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pBMSA-PA (#RDB01971)

Influenza virus RNA polymerase PA subunit gene

Clone info. Influenza virus (A/PR/8/34) RNA polymerase PA gene inducible plasmid. Nucleotide sequence 5 to 2233nt of AF389117.1 (CDS, 25 to 2175nt) is cloned.
Comment Related cell lines RCB1508, RCB1509 are available from RIKEN Cell Bank.
Vector backbone pBMSA (Plasmid)
Size of vector backbone 13.2 kb
Selectable markers Amp^r
Gene/insert name Influenza virus RNA polymerase PA cDNA
Depositor|Developer Nakada, Susumu |
 
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Distribution information

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MTAに書く使用条件 研究結果の発表時、謝辞に寄託者および樹立者名を入れること。

Catalog # Resource name Shipping form Fee (non-profit org.)
RDB01971 pBMSA-PA DNA solution JPY 9,460 (not-for-profit academic purpose)
plus cost of shipping containers, dry ice (if required) and shipping charge

Please review the QC test results indicated by check icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The pBMSA-PA was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB01971).

Reference section:

Nakamura, Y., Oda, K., Nakada, S., Growth complementation of influenza virus temperature-sensitive mutants in mouse cells which express the RNA polymerase and nucleoprotein genes. J. Biochem., 110, 395-401 (1991). PMID 1663110. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB15078_A7Eop1-2.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: MMTV-LTR#2 ()
Sequence file: RDB15078_A7Eoa.seq check
>01971_15078_A7Eo_MMLV-LTR#2_A05_02_ABI24.ab1
    1 GTGGGTTTGT GGTCTGTTCG CCATCCCGTC TCCGCTCGTC ACTTATCCTT CACTTTCCAG
   61 AGGGTCCCCC CGCAGACCCC GGCGACCCTC AGGTCGGCCG ACTGCGGCAG CTCTAGAGGA
  121 TCCCCGGGCG AGCTCGAATT CCAGCAAAAG CAGGTACTGA TCCAAAATGG AAGATTTTGT
  181 GCGACAATGC TTCAATCCGA TGATTGTCGA GCTTGCGGAA AAAACAATGA AAGAGTATGG
  241 GGAGGACCTG AAAATCGAAA CAAACAAATT TGCAGCAATA TGCACTCACT TGGAAGTATG
  301 CTTCATGTAT TCAGATTTTC ACTTCATCAA TGAGCAAGGC GAGTCAATAA TCGTAGAACT
  361 TGGTGATCCA AATGCACTTT TGAAGCACAG ATTTGAAATA ATCGAGGGAA GAGATCGCAC
  421 AATGGCCTGG ACAGTAGTAA ACAGTATTTG CAACACTACA GGGGCTGAGA AACCAAAGTT
  481 TCTACCAGAT TTGTATGATT ACAAGGAGAA TAGATTCATC GAAATTGGAG TAACAAGGAG
  541 AGAAGTTCAC ATATACTATC TGGAAAAGGC CAATAAAATT AAATCTGAGA AAACACACAT
  601 CCACATTTTC TCGTTCACTG GGGAAGAAAT GGCCACAAAG GCAGACTACA CTCTCGATGA
  661 AGAAAGCAGG GCTAGGATCA AAACCAGACT ATTCACCATA AGACAAGAAA TGGCCAGCAG
  721 AGGCCTCTGG GATTCCTTTC GTCAGTCCGA GAGAGGAGAA GAGACAATTG AAGAAAGGTT
  781 TGAAATCACA GGAACAATGC GCAAGCTTGC CGACCAAAGT CTCCCGCCGA ACTTCTCCAG
  841 CCTTGAAAAT TTTAGAGCCT ATGTGGATGG ATTCGAACCG AACGGCTACA TTGAGGGCAA
  901 GCTGTCTCAA ATGTCCAAAG AAGTAAATGC TAGAATTTGA ACCCTTTTTT GAAAACAACA
  961 CCACGACCAC TTAGACTTCC CGAATGGGCC TCCCTGTTCT CAGCGGGTCA AATCCTGCTG
 1021 GATGGATGCC CTTAAATAGC ATGAGACCAA GTCATGCAGA AGGATACGCT ATATTGATGC
1081 ATCAATGCAT GACACCATTC CTTTGATGGG AAGGAAACCC
//
Primer: SV40NLS_R ()
Sequence file: RDB15078_A7Eob.seq check
>01971_15078_A7Eo_SV40NLS#1_C05_08_ABI24.ab1
    1 GGAGGGGGTA GCAGTAGCCT CATCATCACT AGATGGCATT TCTTCTGAGC AAAACAGGTT
   61 TTCCTCATTA AAGGCATTCC ACCACTGCTC CCATTCATCA GTTCCATAGG TTGGAATCTA
  121 AAATACACAA ACAATTAGAA TCAGTAGTTT AACACATTAT ACACTTAAAA ATTTTATATT
  181 TACCTTAGAG CTTTAAATCT CTGTAGGTAG TTTGTCCAAT TATGTCACAC CACAGAAGTA
  241 AGGTTCCTTC ACAAAGATCC TCTAGAGGAT CCCCGGGCGA GCTCGAATTC CAGTAGAAAC
  301 AAGGTACTTT TTTGGACAGT ATGGATAGCA AATAGTAGCA CTGCCACAAC TAACTCAATG
  361 CATGTGTAAG GAAGGAGTTG AACCAAGAAG CATTAAGCAA AACCCAGGGA TCATTAATCA
  421 GGCACTCCTC AATTGCTTCA TATAGCCCCC CAAGATCAAA GGTCCCAGGT TCAAGGTTGT
  481 CCCTAAGAGC CTGAACGATA AGAAGCAGTT TTCTTGATTC AGCTGAAAAT CCTTCTAGTT
  541 GTGGAGATGC ATACAAGCTG TTGAATACCG ACTTTGCTAA TAAAGTCCTG CAGACCTTCC
  601 CAATGGAACT TTCCTCCACT CCTTTGGGGG ACTCTCCAAT GGGCCATGTT TCTGATTTGT
  661 TCTCAAAGAA CTCTTTGGTC ATGTCTTTCT CTTTGACAGA GGACTCAGCT TCAATCATAC
  721 TCTCAATTTG TTGAAGTGAC TGGAGGAGGC AACGCCTCAT CTCCATTCCC CATTTCATTT
  781 TAATTTTTGA GGTTCCATTT GTTCTCACAT ACAAGAACAT GGGCCTTGAA ACCTGGCCCT
  841 ATGGCACTTC TTATAAGCAT ATCTCCTATC TCAAGAACAC AGTACTTCTC CCATTTATGT
  901 GGTTCAAGTC TTGGGTCAGT GAGAGAAAAC TCCATGCTCA CAAAGTTTAC CACGTCGTGT
  961 CATTCCTTAA GTGGGATCTT CCTTTTTATG ATGAACCATA CAGTGGGTCT TTCGCCTTCC
 1021 CTCCTAAGTC TACACTGCTA TCATGGATTA TGAATCATTC ATGCTCGCCA GATGCATTAA
1081 GCAAGGCAGT ATGATGTTAA AACCCCCCGT TCAGA
//
Primer: pJB8-Forward ()
Sequence file: RDB15078_A7Eoc.seq check
>01971_15078_A7Eo_pJB8-Forward_E10_13_ABI24.ab1
    1 TTGACGGGGA ACTTTCCCGT CCGCCAGGGG GACATGCCGG CGATGCTGAA GGTCGCGCGC
   61 ATTCCCGATG AAGAGGCCGG TTACCGCCTG TTGACCTGGT GGGACGGGCA GGGCGCCGCC
  121 CGAGTCTTCG CCTCGGCGGC GGGCGCTCTG CTCATGGAGC GCGCGTCCGG GGCCGGGGAC
  181 CTTGCACAGA TAGCGTGGTC CGGCCAGGAC GACGAGGCTT GCAGGATCAT AATCAGCCAT
  241 ACCACATTTG TAGAGGTTTT ACTTGCTTTA AAAAACCTCC CACACCTCCC CCTGAACCTG
  301 AAACATAAAA TGAATGCAAT TGTTGTTGTT AACTTGTTTA TTGCAGCTTA TAATGGTTAC
  361 AAATAAAGCA ATAGCATCAC AAATTTCACA AATAAAGCAT TTTTTTCACT GCATTCTAGT
  421 TGTGGTTTGT CCAAACTCAT CAATGTATCT TATCATGTCT GGATCGATCC AGAGCTGTAA
  481 CAGCAGGGAC CCGCCCCAGT ATAGGTGCGG GCATTCCTTT AGACACCCTT GAAACTCTTG
  541 GGGCCTTGCG TCCAGGGGTG TATGAGGACA CTGTGCTACC AGAGGCCCCT GCAATAGTCA
  601 CTCCTGATGC TGTTCCTGCA GATTCAGGGC TTGATGCCCT GTCCATAGGT ACAGACTCGT
  661 CCACGGAGAC CCTCATTACT CTGCTAGAGC CTGAGGGTCC CGAGGACATA GCGGTTCTTG
  721 AGCTGCAACC CCTGGACCGT CCAACTTGGC AAGTAAGCAA TGCTGTTCAT CAGTCCTCTG
  781 CATACCACGC CCCTCTGCAG CTGCAATCGT CCATTGCAGA AACATCTGGT TTAGAAAAAT
  841 ATTTTTGTAG GAGGCTCGGG TTTAGGGGAT ACAGGAGGAG AAAACATTGA ACTGACATAC
  901 TTCGGGTCCC CACGAACAAG CACGCCCCGC AGTATTGCCT CTAAATCACG TGGCATTTTA
  961 AACTGGTTCA GTAAACGGTA CTACACACAG GTGCCCACGG AAGATCCTGA AGTGTTTTCA
 1021 TCCCAAACAT TGCAAACCCA CTGTATGAAG CAGACCCAGC TGTGCTTAGG ACCTAGTGAC
 1081 GTGTTGACTC AGTCAGTTTT ATAACCCTGA ATACACTACA CACGTAACCG GACAGAGTGG
1141 ACCCACAAGC CTACCATGTC CAAGTAACTC CTCTCAATG
//
Primer: BPV-1_F ()
Sequence file: RDB15078_A7Eod.seq check
>01971_15078_A7Eo_BPV-1_F_B05_05_ABI24.ab1
    1 CATGGAATGG TCTCTGGAGG GTTGCTGCAG GTGGATCACC AAGGTACACA CCACTCCGAA
   61 CAGCAGGGTC CACATCATCG CTTGCATCAA TAGGATCCAG ACATGATAAG ATACATTGAT
  121 GAGTTTGGAC AAACCACAAC TAGAATGCAG TGAAAAAAAT GCTTTATTTG TGAAATTTGT
  181 GATGCTATTG CTTTATTTGT AACCATTATA AGCTGCAATA AACAAGTTAA CAACAACAAT
  241 TGCATTCATT TTATGTTTCA GGTTCAGGGG GAGGTGTGGG AGGTTTTTTA AAGCAAGTAA
  301 AACCTCTACA AATGTGGTAT GGCTGATTAT GATCTCTAGT CAAGGCACTA TACATCAAAT
  361 ATTCCTTATT AACCCCTTTA CAAATTAAAA AGCTAAAGGT ACACAATTTT TGAGCATAGT
  421 TATTAATAGC AGACACTCTA TGCCTGTGTG GAGTAAGAAA AAACAGTATG TTATGATTAT
  481 AACTGTTATG CCTACTTATA AAGGTTACAG AATATTTTTC CATAATTTTC TTGTATAGCA
  541 GTGCAGCTTT TTCCTTTGTG GTGTAAATAG CAAAGCAAGC AAGAGTTCTA TTACTAAACA
  601 CAGCATGACT CAAAAAACTT AGCAATTCTG AAGGAAAGTC CTTGGGGTCT TCTACCTTTC
  661 TCTTCTTTTT TGGAGGAGTA GAATGTTGAG AGTCAGCAGT AGCCTCATCA TCACTAGATG
  721 GCATTTCTTC TGAGCAAAAC AGGTTTTCCT CATTAAAGGC ATTCCACCAC TGCTCCCATT
  781 CATCAGTTCC ATAGGTTGGA ATCTAAAATA CACAAACAAT TAGAATCAGT AGTTTAACAC
  841 ATTATACACT TAAAAATTTT ATATTTACCT TAGAGCTTTA AATCTCTGTA GTAGTTTGTC
  901 CAATTTATGT CACACCACAG AAAGTAAGGT TCCTTCACAA AGATCCTTCT AGAAGGATCC
961 CCGGG
//

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

original Nakamura, Y., Growth complementation of influenza virus temperature-sensitive mutants in mouse cells which express the RNA polymerase and nucleoprotein genes. J. Biochem., 110, 395-401 (1991). PMID 1663110. [link to RRC of NBRP]
user_report Kuzuhara, T., Preparation of Recombinant PA Endonuclease Domain Protein of Influenza A Virus and Its Application for Glycobiology Research. Methods Mol. Biol. 2556: 69-78 (2022). PMID 36175628. [link to RRC of NBRP]
user_report Shirayama, R., Inhibition of PA endonuclease activity of influenza virus RNA polymerase by Kampo medicines. Drug Discov. Ther. 10 (2): 109-113 (2016). PMID 26902927. [link to RRC of NBRP]
user_report Shoji, M., Anti-influenza activity of c60 fullerene derivatives. PLoS One, 8 (6): e66337 (2013). PMID 23785493. [link to RRC of NBRP]
user_report Iwai, Y., Anti-influenza activity of marchantins, macrocyclic bisbibenzyls contained in liverworts. PLoS One 6 (5): e19825 (2011). PMID 21625478. [link to RRC of NBRP]
user_report Iwai Y., Anti-influenza activity of phenethylphenylphthalimide analogs derived from thalidomide. Bioorg. Med. Chem., 18, 5379-5390 (2010). PMID 20538468. [link to RRC of NBRP]
user_report Kuzuhara T., Green tea catechins inhibit the endonuclease activity of influenza A virus RNA polymerase. PLoS Curr., 1, RRN1052 (2009). PMID 20025206. [link to RRC of NBRP]