Resource data sheet
Prev.
Please review the QC test results indicated by check icon below as well as clone information before placing your order.

pGMU10

E. coli - S. cerevisiae shuttle vector

Catalog number RDB01961
Resource name pGMU10
Alternative name E. coli-S. cerevisiae shuttle vector
Clone info. E. coli-S. cerevisiae shuttle vector, 9E10 epitope (Myc-tag), centromere-type ori, URA3, GAL1-10 promoter, Ampicillin
Vector backbone pGMU10 (Plasmid)
Size of vector backbone 4,897 bp
Selectable markers Amp^r (E. coli), URA3 (S. cerevisiae)
Depositor|Developer Iha, Hidekatsu |
Other clones in our bank

External Database

            Reference sequence
              

            Distribution information

            Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
            Terms and conditions set forth by the DEPOSITOR Acknowledgement to Depositor and Developer in publication.
            Ordering Please visit Information of Request for Distribution.[link] 
            Order form 
            [Credit Card Payment]  [Bank Transfer Payment]
            Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word]
            提供案内 (日本国内) [open/close]

            提供条件 研究結果の発表時、謝辞に寄託者および樹立者名を入れること。
            提供依頼 手続きの詳細は、「提供申込みについて[link]」をご覧ください。
            提供依頼書 [Word]
            提供同意書 (MTA、非営利機関による非営利学術研究用)[Word]

            Catalog # Resource name Shipping form Fee (non-profit org.)
            RDB01961 pGMU10 DNA solution Please contact us.

            Ordering Information [in Japanese] [in English]
            Please review the QC test results indicated by check icon below as well as clone information before placing your order.

            References and tips

            Electronic file

            Sequence RDB01961z.seq
            Remarks, protocol and/or map (gif) RDB01961.gif
            Remarks, protocol and/or map (gif) RDB01961z.gif

            Original reference

            original Iha, H., Shuttle-vector system for Saccharomyces cerevisiae designed to produce C-terminal-Myc-tagged fusion proteins. Biotechniques, 25, 936-938 (1998). PMID 9863043.

            Further references such as user reports and related articles (go to bottom)

            Featured content

            Featured content Expression Vector Backbone of Saccharomyces cerevisiae (English text)

            Sequence information

            RIKEN BRC has sequenced portions of this material for quality test.
            Please review the QC test results indicated by check icon as well as clone information before placing your order.

            Test sheet RDB13047_A4Ljp1.pdf check

            Nucleotide sequence of a portion of this resource (if available).

            Primer: ADH_rev
            Sequence file: RDB13047_A4Lja.seq check
            >01961_13047_A4Lj_1_ADHrev_A03_03.ab1
                1 TAGGGAGTTC TTCAAGGAAT AAAGAAGGTT TCGTTTTAAA ACCTAAGAGT CATTTTTAAA
               61 ATTTGTATAC ACTTATTTTT TTTATAACTT ATTTAATAAT AAAAATCATA AATCATAAGA
              121 AATTCGCCCG GAATTAGCTT GGCTGCAGGT CGACTCACAG ATCCTCTTCG GAAATCAGTT
              181 TCTGTTCAGA ATTCGAGCTC GGTACCCGGG GATCCTCTAG AGTCAATTCG ATCCGGGGTT
              241 TTTTCTCCTT GACGTTAAAG TATAGAGGTA TATTAACAAT TTTTTGTTGA TACTTTTATT
              301 ACATTTGAAT AAGAAGTAAT ACAAACCGAA AATGTTGAAA GTATTAGTTA AAGTGGTTAT
              361 GCAGTTTTTG CATTTATATA TCTGTTAATA GATCAAAAAT CATCGCTTCG CTGATTAATT
              421 ACCCCAGAAA TAAGGCTAAA AAACTAATCG CATTATCATC CTATGGTTGT TAATTTGATT
              481 CGTTCATTTG AAGGTTTGTG GGGCCAGGTT ACTGCCAATT TTTCCTCTTC ATAACCATAA
              541 AAGCTAGTAT TGTAGAATCT TTATTGTTCG GAGCAGTGCG GCGCGAGGCA CATCTGCGTT
              601 TCAGGAACGC GACCGGTGAA GACGAGGACG CACGGAGGAG AGTCTTCCTT CGGAGGGCTG
              661 TCACCCGCTC GGCGGCTTCT AATCCGTACT TCAATATAGC AATGAGCAGT TAAGCGTATT
              721 ACTGAAAGTT CCAAAGAGAA GGTTTTTTTA GGCTAAGATA ATGGGGCTCT TTACATTTCC
              781 ACAACATATA AGTAAGATTA GATATGGATA TGTATATGGA TATGTATATG GTGGTAATGC
              841 CATGTAATAT GATTATTAAA CTTCTTTGCG TCCATCCAAA AAAAAAGTAA GAATTTTTGA
              901 AAATTCAATA TAAATGACAG CTCAGTTTAC AAAGTGAAAG TGACCTGCAG GCATGCAAGC
              961 TCTGCATGTG TCAGAGGTTT TCACCGTCAT CACCGAAACG CGCGAGACGA AAGGGCCTCG
             1021 TGATACGCCT ATTTTTTATA GGTTAATGTC AATGATAATA ATGGTTTTCT TAGGTTTCTT
             1081 TTTCATCACG TGCTATAAAA AATAATTATT AAATTTAAGT TTTTTAAGTA TCAATATATT
            1141 CAAACTTACA AATAGGAAAG TAAAAAAAGG AAATTTTA
            //
            Primer: Amp_R
            Sequence file: RDB13047_A4Ljb.seq check
            >01961_13047_A4Lj_1_Amp_R_B01_04.ab1
                1 TCCCTTTTTA AAATTATTGA GCATTTATCA GGGTTATTGT CTCATGCAGC GGATACATAT
               61 TTGAATGTAT TTAGAAAAAT AAACAAATAG GGGTTCCGCG CACATTTCCC CGAAAAGTGC
              121 CACCTGACGG ATCGCTTGCC TGTAACTTAC ACGCGCCTCG TATCTTTTAA TGATGGAATA
              181 ATTTGGGAAT TTACTCTGTG TTTATTTATT TTTATGTTTT GTATTTGGAT TTTAGAAAGT
              241 AAATAAAGAA GGTAGAAGAG TTACGGAATG AAGAAAAAAA AATAAACAAA GGTTTAAAAA
              301 ATTTCAACAA AAAGCGTACT TTACATATAT ATTTATTAGA CAAGAAAAGC AGATTAAATA
              361 GATATACATT CGATTAACGA TAAGTAAAAT GTAAAATCAC AGGATTTTCG TGTGTGGTCT
              421 TCTACACAGA CAAGATGAAA CAATTCGGCA TTAATACCTG AGAGCAGGAA GAGCAAGATA
              481 AAAGGTAGTA TTTGTTGGCG ATCCCCCTAG AGTCTTTTAC ATCTTCGGAA AACAAAAACT
              541 ATTTTTTCTT TAATTTCTTT TTTTACTTTC TATTTTTAAT TTATATATTT ATATTAAAAA
              601 ATTTAAATTA TAATTATTTT TATAGCACGT GATGAAAAGG ACCCTAAGAA ACCATTATTA
              661 TCATGACATT AACCTATAAA AATAGGCGTA TCACGAGGCC CTTTCGTCTC GCGCGTTTCG
              721 GTGATGACGG TGAAAACCTC TGACACATGC AGAGCTTGCA TGCCTGCAGG TCACTTTCAC
              781 TTTGTAACTG AGCTGTCATT TATATTGAAT TTTCAAAAAT TCTTACTTTT TTTTTGGATG
              841 GACGCAAAGA AGTTTAATAA TCATATTACA TGGCATTACC ACCATATACA TATCCATATA
              901 CATATCCATA TCTAATCTTA CTTATATGTT GTGGAAATGT AAAGAGCCCC ATTATCTTAG
              961 CCTAAAAAAA ACCTTCTCTT TGGAACTTTT CAGTAATACG CTTAAACTGC TTCATTGCTA
             1021 TATTGAAGTT ACGGAATTAG AAAGCCCGCG AGCGGGTTGA CAGCCCCTTC CGAAAGGAGA
             1081 CTCTCTTCCG TTGCGTCCTC GTCTTCAACT GATCGCGATT CCCTGAAACG CCAGAATGTT
            1141 GCCCTTTCGC GGCTCGTCAA CATGTGC
            //
            Primer: URA3pro_F
            Sequence file: RDB13047_A4Ljc.seq check
            >01961_13047_A4Lj_1_URA3pro_F_E01_13.ab1
                1 CCATTGTAGT GTTGAAGGAA CATGAATTGC CCAGTATTCT TAACCCAACT GCACAGAACA
               61 AAAACCTGCA GGAAACGAAG ATAAATCATG TCGAAAGCTA CATATAAGGA ACGTGCTGCT
              121 ACTCATCCTA GTCCTGTTGC TGCCAAGCTA TTTAATATCA TGCACGAAAA GCAAACAAAC
              181 TTGTGTGCTT CATTGGATGT TCGTACCACC AAGGAATTAC TGGAGTTAGT TGAAGCATTA
              241 GGTCCCAAAA TTTGTTTACT AAAAACACAT GTGGATATCT TGACTGATTT TTCCATGGAG
              301 GGCACAGTTA AGCCGCTAAA GGCATTATCC GCCAAGTACA ATTTTTTACT CTTCGAAGAC
              361 AGAAAATTTG CTGACATTGG TAATACAGTC AAATTGCAGT ACTCTGCGGG TGTATACAGA
              421 ATAGCAGAAT GGGCAGACAT TACGAATGCA CACGGTGTGG TGGGCCCAGG TATTGTTAGC
              481 GGTTTGAAGC AGGCGGCAGA AGAAGTAACA AAGGAACCTA GAGGCCTTTT GATGTTAGCA
              541 GAATTGTCAT GCAAGGGCTC CCTATCTACT GGAGAATATA CTAAGGGTAC TGTTGACATT
              601 GCGAAGAGCG ACAAAGATTT TGTTATCGGC TTTATTGCTC AAAGAGACAT GGGTGGAAGA
              661 GATGAAGGTT ACGATTGGTT GATTATGACA CCCGGTGTGG GTTTAGATGA CAAGGGAGAC
              721 GCATTGGGTC AACAGTATAG AACCGTGGAT GATGTGGTCT CTACAGGATC TGACATTATT
              781 ATTGTTGGAA GAGGACTATT TGCAAAGGGA AGGGATGCTA AGGTAGAGGG TGAACGTTAC
              841 AGAAAAGCAG GCTGGGAAGC ATATTTGAGA AGATGCGGCC AGCAAAACTA AAAAACTGTA
              901 TTATAAGTAA AAGCTTACAT TTTATGTTAG CTGGTGGACT GACGCCAGAA AATGTTGGTG
              961 ATGCGCTTAG ATTAAATGGC GTTATTGGTG TTGATGTAAG CGGAGGTGTG GAGACAAATG
             1021 GGTGTAAAAG ACTCTAACAA AATAGCAAAT TTCGTCAAAA ATGCTAAGAA AATAGGTTAT
             1081 TTACTGAAGT AGTATTTTTT ATTTTTAGTA ATTGGTTTGT GCAGTTGCCG TAGAGGTGTG
            1141 GATCCATAGA GACGACCTTC ATTGCCTTTA ATATACCCCT TG
            //

            Please visit Sequencing and PCR primers for primer information.


            References

            Original, user report and related articles

            original Iha, H., Shuttle-vector system for Saccharomyces cerevisiae designed to produce C-terminal-Myc-tagged fusion proteins. Biotechniques, 25, 936-938 (1998). PMID 9863043.

            2022.02.09

            GNP_filter3_RDBDEP_html_220112.pl