 GAGTCAGGAA ACATTTTCAG ACCTATGGAA ACTACTTCCT GAAAACAACG TTCTGTCCCC
            421 CTTGCCNNNN NNNGCAATGG ATGATTTGAT GCTGTCCCCG GACGATATTG ANNNNNGGTT
            481 CACTGAAGAC CCANGTNNNN ANGAAGCTCC CANANNNNCA GAGGCTGCTC CCCCCGTGGC
            541 CCCTGCACCA NCNNCTCCTA CACCGGCGGC CCCTGCACCA GCCCCCNNCN TGGNCCCNNN
          601 NNNNCTTCNG TCCCTTNNNN NNAAAANCTA NCNNNNNNNN NN
          //
          Primer: pCAGGS_R1 (Pr0614)
          Region: insert 3'
          Sequence file: RDB19202_B1G7b.seq check
          >02433_19202_B1G7_2_pCAGGS_R1_D01_04_ABI08.ab1
              1 NNNNNNNNNN NNNNNNNCNA TTGNNNTGAA AANAAATTTC CTTTATTANN NNNAAGTCAG
             61 ATGCTCAAGG GGCTTCATGA TGTCCCCATA ATTTTTGGCA GAGGGAAAAA GATCTCAGTG
            121 GTATTTGTGA GCCAGGGCAT TGGCCACACC AGCCACCACC TTCTGATAGG CAGCCTGCAC
            181 CTGAGGAGTG AATTGATTAT CGATTCTAGA CTAGTTTAAT TAATTTAATT CCGGGACAAA
            241 GCAAATGGAA GTCCTGGGTG CTTCTGACGC ACACCTATTG CAAGCAAGGG TTCAAAGACC
            301 CAAAACCCAA AATGGCAGGG GAGGGAGAGA TGGGGGTGGG AGGCTGTCAG TGGGGAACAA
            361 GAAGTGGAGA ATGTCANNCN GAGTCANGCC CTTCTGTCNT GAACATGAGT TTTTTATGGC
          421 GGGAGGTAGA NNGNNCCTTT TTGNANTNNN NNNNNNN
          //
          Primer: TP53-M_F (Pr1029)
          Region: insert mid(273aa,H)
          Sequence file: RDB19202_B1G7c.seq check
          >02433_19202_B1G7_2_TP53-M_F_B01_04_ABI24.ab1
              1 NNNNNNNNNN NNNNNNCNNC NNNCNNCTAC NACTACNTGT GTAACAGTTC CTGCATGGGC
             61 GGCATGAACC GGAGGCCCAT CCTCACCATC ATCACACTGG AAGACTCCAG TGGTAATCTA
            121 CTGGGACGGA ACAGCTTTGA GGTGCACGTT TGTGCCTGTC CTGGGAGAGA CCGGCGCACA
            181 GAGGAAGAGA ATCTCCGCAA GAAAGGGGAG CCTCACCACG AGCTGCCCCC AGGGAGCACT
            241 AAGCGAGCAC TGCCCAACAA CACCAGCTCC TCTCCCCAGC CAAAGAAGAA ACCACTGGAT
            301 GGAGAATATT TCACCCTTCA GATCCGTGGG CGTGAGCGCT TCGAGATGTT CCGAGAGCTG
            361 AATGAGGCCT TGGAACTCAA GGATGCCCAG GCTGGGAAGG AGCCAGGGGG GAGCAGGGCT
            421 CACTCCAGCC ACCTGAAGTC CAAAAAGGGT CAGTCTACCT CCCGCCATAA AAAACTCATG
            481 TTCAAGACAG AAGGGCCTGA CTCAGACTGA CATTCTCCAC TTCTTGTTCC CCACTGACAG
            541 CCTCCCACCC CCATCTCTCC CTCCCCTGCC ATTTTGGGTT TTGGGTCTTT GAACCCTTGC
            601 TTGCAATAGG TGTGCGTCAG AAGCACCCAG GACTTCCATT TGCTTTGTCC CGGAATTAAA
            661 TTAATTAAAC TAGTCNAGAA NCNANNNTCN NNTCNCTCCT CNNNGCNNNN TNNNNATCNN
          721 NNNNNNGNNN CNNGNGNNNN NNNNNN
          //

          Please visit Sequencing and PCR primers for primer information.

          References

          Original, user report and related articles

          2023.05.01

          GNP_filter3_RDBDEP_html_230501.pl