RDB02285 - pAxCAhIFN-beta (forward)

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icon below as well as clone information before placing your order.

pAxCAhIFN-beta (forward) (#RDB02285)

Shuttle vector to produce rAd expressing human IFN-beta

Alternative name	015C3
Clone info.	Human IFN-beta gene was cloned in pAxCAwt (RDB No. 1678). Direction : forward.
Comment	Produce adenovirus by COS-TPC method.
Vector backbone	pAxCAwt (Cosmid, use packaging extracts for transforming E. coli host)
Size of vector backbone	44,820 bp
Selectable markers	Amp^r
Gene/insert name	Human IFN-beta cDNA
Depositor\|Developer	DNA Bank, \|

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Order form [Credit Card Payment] [Bank Transfer Payment] [Example of order form ]
MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ]
Please visit Information of Request for Distribution.[link]

Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature(s) designated by the DEPOSITOR is requested. The RECIPIENT agrees to use the BIOLOGICAL RESOURCE only for not-for-profit research in the not-for-profit organization.
Additional terms and conditions:
Regarding resources containing CAG promoter: In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation for the CAG promoter (Niwa, H., Yamamura, K., Miyazaki, J., Gene 108 : 193-200, 1991) and an acknowledgment to Dr. Jun-ich Miyazaki of the Osaka University are requested.

Remarks Remember that you will be working with samples containing infectious virus.

提供案内 (日本国内) [open/close]

Ordering forms	Order form [Credit Card Payment] [Bank Transfer Payment] [Example of order form ] MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ] Please visit Information of Request for Distribution.[link]
Terms and conditions for distribution	In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature(s) designated by the DEPOSITOR is requested. The RECIPIENT agrees to use the BIOLOGICAL RESOURCE only for not-for-profit research in the not-for-profit organization. Additional terms and conditions: Regarding resources containing CAG promoter: In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation for the CAG promoter (Niwa, H., Yamamura, K., Miyazaki, J., Gene 108 : 193-200, 1991) and an acknowledgment to Dr. Jun-ich Miyazaki of the Osaka University are requested.
Remarks	Remember that you will be working with samples containing infectious virus.

必要書類	提供依頼書 [依頼書の記入例 ] 提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ] 手続きの概要は、「提供申込みについて[link]」をご覧ください。
MTAに書く使用条件	利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する。本件リソースは非営利機関にのみ提供し、非営利研究にのみ利用することができる。 MTAに書く付加的使用条件： CAGプロモータを含むリソースについて: 利用者は、研究成果の公表にあたってCAGプロモータの文献 (Niwa, H., Yamamura, K., Miyazaki, J., Gene 108 : 193-200, 1991)を引用し、大阪大学宮崎純一博士への謝辞の表明を必要とする。
備考	このリソースはウイルス粒子産生用のため、取扱いに注意が必要です。

Catalog #	Resource name	Shipping form	Fee (non-profit org.)
RDB02285	pAxCAhIFN-beta (forward)	DNA solution	JPY 9,460 (not-for-profit academic purpose) plus cost of shipping containers, dry ice (if required) and shipping charge

Please review the QC test results indicated by check

icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The pAxCAhIFN-beta (forward) was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB02285).

Reference section:

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet	RDB14299_A6Htp1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: pAxCA_F1 Sequence file: RDB14299_A6Hta.seq
>02285_14299_A6Ht_pAxCA_F1_F01_16_ABI24.ab1 1 TAAACGATCT TCTACATGTT CATGCCTTCT TCTTTTTCCT ACAGCTCCTG GGCAACGTGC 61 TGGTTATTGT GCTGTCTCAT CATTTTGGCA AAGAATTGAT TTATCGATTT GATCCGATTG 121 TCAACATGAC CAACAAGTGT CTCCTCCAAA TTGCTCTCCT GTTGTGCTTC TCCACTACAG 181 CTCTTTCCAT GAGCTACAAC TTGCTTGGAT TCCTACAAAG AAGCAGCAAT TTTCAGTGTC 241 AGAAGCTCCT GTGGCAATTG AATGGGAGGC TTGAATATTG CCTCAAGGAC AGGATGAACT 301 TTGACATCCC TGAGGAGATT AAGCAGCTGC AGCAGTTCCA GAAGGAGGAC GCCGCATTGA 361 CCATCTATGA GATGCTCCAG AACATCTTTG CTATTTTCAG ACAAGATTCA TCTAGCACTG 421 GCTGGAATGA GACTATTGTT GAGAACCTCC TGGCTAATGT CTATCATCAG ATAAACCATC 481 TGAAGACAGT CCTGGAAGAA AAACTGGAGA AAGAAGATTT CACCAGGGGA AAACTCATGA 541 GCAGTCTGCA CCTGAAAAGA TATTATGGGA GGATTCTGCA TTACCTGAAG GCCAAGGAGT 601 ACAGTCACTG TGCCTGGACC ATAGTCAGAG TGGAAATCCT AAGGAACTTT TACTTCATTA 661 ACAGACTTAC AGGTTACCTC CGAAACTGAA GATCTCCTAG CCTGAATCCA TATGACTAGT 721 AGATCCTCTA GAAATTAATT AAACTAGTCT AGAATCGATA ATCAATTCAC TCCTCAGGTG 781 CAGGCTGCCT ATCAGAAGGT GGTGGCTGGT GTGGCCAATG CCCTGGCTCA AAAATACCAC 841 TGAGATCTTT TTTCCCTCTG CCAAAAATTA TGGGGAACAT CATGAAGCCC CTTGAGCATC 901 TGACTTTCTG CTAATAAAAG AAAATTTATT ATTCATTGCT ATGGCAATAG TGGTGGTTGG 961 GAATTTTTTG GTGGTCCTCC TCACCTCGGG AAAGGACAAT ATGGGAAGGG GCCAAATCAA 1021 TTTAAAACAT CCAGAAATGG AGTATTTGGG TTTAGAGTTG GGCCAACAAT ATGCCCCAAT 1081 ATGCTGGGCG CGCCAAGGAA CAGGGGTGTC TATATAAAGA GAAGGTCATT CGAGTATATA 1141 TGGAAACAGC CCCCTTGCTT GTCCCATCCT TACTCCATAG AAAAGAGGCC CTTGGCACAC 1201 TGTGGAGAGT ATCCA //
Primer: Amp_R Sequence file: RDB14299_A6Htb.seq
>02285_14299_A6Ht_Amp_R_G01_19_ABI24.ab1 1 CTCGATTCAT ATTATTGAGC ATTTATCAGG GTTATTGTCT CATGAGCGGA TACATATTTG 61 AATGTATTTA GAAAAATAAA CAAATAGGGG TTCCGCGCAC ATTTCCCCGA AAAGTGCCAC 121 CTGACGTCTA AGAAACCATT ATTATCATGA CATTAACCTA TAAAAATAGG CGTATCACGA 181 GGCCCTTTCG TCTTCAAGAA TTCCGGATCC GGGCCAATAT GATAATGAGG GGGTGGAGTT 241 TGTGACGTGG CGCGGGGCGT GGGAACGGGG CGGGTGACGT AGTAGTGTGG CGGAAGTGTG 301 ATGTTGCAAG TGTGGCGGAA CACATGTAAG CGACGGATGT GGCAAAAGTG ACGTTTTTGG 361 TGTGCGCCGG TGTACACAGG AAGTGACAAT TTTCGCGCGG TTTTAGGCGG ATGTTGTAGT 421 AAATTTGGGC GTAACCGAGT AAGATTTGGC CATTTTCGCG GGAAAACTGA ATAAGAGGAA 481 GTGAAATCTG AATAATTTTG TGTTACTCAT AGCGCGTAAT ATTTGTCTAG GGCCGCGGGG 541 ACTTTGACCG TTTACGTGGA GACTCGCCCA GGTGTTTTTC TCAGGTGTTT TCCGCGTTCC 601 GGGTCAAAGT TGGCGTTTTA TTATTATAGT CAGCATCGTT TAAACAGCTT GGGCCCTCGA 661 GGGGTCGAGG GATCTCCATA AGAGAAGAGG GACAGCTATG ACTGGGAGTA GTCAGGAGAG 721 GAGGAAAAAT CTGGCTAGTA AAACATGTAA GGAAAATTTT AGGGATGTTA AAGAAAAAAA 781 TAACACAAAA CAAAATATAA AAAAAATCTA ACCTCAAGTC AAGGCTTTTC CTATGGAATA 841 GGAATGGACA GCAGGGGGCT GTTTCATATA CTGATGACCT CTTTAATAGC CAACCTTTGT 901 TCATGGCAGC CAGCATATGG GCAATATGTT TGCCCAAACT CTAAACAAAT ACTCATCTGA 961 TGTTTAATGA ATTTGGCCCT CCAATATGTG TCTCGAGTGA AGACACAAAA TCACAACTAT 1021 TGTCATGAAG ATAAATCTTA TAGCTGAGTC GAGTCTACGG CCTCTGATGT GCCTAATTGC 1081 AAGGAAGATC GAGTC //