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pBMSA-PA

Influenza virus RNA polymerase PA subunit gene

Catalog number RDB01971
Resource name pBMSA-PA
Clone info. Influenza virus (A/PR/8/34) RNA polymerase PA gene inducible plasmid. Nucleotide sequence 5 to 2233nt of AF389117.1 (CDS, 25 to 2175nt) is cloned.
Comment Related cell lines RCB1508, RCB1509 are available from RIKEN Cell Bank.
Vector backbone pBMSA (Plasmid)
Size of vector backbone 13.2 kb
Selectable markers Amp^r
Gene/insert name Influenza virus RNA polymerase PA cDNA
Depositor|Developer Nakada, Susumu |
Other clones in our bank
External Database
Influenza virus RNA polymerase PA
          Reference sequence
            
           
          Remarks, protocol and/or map (gif) RDB01971.gif

          Distribution information

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          RDB01971 pBMSA-PA DNA solution

          Please review the QC test results indicated by check icon below as well as clone information before placing your order.

          How to cite this biological resource

          Materials & Methods section:

          The pBMSA-PA was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB01971).

          Reference section:

          Nakamura, Y., Oda, K., Nakada, S., Growth complementation of influenza virus temperature-sensitive mutants in mouse cells which express the RNA polymerase and nucleoprotein genes. J. Biochem., 110, 395-401 (1991). PMID 1663110.

          Further references such as user reports and related articles (go to bottom)

          References and tips

          Featured content

          QC test results

          RIKEN BRC has sequenced portions of this material for quality test.
          Please review the QC test results indicated by check icon as well as clone information before placing your order.

          Test sheet RDB15078_A7Eop1-2.pdf check

          Nucleotide sequence of a portion of this resource (if available).

          Primer: MMTV-LTR#2 ()
          Sequence file: RDB15078_A7Eoa.seq check
          >01971_15078_A7Eo_MMLV-LTR#2_A05_02_ABI24.ab1
              1 GTGGGTTTGT GGTCTGTTCG CCATCCCGTC TCCGCTCGTC ACTTATCCTT CACTTTCCAG
             61 AGGGTCCCCC CGCAGACCCC GGCGACCCTC AGGTCGGCCG ACTGCGGCAG CTCTAGAGGA
            121 TCCCCGGGCG AGCTCGAATT CCAGCAAAAG CAGGTACTGA TCCAAAATGG AAGATTTTGT
            181 GCGACAATGC TTCAATCCGA TGATTGTCGA GCTTGCGGAA AAAACAATGA AAGAGTATGG
            241 GGAGGACCTG AAAATCGAAA CAAACAAATT TGCAGCAATA TGCACTCACT TGGAAGTATG
            301 CTTCATGTAT TCAGATTTTC ACTTCATCAA TGAGCAAGGC GAGTCAATAA TCGTAGAACT
            361 TGGTGATCCA AATGCACTTT TGAAGCACAG ATTTGAAATA ATCGAGGGAA GAGATCGCAC
            421 AATGGCCTGG ACAGTAGTAA ACAGTATTTG CAACACTACA GGGGCTGAGA AACCAAAGTT
            481 TCTACCAGAT TTGTATGATT ACAAGGAGAA TAGATTCATC GAAATTGGAG TAACAAGGAG
            541 AGAAGTTCAC ATATACTATC TGGAAAAGGC CAATAAAATT AAATCTGAGA AAACACACAT
            601 CCACATTTTC TCGTTCACTG GGGAAGAAAT GGCCACAAAG GCAGACTACA CTCTCGATGA
            661 AGAAAGCAGG GCTAGGATCA AAACCAGACT ATTCACCATA AGACAAGAAA TGGCCAGCAG
            721 AGGCCTCTGG GATTCCTTTC GTCAGTCCGA GAGAGGAGAA GAGACAATTG AAGAAAGGTT
            781 TGAAATCACA GGAACAATGC GCAAGCTTGC CGACCAAAGT CTCCCGCCGA ACTTCTCCAG
            841 CCTTGAAAAT TTTAGAGCCT ATGTGGATGG ATTCGAACCG AACGGCTACA TTGAGGGCAA
            901 GCTGTCTCAA ATGTCCAAAG AAGTAAATGC TAGAATTTGA ACCCTTTTTT GAAAACAACA
            961 CCACGACCAC TTAGACTTCC CGAATGGGCC TCCCTGTTCT CAGCGGGTCA AATCCTGCTG
           1021 GATGGATGCC CTTAAATAGC ATGAGACCAA GTCATGCAGA AGGATACGCT ATATTGATGC
          1081 ATCAATGCAT GACACCATTC CTTTGATGGG AAGGAAACCC
          //
          Primer: SV40NLS_R ()
          Sequence file: RDB15078_A7Eob.seq check
          >01971_15078_A7Eo_SV40NLS#1_C05_08_ABI24.ab1
              1 GGAGGGGGTA GCAGTAGCCT CATCATCACT AGATGGCATT TCTTCTGAGC AAAACAGGTT
             61 TTCCTCATTA AAGGCATTCC ACCACTGCTC CCATTCATCA GTTCCATAGG TTGGAATCTA
            121 AAATACACAA ACAATTAGAA TCAGTAGTTT AACACATTAT ACACTTAAAA ATTTTATATT
            181 TACCTTAGAG CTTTAAATCT CTGTAGGTAG TTTGTCCAAT TATGTCACAC CACAGAAGTA
            241 AGGTTCCTTC ACAAAGATCC TCTAGAGGAT CCCCGGGCGA GCTCGAATTC CAGTAGAAAC
            301 AAGGTACTTT TTTGGACAGT ATGGATAGCA AATAGTAGCA CTGCCACAAC TAACTCAATG
            361 CATGTGTAAG GAAGGAGTTG AACCAAGAAG CATTAAGCAA AACCCAGGGA TCATTAATCA
            421 GGCACTCCTC AATTGCTTCA TATAGCCCCC CAAGATCAAA GGTCCCAGGT TCAAGGTTGT
            481 CCCTAAGAGC CTGAACGATA AGAAGCAGTT TTCTTGATTC AGCTGAAAAT CCTTCTAGTT
            541 GTGGAGATGC ATACAAGCTG TTGAATACCG ACTTTGCTAA TAAAGTCCTG CAGACCTTCC
            601 CAATGGAACT TTCCTCCACT CCTTTGGGGG ACTCTCCAAT GGGCCATGTT TCTGATTTGT
            661 TCTCAAAGAA CTCTTTGGTC ATGTCTTTCT CTTTGACAGA GGACTCAGCT TCAATCATAC
            721 TCTCAATTTG TTGAAGTGAC TGGAGGAGGC AACGCCTCAT CTCCATTCCC CATTTCATTT
            781 TAATTTTTGA GGTTCCATTT GTTCTCACAT ACAAGAACAT GGGCCTTGAA ACCTGGCCCT
            841 ATGGCACTTC TTATAAGCAT ATCTCCTATC TCAAGAACAC AGTACTTCTC CCATTTATGT
            901 GGTTCAAGTC TTGGGTCAGT GAGAGAAAAC TCCATGCTCA CAAAGTTTAC CACGTCGTGT
            961 CATTCCTTAA GTGGGATCTT CCTTTTTATG ATGAACCATA CAGTGGGTCT TTCGCCTTCC
           1021 CTCCTAAGTC TACACTGCTA TCATGGATTA TGAATCATTC ATGCTCGCCA GATGCATTAA
          1081 GCAAGGCAGT ATGATGTTAA AACCCCCCGT TCAGA
          //
          Primer: pJB8-Forward ()
          Sequence file: RDB15078_A7Eoc.seq check
          >01971_15078_A7Eo_pJB8-Forward_E10_13_ABI24.ab1
              1 TTGACGGGGA ACTTTCCCGT CCGCCAGGGG GACATGCCGG CGATGCTGAA GGTCGCGCGC
             61 ATTCCCGATG AAGAGGCCGG TTACCGCCTG TTGACCTGGT GGGACGGGCA GGGCGCCGCC
            121 CGAGTCTTCG CCTCGGCGGC GGGCGCTCTG CTCATGGAGC GCGCGTCCGG GGCCGGGGAC
            181 CTTGCACAGA TAGCGTGGTC CGGCCAGGAC GACGAGGCTT GCAGGATCAT AATCAGCCAT
            241 ACCACATTTG TAGAGGTTTT ACTTGCTTTA AAAAACCTCC CACACCTCCC CCTGAACCTG
            301 AAACATAAAA TGAATGCAAT TGTTGTTGTT AACTTGTTTA TTGCAGCTTA TAATGGTTAC
            361 AAATAAAGCA ATAGCATCAC AAATTTCACA AATAAAGCAT TTTTTTCACT GCATTCTAGT
            421 TGTGGTTTGT CCAAACTCAT CAATGTATCT TATCATGTCT GGATCGATCC AGAGCTGTAA
            481 CAGCAGGGAC CCGCCCCAGT ATAGGTGCGG GCATTCCTTT AGACACCCTT GAAACTCTTG
            541 GGGCCTTGCG TCCAGGGGTG TATGAGGACA CTGTGCTACC AGAGGCCCCT GCAATAGTCA
            601 CTCCTGATGC TGTTCCTGCA GATTCAGGGC TTGATGCCCT GTCCATAGGT ACAGACTCGT
            661 CCACGGAGAC CCTCATTACT CTGCTAGAGC CTGAGGGTCC CGAGGACATA GCGGTTCTTG
            721 AGCTGCAACC CCTGGACCGT CCAACTTGGC AAGTAAGCAA TGCTGTTCAT CAGTCCTCTG
            781 CATACCACGC CCCTCTGCAG CTGCAATCGT CCATTGCAGA AACATCTGGT TTAGAAAAAT
            841 ATTTTTGTAG GAGGCTCGGG TTTAGGGGAT ACAGGAGGAG AAAACATTGA ACTGACATAC
            901 TTCGGGTCCC CACGAACAAG CACGCCCCGC AGTATTGCCT CTAAATCACG TGGCATTTTA
            961 AACTGGTTCA GTAAACGGTA CTACACACAG GTGCCCACGG AAGATCCTGA AGTGTTTTCA
           1021 TCCCAAACAT TGCAAACCCA CTGTATGAAG CAGACCCAGC TGTGCTTAGG ACCTAGTGAC
           1081 GTGTTGACTC AGTCAGTTTT ATAACCCTGA ATACACTACA CACGTAACCG GACAGAGTGG
          1141 ACCCACAAGC CTACCATGTC CAAGTAACTC CTCTCAATG
          //
          Primer: BPV-1_F ()
          Sequence file: RDB15078_A7Eod.seq check
          >01971_15078_A7Eo_BPV-1_F_B05_05_ABI24.ab1
              1 CATGGAATGG TCTCTGGAGG GTTGCTGCAG GTGGATCACC AAGGTACACA CCACTCCGAA
             61 CAGCAGGGTC CACATCATCG CTTGCATCAA TAGGATCCAG ACATGATAAG ATACATTGAT
            121 GAGTTTGGAC AAACCACAAC TAGAATGCAG TGAAAAAAAT GCTTTATTTG TGAAATTTGT
            181 GATGCTATTG CTTTATTTGT AACCATTATA AGCTGCAATA AACAAGTTAA CAACAACAAT
            241 TGCATTCATT TTATGTTTCA GGTTCAGGGG GAGGTGTGGG AGGTTTTTTA AAGCAAGTAA
            301 AACCTCTACA AATGTGGTAT GGCTGATTAT GATCTCTAGT CAAGGCACTA TACATCAAAT
            361 ATTCCTTATT AACCCCTTTA CAAATTAAAA AGCTAAAGGT ACACAATTTT TGAGCATAGT
            421 TATTAATAGC AGACACTCTA TGCCTGTGTG GAGTAAGAAA AAACAGTATG TTATGATTAT
            481 AACTGTTATG CCTACTTATA AAGGTTACAG AATATTTTTC CATAATTTTC TTGTATAGCA
            541 GTGCAGCTTT TTCCTTTGTG GTGTAAATAG CAAAGCAAGC AAGAGTTCTA TTACTAAACA
            601 CAGCATGACT CAAAAAACTT AGCAATTCTG AAGGAAAGTC CTTGGGGTCT TCTACCTTTC
            661 TCTTCTTTTT TGGAGGAGTA GAATGTTGAG AGTCAGCAGT AGCCTCATCA TCACTAGATG
            721 GCATTTCTTC TGAGCAAAAC AGGTTTTCCT CATTAAAGGC ATTCCACCAC TGCTCCCATT
            781 CATCAGTTCC ATAGGTTGGA ATCTAAAATA CACAAACAAT TAGAATCAGT AGTTTAACAC
            841 ATTATACACT TAAAAATTTT ATATTTACCT TAGAGCTTTA AATCTCTGTA GTAGTTTGTC
            901 CAATTTATGT CACACCACAG AAAGTAAGGT TCCTTCACAA AGATCCTTCT AGAAGGATCC
          961 CCGGG
          //

          Please visit Sequencing and PCR primers for primer information.

          References

          Original, user report and related articles

          original Nakamura, Y., Growth complementation of influenza virus temperature-sensitive mutants in mouse cells which express the RNA polymerase and nucleoprotein genes. J. Biochem., 110, 395-401 (1991). PMID 1663110.
          user_report Kuzuhara, T., Preparation of Recombinant PA Endonuclease Domain Protein of Influenza A Virus and Its Application for Glycobiology Research. Methods Mol. Biol. 2556: 69-78 (2022). PMID 36175628.
          user_report Shirayama, R., Inhibition of PA endonuclease activity of influenza virus RNA polymerase by Kampo medicines. Drug Discov. Ther. 10 (2): 109-113 (2016). PMID 26902927.
          user_report Shoji, M., Anti-influenza activity of c60 fullerene derivatives. PLoS One, 8 (6): e66337 (2013). PMID 23785493.
          user_report Iwai, Y., Anti-influenza activity of marchantins, macrocyclic bisbibenzyls contained in liverworts. PLoS One 6 (5): e19825 (2011). PMID 21625478.
          user_report Iwai Y., Anti-influenza activity of phenethylphenylphthalimide analogs derived from thalidomide. Bioorg. Med. Chem., 18, 5379-5390 (2010). PMID 20538468.
          user_report Kuzuhara T., Green tea catechins inhibit the endonuclease activity of influenza A virus RNA polymerase. PLoS Curr., 1, RRN1052 (2009). PMID 20025206.

          2023.05.22

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