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pBMSA-PB1

Influenza virus RNA polymerase PB1 subunit gene

Catalog number RDB01970
Resource name pBMSA-PB1
Clone info. Influenza virus (A/PR/8/34) RNA polymerase PB1 gene inducible plasmid. Nucleotide sequence 5 to 2341nt of AF389116.1 (CDS, 25 to 2298nt) is cloned.
Comment Related cell lines RCB1506, RCB1508, RCB1509 are available from RIKEN Cell Bank. Known differences (at least): A to C substitution at 549nt (Lys to Asn at 175aa).
Vector backbone pBMSA (Plasmid)
Size of vector backbone 13.2 kb
Selectable markers Amp^r
Gene/insert name Influenza virus RNA polymerase PB1 cDNA
Depositor|Developer Nakada, Susumu |
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External Database
Influenza virus RNA polymerase PB1

          Reference sequence
            

          Distribution information

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          RDB01970 pBMSA-PB1 DNA solution

          Please review the QC test results indicated by check icon below as well as clone information before placing your order.

          References and tips

          Electronic file

          Remarks, protocol and/or map (gif) RDB01970.gif

          Original reference

          original Nakamura, Y., Growth complementation of influenza virus temperature-sensitive mutants in mouse cells which express the RNA polymerase and nucleoprotein genes. J. Biochem., 110, 395-401 (1991). PMID 1663110.

          Further references such as user reports and related articles (go to bottom)

          Featured content


          Sequence information

          RIKEN BRC has sequenced portions of this material for quality test.
          Please review the QC test results indicated by check icon as well as clone information before placing your order.

          Test sheet RDB15077_A7Eop1-2.pdf check

          Nucleotide sequence of a portion of this resource (if available).

          Primer: MMTV-LTR#2
          Sequence file: RDB15077_A7Eoa.seq check
          >01970_15077_A7Eo_MMTV-LTR#2_C02_03_ABI08.ab1
              1 CTTGGGTGTT TGGTGTTCTG TTTCGCCATC CCGTCTCCGC TCGTCACTTA TCCTTCACTT
             61 TCCAGAGGGG TCCCCCCGCA GACCCCGGCG ACCCTCAGGT CGGCCGACTG CGGCAGCTCT
            121 AGAGAGCTTG GCAAAAGCAG GCAAACCATT TGAATGGATG TCAATCCGAC CTTACTTTTC
            181 TTAAAAGTGC CAGCACAAAA TGCTATAAGC ACAACTTTCC CTTATACTGG AGACCCTCCT
            241 TACAGCCATG GGACAGGAAC AGGATACACC ATGGATACTG TCAACAGGAC ACATCAGTAC
            301 TCAGAAAAGG GAAGATGGAC AACAAACACC GAAACTGGAG CACCGCAACT CAACCCGATT
            361 GATGGGCCAC TGCCAGAAGA CAATGAACCA AGTGGTTATG CCCAAACAGA TTGTGTATTG
            421 GAAGCAATGG CTTTCCTTGA GGAATCCCAT CCTGGTATTT TTGAAAACTC GTGTATTGAA
            481 ACGATGGAGG TTGTTCAGCA AACACGAGTA GACAAGCTGA CACAAGGCCG ACAGACCTAT
            541 GACTGGACTC TAAATAGAAA CCAACCTGCT GCAACAGCAT TGGCCAACAC AATAGAAGTG
            601 TTCAGATCAA ATGGCCTCAC GGCCAATGAG TCTGGAAGGC TCATAGACTT CCTTAAGGAT
            661 GTAATGGAGT CAATGAACAA AGAAGAAATG GGGATCACAA CTCATTTTCA GAGAAAGAGA
            721 CGGGTGAGAG ACAATATGAC TAAGAAAATG ATAACACAGA GAACAATAGG GTAAAAAGAA
            781 GCAGAGATTG AACAAAAGGA GTTATCTAAT TAGAGCATTG ACCCTGAACA CAATGACCAA
            841 AGATGCTGAG AGAGGGAAGC TAAAACGGAG AGCAATTGCA ACCCCCAGGG ATGCAAATAA
            901 GGGGGGTTTG TATACTTTGT TGAGACACTG GGCCAAGAAG TATATGTGAG AAAACTTGAA
            961 CATCAGGTTT GCCCAGTTTG GAGGCAATGA GGAGAAAAGC AAAGGTTGGC CAAATGTTGG
           1021 TAAGGAAGAA TGATGACCAA TCTCAGGACA CCGAACCTTT TCTTTCACAT CACTGGGGAG
          1081 AATAACACCA TGACGAAAAT CGATCTCGAT GGTTTTGGCC ATTGT
          //
          Primer: SV40NLS_R
          Sequence file: RDB15077_A7Eob.seq check
          >01970_15077_A7Eo_SV40NLS#1_G04_19_ABI24.ab1
              1 GGGGGGAATA GCAGTAGCCT CATCATCACT AGATGGCATT TCTTCTGAGC AAAACAGGTT
             61 TTCCTCATTA AAGGCATTCC ACCACTGCTC CCATTCATCA GTTCCATAGG TTGGAATCTA
            121 AAATACACAA ACAATTAGAA TCAGTAGTTT AACACATTAT ACACTTAAAA ATTTTATATT
            181 TACCTTAGAG CTTTAAATCT CTGTAGGTAG TTTGTCCAAT TATGTCACAC CACAGAAGTA
            241 AGGTTCCTTC ACAAAGATCC TCTAGAGTCG ACCTGCAGCC CAAGCTTGAA TTCCAGTAGA
            301 AACAAGGCAT TTTTTCATGA AGGACAAGCT AAATTCACTA TTTTTGCCGT CTGAGCTCTT
            361 CAATGGTGGA ACAGATCTTC ATGATCTCAG TGAACTCTTC TTTCTTTATC CTTCCAGATT
            421 CGAAATCAAT CCGTGCATCA ATTCGGGCTC TGGAAACCAT AGCCTCCACC ATACTGGATA
            481 TCCCGACTGG TCTTCTGTAT GAACTGCTGG GGAAGAATTT TTCAAATAAA TTGCAGCACC
            541 TTTGGTACAT TTGTTCATCT TCAAGTACTC CTCTTTGACT TGTATTCAAG ATGGATCGAT
            601 TTCTTTTGGG GATCCAGGAG TGTGTTGTTG CAACAGCATC ATACTCCATG TTTTTGGCTG
            661 GACCATGTGC TGGCATCATC ACTGCATTGT TCATTGATTC AATTTCTTTA TGGCTGACAA
            721 ATGGGTTCAG TGGGTTGCAT AAACGCCCCT GGTAATCCTC ATCCATCAAT TCCCATTTTA
            781 GGCAGACTTC AGGAATGTGG AGATTTCTAA TGTTGTATAA ATTTGGGCCT CCGTCGGAGA
            841 CCAGCAGTCC AGCTTTGGAA CGGGTTTGCT CCCACAGTTT CTTTATTTCA AATGATCTTC
            901 GGGTTTGTAT TTGTGTGTCA CCTCTATGGC ATCGGTACGT GTACCTGTAA TCTTTGGATG
            961 AACAACTGAA AGGGCCATTT GAGCTGTTGC TGACCAAGAT CATTGTTAAT CATATTGTTT
           1021 TGGATGACAG TAACTTCAAT ACTCATGTCC GCTGACTCGT TGGATCCAAG AACACCCGAA
           1081 CTGGGGGGAG CTTCAATGCT GAATGGGCAC CATCCGTTAA CGAATGGAAG AGCTGGTGGA
          1141 ATTCA
          //
          Primer: pJB8-Forward
          Sequence file: RDB15077_A7Eoc.seq check
          >01970_15077_A7Eo_pJB8-Forward_H09_24_ABI24.ab1
              1 TACGTGACTT CCGTCCGCCA GGGGGACATG CCGGCGATGC TGAAGGTCGC GCGCATTCCC
             61 GATGAAGAGG CCGGTTACCG CCTGTTGACC TGGTGGGACG GGCAGGGCGC CGCCCGAGTC
            121 TTCGCCTCGG CGGCGGGCGC TCTGCTCATG GAGCGCGCGT CCGGGGCCGG GGACCTTGCA
            181 CAGATAGCGT GGTCCGGCCA GGACGACGAG GCTTGCAGGA TCATAATCAG CCATACCACA
            241 TTTGTAGAGG TTTTACTTGC TTTAAAAAAC CTCCCACACC TCCCCCTGAA CCTGAAACAT
            301 AAAATGAATG CAATTGTTGT TGTTAACTTG TTTATTGCAG CTTATAATGG TTACAAATAA
            361 AGCAATAGCA TCACAAATTT CACAAATAAA GCATTTTTTT CACTGCATTC TAGTTGTGGT
            421 TTGTCCAAAC TCATCAATGT ATCTTATCAT GTCTGGATCG ATCCAGAGCT GTAACAGCAG
            481 GGACCCGCCC CAGTATAGGT GCGGGCATTC CTTTAGACAC CCTTGAAACT CTTGGGGCCT
            541 TGCGTCCAGG GGTGTATGAG GACACTGTGC TACCAGAGGC CCCTGCAATA GTCACTCCTG
            601 ATGCTGTTCC TGCAGATTCA GGGCTTGATG CCCTGTCCAT AGGTACAGAC TCGTCCACGG
            661 AGACCCTCAT TACTCTGCTA GAGCCTGAGG GTCCCGAGGA CATAGCGGTT CTTGAGCTGC
            721 AACCCCTGGA CCGTCCAACT TGGCAAGTAA GCAATGCTGT TCATCAGTCC TCTGCATACC
            781 ACGCCCCTCT GCAGCTGCAA TCGTCCATTG CAGAAACATC TGGTTTAGAA AATATTTTTG
          841 TAGGAGGTCG GGGTGTTTAA GGGGGATACC GG
          //
          Primer: BPV-1_F
          Sequence file: RDB15077_A7Eod.seq check
          >01970_15077_A7Eo_BPV-1_F_A02_01_ABI08.ab1
              1 TTGCCCATGG ATCTCTGGAG GGTTGCTGCA GGTGGATCAC CAAGGTACAC ACCACTCCGA
             61 ACAGCAGGGT CCACATCATC GCTTGCATCA ATAGGATCCA GACATGATAA GATACATTGA
            121 TGAGTTTGGA CAAACCACAA CTAGAATGCA GTGAAAAAAA TGCTTTATTT GTGAAATTTG
            181 TGATGCTATT GCTTTATTTG TAACCATTAT AAGCTGCAAT AAACAAGTTA ACAACAACAA
            241 TTGCATTCAT TTTATGTTTC AGGTTCAGGG GGAGGTGTGG GAGGTTTTTT AAAGCAAGTA
            301 AAACCTCTAC AAATGTGGTA TGGCTGATTA TGATCTCTAG TCAAGGCACT ATACATCAAA
            361 TATTCCTTAT TAACCCCTTT ACAAATTAAA AAGCTAAAGG TACACAATTT TTGAGCATAG
            421 TTATTAATAG CAGACACTCT ATGCCTGTGT GGAGTAAGAA AAAACAGTAT GTTATGATTA
            481 TAACTGTTAT GCCTACTTAT AAAGGTTACA GAATATTTTT CCATAATTTT CTTGTATAGC
            541 AGTGCAGCTT TTTCCTTTGT GGTGTAAATA GCAAAGCAAG CAAGAGTTCT ATTACTAAAC
            601 ACAGCATGAC TCAAAAAACT TAGCAATTCT GAAGGAAAGT CCTTGGGGTC TTCTACCTTT
            661 CTCTTCTTTT TTGGAGGAGT AGAATGTTGA GAGTCAGCAG TAGCCTCATC ATCACTAGAT
            721 GGCATTTCTT CTGAGCAAAA CAGGTTTTCC TCATTAAAGG CATTCCACCA CTGCTCCCAT
            781 TTCATCAGTT CCATAGGTTG GGAAATCTAA AATACACAAA CAATTAGAAT CAGTAGTTTA
            841 ACACATTATA CACTTTAAAA AATTTTATAT TTTACCTTTA GAGGCTTTAA AATCTCTGTA
            901 GGTAGGTTTG GTCCAATTTA TGTTCACCAC CACAGAAAGT AAAGGTTCCT TTCCACAAAG
            961 GATCCCTCCT AAAAGGTCGA CCTGCCAGCC CAAGCTTTGG AATTCCAATA GAAAAACAGG
           1021 GCATTTTTTT CAGGAAGGGA CAAAGCTTAA ATTCAACTAG TTTTTTTGGC CGTTCTTGAG
           1081 ACTCTCTTCA AATTGCGTGG GGAAAACAGA AATTCCTGTT CATTGATCTT CAAGATTGAA
          1141 ACTCTCTGTC CCTTTATTCG TGTTGAAGAT TCCGCTTCTC ACAACATGAT TC
          //

          Please visit Sequencing and PCR primers for primer information.


          References

          Original, user report and related articles

          original Nakamura, Y., Growth complementation of influenza virus temperature-sensitive mutants in mouse cells which express the RNA polymerase and nucleoprotein genes. J. Biochem., 110, 395-401 (1991). PMID 1663110.

          2022.05.18

          GNP_filter3_RDBDEP_html_220516.pl