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pBMSA-PB1 (#RDB01970)

Influenza virus RNA polymerase PB1 subunit gene

Clone info. Influenza virus (A/PR/8/34) RNA polymerase PB1 gene inducible plasmid. Nucleotide sequence 5 to 2341nt of AF389116.1 (CDS, 25 to 2298nt) is cloned.
Comment Related cell lines RCB1506, RCB1508, RCB1509 are available from RIKEN Cell Bank. Known differences (at least): A to C substitution at 549nt (Lys to Asn at 175aa).
Vector backbone pBMSA (Plasmid)
Size of vector backbone 13.2 kb
Selectable markers Amp^r
Gene/insert name Influenza virus RNA polymerase PB1 cDNA
Depositor|Developer Nakada, Susumu |
 
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Catalog # Resource name Shipping form Fee (non-profit org.)
RDB01970 pBMSA-PB1 DNA solution JPY 9,460 (not-for-profit academic purpose)
plus cost of shipping containers, dry ice (if required) and shipping charge

Please review the QC test results indicated by check icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The pBMSA-PB1 was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB01970).

Reference section:

Nakamura, Y., Oda, K., Nakada, S., Growth complementation of influenza virus temperature-sensitive mutants in mouse cells which express the RNA polymerase and nucleoprotein genes. J. Biochem., 110, 395-401 (1991). PMID 1663110. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB15077_A7Eop1-2.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: MMTV-LTR#2 ()
Sequence file: RDB15077_A7Eoa.seq check
>01970_15077_A7Eo_MMTV-LTR#2_C02_03_ABI08.ab1
    1 CTTGGGTGTT TGGTGTTCTG TTTCGCCATC CCGTCTCCGC TCGTCACTTA TCCTTCACTT
   61 TCCAGAGGGG TCCCCCCGCA GACCCCGGCG ACCCTCAGGT CGGCCGACTG CGGCAGCTCT
  121 AGAGAGCTTG GCAAAAGCAG GCAAACCATT TGAATGGATG TCAATCCGAC CTTACTTTTC
  181 TTAAAAGTGC CAGCACAAAA TGCTATAAGC ACAACTTTCC CTTATACTGG AGACCCTCCT
  241 TACAGCCATG GGACAGGAAC AGGATACACC ATGGATACTG TCAACAGGAC ACATCAGTAC
  301 TCAGAAAAGG GAAGATGGAC AACAAACACC GAAACTGGAG CACCGCAACT CAACCCGATT
  361 GATGGGCCAC TGCCAGAAGA CAATGAACCA AGTGGTTATG CCCAAACAGA TTGTGTATTG
  421 GAAGCAATGG CTTTCCTTGA GGAATCCCAT CCTGGTATTT TTGAAAACTC GTGTATTGAA
  481 ACGATGGAGG TTGTTCAGCA AACACGAGTA GACAAGCTGA CACAAGGCCG ACAGACCTAT
  541 GACTGGACTC TAAATAGAAA CCAACCTGCT GCAACAGCAT TGGCCAACAC AATAGAAGTG
  601 TTCAGATCAA ATGGCCTCAC GGCCAATGAG TCTGGAAGGC TCATAGACTT CCTTAAGGAT
  661 GTAATGGAGT CAATGAACAA AGAAGAAATG GGGATCACAA CTCATTTTCA GAGAAAGAGA
  721 CGGGTGAGAG ACAATATGAC TAAGAAAATG ATAACACAGA GAACAATAGG GTAAAAAGAA
  781 GCAGAGATTG AACAAAAGGA GTTATCTAAT TAGAGCATTG ACCCTGAACA CAATGACCAA
  841 AGATGCTGAG AGAGGGAAGC TAAAACGGAG AGCAATTGCA ACCCCCAGGG ATGCAAATAA
  901 GGGGGGTTTG TATACTTTGT TGAGACACTG GGCCAAGAAG TATATGTGAG AAAACTTGAA
  961 CATCAGGTTT GCCCAGTTTG GAGGCAATGA GGAGAAAAGC AAAGGTTGGC CAAATGTTGG
 1021 TAAGGAAGAA TGATGACCAA TCTCAGGACA CCGAACCTTT TCTTTCACAT CACTGGGGAG
1081 AATAACACCA TGACGAAAAT CGATCTCGAT GGTTTTGGCC ATTGT
//
Primer: SV40NLS_R ()
Sequence file: RDB15077_A7Eob.seq check
>01970_15077_A7Eo_SV40NLS#1_G04_19_ABI24.ab1
    1 GGGGGGAATA GCAGTAGCCT CATCATCACT AGATGGCATT TCTTCTGAGC AAAACAGGTT
   61 TTCCTCATTA AAGGCATTCC ACCACTGCTC CCATTCATCA GTTCCATAGG TTGGAATCTA
  121 AAATACACAA ACAATTAGAA TCAGTAGTTT AACACATTAT ACACTTAAAA ATTTTATATT
  181 TACCTTAGAG CTTTAAATCT CTGTAGGTAG TTTGTCCAAT TATGTCACAC CACAGAAGTA
  241 AGGTTCCTTC ACAAAGATCC TCTAGAGTCG ACCTGCAGCC CAAGCTTGAA TTCCAGTAGA
  301 AACAAGGCAT TTTTTCATGA AGGACAAGCT AAATTCACTA TTTTTGCCGT CTGAGCTCTT
  361 CAATGGTGGA ACAGATCTTC ATGATCTCAG TGAACTCTTC TTTCTTTATC CTTCCAGATT
  421 CGAAATCAAT CCGTGCATCA ATTCGGGCTC TGGAAACCAT AGCCTCCACC ATACTGGATA
  481 TCCCGACTGG TCTTCTGTAT GAACTGCTGG GGAAGAATTT TTCAAATAAA TTGCAGCACC
  541 TTTGGTACAT TTGTTCATCT TCAAGTACTC CTCTTTGACT TGTATTCAAG ATGGATCGAT
  601 TTCTTTTGGG GATCCAGGAG TGTGTTGTTG CAACAGCATC ATACTCCATG TTTTTGGCTG
  661 GACCATGTGC TGGCATCATC ACTGCATTGT TCATTGATTC AATTTCTTTA TGGCTGACAA
  721 ATGGGTTCAG TGGGTTGCAT AAACGCCCCT GGTAATCCTC ATCCATCAAT TCCCATTTTA
  781 GGCAGACTTC AGGAATGTGG AGATTTCTAA TGTTGTATAA ATTTGGGCCT CCGTCGGAGA
  841 CCAGCAGTCC AGCTTTGGAA CGGGTTTGCT CCCACAGTTT CTTTATTTCA AATGATCTTC
  901 GGGTTTGTAT TTGTGTGTCA CCTCTATGGC ATCGGTACGT GTACCTGTAA TCTTTGGATG
  961 AACAACTGAA AGGGCCATTT GAGCTGTTGC TGACCAAGAT CATTGTTAAT CATATTGTTT
 1021 TGGATGACAG TAACTTCAAT ACTCATGTCC GCTGACTCGT TGGATCCAAG AACACCCGAA
 1081 CTGGGGGGAG CTTCAATGCT GAATGGGCAC CATCCGTTAA CGAATGGAAG AGCTGGTGGA
1141 ATTCA
//
Primer: pJB8-Forward ()
Sequence file: RDB15077_A7Eoc.seq check
>01970_15077_A7Eo_pJB8-Forward_H09_24_ABI24.ab1
    1 TACGTGACTT CCGTCCGCCA GGGGGACATG CCGGCGATGC TGAAGGTCGC GCGCATTCCC
   61 GATGAAGAGG CCGGTTACCG CCTGTTGACC TGGTGGGACG GGCAGGGCGC CGCCCGAGTC
  121 TTCGCCTCGG CGGCGGGCGC TCTGCTCATG GAGCGCGCGT CCGGGGCCGG GGACCTTGCA
  181 CAGATAGCGT GGTCCGGCCA GGACGACGAG GCTTGCAGGA TCATAATCAG CCATACCACA
  241 TTTGTAGAGG TTTTACTTGC TTTAAAAAAC CTCCCACACC TCCCCCTGAA CCTGAAACAT
  301 AAAATGAATG CAATTGTTGT TGTTAACTTG TTTATTGCAG CTTATAATGG TTACAAATAA
  361 AGCAATAGCA TCACAAATTT CACAAATAAA GCATTTTTTT CACTGCATTC TAGTTGTGGT
  421 TTGTCCAAAC TCATCAATGT ATCTTATCAT GTCTGGATCG ATCCAGAGCT GTAACAGCAG
  481 GGACCCGCCC CAGTATAGGT GCGGGCATTC CTTTAGACAC CCTTGAAACT CTTGGGGCCT
  541 TGCGTCCAGG GGTGTATGAG GACACTGTGC TACCAGAGGC CCCTGCAATA GTCACTCCTG
  601 ATGCTGTTCC TGCAGATTCA GGGCTTGATG CCCTGTCCAT AGGTACAGAC TCGTCCACGG
  661 AGACCCTCAT TACTCTGCTA GAGCCTGAGG GTCCCGAGGA CATAGCGGTT CTTGAGCTGC
  721 AACCCCTGGA CCGTCCAACT TGGCAAGTAA GCAATGCTGT TCATCAGTCC TCTGCATACC
  781 ACGCCCCTCT GCAGCTGCAA TCGTCCATTG CAGAAACATC TGGTTTAGAA AATATTTTTG
841 TAGGAGGTCG GGGTGTTTAA GGGGGATACC GG
//
Primer: BPV-1_F ()
Sequence file: RDB15077_A7Eod.seq check
>01970_15077_A7Eo_BPV-1_F_A02_01_ABI08.ab1
    1 TTGCCCATGG ATCTCTGGAG GGTTGCTGCA GGTGGATCAC CAAGGTACAC ACCACTCCGA
   61 ACAGCAGGGT CCACATCATC GCTTGCATCA ATAGGATCCA GACATGATAA GATACATTGA
  121 TGAGTTTGGA CAAACCACAA CTAGAATGCA GTGAAAAAAA TGCTTTATTT GTGAAATTTG
  181 TGATGCTATT GCTTTATTTG TAACCATTAT AAGCTGCAAT AAACAAGTTA ACAACAACAA
  241 TTGCATTCAT TTTATGTTTC AGGTTCAGGG GGAGGTGTGG GAGGTTTTTT AAAGCAAGTA
  301 AAACCTCTAC AAATGTGGTA TGGCTGATTA TGATCTCTAG TCAAGGCACT ATACATCAAA
  361 TATTCCTTAT TAACCCCTTT ACAAATTAAA AAGCTAAAGG TACACAATTT TTGAGCATAG
  421 TTATTAATAG CAGACACTCT ATGCCTGTGT GGAGTAAGAA AAAACAGTAT GTTATGATTA
  481 TAACTGTTAT GCCTACTTAT AAAGGTTACA GAATATTTTT CCATAATTTT CTTGTATAGC
  541 AGTGCAGCTT TTTCCTTTGT GGTGTAAATA GCAAAGCAAG CAAGAGTTCT ATTACTAAAC
  601 ACAGCATGAC TCAAAAAACT TAGCAATTCT GAAGGAAAGT CCTTGGGGTC TTCTACCTTT
  661 CTCTTCTTTT TTGGAGGAGT AGAATGTTGA GAGTCAGCAG TAGCCTCATC ATCACTAGAT
  721 GGCATTTCTT CTGAGCAAAA CAGGTTTTCC TCATTAAAGG CATTCCACCA CTGCTCCCAT
  781 TTCATCAGTT CCATAGGTTG GGAAATCTAA AATACACAAA CAATTAGAAT CAGTAGTTTA
  841 ACACATTATA CACTTTAAAA AATTTTATAT TTTACCTTTA GAGGCTTTAA AATCTCTGTA
  901 GGTAGGTTTG GTCCAATTTA TGTTCACCAC CACAGAAAGT AAAGGTTCCT TTCCACAAAG
  961 GATCCCTCCT AAAAGGTCGA CCTGCCAGCC CAAGCTTTGG AATTCCAATA GAAAAACAGG
 1021 GCATTTTTTT CAGGAAGGGA CAAAGCTTAA ATTCAACTAG TTTTTTTGGC CGTTCTTGAG
 1081 ACTCTCTTCA AATTGCGTGG GGAAAACAGA AATTCCTGTT CATTGATCTT CAAGATTGAA
1141 ACTCTCTGTC CCTTTATTCG TGTTGAAGAT TCCGCTTCTC ACAACATGAT TC
//

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

original Nakamura, Y., Growth complementation of influenza virus temperature-sensitive mutants in mouse cells which express the RNA polymerase and nucleoprotein genes. J. Biochem., 110, 395-401 (1991). PMID 1663110. [link to RRC of NBRP]