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pBMSA-NP (#RDB01972)

Influenza virus NP (influenza virus)

Clone info. Influenza virus (A/PR/8/34) NP gene inducible plasmid. Nucleotide sequence 1 to 1565nt of AF389119.1 (CDS, 46 to 1542nt) is cloned.
Comment Related cell lines RCB1506, RCB1508, RCB1509 are available from RIKEN Cell Bank. Known differences (at least): G to T substitution at 1527nt (Glu to Asp at 494aa).
Vector backbone pBMSA (Plasmid)
Size of vector backbone 13.2 kb
Selectable markers Amp^r
Gene/insert name Influenza virus NP cDNA
Depositor|Developer Nakada, Susumu |
 
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MTAに書く使用条件 研究結果の発表時、謝辞に寄託者および樹立者名を入れること。

Catalog # Resource name Shipping form Fee (non-profit org.)
RDB01972 pBMSA-NP DNA solution JPY 9,460 (not-for-profit academic purpose)
plus cost of shipping containers, dry ice (if required) and shipping charge

Please review the QC test results indicated by check icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The pBMSA-NP was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB01972).

Reference section:

Nakamura, Y., Oda, K., Nakada, S., Growth complementation of influenza virus temperature-sensitive mutants in mouse cells which express the RNA polymerase and nucleoprotein genes. J. Biochem., 110, 395-401 (1991). PMID 1663110. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB15079_A7Eop1-3.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: MMTV-LTR#2 (Pr0460)
Region: MMTV-LTR,insert 5'
Sequence file: RDB15079_A7Eoa.seq check
>01972_15079_A7Eo_5_MMLV-LTR#2_E05_14_ABI24.ab1
    1 CTGGGGTTGT GTCTGTTCGC CATCCCGTCT CCGCTCGTCA CTTATCCTTC ACTTTCCAGA
   61 GGGTCCCCCC GCAGACCCCG GCGACCCTCA GGTCGGCCGA CTGCGGCAGC TCTAGAGGAT
  121 CCCCGGGCGA GCTCGAATTC CAGCAAAAGC AGGGTAGATA ATCACTCACT GAGTGACATC
  181 AAAATCATGG CGTCCCAAGG CACCAAACGG TCTTACGAAC AGATGGAGAC TGATGGAGAA
  241 CGCCAGAATG CCACTGAAAT CAGAGCATCC GTCGGAAAAA TGATTGGTGG AATTGGACGA
  301 TTCTACATCC AAATGTGCAC CGAACTCAAA CTCAGTGATT ATGAGGGACG GTTGATCCAA
  361 AACAGCTTAA CAATAGAGAG AATGGTGCTC TCTGCTTTTG ACGAAAGGAG AAATAAATAC
  421 CTGGAAGAAC ATCCCAGTGC GGGGAAAGAT CCTAAGAAAA CTGGAGGACC TATATACAGG
  481 AGAGTAAACG GAAAGTGGAT GAGAGAACTC ATCCTTTATG ACAAAGAAGA AATAAGGCGA
  541 ATCTGGCGCC AAGCTAATAA TGGTGACGAT GCAACGGCTG GTCTGACTCA CATGATGATC
  601 TGGCATTCCA ATTTGAATGA TGCAACTTAT CAGAGGACAA GAGCTCTTGT TCGCACCGGA
  661 ATGGATCCCA GGATGTGCTC TCTGATGCAA GGTTCAACTC TCCCTAGGAG GTCTGGAGCC
  721 GCAGGTGCTG CAGTCAAAGG AGTTGGAACA ATGGTGATGG AATTGGTCAG GATGATCAAA
  781 CGTGGGATCA ATGATCGGAA CTTCTGGAGG GTGAGAATGG ACGAAAAACA AGAATTGCTT
  841 ATGAAAGAAT GTGCAACATT CTCAAGGGAA ATTTCAAACT GCTGCACAAA AAGCAATGAT
  901 GGATCAAGTG AGAGAGAGCC CGGAACCCAA GGGAATGCTG AGTTCGAGAT CTCACTTTTC
  961 TAGCACGGTC TGCACTCATA TTGGAGAGGG TCGGTGGCTC ACAGTCTGCT GCTGCCTGGT
 1021 GTGTATGACT GGCGTAGCAG TGGGTTACGA CTTGAAGAAG AGGGATACTC TAGTCGGATA
1081 GACCTTCCGA CTGCTTCCAA ACCAGC
//
Primer: SV40NLS_R (Pr0462)
Region: insert 3'
Sequence file: RDB15079_A7Eob.seq check
>01972_15079_A7Eo_5_SV40NLS#1_G05_20_ABI24.ab1
    1 GGTGGGGGTA GCAGTAGCCT CATCATCACT AGATGGCATT TCTTCTGAGC AAAACAGGTT
   61 TTCCTCATTA AAGGCATTCC ACCACTGCTC CCATTCATCA GTTCCATAGG TTGGAATCTA
  121 AAATACACAA ACAATTAGAA TCAGTAGTTT AACACATTAT ACACTTAAAA ATTTTATATT
  181 TACCTTAGAG CTTTAAATCT CTGTAGGTAG TTTGTCCAAT TATGTCACAC CACAGAAGTA
  241 AGGTTCCTTC ACAAAGATCC TCTAGAGGAT CCCCGGGCGA GCTCGAATTC CAGTAGAAAC
  301 AAGGGTATTT TTCTTTAATT GTCGTACTCA TCTGCATTGT CTCCGAAGAA ATAAGATCCT
  361 TCATTACTCA TGTCAAAGGA AGGCACGATC GGGCTCGCTG CCTTTTCGTC CGAGAGCTCG
  421 AAGACTCCCC GCCCCTGGAA AGACACATCT TCTGGTCTTG CACTTTCCAT CATCCTTATG
  481 ATTTCGGTCC TCATGTCAGA TGTTCTTCCC TCTGTATTCC CATTGAATGC TGCCATAATG
  541 GTTGTTCTGT CAAAAGGGAG ATTTCTCTGT ACTGAGAACG TAGGTTGTAT GCTGATTTGG
  601 CCCGCAGATG CCCTCTGTTG ATTGGTGTTT CCTCCACTTC TGGTCCTTAT GGCCCAGTAC
  661 CTGCTTCTCA GTTCAAGTGT ACTTGATTCC ATAGTCTCCA TATTTTCATT GGAAGCAATT
  721 TGAACTCCTC TAGTGGAAAG CTTCCCTCTT GGGAGCACCT TCGTCCCTTT GATGAAGCTT
  781 AATACTCTTA GATCTTCAAA TGCGGCAGAA TGGCATGCCA TCCACACCAG TTGACTCTTG
  841 TGTGCTGGAT TCTCATTTGG TCTGATTAGG CTGTACACTT GGCTGTTTTG AAGCAGTCTG
  901 AAAAGGGTCT ATTCCGACTA GAGAGTATCC CTCTTCTTTC AAAGTCGTAC CCACTGGCTA
  961 CGGCAGGTCC ATACACACAG GCAAGGCAGG CAGGACTTGT GAGCACCCGA CCCCTCCTCA
 1021 TATGAGTGCA GAACCGTGCT AGAAAAGTGA GATCTTTCGA CTCCAGCAAT TCCCTGGGGT
 1081 TCAGCTCCTC CTTCTCCACT TTGAATTCAA TCCATGGCAT TTTTGTGTGC AGCAGTGAAT
1141 TCT
//
Primer: pJB8-Forward (Pr0084)
Region: SV40pA,BPV-1 5'
Sequence file: RDB15079_A7Eoc.seq check
>01972_15079_A7Eo_pJB8-Forward_D01_04_ABI08.ab1
    1 TGAGTGGATC ATTCCCGGTC CGCCAGGGGG ACATGCCGGC GATGCTGAAG GTCGCGCGCA
   61 TTCCCGATGA AGAGGCCGGT TACCGCCTGT TGACCTGGTG GGACGGGCAG GGCGCCGCCC
  121 GAGTCTTCGC CTCGGCGGCG GGCGCTCTGC TCATGGAGCG CGCGTCCGGG GCCGGGGACC
  181 TTGCACAGAT AGCGTGGTCC GGCCAGGACG ACGAGGCTTG CAGGATCATA ATCAGCCATA
  241 CCACATTTGT AGAGGTTTTA CTTGCTTTAA AAAACCTCCC ACACCTCCCC CTGAACCTGA
  301 AACATAAAAT GAATGCAATT GTTGTTGTTA ACTTGTTTAT TGCAGCTTAT AATGGTTACA
  361 AATAAAGCAA TAGCATCACA AATTTCACAA ATAAAGCATT TTTTTCACTG CATTCTAGTT
  421 GTGGTTTGTC CAAACTCATC AATGTATCTT ATCATGTCTG GATCGATCCA GAGCTGTAAC
  481 AGCAGGGACC CGCCCCAGTA TAGGTGCGGG CATTCCTTTA GACACCCTTG AAACTCTTGG
  541 GGCCTTGCGT CCAGGGGTGT ATGAGGACAC TGTGCTACCA GAGGCCCCTG CAATAGTCAC
  601 TCCTGATGCT GTTCCTGCAG ATTCAGGGCT TGATGCCCTG TCCATAGGTA CAGACTCGTC
  661 CACGGAGACC CTCATTACTC TGCTAGAGCC TGAGGGTCCC GAGGACATAG CGGTTCTTGA
  721 GCTGCAACCC CTGGACCGTC CAACTTGGCA AGTAAGCAAT GCTGTTCATC AGTCCTCTGC
  781 ATACCACGCC CCCTCTGCAG CTGCAATCGT CCATTGCAGA AACATCTGGT TTAGAAAATA
  841 TTTTTGTAGG AGGCTCGGGT TTAGGGGATA CAGGAGGAGA AAACATTGAA CTGACATACT
  901 TCGGGTCCCC ACGAACAAGC ACGCCCCGCA GTATTGCCCT CTAAATCACG TGGCATTTTA
  961 AACTGGTTCA GTAAACGGTA CTACACACAA GGTGCCCACG AGATCTGGAG TGTTTTCAAT
 1021 CCTAACATTT GCCAAACCCC ACTGGTATGG AAGCGACCAG CCTGTGCCTA AGACTAGTGA
1081 CGTGTGAACT CAGTTCAGTT TATATAAAAC TG
//
Primer: BPV-1_F (Pr0458)
Region: BPV-1 3',SV40pA
Sequence file: RDB15079_A7Eod.seq check
>01972_15079_A7Eo_5_BPV-1_F_F05_17_ABI24.ab1
    1 CATGCATGGA TCTACTGGAG GGTTGCTGCA GGTGGATCAC CAAGGTACAC ACCACTCCGA
   61 ACAGCAGGGT CCACATCATC GCTTGCATCA ATAGGATCCA GACATGATAA GATACATTGA
  121 TGAGTTTGGA CAAACCACAA CTAGAATGCA GTGAAAAAAA TGCTTTATTT GTGAAATTTG
  181 TGATGCTATT GCTTTATTTG TAACCATTAT AAGCTGCAAT AAACAAGTTA ACAACAACAA
  241 TTGCATTCAT TTTATGTTTC AGGTTCAGGG GGAGGTGTGG GAGGTTTTTT AAAGCAAGTA
  301 AAACCTCTAC AAATGTGGTA TGGCTGATTA TGATCTCTAG TCAAGGCACT ATACATCAAA
  361 TATTCCTTAT TAACCCCTTT ACAAATTAAA AAGCTAAAGG TACACAATTT TTGAGCATAG
  421 TTATTAATAG CAGACACTCT ATGCCTGTGT GGAGTAAGAA AAAACAGTAT GTTATGATTA
  481 TAACTGTTAT GCCTACTTAT AAAGGTTACA GAATATTTTT CCATAATTTT CTTGTATAGC
  541 AGTGCAGCTT TTTCCTTTGT GGTGTAAATA GCAAAGCAAG CAAGAGTTCT ATTACTAAAC
  601 ACAGCATGAC TCAAAAAACT TAGCAATTCT GAAGGAAAGT CCTTGGGGTC TTCTACCTTT
  661 CTCTTCTTTT TTGGAGGAGT AGAATGTTGA GAGTCAGCAG TAGCCTCATC ATCACTAGAT
  721 GGCATTTCTT CTGAGCAAAA CAGGTTTTCC TCATTAAAGG CATTCCACCA CTGCTCCCAT
  781 TCATCAGTTC CATAGGTTGG AATCTAAAAT ACACAAACAA TTAGAATCAG TAGTTTAACA
  841 CATTATACAC TTAAAAATTT TATATTTACC TTAGAGCTTT AAATCTCTGT AGGTAGTTTG
  901 TCCAATTATG TCACACCACA GAAGTAAGGT TTCCTTTCAC AAAAGATCCC TTCTAGAAGG
961 GATCCCCCG
//

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

original Nakamura, Y., Growth complementation of influenza virus temperature-sensitive mutants in mouse cells which express the RNA polymerase and nucleoprotein genes. J. Biochem., 110, 395-401 (1991). PMID 1663110. [link to RRC of NBRP]
user_report Buriani, G., Heat-shock protein 70 from plant biofactories of recombinant antigens activate multiepitope-targeted immune responses. Plant Biotechnol J., 10 (3): 363-371 (2012). PMID 22221920. [link to RRC of NBRP]