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Please review the QC test results indicated by check icon below as well as clone information before placing your order.

mST6GalNACI (#RDB02092)

Plasmid clone of mouse sialyltransferase cDNA

Alternative name ST60-I; GalNAC alpha 2,6-sialyltransferase
Clone info. Plasmid clone of mouse sialyltransferase cDNA
Vector backbone pBluescript II SK(+) (Plasmid)
Size of vector backbone 3.0 kb
Selectable markers Amp^r
Gene/insert name Mouse ST6 (alpha-N-acetyl-neuraminyl-2,3-beta-galactosyl-1,3)-N-acetylgalactosaminide alpha-2,6-sialyltransferase 1 cDNA
Reference of insert sequence Y11274 (DDBJ accession number)
Depositor|Developer Tsuji, Shuichi |
 
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Distribution information

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Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested (Ikehara, Y. et al., Glycobiology, 9, 1213-1224 (1999)).
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提供依頼書  [依頼書の記入例 ]
提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ]
手続きの概要は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。
MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Ikehara, Y. et al., Glycobiology, 9, 1213-1224 (1999))。

Catalog # Resource name Shipping form Fee (non-profit org.)
RDB02092 mST6GalNACI DNA solution

Please review the QC test results indicated by check icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The mST6GalNACI was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB02092).

Reference section:

Ikehara, Y., Kojima, N., Kurosawa, N., Kudo, T., Kono, M., Nishihara, S., Issiki, S., Morozumi, K., Itzkowitz, S., Tsuda, T., Nishimura, S.I., Tsuji, S., Narimatsu, H., Cloning and expression of a human gene encoding an N-acetylgalactosamine-alpha2,6-sialyltransferase (ST6GalNAc I): a candidate for synthesis of cancer-associated sialyl-Tn antigens. Glycobiology, 9, 1213-1224 (1999). PMID 10536037. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB02092_A9I6p1-1.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: reverse-2 (Pr0100)
Region: insert 5'
Sequence file: RDB02092_A9I6a.seq check
>02092_02092_A9I6_3_reverse-2_F01_16_ABI24.ab1
    1 NNNNNNNNNN NNNNNNGNAA CAAAANCTGG AGCTCCACCG CGGTGGCGGC CGCTCTAGAA
   61 CTAGTGGATC CCCCGGGCTG CAGGAATTCG CGGCCGCTTT TTTTTTCACC TGTGCTTTCA
  121 TGAAAGCTAA GAGTCACGCT AAAGGGCAGG GCTCCCTCGC TCTCACCTGA AGGAGCAGGC
  181 GATCTGACTG CTGCTTTAAG AAGCTTGCTC TCATCATACA TGCTGTTAGG GACCAGCCAT
  241 CCACCATGAC GAGATATTGC AGAGGACTTT CCCAGAGGCA AGCTTTCCTG CTTCTGACTG
  301 TGTTGGCATT GCTCTTTATT CTGCTCTTCG TTGTTAAGGA TCCAAGGGCA AAAGATTCCA
  361 GGTGCCAGTT CATATGGAAG AATGACGCAA GTGCCCAGGA GAATCAACAA AAGGCTGAGC
  421 CCCAAGTACC CATCATGACA CTGTCACCCA GAGTTCACAA CAAGGAGACA ACCTCTGTCA
  481 GTTCAAAGGA CCTGAAGAAA CAGGAGAGAG AGGCAGTCCA AGGAGAGCAA GCTGAGGGGA
  541 AGGAGAAGAG GAAGTTAGAG ACCATAAGGC CAGCACCAGA GAATCCTCAG AGCAAGGCAG
  601 AGCCTGCTGC AAAGACGCCT GTGTCAGAAC ACCTGGACAA ACTACCCAGA ACTCCAGGAG
  661 CACTGTCAAC AAGGAAGACA CCAATGGCCA CAGGAGCTGT CCCAGCTAAG AAGAAAGTGN
  721 TCCAGGCTAC CAAATCCCCT GCCTCTTCCC CACACCCCAC CACACGAANA ANGNAAAGGC
  781 TGAANGCCTC TGANTTCAAG TCTGANCCTC GGNGGGATTT TGAGGAGGAA TATAGCTNNN
  841 ATATGANCAG CCNGCAGANG ANNTGCTCTG CTNCTGNNAA GATCAANGNC TNCNANNCAC
901 CANGGNTACN GANTANCTTT CTGNCNNNNA TCNNTCNNNN NCNGGNNNNN NNNNN
//
Primer: pUC-M13_-100 (Pr0310)
Region: insert 3'
Sequence file: RDB02092_A9I6b.seq check
>02092_02092_A9I6_3_pUC-M13_-100_C03_03_ABI08.ab1
    1 NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNGTTTNN NNNNNNNNNG NNGTTGTAAA
   61 ACGACGGCCA GTGNGCGCGC GTAATACGAC TCACTATAGG GCGAATTGGG TACCGGGCCC
  121 CCCCTCGAGA TCTGGAATTC GAGCTCGGTA CCCGTTGAAA GGTGTGGAGC AGGTCTGATT
  181 CCAGAGACAC AGGTAGGAGC ATTTGAGGAG CCCTTGCATT CTGTGTTGTA AGTGGTCGAG
  241 GGGAACACCC TGATCTGGGC CCTGGTCAGT TCTTTGCTTT GTCACTTTGT GGACGCTGGT
  301 AGAGCCAGAT GATGCCTTCA TCATGCAACC GCTTCCACAC CATTCTCTCT AACCTGAAGT
  361 CATGGTTGAT ATAAAAGATG AGCCGTTTCC ATGATGTATC ATAGTAGTGG TCAGAGAAGC
  421 GCTGGTGGCC CTCGGTGATG AAGCCATAGG CGCTGACCTT GTCACAGAGA TGAAGGGCTG
  481 TGAGCAGCAG GAGGGCACCA GTGGTGGGGC GGTATATTCT CCAATGGGCA GTGTCCAGGG
  541 TCTTTGACCT CAGAAACCTG TTCTTCATGT AACGGAGAAA GTCTGGGTGC AGCAGCAAGT
  601 ATCGATCCAA GTCCAAGGCA TTCCGGAAGG CTTCCTGAGG CCTATGCCTG AACCAGGAAA
661 GGTGGGTTTT TGCCAAGGTC TGATTCNA
//

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

original Ikehara, Y., Cloning and expression of a human gene encoding an N-acetylgalactosamine-alpha2,6-sialyltransferase (ST6GalNAc I): a candidate for synthesis of cancer-associated sialyl-Tn antigens. Glycobiology, 9, 1213-1224 (1999). PMID 10536037. [link to RRC of NBRP]