Resource data sheet
DNA Bank Top
Please review the QC test results indicated by check icon below as well as clone information before placing your order.

pT7/NS-CATc (#RDB02055)

Influenza virus chimeric NS-CATc RNA

Alternative name pT7/NS-CATm (old name)
Clone info. Influenza virus NS-CATc chimeric RNA synthesizing plasmid by in vitro transcription
Vector backbone pUC19 (Plasmid)
Size of vector backbone 2.7 kb
Selectable markers Amp^r
Gene/insert name Influenza virus NS-CATc cDNA
Depositor|Developer Nakada, Susumu |
 
Additional map

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Ordering forms
Order form [Credit Card Payment] [Bank Transfer Payment] [Example of order form ]
MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ]
Please visit Information of Request for Distribution.[link] For for-profit-research purpose, please contact us. 
Terms and conditions for distribution Acknowledgement to Depositor and Developer in publication.
提供案内 (日本国内) [open/close]

必要書類
提供依頼書  [依頼書の記入例 ]
提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ]
手続きの概要は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。
MTAに書く使用条件 研究結果の発表時、謝辞に寄託者および樹立者名を入れること。

Catalog # Resource name Availability Shipping form Fee (non-profit org.)
RDB02055 pT7/NS-CATc Under QC test. Please contact us. DNA solution JPY 9,460 (not-for-profit academic purpose)
plus cost of shipping containers, dry ice (if required) and shipping charge

check Please wait for results of QC test to be uploaded. This clone will be sequenced a portion for examination.

How to cite this biological resource

Materials & Methods section:

The pT7/NS-CATc was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB02055).

Reference section:

Nakagawa, Y., Oda, K., Nakada, S., The PB1 subunit alone can catalyze cRNA synthesis, and the PA subunit in addition to the PB1 subunit is required for viral RNA synthesis in replication of the influenza virus genome. J. Virol., 70, 6390-6394 (1996). PMID 8709268. [link to RRC of NBRP]
Nakagawa, Y., Kimura, N., Toyoda, T., Mizumoto, K., Ishihama, A., Oda, K., Nakada, S., The RNA polymerase PB2 subunit is not required for replication of the influenza virus genome but is involved in capped mRNA synthesis. J. Virol., 69, 727-733 (1995). PMID 7815536. [link to RRC of NBRP]
Kimura, N., Fukushima, A., Oda, K., Nakada, S., An in vivo study of the replication origin in the influenza virus complementary RNA. J. Biochem., 113, 88-92 (1993). PMID 7681056. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

check Please wait for results of QC test to be uploaded. This clone will be sequenced a portion for examination.

References

Original, user report and related articles

original Nakagawa, Y., The PB1 subunit alone can catalyze cRNA synthesis, and the PA subunit in addition to the PB1 subunit is required for viral RNA synthesis in replication of the influenza virus genome. J. Virol., 70, 6390-6394 (1996). PMID 8709268. [link to RRC of NBRP]
original Nakagawa, Y., The RNA polymerase PB2 subunit is not required for replication of the influenza virus genome but is involved in capped mRNA synthesis. J. Virol., 69, 727-733 (1995). PMID 7815536. [link to RRC of NBRP]
original Kimura, N., An in vivo study of the replication origin in the influenza virus complementary RNA. J. Biochem., 113, 88-92 (1993). PMID 7681056. [link to RRC of NBRP]