Resource data sheet
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pAxCAhIFN-beta (forward)

Shuttle vector to produce rAd expressing human IFN-beta

Catalog number RDB02285
Resource name pAxCAhIFN-beta (forward)
Alternative name 015C3
Clone info. Human IFN-beta gene was cloned in pAxCAwt (RDB No. 1678). Direction : forward.
Produce adenovirus by COS-TPC method.
Vector backbone pAxCAwt (Cosmid, use packaging extracts for transformation)
Size of vector backbone 44,820 bp
Selectable markers Amp^r
Gene/insert name Human IFN-beta cDNA
Reference of nucleotide sequence M28622 (DDBJ accession number)
Depositor DNA Bank, |
 
Other clones in our bank human IFNB1 (NCBI Gene 3456) |

Distribution information

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Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature(s) designated by the DEPOSITOR is requested. The RECIPIENT agrees to use the BIOLOGICAL RESOURCE only for not-for-profit research in the not-for-profit organization.
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RDB02285 pAxCAhIFN-beta (forward) DNA solution

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References and tips

Electronic file

Electronic file Map RDB02285.gif from Depositor

Original reference

Further references such as user reports and related articles (go to bottom)

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Sequence information

RIKEN BRC has sequenced portions of this material for quality test. Primers and the results are shown below.

Data are summerized on test sheet below.

Test sheet RDB14299_A6Htp1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: pAxCA_F1 | Sequence: RDB14299_A6Hta.seq
>02285_14299_A6Ht_pAxCA_F1_F01_16_ABI24.ab1
    1 TAAACGATCT TCTACATGTT CATGCCTTCT TCTTTTTCCT ACAGCTCCTG GGCAACGTGC
   61 TGGTTATTGT GCTGTCTCAT CATTTTGGCA AAGAATTGAT TTATCGATTT GATCCGATTG
  121 TCAACATGAC CAACAAGTGT CTCCTCCAAA TTGCTCTCCT GTTGTGCTTC TCCACTACAG
  181 CTCTTTCCAT GAGCTACAAC TTGCTTGGAT TCCTACAAAG AAGCAGCAAT TTTCAGTGTC
  241 AGAAGCTCCT GTGGCAATTG AATGGGAGGC TTGAATATTG CCTCAAGGAC AGGATGAACT
  301 TTGACATCCC TGAGGAGATT AAGCAGCTGC AGCAGTTCCA GAAGGAGGAC GCCGCATTGA
  361 CCATCTATGA GATGCTCCAG AACATCTTTG CTATTTTCAG ACAAGATTCA TCTAGCACTG
  421 GCTGGAATGA GACTATTGTT GAGAACCTCC TGGCTAATGT CTATCATCAG ATAAACCATC
  481 TGAAGACAGT CCTGGAAGAA AAACTGGAGA AAGAAGATTT CACCAGGGGA AAACTCATGA
  541 GCAGTCTGCA CCTGAAAAGA TATTATGGGA GGATTCTGCA TTACCTGAAG GCCAAGGAGT
  601 ACAGTCACTG TGCCTGGACC ATAGTCAGAG TGGAAATCCT AAGGAACTTT TACTTCATTA
  661 ACAGACTTAC AGGTTACCTC CGAAACTGAA GATCTCCTAG CCTGAATCCA TATGACTAGT
  721 AGATCCTCTA GAAATTAATT AAACTAGTCT AGAATCGATA ATCAATTCAC TCCTCAGGTG
  781 CAGGCTGCCT ATCAGAAGGT GGTGGCTGGT GTGGCCAATG CCCTGGCTCA AAAATACCAC
  841 TGAGATCTTT TTTCCCTCTG CCAAAAATTA TGGGGAACAT CATGAAGCCC CTTGAGCATC
  901 TGACTTTCTG CTAATAAAAG AAAATTTATT ATTCATTGCT ATGGCAATAG TGGTGGTTGG
  961 GAATTTTTTG GTGGTCCTCC TCACCTCGGG AAAGGACAAT ATGGGAAGGG GCCAAATCAA
 1021 TTTAAAACAT CCAGAAATGG AGTATTTGGG TTTAGAGTTG GGCCAACAAT ATGCCCCAAT
 1081 ATGCTGGGCG CGCCAAGGAA CAGGGGTGTC TATATAAAGA GAAGGTCATT CGAGTATATA
 1141 TGGAAACAGC CCCCTTGCTT GTCCCATCCT TACTCCATAG AAAAGAGGCC CTTGGCACAC
1201 TGTGGAGAGT ATCCA
//
Primer: Amp_R | Sequence: RDB14299_A6Htb.seq
>02285_14299_A6Ht_Amp_R_G01_19_ABI24.ab1
    1 CTCGATTCAT ATTATTGAGC ATTTATCAGG GTTATTGTCT CATGAGCGGA TACATATTTG
   61 AATGTATTTA GAAAAATAAA CAAATAGGGG TTCCGCGCAC ATTTCCCCGA AAAGTGCCAC
  121 CTGACGTCTA AGAAACCATT ATTATCATGA CATTAACCTA TAAAAATAGG CGTATCACGA
  181 GGCCCTTTCG TCTTCAAGAA TTCCGGATCC GGGCCAATAT GATAATGAGG GGGTGGAGTT
  241 TGTGACGTGG CGCGGGGCGT GGGAACGGGG CGGGTGACGT AGTAGTGTGG CGGAAGTGTG
  301 ATGTTGCAAG TGTGGCGGAA CACATGTAAG CGACGGATGT GGCAAAAGTG ACGTTTTTGG
  361 TGTGCGCCGG TGTACACAGG AAGTGACAAT TTTCGCGCGG TTTTAGGCGG ATGTTGTAGT
  421 AAATTTGGGC GTAACCGAGT AAGATTTGGC CATTTTCGCG GGAAAACTGA ATAAGAGGAA
  481 GTGAAATCTG AATAATTTTG TGTTACTCAT AGCGCGTAAT ATTTGTCTAG GGCCGCGGGG
  541 ACTTTGACCG TTTACGTGGA GACTCGCCCA GGTGTTTTTC TCAGGTGTTT TCCGCGTTCC
  601 GGGTCAAAGT TGGCGTTTTA TTATTATAGT CAGCATCGTT TAAACAGCTT GGGCCCTCGA
  661 GGGGTCGAGG GATCTCCATA AGAGAAGAGG GACAGCTATG ACTGGGAGTA GTCAGGAGAG
  721 GAGGAAAAAT CTGGCTAGTA AAACATGTAA GGAAAATTTT AGGGATGTTA AAGAAAAAAA
  781 TAACACAAAA CAAAATATAA AAAAAATCTA ACCTCAAGTC AAGGCTTTTC CTATGGAATA
  841 GGAATGGACA GCAGGGGGCT GTTTCATATA CTGATGACCT CTTTAATAGC CAACCTTTGT
  901 TCATGGCAGC CAGCATATGG GCAATATGTT TGCCCAAACT CTAAACAAAT ACTCATCTGA
  961 TGTTTAATGA ATTTGGCCCT CCAATATGTG TCTCGAGTGA AGACACAAAA TCACAACTAT
 1021 TGTCATGAAG ATAAATCTTA TAGCTGAGTC GAGTCTACGG CCTCTGATGT GCCTAATTGC
1081 AAGGAAGATC GAGTC
//

Please visit Sequencing and PCR primers for primer information.


References

Original, user report and related articles

reference Higashi, Y., Structure and expression of a cloned cDNA for mouse interferon-beta. J. Biol. Chem., 258, 9522-9529 (1983). PMID 6688252.

2019.09.18

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