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pGMU10

E. coli - S. cerevisiae shuttle vector

Catalog number RDB01961
Resource name pGMU10
Alternative name E. coli-S. cerevisiae shuttle vector
Clone info. E. coli-S. cerevisiae shuttle vector, 9E10 epitope (Myc-tag), centromere-type ori, URA3, GAL1-10 promoter, Ampicillin
Vector backbone pGMU10 (Plasmid)
Size of vector backbone 4,897 bp
Selectable markers Amp^r (E. coli), URA3 (S. cerevisiae)
Depositor Iha, Hidekatsu |

Distribution information

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RDB01961 pGMU10 DNA solution Please contact us.

Ordering Information [in Japanese] [in English]

References and tips

Electronic file

Electronic file Sequence RDB01961z.seq from Depositor
Electronic file Map RDB01961.gif from Depositor
Electronic file Map RDB01961z.gif from Depositor

Featured content

Featured content Expression Vector Backbone of Saccharomyces cerevisiae (English text)

References

original Iha, H., Shuttle-vector system for Saccharomyces cerevisiae designed to produce C-terminal-Myc-tagged fusion proteins. Biotechniques, 25, 936-938 (1998). PMID 9863043.

User reports and related articles (go to bottom)


Sequence information

RIKEN BRC has sequenced portions of this material for quality test. Primers and the results are shown below.

Data are summerized on test sheet below.

Test sheet RDB13047_A4Ljp1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: ADH_rev RDB13047_A4Lja.seq
>01961_13047_A4Lj_1_ADHrev_A03_03.ab1
    1 TAGGGAGTTC TTCAAGGAAT AAAGAAGGTT TCGTTTTAAA ACCTAAGAGT CATTTTTAAA
   61 ATTTGTATAC ACTTATTTTT TTTATAACTT ATTTAATAAT AAAAATCATA AATCATAAGA
  121 AATTCGCCCG GAATTAGCTT GGCTGCAGGT CGACTCACAG ATCCTCTTCG GAAATCAGTT
  181 TCTGTTCAGA ATTCGAGCTC GGTACCCGGG GATCCTCTAG AGTCAATTCG ATCCGGGGTT
  241 TTTTCTCCTT GACGTTAAAG TATAGAGGTA TATTAACAAT TTTTTGTTGA TACTTTTATT
  301 ACATTTGAAT AAGAAGTAAT ACAAACCGAA AATGTTGAAA GTATTAGTTA AAGTGGTTAT
  361 GCAGTTTTTG CATTTATATA TCTGTTAATA GATCAAAAAT CATCGCTTCG CTGATTAATT
  421 ACCCCAGAAA TAAGGCTAAA AAACTAATCG CATTATCATC CTATGGTTGT TAATTTGATT
  481 CGTTCATTTG AAGGTTTGTG GGGCCAGGTT ACTGCCAATT TTTCCTCTTC ATAACCATAA
  541 AAGCTAGTAT TGTAGAATCT TTATTGTTCG GAGCAGTGCG GCGCGAGGCA CATCTGCGTT
  601 TCAGGAACGC GACCGGTGAA GACGAGGACG CACGGAGGAG AGTCTTCCTT CGGAGGGCTG
  661 TCACCCGCTC GGCGGCTTCT AATCCGTACT TCAATATAGC AATGAGCAGT TAAGCGTATT
  721 ACTGAAAGTT CCAAAGAGAA GGTTTTTTTA GGCTAAGATA ATGGGGCTCT TTACATTTCC
  781 ACAACATATA AGTAAGATTA GATATGGATA TGTATATGGA TATGTATATG GTGGTAATGC
  841 CATGTAATAT GATTATTAAA CTTCTTTGCG TCCATCCAAA AAAAAAGTAA GAATTTTTGA
  901 AAATTCAATA TAAATGACAG CTCAGTTTAC AAAGTGAAAG TGACCTGCAG GCATGCAAGC
  961 TCTGCATGTG TCAGAGGTTT TCACCGTCAT CACCGAAACG CGCGAGACGA AAGGGCCTCG
 1021 TGATACGCCT ATTTTTTATA GGTTAATGTC AATGATAATA ATGGTTTTCT TAGGTTTCTT
 1081 TTTCATCACG TGCTATAAAA AATAATTATT AAATTTAAGT TTTTTAAGTA TCAATATATT
1141 CAAACTTACA AATAGGAAAG TAAAAAAAGG AAATTTTA
//
Primer: Amp_R RDB13047_A4Ljb.seq
>01961_13047_A4Lj_1_Amp_R_B01_04.ab1
    1 TCCCTTTTTA AAATTATTGA GCATTTATCA GGGTTATTGT CTCATGCAGC GGATACATAT
   61 TTGAATGTAT TTAGAAAAAT AAACAAATAG GGGTTCCGCG CACATTTCCC CGAAAAGTGC
  121 CACCTGACGG ATCGCTTGCC TGTAACTTAC ACGCGCCTCG TATCTTTTAA TGATGGAATA
  181 ATTTGGGAAT TTACTCTGTG TTTATTTATT TTTATGTTTT GTATTTGGAT TTTAGAAAGT
  241 AAATAAAGAA GGTAGAAGAG TTACGGAATG AAGAAAAAAA AATAAACAAA GGTTTAAAAA
  301 ATTTCAACAA AAAGCGTACT TTACATATAT ATTTATTAGA CAAGAAAAGC AGATTAAATA
  361 GATATACATT CGATTAACGA TAAGTAAAAT GTAAAATCAC AGGATTTTCG TGTGTGGTCT
  421 TCTACACAGA CAAGATGAAA CAATTCGGCA TTAATACCTG AGAGCAGGAA GAGCAAGATA
  481 AAAGGTAGTA TTTGTTGGCG ATCCCCCTAG AGTCTTTTAC ATCTTCGGAA AACAAAAACT
  541 ATTTTTTCTT TAATTTCTTT TTTTACTTTC TATTTTTAAT TTATATATTT ATATTAAAAA
  601 ATTTAAATTA TAATTATTTT TATAGCACGT GATGAAAAGG ACCCTAAGAA ACCATTATTA
  661 TCATGACATT AACCTATAAA AATAGGCGTA TCACGAGGCC CTTTCGTCTC GCGCGTTTCG
  721 GTGATGACGG TGAAAACCTC TGACACATGC AGAGCTTGCA TGCCTGCAGG TCACTTTCAC
  781 TTTGTAACTG AGCTGTCATT TATATTGAAT TTTCAAAAAT TCTTACTTTT TTTTTGGATG
  841 GACGCAAAGA AGTTTAATAA TCATATTACA TGGCATTACC ACCATATACA TATCCATATA
  901 CATATCCATA TCTAATCTTA CTTATATGTT GTGGAAATGT AAAGAGCCCC ATTATCTTAG
  961 CCTAAAAAAA ACCTTCTCTT TGGAACTTTT CAGTAATACG CTTAAACTGC TTCATTGCTA
 1021 TATTGAAGTT ACGGAATTAG AAAGCCCGCG AGCGGGTTGA CAGCCCCTTC CGAAAGGAGA
 1081 CTCTCTTCCG TTGCGTCCTC GTCTTCAACT GATCGCGATT CCCTGAAACG CCAGAATGTT
1141 GCCCTTTCGC GGCTCGTCAA CATGTGC
//
Primer: URA3pro_F RDB13047_A4Ljc.seq
>01961_13047_A4Lj_1_URA3pro_F_E01_13.ab1
    1 CCATTGTAGT GTTGAAGGAA CATGAATTGC CCAGTATTCT TAACCCAACT GCACAGAACA
   61 AAAACCTGCA GGAAACGAAG ATAAATCATG TCGAAAGCTA CATATAAGGA ACGTGCTGCT
  121 ACTCATCCTA GTCCTGTTGC TGCCAAGCTA TTTAATATCA TGCACGAAAA GCAAACAAAC
  181 TTGTGTGCTT CATTGGATGT TCGTACCACC AAGGAATTAC TGGAGTTAGT TGAAGCATTA
  241 GGTCCCAAAA TTTGTTTACT AAAAACACAT GTGGATATCT TGACTGATTT TTCCATGGAG
  301 GGCACAGTTA AGCCGCTAAA GGCATTATCC GCCAAGTACA ATTTTTTACT CTTCGAAGAC
  361 AGAAAATTTG CTGACATTGG TAATACAGTC AAATTGCAGT ACTCTGCGGG TGTATACAGA
  421 ATAGCAGAAT GGGCAGACAT TACGAATGCA CACGGTGTGG TGGGCCCAGG TATTGTTAGC
  481 GGTTTGAAGC AGGCGGCAGA AGAAGTAACA AAGGAACCTA GAGGCCTTTT GATGTTAGCA
  541 GAATTGTCAT GCAAGGGCTC CCTATCTACT GGAGAATATA CTAAGGGTAC TGTTGACATT
  601 GCGAAGAGCG ACAAAGATTT TGTTATCGGC TTTATTGCTC AAAGAGACAT GGGTGGAAGA
  661 GATGAAGGTT ACGATTGGTT GATTATGACA CCCGGTGTGG GTTTAGATGA CAAGGGAGAC
  721 GCATTGGGTC AACAGTATAG AACCGTGGAT GATGTGGTCT CTACAGGATC TGACATTATT
  781 ATTGTTGGAA GAGGACTATT TGCAAAGGGA AGGGATGCTA AGGTAGAGGG TGAACGTTAC
  841 AGAAAAGCAG GCTGGGAAGC ATATTTGAGA AGATGCGGCC AGCAAAACTA AAAAACTGTA
  901 TTATAAGTAA AAGCTTACAT TTTATGTTAG CTGGTGGACT GACGCCAGAA AATGTTGGTG
  961 ATGCGCTTAG ATTAAATGGC GTTATTGGTG TTGATGTAAG CGGAGGTGTG GAGACAAATG
 1021 GGTGTAAAAG ACTCTAACAA AATAGCAAAT TTCGTCAAAA ATGCTAAGAA AATAGGTTAT
 1081 TTACTGAAGT AGTATTTTTT ATTTTTAGTA ATTGGTTTGT GCAGTTGCCG TAGAGGTGTG
1141 GATCCATAGA GACGACCTTC ATTGCCTTTA ATATACCCCT TG
//

Please visit Sequencing and PCR primers for primer information.


References

References

original Iha, H., Shuttle-vector system for Saccharomyces cerevisiae designed to produce C-terminal-Myc-tagged fusion proteins. Biotechniques, 25, 936-938 (1998). PMID 9863043.

2018.12.10

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