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pBMSA-NP

Influenza virus NP (influenza virus)

Catalog number RDB01972
Resource name pBMSA-NP
Clone info. Influenza virus (A/PR/8/34) NP gene inducible plasmid. Nucleotide sequence 1 to 1565nt of AF389119.1 (CDS, 46 to 1542nt) is cloned.
Comment Related cell lines RCB1506, RCB1508, RCB1509 are available from RIKEN Cell Bank. Known differences (at least): G to T substitution at 1527nt (Glu to Asp at 494aa).
Vector backbone pBMSA (Plasmid)
Size of vector backbone 13.2 kb
Selectable markers Amp^r
Gene/insert name Influenza virus NP cDNA
Depositor|Developer Nakada, Susumu |
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External Database
Influenza virus NP

          Reference sequence
            
           
          Remarks, protocol and/or map (gif) RDB01972.gif

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          RDB01972 pBMSA-NP DNA solution

          Please review the QC test results indicated by check icon below as well as clone information before placing your order.

          How to cite this biological resource

          Materials & Methods section:

          The pBMSA-NP was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB01972).

          Reference section:

          Nakamura, Y., Oda, K., Nakada, S., Growth complementation of influenza virus temperature-sensitive mutants in mouse cells which express the RNA polymerase and nucleoprotein genes. J. Biochem., 110, 395-401 (1991). PMID 1663110.

          Further references such as user reports and related articles (go to bottom)


          References and tips

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          QC test results

          RIKEN BRC has sequenced portions of this material for quality test.
          Please review the QC test results indicated by check icon as well as clone information before placing your order.

          Test sheet RDB15079_A7Eop1-3.pdf check

          Nucleotide sequence of a portion of this resource (if available).

          Primer: MMTV-LTR#2 (Pr0460)
          Region: MMTV-LTR,insert 5'
          Sequence file: RDB15079_A7Eoa.seq check
          >01972_15079_A7Eo_5_MMLV-LTR#2_E05_14_ABI24.ab1
              1 CTGGGGTTGT GTCTGTTCGC CATCCCGTCT CCGCTCGTCA CTTATCCTTC ACTTTCCAGA
             61 GGGTCCCCCC GCAGACCCCG GCGACCCTCA GGTCGGCCGA CTGCGGCAGC TCTAGAGGAT
            121 CCCCGGGCGA GCTCGAATTC CAGCAAAAGC AGGGTAGATA ATCACTCACT GAGTGACATC
            181 AAAATCATGG CGTCCCAAGG CACCAAACGG TCTTACGAAC AGATGGAGAC TGATGGAGAA
            241 CGCCAGAATG CCACTGAAAT CAGAGCATCC GTCGGAAAAA TGATTGGTGG AATTGGACGA
            301 TTCTACATCC AAATGTGCAC CGAACTCAAA CTCAGTGATT ATGAGGGACG GTTGATCCAA
            361 AACAGCTTAA CAATAGAGAG AATGGTGCTC TCTGCTTTTG ACGAAAGGAG AAATAAATAC
            421 CTGGAAGAAC ATCCCAGTGC GGGGAAAGAT CCTAAGAAAA CTGGAGGACC TATATACAGG
            481 AGAGTAAACG GAAAGTGGAT GAGAGAACTC ATCCTTTATG ACAAAGAAGA AATAAGGCGA
            541 ATCTGGCGCC AAGCTAATAA TGGTGACGAT GCAACGGCTG GTCTGACTCA CATGATGATC
            601 TGGCATTCCA ATTTGAATGA TGCAACTTAT CAGAGGACAA GAGCTCTTGT TCGCACCGGA
            661 ATGGATCCCA GGATGTGCTC TCTGATGCAA GGTTCAACTC TCCCTAGGAG GTCTGGAGCC
            721 GCAGGTGCTG CAGTCAAAGG AGTTGGAACA ATGGTGATGG AATTGGTCAG GATGATCAAA
            781 CGTGGGATCA ATGATCGGAA CTTCTGGAGG GTGAGAATGG ACGAAAAACA AGAATTGCTT
            841 ATGAAAGAAT GTGCAACATT CTCAAGGGAA ATTTCAAACT GCTGCACAAA AAGCAATGAT
            901 GGATCAAGTG AGAGAGAGCC CGGAACCCAA GGGAATGCTG AGTTCGAGAT CTCACTTTTC
            961 TAGCACGGTC TGCACTCATA TTGGAGAGGG TCGGTGGCTC ACAGTCTGCT GCTGCCTGGT
           1021 GTGTATGACT GGCGTAGCAG TGGGTTACGA CTTGAAGAAG AGGGATACTC TAGTCGGATA
          1081 GACCTTCCGA CTGCTTCCAA ACCAGC
          //
          Primer: SV40NLS_R (Pr0462)
          Region: insert 3'
          Sequence file: RDB15079_A7Eob.seq check
          >01972_15079_A7Eo_5_SV40NLS#1_G05_20_ABI24.ab1
              1 GGTGGGGGTA GCAGTAGCCT CATCATCACT AGATGGCATT TCTTCTGAGC AAAACAGGTT
             61 TTCCTCATTA AAGGCATTCC ACCACTGCTC CCATTCATCA GTTCCATAGG TTGGAATCTA
            121 AAATACACAA ACAATTAGAA TCAGTAGTTT AACACATTAT ACACTTAAAA ATTTTATATT
            181 TACCTTAGAG CTTTAAATCT CTGTAGGTAG TTTGTCCAAT TATGTCACAC CACAGAAGTA
            241 AGGTTCCTTC ACAAAGATCC TCTAGAGGAT CCCCGGGCGA GCTCGAATTC CAGTAGAAAC
            301 AAGGGTATTT TTCTTTAATT GTCGTACTCA TCTGCATTGT CTCCGAAGAA ATAAGATCCT
            361 TCATTACTCA TGTCAAAGGA AGGCACGATC GGGCTCGCTG CCTTTTCGTC CGAGAGCTCG
            421 AAGACTCCCC GCCCCTGGAA AGACACATCT TCTGGTCTTG CACTTTCCAT CATCCTTATG
            481 ATTTCGGTCC TCATGTCAGA TGTTCTTCCC TCTGTATTCC CATTGAATGC TGCCATAATG
            541 GTTGTTCTGT CAAAAGGGAG ATTTCTCTGT ACTGAGAACG TAGGTTGTAT GCTGATTTGG
            601 CCCGCAGATG CCCTCTGTTG ATTGGTGTTT CCTCCACTTC TGGTCCTTAT GGCCCAGTAC
            661 CTGCTTCTCA GTTCAAGTGT ACTTGATTCC ATAGTCTCCA TATTTTCATT GGAAGCAATT
            721 TGAACTCCTC TAGTGGAAAG CTTCCCTCTT GGGAGCACCT TCGTCCCTTT GATGAAGCTT
            781 AATACTCTTA GATCTTCAAA TGCGGCAGAA TGGCATGCCA TCCACACCAG TTGACTCTTG
            841 TGTGCTGGAT TCTCATTTGG TCTGATTAGG CTGTACACTT GGCTGTTTTG AAGCAGTCTG
            901 AAAAGGGTCT ATTCCGACTA GAGAGTATCC CTCTTCTTTC AAAGTCGTAC CCACTGGCTA
            961 CGGCAGGTCC ATACACACAG GCAAGGCAGG CAGGACTTGT GAGCACCCGA CCCCTCCTCA
           1021 TATGAGTGCA GAACCGTGCT AGAAAAGTGA GATCTTTCGA CTCCAGCAAT TCCCTGGGGT
           1081 TCAGCTCCTC CTTCTCCACT TTGAATTCAA TCCATGGCAT TTTTGTGTGC AGCAGTGAAT
          1141 TCT
          //
          Primer: pJB8-Forward (Pr0084)
          Region: SV40pA,BPV-1 5'
          Sequence file: RDB15079_A7Eoc.seq check
          >01972_15079_A7Eo_pJB8-Forward_D01_04_ABI08.ab1
              1 TGAGTGGATC ATTCCCGGTC CGCCAGGGGG ACATGCCGGC GATGCTGAAG GTCGCGCGCA
             61 TTCCCGATGA AGAGGCCGGT TACCGCCTGT TGACCTGGTG GGACGGGCAG GGCGCCGCCC
            121 GAGTCTTCGC CTCGGCGGCG GGCGCTCTGC TCATGGAGCG CGCGTCCGGG GCCGGGGACC
            181 TTGCACAGAT AGCGTGGTCC GGCCAGGACG ACGAGGCTTG CAGGATCATA ATCAGCCATA
            241 CCACATTTGT AGAGGTTTTA CTTGCTTTAA AAAACCTCCC ACACCTCCCC CTGAACCTGA
            301 AACATAAAAT GAATGCAATT GTTGTTGTTA ACTTGTTTAT TGCAGCTTAT AATGGTTACA
            361 AATAAAGCAA TAGCATCACA AATTTCACAA ATAAAGCATT TTTTTCACTG CATTCTAGTT
            421 GTGGTTTGTC CAAACTCATC AATGTATCTT ATCATGTCTG GATCGATCCA GAGCTGTAAC
            481 AGCAGGGACC CGCCCCAGTA TAGGTGCGGG CATTCCTTTA GACACCCTTG AAACTCTTGG
            541 GGCCTTGCGT CCAGGGGTGT ATGAGGACAC TGTGCTACCA GAGGCCCCTG CAATAGTCAC
            601 TCCTGATGCT GTTCCTGCAG ATTCAGGGCT TGATGCCCTG TCCATAGGTA CAGACTCGTC
            661 CACGGAGACC CTCATTACTC TGCTAGAGCC TGAGGGTCCC GAGGACATAG CGGTTCTTGA
            721 GCTGCAACCC CTGGACCGTC CAACTTGGCA AGTAAGCAAT GCTGTTCATC AGTCCTCTGC
            781 ATACCACGCC CCCTCTGCAG CTGCAATCGT CCATTGCAGA AACATCTGGT TTAGAAAATA
            841 TTTTTGTAGG AGGCTCGGGT TTAGGGGATA CAGGAGGAGA AAACATTGAA CTGACATACT
            901 TCGGGTCCCC ACGAACAAGC ACGCCCCGCA GTATTGCCCT CTAAATCACG TGGCATTTTA
            961 AACTGGTTCA GTAAACGGTA CTACACACAA GGTGCCCACG AGATCTGGAG TGTTTTCAAT
           1021 CCTAACATTT GCCAAACCCC ACTGGTATGG AAGCGACCAG CCTGTGCCTA AGACTAGTGA
          1081 CGTGTGAACT CAGTTCAGTT TATATAAAAC TG
          //
          Primer: BPV-1_F (Pr0458)
          Region: BPV-1 3',SV40pA
          Sequence file: RDB15079_A7Eod.seq check
          >01972_15079_A7Eo_5_BPV-1_F_F05_17_ABI24.ab1
              1 CATGCATGGA TCTACTGGAG GGTTGCTGCA GGTGGATCAC CAAGGTACAC ACCACTCCGA
             61 ACAGCAGGGT CCACATCATC GCTTGCATCA ATAGGATCCA GACATGATAA GATACATTGA
            121 TGAGTTTGGA CAAACCACAA CTAGAATGCA GTGAAAAAAA TGCTTTATTT GTGAAATTTG
            181 TGATGCTATT GCTTTATTTG TAACCATTAT AAGCTGCAAT AAACAAGTTA ACAACAACAA
            241 TTGCATTCAT TTTATGTTTC AGGTTCAGGG GGAGGTGTGG GAGGTTTTTT AAAGCAAGTA
            301 AAACCTCTAC AAATGTGGTA TGGCTGATTA TGATCTCTAG TCAAGGCACT ATACATCAAA
            361 TATTCCTTAT TAACCCCTTT ACAAATTAAA AAGCTAAAGG TACACAATTT TTGAGCATAG
            421 TTATTAATAG CAGACACTCT ATGCCTGTGT GGAGTAAGAA AAAACAGTAT GTTATGATTA
            481 TAACTGTTAT GCCTACTTAT AAAGGTTACA GAATATTTTT CCATAATTTT CTTGTATAGC
            541 AGTGCAGCTT TTTCCTTTGT GGTGTAAATA GCAAAGCAAG CAAGAGTTCT ATTACTAAAC
            601 ACAGCATGAC TCAAAAAACT TAGCAATTCT GAAGGAAAGT CCTTGGGGTC TTCTACCTTT
            661 CTCTTCTTTT TTGGAGGAGT AGAATGTTGA GAGTCAGCAG TAGCCTCATC ATCACTAGAT
            721 GGCATTTCTT CTGAGCAAAA CAGGTTTTCC TCATTAAAGG CATTCCACCA CTGCTCCCAT
            781 TCATCAGTTC CATAGGTTGG AATCTAAAAT ACACAAACAA TTAGAATCAG TAGTTTAACA
            841 CATTATACAC TTAAAAATTT TATATTTACC TTAGAGCTTT AAATCTCTGT AGGTAGTTTG
            901 TCCAATTATG TCACACCACA GAAGTAAGGT TTCCTTTCAC AAAAGATCCC TTCTAGAAGG
          961 GATCCCCCG
          //

          Please visit Sequencing and PCR primers for primer information.


          References

          Original, user report and related articles

          original Nakamura, Y., Growth complementation of influenza virus temperature-sensitive mutants in mouse cells which express the RNA polymerase and nucleoprotein genes. J. Biochem., 110, 395-401 (1991). PMID 1663110.
          user_report Buriani, G., Heat-shock protein 70 from plant biofactories of recombinant antigens activate multiepitope-targeted immune responses. Plant Biotechnol J., 10 (3): 363-371 (2012). PMID 22221920.

          2023.05.02

          GNP_filter3_RDBDEP_html_230501.pl