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pBMSA-PB2 (#RDB01969)

Influenza virus RNA polymerase PB2 subunit gene

Clone info. Influenza virus (A/PR/8/34) RNA polymerase PB2 gene inducible plasmid. Nucleotide sequence 5 to 2341nt of AF389115.1 (CDS, 28 to 2307nt) is cloned.
Comment Related cell line RCB1508 is available from RIKEN Cell Bank.
Vector backbone pBMSA (Plasmid)
Size of vector backbone 13.2 kb
Selectable markers Amp^r
Gene/insert name Influenza virus RNA polymerase PB2 cDNA
Depositor|Developer Nakada, Susumu |
 
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Catalog # Resource name Shipping form Fee (non-profit org.)
RDB01969 pBMSA-PB2 DNA solution JPY 9,460 (not-for-profit academic purpose)
plus cost of shipping containers, dry ice (if required) and shipping charge

Please review the QC test results indicated by check icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The pBMSA-PB2 was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB01969).

Reference section:

Nakamura, Y., Oda, K., Nakada, S., Growth complementation of influenza virus temperature-sensitive mutants in mouse cells which express the RNA polymerase and nucleoprotein genes. J. Biochem., 110, 395-401 (1991). PMID 1663110. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB15076_A7Eop1-2.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: MMTV-LTR#2 ()
Sequence file: RDB15076_A7Eoa.seq check
>01969_15076_A7Eo_1_MMLV-LTR#2_C04_07_ABI24.ab1
    1 CGTGGGGGTT TGTGTCTGTT CGCCATCCCG TCTCCGCTCG TCACTTATCC TTCACTTTCC
   61 AGAGGGTCCC CCCGCAGACC CCGGCGACCC TCAGGTCGGC CGACTGCGGC AGCTCTAGAG
  121 GATCCCGAAT TCCAGCAAAA GCAGGTCAAT TATATTCAAT ATGGAAAGAA TAAAAGAACT
  181 AAGAAATCTA ATGTCGCAGT CTCGCACCCG CGAGATACTC ACAAAAACCA CCGTGGACCA
  241 TATGGCCATA ATCAAGAAGT ACACATCAGG AAGACAGGAG AAGAACCCAG CACTTAGGAT
  301 GAAATGGATG ATGGCAATGA AATATCCAAT TACAGCAGAC AAGAGGATAA CGGAAATGAT
  361 TCCTGAGAGA AATGAGCAAG GACAAACTTT ATGGAGTAAA ATGAATGATG CAGGATCAGA
  421 CCGAGTGATG GTATCACCTC TGGCTGTGAC ATGGTGGAAT AGGAATGGAC CAATAACAAA
  481 TACAGTTCAT TATCCAAAAA TCTACAAAAC TTATTTTGAA AGAGTCGAAA GGCTAAAGCA
  541 TGGAACCTTT GGCCCTGTCC ATTTTAGAAA CCAAGTCAAA ATACGTCGGA GAGTTGACAT
  601 AAATCCTGGT CATGCAGATC TCAGTGCCAA GGAGGCACAG GATGTAATCA TGGAAGTTGT
  661 TTTCCCTAAC GAAGTGGGAG CCAGGATACT AACATCGGAA TCGCAACTAA CGATAACCAA
  721 AGAGAAGAAA GAAGAACTCC AGGATTGCAA AATTTCTCCT TTGATGGTTG CATACATGTT
  781 GGAGAGAGAA CTGGTCCGCA AAACGAGATT CCTCCCAGTG GCTGGTGGAA CAAGCAGTGT
  841 GTACATTGAA GTGTTGCATT TGACTCAAGG AACATGCTGG GAACAGATGT ATACTCCAGG
  901 AGGGGAAGTG AGGAATGATG ATGTTGATCA AAGCTTGATT ATTGCTGCTA GGAACATAGT
  961 GAGAAGAGCT GCAGTATCAG CAGATCCACT AGCATCTTTT ATTTGGAGAT GTGCCACAGC
 1021 ACACAGATTG GGTGGGAAAT TAGGATGGTA GACATCCTTA GCAGAACCAC CAGAAGAGCA
 1081 AGCGTGGAAT ATATGCAGCC TGCCATGGGA CTGAGATTAG CTTCATCTCA GTTGTGATCA
1141 CAAGTGGA
//
Primer: SV40NLS_R ()
Sequence file: RDB15076_A7Eob.seq check
>01969_15076_A7Eo_sv40NLS_R_B01_02_ABI08.ab1
    1 ATAAGGGAGT AACCAGGTAG CCTCATCATC ACTAGATGGC ATTTCTTCTG AGCAAAACAG
   61 GTTTTCCTCA TTAAAGGCAT TCCACCACTG CTCCCATTCA TCAGTTCCAT AGGTTGGAAT
  121 CTAAAATACA CAAACAATTA GAATCAGTAG TTTAACACAT TATACACTTA AAAATTTTAT
  181 ATTTACCTTA GAGCTTTAAA TCTCTGTAGG TAGTTTGTCC AATTATGTCA CACCACAGAA
  241 GTAAGGTTCC TTCACAAAGA TCCTCTAGAG GATCCCGAAT TCCAGTAGAA ACAAGGTCGT
  301 TTTTAAACTA TTCGACACTA ATTGATGGCC ATCCGAATTC TTTTGGTCGC TGTCTGGCTG
  361 TCAGTAAGTA TGCTAGAGTC CCGTTTCCGT TTCATTACCA ACACCACGTC TCCTTGCCCA
  421 ATTAGCACAT TAGCCTTCTC TCCTTTCGCA AGGTTGCTCA GTTCATTGAT GCTTAGTGCT
  481 GGCCCATATC TCTTGTCTTC TTTGCCCAGA ATGAGGAATC CCCTCAGAAC AGCGGACTCC
  541 ACTCCAGCTG TGCCTTCATC TGGGTCTTCA GTTAAAGTGC CAGCATCCTT TCCGAGAACT
  601 GTGAGTCTCT TCGTGGCCTT GTTATAGTTG AATACAGGAG AATTGCCCCT TACAAGTATT
  661 CTCATTCCTG ATCCCCTCAC ATTCACAGTA AATGAGGAGA ACTGCATTCT ACTTTGCTTT
  721 GGTGGAGCGG CTGCGAAGGG AAGAAGTTTT ATTATCTGTG CGGTATCAAA TGTCCCAAGC
  781 ACATCCCTCA TTTGTTGGGA ACAGAGTTCT TACAAACCCA CTGTATTGGG CCTCTAATGG
  841 CCTTAGGTAC TAAAAGACTG AAATGGTTTC AAATTCCATT TTATTGTATA GCATTGTAGG
  901 GTTCTGGGAC CACTGAAATT TTAACAGTTT CCCAGTTTCT GATGATCCAT TGATAGGTAT
  961 TGACCAACAC TGATTCAGGA CCATTAAATC TCCCCACAAT CAATTGACGA TGGAGTAAGG
 1021 TTATTGGTCA GTTTCCTCTG TTTCCTGGTG TTTTCAACTG GACCTCTTCG GAAGAACAGT
1081 TAGTTAACAA TTTCTCGATG GTCCGAATTC TTCAAAAAAC CGGTCAGAAT GCTTTCA
//
Primer: pJB8-Forward ()
Sequence file: RDB15076_A7Eoc.seq check
>01969_15076_A7Eo_1_pJB8-Forward_C09_09_ABI24.ab1
    1 TGCCTGGACT TCCGTCCGCC AGGGGGACAT GCCGGCGATG CTGAAGGTCG CGCGCATTCC
   61 CGATGAAGAG GCCGGTTACC GCCTGTTGAC CTGGTGGGAC GGGCAGGGCG CCGCCCGAGT
  121 CTTCGCCTCG GCGGCGGGCG CTCTGCTCAT GGAGCGCGCG TCCGGGGCCG GGGACCTTGC
  181 ACAGATAGCG TGGTCCGGCC AGGACGACGA GGCTTGCAGG ATCATAATCA GCCATACCAC
  241 ATTTGTAGAG GTTTTACTTG CTTTAAAAAA CCTCCCACAC CTCCCCCTGA ACCTGAAACA
  301 TAAAATGAAT GCAATTGTTG TTGTTAACTT GTTTATTGCA GCTTATAATG GTTACAAATA
  361 AAGCAATAGC ATCACAAATT TCACAAATAA AGCATTTTTT TCACTGCATT CTAGTTGTGG
  421 TTTGTCCAAA CTCATCAATG TATCTTATCA TGTCTGGATC GATCCAGAGC TGTAACAGCA
  481 GGGACCCGCC CCAGTATAGG TGCGGGCATT CCTTTAGACA CCCTTGAAAC TCTTGGGGCC
  541 TTGCGTCCAG GGGTGTATGA GGACACTGTG CTACCAGAGG CCCCTGCAAT AGTCACTCCT
  601 GATGCTGTTC CTGCAGATTC AGGGCTTGAT GCCCTGTCCA TAGGTACAGA CTCGTCCACG
  661 GAGACCCTCA TTACTCTGCT AGAGCCTGAG GGTCCCGAGG ACATAGCGGT TCTTGAGCTG
  721 CAACCCCTGG ACCGTCCAAC TTGGCAAGTA AGCAATGCTG TTCATCAGTC CTCTGCATAC
  781 CACGCCCCTC TGCAGCTGCA ATCGTCCATT GCAGAAACAT CTGGTTTAGA AAATATTTTT
  841 GTAGGAGGCT CGGGTTTAGG GGATACAGGA GGAGAAAACA TTGAACTGAC ATACTTCGGG
  901 TCCCCACGAA CAAGCACGCC CCGCAGTATT GCCTCTAAAT CACGTGGCAT TTTAAACTGG
  961 TTCAGTAACG GTACTACACA CAGGTGCCCA CGGGAAGATC CTGAGTGTTT TTCATCCCAA
 1021 ACATTTGCAA ACCCACTGTT ATGAGCAGAT CAGCTGTGCT AGGACTAGTG GACGTGTGAA
 1081 CTCAGTCAGT TTATAACCTG ATACCTTACA ACCGTAGCAG GACCGAGGTG GACCCACAAG
1141 CTACATGGTT CCAAGGTACT
//
Primer: BPV-1_F ()
Sequence file: RDB15076_A7Eod.seq check
>01969_15076_A7Eo_1_BPV-1_F_D04_10_ABI24.ab1
    1 CATGCCATGG TCTACTGGAG GGTTGCTGCA GGTGGATCAC CAAGGTACAC ACCACTCCGA
   61 ACAGCAGGGT CCACATCATC GCTTGCATCA ATAGGATCCA GACATGATAA GATACATTGA
  121 TGAGTTTGGA CAAACCACAA CTAGAATGCA GTGAAAAAAA TGCTTTATTT GTGAAATTTG
  181 TGATGCTATT GCTTTATTTG TAACCATTAT AAGCTGCAAT AAACAAGTTA ACAACAACAA
  241 TTGCATTCAT TTTATGTTTC AGGTTCAGGG GGAGGTGTGG GAGGTTTTTT AAAGCAAGTA
  301 AAACCTCTAC AAATGTGGTA TGGCTGATTA TGATCTCTAG TCAAGGCACT ATACATCAAA
  361 TATTCCTTAT TAACCCCTTT ACAAATTAAA AAGCTAAAGG TACACAATTT TTGAGCATAG
  421 TTATTAATAG CAGACACTCT ATGCCTGTGT GGAGTAAGAA AAAACAGTAT GTTATGATTA
  481 TAACTGTTAT GCCTACTTAT AAAGGTTACA GAATATTTTT CCATAATTTT CTTGTATAGC
  541 AGTGCAGCTT TTTCCTTTGT GGTGTAAATA GCAAAGCAAG CAAGAGTTCT ATTACTAAAC
  601 ACAGCATGAC TCAAAAAACT TAGCAAATTC TGAAGGAAAG TCCTTGGGGT CTTCTACCTT
  661 TCTCTTCTTT TTTGGAGGAG TAGAATGTTG AGAGTCAGCA GTAGCCTCAT CATCACTAGG
  721 ATGGCATTTT CTTCTGAGCA AAACAGGTTT TCCTCATTAA AAGGCATTCC ACCACTGCTC
781 CCAATTCATC AGTTTCCATA GGTTGGGAAT CTAAATACAC AAGCAAATTA GAATCAGTA
//

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

original Nakamura, Y., Growth complementation of influenza virus temperature-sensitive mutants in mouse cells which express the RNA polymerase and nucleoprotein genes. J. Biochem., 110, 395-401 (1991). PMID 1663110. [link to RRC of NBRP]
user_report Tsurumura, T., Crystallization and preliminary X-ray diffraction studies of a surface mutant of the middle domain of PB2 from human influenza A (H1N1) virus. Acta. Crystallogr. F Struct. Biol. Commun., 70 (Pt 1): 72-75 (2014). PMID 24419622. [link to RRC of NBRP]
user_report Tsurumura, T., Conformational Polymorphism of m(7)GTP in Crystal Structure of the PB2 Middle Domain from Human Influenza A Virus. PLoS One, 8 (11): e82020 (2013). PMID 24312396. [link to RRC of NBRP]
user_report Iwai Y., Anti-influenza activity of phenethylphenylphthalimide analogs derived from thalidomide. Bioorg. Med. Chem., 18, 5379-5390 (2010). PMID 20538468. [link to RRC of NBRP]
user_report Kuzuhara T., Structural basis of the influenza A virus RNA polymerase PB2 RNA-binding domain containing the pathogenicity-determinant lysine 627 residue. J. Biol. Chem. 284, 6855-6860 (2009) PMID 19144639. [link to RRC of NBRP]
user_report Kuzuhara T., Crystallization and X-ray diffraction analysis of the RNA primer/promoter-binding domain of influenza A virus RNA-dependent RNA polymerase PB2. Acta Crystallogr. Sect. F. Struct. Biol. Cryst. Commun., 65 (Pt 2): 144-146 (2009). PMID 19194006. [link to RRC of NBRP]