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pGMU20

E. coli - S. cerevisiae shuttle vector

Catalog number RDB01962
Resource name pGMU20
Alternative name E. coli-S. cerevisiae shuttle vector
Clone info. E. coli-S. cerevisiae shuttle vector, 9E10 epitope (Myc-tag), 2micron ori, URA3, GAL1-10 promoter, Ampicillin
Vector backbone pGMU20 (Plasmid)
Size of vector backbone 5,689 bp
Selectable markers Amp^r (E. coli), URA3 (S. cerevisiae)
Depositor|Developer Iha, Hidekatsu |
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External Database

            Reference sequence
              

            Distribution information

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            RDB01962 pGMU20 DNA solution Please contact us.

            Ordering Information [in Japanese] [in English]
            Please review the QC test results indicated by check icon below as well as clone information before placing your order.

            References and tips

            Electronic file

            Sequence RDB01962z.seq
            Remarks, protocol and/or map (gif) RDB01962.gif
            Remarks, protocol and/or map (gif) RDB01962z.gif

            Original reference

            original Iha, H., Shuttle-vector system for Saccharomyces cerevisiae designed to produce C-terminal-Myc-tagged fusion proteins. Biotechniques, 25, 936-938 (1998). PMID 9863043.

            Further references such as user reports and related articles (go to bottom)

            Featured content

            Featured content Expression Vector Backbone of Saccharomyces cerevisiae (English text)

            Sequence information

            RIKEN BRC has sequenced portions of this material for quality test.
            Please review the QC test results indicated by check icon as well as clone information before placing your order.

            Test sheet RDB12316_A5Bg.pdf check

            Nucleotide sequence of a portion of this resource (if available).

            Primer: ADH_rev
            Sequence file: RDB12316_A5Bga.seq check
            >01962_12316_A5Bg_ADHrev_D08_11.ab1
                1 TAAGATCTCA GAATAAGATT TTCGTTTTAA ACCTAAGAGT CACCCTCTAA AATTTGTATA
               61 CACTTATTTT TTTTATAACT TATTTAATAA TAAAAATCAT AAATCATAAG AAATTCGCCC
              121 GGAATTAGCT TGGCTGCAGG TCGACTCACA GATCCTCTTC GGAAATCAGT TTCTGTTCAG
              181 AATTCGAGCT CGGTACCCGG GGATCCTCTA GAGTCAATTC GATCCGGGGT TTTTTCTCCT
              241 TGACGTTAAA GTATAGAGGT ATATTAACAA TTTTTTGTTG ATACTTTTAT TACATTTGAA
              301 TAAGAAGTAA TACAAACCGA AAATGTTGAA AGTATTAGTT AAAGTGGTTA TGCAGTTTTT
              361 GCATTTATAT ATCTGTTAAT AGATCAAAAA TCATCGCTTC GCTGATTAAT TACCCCAGAA
              421 ATAAGGCTAA AAAACTAATC GCATTATCAT CCTATGGTTG TTAATTTGAT TCGTTCATTT
              481 GAAGGTTTGT GGGGCCAGGT TACTGCCAAT TTTTCCTCTT CATAACCATA AAAGCTAGTA
              541 TTGTAGAATC TTTATTGTTC GGAGCAGTGC GGCGCGAGGC ACATCTGCGT TTCAGGAACG
              601 CGACCGGTGA AGACGAGGAC GCACGGAGGA GAGTCTTCCT TCGGAGGGCT GTCACCCGCT
              661 CGGCGGCTTC TAATCCGTAC TTCAATATAG CAATGAGCAG TTAAGCGTAT TACTGAAAGT
              721 TCCAAAGAGA AGGTTTTTTT AGGCTAAGAT AATGGGGCTC TTTACATTTC CACAACATAT
              781 AAGTAAGATT AGATATGGAT ATGTATATGG ATATGTATAT GGTGGTAATG CCATGTAATA
              841 TGATTATTAA ACTTCTTTGC GTCCATCCAA AAAAAAAGTA AGAATTTTTT GAAAATTCAA
              901 TATAAATGAC AGCTCAGTTA CAAAGTGAAA GTGACCTGCA GGCATGCAAG CAACGAAGCA
              961 TCTGTGCTTC ATTTTGTAGA ACAAAAATGC AACGCGAGAG CGCTAAATTT TTCAAACAAA
             1021 GAATCTGAGC TGCATTTTTT TACAGACAGA AATGCACGCG GAAGCGCTAT TTTACAACGA
            1081 AGAATCTGTG CTCAATTTTT GGTAAAACCA AAAAAGTTGC AACCGCCG
            //
            Primer: Amp_R
            Sequence file: RDB12316_A5Bgb.seq check
            >01962_12316_A5Bg_Amp_R_E08_14.ab1
                1 TCTATTTTCA TATTATTGAG CATTTATCAG GGTTATTGTC TCATGAGCGG ATACATATTT
               61 GAATGTATTT AGAAAAATAA ACAAATAGGG GTTCCGCGCA CATTTCCCCG AAAAGTGCCA
              121 CCTGACGTCT AAGAAACCAT TATTATCATG ACATTAACCT ATAAAAATAG GCGTATCACG
              181 AGGCCCTTTC GTCTCGCGCG TTTCGGTGAT GACGGTGAAA ACCTCTGACA CATGCAGCTC
              241 CCGGAGACGG TCACAGCTTG TCTGTAAGCG GATGCCGGGA GCAGACAAGC CCGTCAGGGC
              301 GCGTCAGCGG GTGTTGGCGG GTGTCGGGGC TGGCTTAACT ATGCGGCATC AGAGCAGATT
              361 GTACTGAGAG TGCACCATAA CGCATTTAAG CATAAACACG CACTATGCCG TTCTTCTCAT
              421 GTATATATAT ATACAGGCAA CACGCAGATA TAGGTGCGAC GTGAACAGTG AGCTGTATGT
              481 GCGCAGCTCG CGTTGCATTT TCGGAAGCGC TCGTTTTCGG AAACGCTTTG AAGTTCCTAT
              541 TCCGAAGTTC CTATTCTCTA GCTAGAAAGT ATAGGAACTT CAGAGCGCTT TTGAAAACCA
              601 AAAGCGCTCT GAAGACGCAC TTTCAAAAAA CCAAAAACGC ACCGGACTGT AACGAGCTAC
              661 TAAAATATTG CGAATACCGC TTCCACAAAC ATTGCTCAAA AGTATCTCTT TGCTATATAT
              721 CTCTGTGCTA TATCCCTATA TAACCTACCC ATCCACCTTT CGCTCCTTGA ACTTGCATCT
              781 AAACTCGACC TCTACATTTT TTATGTTTAT CTCTAGTATT ACTCTTTAGA CAAAAAAAAT
              841 TGTAGTAAGA ACTATTCATA GAGTGAATCG AAAACAATAC GAAAAATGTA AACATTTCCT
              901 ATACGTAGTA TATAGAGACA AAATAGAAGA AACCGTTCAT AATTTTCTGA CCAATGAAGA
              961 ATCATCAACG CTATCACTTT CTGTTCACAA AGTATGCGCA ATCCACATCG GTATAGAAAT
             1021 ATAAATCGGG GATGCCCTTT ATCTGAAAAA ATGCACCCCG CAGCCTTCGC CTAGTAATCA
             1081 GTAAACCGCG GGCAGTTGCA AGTTCAAGCC TTTTTTTATG GCCAGCAGCA AAAAATTAGA
            1141 CCACCCAAAA AAAG
            //
            Primer: URA3pro_F
            Sequence file: RDB12316_A5Bgc.seq check
            >01962_12316_A5Bg_URA3pro_F_F08_17.ab1
                1 CAATGCTGTG CTGAGACATG AATTGCCCAG TATTCTTAAC CCAACTGCAC AGAACAAAAA
               61 CCTGCAGGAA ACGAAGATAA ATCATGTCGA AAGCTACATA TAAGGAACGT GCTGCTACTC
              121 ATCCTAGTCC TGTTGCTGCC AAGCTATTTA ATATCATGCA CGAAAAGCAA ACAAACTTGT
              181 GTGCTTCATT GGATGTTCGT ACCACCAAGG AATTACTGGA GTTAGTTGAA GCATTAGGTC
              241 CCAAAATTTG TTTACTAAAA ACACATGTGG ATATCTTGAC TGATTTTTCC ATGGAGGGCA
              301 CAGTTAAGCC GCTAAAGGCA TTATCCGCCA AGTACAATTT TTTACTCTTC GAAGACAGAA
              361 AATTTGCTGA CATTGGTAAT ACAGTCAAAT TGCAGTACTC TGCGGGTGTA TACAGAATAG
              421 CAGAATGGGC AGACATTACG AATGCACACG GTGTGGTGGG CCCAGGTATT GTTAGCGGTT
              481 TGAAGCAGGC GGCAGAAGAA GTAACAAAGG AACCTAGAGG CCTTTTGATG TTAGCAGAAT
              541 TGTCATGCAA GGGCTCCCTA TCTACTGGAG AATATACTAA GGGTACTGTT GACATTGCGA
              601 AGAGCGACAA AGATTTTGTT ATCGGCTTTA TTGCTCAAAG AGACATGGGT GGAAGAGATG
              661 AAGGTTACGA TTGGTTGATT ATGACACCCG GTGTGGGTTT AGATGACAAG GGAGACGCAT
              721 TGGGTCAACA GTATAGAACC GTGGATGATG TGGTCTCTAC AGGATCTGAC ATTATTATTG
              781 TTGGAAGAGG ACTATTTGCA AAGGGAAGGG ATGCTAAGGT AGAGGGTGAA CGTTACAGAA
              841 AAGCAGGCTG GGAAGCATAT TTGAGAAGAT GCGGCCAGCA AAACTAAAAA AACTGTATTA
              901 TAAGTAAAAG CTTACATTTT ATGTTAGCTG GTGGACTGAC GCCAGAAAAT GTTGGTGATG
              961 CGCTTAGATT AAATGGGCGT TTATTGGTGT TTGATGTAAG CGGGAGGTGT GGAGACAAAT
             1021 GGTGTAAAAG ACTCTACAAA ATAGCAATTT CGTCAAAAAT GCTAGAAATA GTATTACTGA
            1081 GTAGTATTAT TAGGTATTGA TTGTTGCACT TGTCCGGCTA GAAGGGTG
            //

            Please visit Sequencing and PCR primers for primer information.


            References

            Original, user report and related articles

            original Iha, H., Shuttle-vector system for Saccharomyces cerevisiae designed to produce C-terminal-Myc-tagged fusion proteins. Biotechniques, 25, 936-938 (1998). PMID 9863043.

            2022.02.09

            GNP_filter3_RDBDEP_html_220112.pl