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pBMSA-PB2

Influenza virus RNA polymerase PB2 subunit gene

Catalog number RDB01969
Resource name pBMSA-PB2
Clone info. Influenza virus (A/PR/8/34) RNA polymerase PB2 gene inducible plasmid. Nucleotide sequence 5 to 2341nt of AF389115.1 (CDS, 28 to 2307nt) is cloned.
Comment Related cell line RCB1508 is available from RIKEN Cell Bank.
Vector backbone pBMSA (Plasmid)
Size of vector backbone 13.2 kb
Selectable markers Amp^r
Gene/insert name Influenza virus RNA polymerase PB2 cDNA
Depositor|Developer Nakada, Susumu |
Other clones in our bank
External Database
Influenza virus RNA polymerase PB2
          Reference sequence
            
           
          Remarks, protocol and/or map (gif) RDB01969.gif

          Distribution information

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          RDB01969 pBMSA-PB2 DNA solution

          Please review the QC test results indicated by check icon below as well as clone information before placing your order.

          How to cite this biological resource

          Materials & Methods section:

          The pBMSA-PB2 was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB01969).

          Reference section:

          Nakamura, Y., Oda, K., Nakada, S., Growth complementation of influenza virus temperature-sensitive mutants in mouse cells which express the RNA polymerase and nucleoprotein genes. J. Biochem., 110, 395-401 (1991). PMID 1663110.

          Further references such as user reports and related articles (go to bottom)

          References and tips

          Featured content

          QC test results

          RIKEN BRC has sequenced portions of this material for quality test.
          Please review the QC test results indicated by check icon as well as clone information before placing your order.

          Test sheet RDB15076_A7Eop1-2.pdf check

          Nucleotide sequence of a portion of this resource (if available).

          Primer: MMTV-LTR#2 ()
          Sequence file: RDB15076_A7Eoa.seq check
          >01969_15076_A7Eo_1_MMLV-LTR#2_C04_07_ABI24.ab1
              1 CGTGGGGGTT TGTGTCTGTT CGCCATCCCG TCTCCGCTCG TCACTTATCC TTCACTTTCC
             61 AGAGGGTCCC CCCGCAGACC CCGGCGACCC TCAGGTCGGC CGACTGCGGC AGCTCTAGAG
            121 GATCCCGAAT TCCAGCAAAA GCAGGTCAAT TATATTCAAT ATGGAAAGAA TAAAAGAACT
            181 AAGAAATCTA ATGTCGCAGT CTCGCACCCG CGAGATACTC ACAAAAACCA CCGTGGACCA
            241 TATGGCCATA ATCAAGAAGT ACACATCAGG AAGACAGGAG AAGAACCCAG CACTTAGGAT
            301 GAAATGGATG ATGGCAATGA AATATCCAAT TACAGCAGAC AAGAGGATAA CGGAAATGAT
            361 TCCTGAGAGA AATGAGCAAG GACAAACTTT ATGGAGTAAA ATGAATGATG CAGGATCAGA
            421 CCGAGTGATG GTATCACCTC TGGCTGTGAC ATGGTGGAAT AGGAATGGAC CAATAACAAA
            481 TACAGTTCAT TATCCAAAAA TCTACAAAAC TTATTTTGAA AGAGTCGAAA GGCTAAAGCA
            541 TGGAACCTTT GGCCCTGTCC ATTTTAGAAA CCAAGTCAAA ATACGTCGGA GAGTTGACAT
            601 AAATCCTGGT CATGCAGATC TCAGTGCCAA GGAGGCACAG GATGTAATCA TGGAAGTTGT
            661 TTTCCCTAAC GAAGTGGGAG CCAGGATACT AACATCGGAA TCGCAACTAA CGATAACCAA
            721 AGAGAAGAAA GAAGAACTCC AGGATTGCAA AATTTCTCCT TTGATGGTTG CATACATGTT
            781 GGAGAGAGAA CTGGTCCGCA AAACGAGATT CCTCCCAGTG GCTGGTGGAA CAAGCAGTGT
            841 GTACATTGAA GTGTTGCATT TGACTCAAGG AACATGCTGG GAACAGATGT ATACTCCAGG
            901 AGGGGAAGTG AGGAATGATG ATGTTGATCA AAGCTTGATT ATTGCTGCTA GGAACATAGT
            961 GAGAAGAGCT GCAGTATCAG CAGATCCACT AGCATCTTTT ATTTGGAGAT GTGCCACAGC
           1021 ACACAGATTG GGTGGGAAAT TAGGATGGTA GACATCCTTA GCAGAACCAC CAGAAGAGCA
           1081 AGCGTGGAAT ATATGCAGCC TGCCATGGGA CTGAGATTAG CTTCATCTCA GTTGTGATCA
          1141 CAAGTGGA
          //
          Primer: SV40NLS_R ()
          Sequence file: RDB15076_A7Eob.seq check
          >01969_15076_A7Eo_sv40NLS_R_B01_02_ABI08.ab1
              1 ATAAGGGAGT AACCAGGTAG CCTCATCATC ACTAGATGGC ATTTCTTCTG AGCAAAACAG
             61 GTTTTCCTCA TTAAAGGCAT TCCACCACTG CTCCCATTCA TCAGTTCCAT AGGTTGGAAT
            121 CTAAAATACA CAAACAATTA GAATCAGTAG TTTAACACAT TATACACTTA AAAATTTTAT
            181 ATTTACCTTA GAGCTTTAAA TCTCTGTAGG TAGTTTGTCC AATTATGTCA CACCACAGAA
            241 GTAAGGTTCC TTCACAAAGA TCCTCTAGAG GATCCCGAAT TCCAGTAGAA ACAAGGTCGT
            301 TTTTAAACTA TTCGACACTA ATTGATGGCC ATCCGAATTC TTTTGGTCGC TGTCTGGCTG
            361 TCAGTAAGTA TGCTAGAGTC CCGTTTCCGT TTCATTACCA ACACCACGTC TCCTTGCCCA
            421 ATTAGCACAT TAGCCTTCTC TCCTTTCGCA AGGTTGCTCA GTTCATTGAT GCTTAGTGCT
            481 GGCCCATATC TCTTGTCTTC TTTGCCCAGA ATGAGGAATC CCCTCAGAAC AGCGGACTCC
            541 ACTCCAGCTG TGCCTTCATC TGGGTCTTCA GTTAAAGTGC CAGCATCCTT TCCGAGAACT
            601 GTGAGTCTCT TCGTGGCCTT GTTATAGTTG AATACAGGAG AATTGCCCCT TACAAGTATT
            661 CTCATTCCTG ATCCCCTCAC ATTCACAGTA AATGAGGAGA ACTGCATTCT ACTTTGCTTT
            721 GGTGGAGCGG CTGCGAAGGG AAGAAGTTTT ATTATCTGTG CGGTATCAAA TGTCCCAAGC
            781 ACATCCCTCA TTTGTTGGGA ACAGAGTTCT TACAAACCCA CTGTATTGGG CCTCTAATGG
            841 CCTTAGGTAC TAAAAGACTG AAATGGTTTC AAATTCCATT TTATTGTATA GCATTGTAGG
            901 GTTCTGGGAC CACTGAAATT TTAACAGTTT CCCAGTTTCT GATGATCCAT TGATAGGTAT
            961 TGACCAACAC TGATTCAGGA CCATTAAATC TCCCCACAAT CAATTGACGA TGGAGTAAGG
           1021 TTATTGGTCA GTTTCCTCTG TTTCCTGGTG TTTTCAACTG GACCTCTTCG GAAGAACAGT
          1081 TAGTTAACAA TTTCTCGATG GTCCGAATTC TTCAAAAAAC CGGTCAGAAT GCTTTCA
          //
          Primer: pJB8-Forward ()
          Sequence file: RDB15076_A7Eoc.seq check
          >01969_15076_A7Eo_1_pJB8-Forward_C09_09_ABI24.ab1
              1 TGCCTGGACT TCCGTCCGCC AGGGGGACAT GCCGGCGATG CTGAAGGTCG CGCGCATTCC
             61 CGATGAAGAG GCCGGTTACC GCCTGTTGAC CTGGTGGGAC GGGCAGGGCG CCGCCCGAGT
            121 CTTCGCCTCG GCGGCGGGCG CTCTGCTCAT GGAGCGCGCG TCCGGGGCCG GGGACCTTGC
            181 ACAGATAGCG TGGTCCGGCC AGGACGACGA GGCTTGCAGG ATCATAATCA GCCATACCAC
            241 ATTTGTAGAG GTTTTACTTG CTTTAAAAAA CCTCCCACAC CTCCCCCTGA ACCTGAAACA
            301 TAAAATGAAT GCAATTGTTG TTGTTAACTT GTTTATTGCA GCTTATAATG GTTACAAATA
            361 AAGCAATAGC ATCACAAATT TCACAAATAA AGCATTTTTT TCACTGCATT CTAGTTGTGG
            421 TTTGTCCAAA CTCATCAATG TATCTTATCA TGTCTGGATC GATCCAGAGC TGTAACAGCA
            481 GGGACCCGCC CCAGTATAGG TGCGGGCATT CCTTTAGACA CCCTTGAAAC TCTTGGGGCC
            541 TTGCGTCCAG GGGTGTATGA GGACACTGTG CTACCAGAGG CCCCTGCAAT AGTCACTCCT
            601 GATGCTGTTC CTGCAGATTC AGGGCTTGAT GCCCTGTCCA TAGGTACAGA CTCGTCCACG
            661 GAGACCCTCA TTACTCTGCT AGAGCCTGAG GGTCCCGAGG ACATAGCGGT TCTTGAGCTG
            721 CAACCCCTGG ACCGTCCAAC TTGGCAAGTA AGCAATGCTG TTCATCAGTC CTCTGCATAC
            781 CACGCCCCTC TGCAGCTGCA ATCGTCCATT GCAGAAACAT CTGGTTTAGA AAATATTTTT
            841 GTAGGAGGCT CGGGTTTAGG GGATACAGGA GGAGAAAACA TTGAACTGAC ATACTTCGGG
            901 TCCCCACGAA CAAGCACGCC CCGCAGTATT GCCTCTAAAT CACGTGGCAT TTTAAACTGG
            961 TTCAGTAACG GTACTACACA CAGGTGCCCA CGGGAAGATC CTGAGTGTTT TTCATCCCAA
           1021 ACATTTGCAA ACCCACTGTT ATGAGCAGAT CAGCTGTGCT AGGACTAGTG GACGTGTGAA
           1081 CTCAGTCAGT TTATAACCTG ATACCTTACA ACCGTAGCAG GACCGAGGTG GACCCACAAG
          1141 CTACATGGTT CCAAGGTACT
          //
          Primer: BPV-1_F ()
          Sequence file: RDB15076_A7Eod.seq check
          >01969_15076_A7Eo_1_BPV-1_F_D04_10_ABI24.ab1
              1 CATGCCATGG TCTACTGGAG GGTTGCTGCA GGTGGATCAC CAAGGTACAC ACCACTCCGA
             61 ACAGCAGGGT CCACATCATC GCTTGCATCA ATAGGATCCA GACATGATAA GATACATTGA
            121 TGAGTTTGGA CAAACCACAA CTAGAATGCA GTGAAAAAAA TGCTTTATTT GTGAAATTTG
            181 TGATGCTATT GCTTTATTTG TAACCATTAT AAGCTGCAAT AAACAAGTTA ACAACAACAA
            241 TTGCATTCAT TTTATGTTTC AGGTTCAGGG GGAGGTGTGG GAGGTTTTTT AAAGCAAGTA
            301 AAACCTCTAC AAATGTGGTA TGGCTGATTA TGATCTCTAG TCAAGGCACT ATACATCAAA
            361 TATTCCTTAT TAACCCCTTT ACAAATTAAA AAGCTAAAGG TACACAATTT TTGAGCATAG
            421 TTATTAATAG CAGACACTCT ATGCCTGTGT GGAGTAAGAA AAAACAGTAT GTTATGATTA
            481 TAACTGTTAT GCCTACTTAT AAAGGTTACA GAATATTTTT CCATAATTTT CTTGTATAGC
            541 AGTGCAGCTT TTTCCTTTGT GGTGTAAATA GCAAAGCAAG CAAGAGTTCT ATTACTAAAC
            601 ACAGCATGAC TCAAAAAACT TAGCAAATTC TGAAGGAAAG TCCTTGGGGT CTTCTACCTT
            661 TCTCTTCTTT TTTGGAGGAG TAGAATGTTG AGAGTCAGCA GTAGCCTCAT CATCACTAGG
            721 ATGGCATTTT CTTCTGAGCA AAACAGGTTT TCCTCATTAA AAGGCATTCC ACCACTGCTC
          781 CCAATTCATC AGTTTCCATA GGTTGGGAAT CTAAATACAC AAGCAAATTA GAATCAGTA
          //

          Please visit Sequencing and PCR primers for primer information.

          References

          Original, user report and related articles

          original Nakamura, Y., Growth complementation of influenza virus temperature-sensitive mutants in mouse cells which express the RNA polymerase and nucleoprotein genes. J. Biochem., 110, 395-401 (1991). PMID 1663110.
          user_report Tsurumura, T., Crystallization and preliminary X-ray diffraction studies of a surface mutant of the middle domain of PB2 from human influenza A (H1N1) virus. Acta. Crystallogr. F Struct. Biol. Commun., 70 (Pt 1): 72-75 (2014). PMID 24419622.
          user_report Tsurumura, T., Conformational Polymorphism of m(7)GTP in Crystal Structure of the PB2 Middle Domain from Human Influenza A Virus. PLoS One, 8 (11): e82020 (2013). PMID 24312396.
          user_report Iwai Y., Anti-influenza activity of phenethylphenylphthalimide analogs derived from thalidomide. Bioorg. Med. Chem., 18, 5379-5390 (2010). PMID 20538468.
          user_report Kuzuhara T., Structural basis of the influenza A virus RNA polymerase PB2 RNA-binding domain containing the pathogenicity-determinant lysine 627 residue. J. Biol. Chem. 284, 6855-6860 (2009) PMID 19144639.
          user_report Kuzuhara T., Crystallization and X-ray diffraction analysis of the RNA primer/promoter-binding domain of influenza A virus RNA-dependent RNA polymerase PB2. Acta Crystallogr. Sect. F. Struct. Biol. Cryst. Commun., 65 (Pt 2): 144-146 (2009). PMID 19194006.

          2023.05.02

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