Please review the QC test results indicated by icon below as well as clone information before placing your order.

pGMT10

E. coli - S. cerevisiae shuttle vector

Sequence	RDB01959z.seq
Remarks, protocol and/or map (gif)	RDB01959.gif
Remarks, protocol and/or map (gif)	RDB01959z.gif

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Please review the QC test results indicated by icon below as well as clone information before placing your order.

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QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by icon as well as clone information before placing your order.

Test sheet	RDB13046_A4Lj.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: ADHrev Sequence file: RDB13046_A4Lja.seq
>01959_13046_A4Lj_ADHrev_C10_07.ab1     1 TCAGATCTCA AGATAAGAAT TTTCGTTTTA AAACCTAAGA GTCACTTTAA AATTTGTATA    61 CACTTATTTT TTTTATAACT TATTTAATAA TAAAAATCAT AAATCATAAG AAATTCGCCC   121 GGAATTAGCT TGGCTGCAGG TCGACTCACA GATCCTCTTC GGAAATCAGT TTCTGTTCAG   181 AATTCGAGCT CGGTACCCGG GGATCCTCTA GAGTCAATTC GATCCGGGGT TTTTTCTCCT   241 TGACGTTAAA GTATAGAGGT ATATTAACAA TTTTTTGTTG ATACTTTTAT TACATTTGAA   301 TAAGAAGTAA TACAAACCGA AAATGTTGAA AGTATTAGTT AAAGTGGTTA TGCAGTTTTT   361 GCATTTATAT ATCTGTTAAT AGATCAAAAA TCATCGCTTC GCTGATTAAT TACCCCAGAA   421 ATAAGGCTAA AAAACTAATC GCATTATCAT CCTATGGTTG TTAATTTGAT TCGTTCATTT   481 GAAGGTTTGT GGGGCCAGGT TACTGCCAAT TTTTCCTCTT CATAACCATA AAAGCTAGTA   541 TTGTAGAATC TTTATTGTTC GGAGCAGTGC GGCGCGAGGC ACATCTGCGT TTCAGGAACG   601 CGACCGGTGA AGACGAGGAC GCACGGAGGA GAGTCTTCCT TCGGAGGGCT GTCACCCGCT   661 CGGCGGCTTC TAATCCGTAC TTCAATATAG CAATGAGCAG TTAAGCGTAT TACTGAAAGT   721 TCCAAAGAGA AGGTTTTTTT AGGCTAAGAT AATGGGGCTC TTTACATTTC CACAACATAT   781 AAGTAAGATT AGATATGGAT ATGTATATGG ATATGTATAT GGTGGTAATG CCATGTAATA   841 TGATTATTAA ACTTCTTTGC GTCCATCCAA AAAAAAAGTA AGAATTTTTT GAAAATTCAA   901 TATAAATGAC AGCTCAGTTA CAAAGTGAAA GTGACCTGCA GGCATGCAAG CTCTGCATGT   961 GTCAGAGGTT TTCACCGTCA TCACCGAAAC GCGCGAGACG AAAGGGCCTC GTGATACGCC  1021 TATTTTTTTA TAGGTTAATG TCATGATAAT AATGGATTTT CTTACGGTCG TTTTTCAATC  1081 ACGTGCTAAT GAAATAATAT AGTTGAAGCT TTTTTAATAT AACTATTATA AGTTTAAAAA 1141 AA //
Primer: Amp_R Sequence file: RDB13046_A4Ljb.seq
>01959_13046_A4Lj_Amp_R_D10_10.ab1     1 ACTTTTTTCA TATTTATTGA GCATTTATCA GGGTTATTGT CTCATGCAGC GGATACATAT    61 TTGAATGTAT TTAGAAAAAT AAACAAATAG GGGTTCCGCG CACATTTCCC CGAAAAGTGC   121 CACCTGACGG ATCGCTTGCC TGTAACTTAC ACGCGCCTCG TATCTTTTAA TGATGGAATA   181 ATTTGGGAAT TTACTCTGTG TTTATTTATT TTTATGTTTT GTATTTGGAT TTTAGAAAGT   241 AAATAAAGAA GGTAGAAGAG TTACGGAATG AAGAAAAAAA AATAAACAAA GGTTTAAAAA   301 ATTTCAACAA AAAGCGTACT TTACATATAT ATTTATTAGA CAAGAAAAGC AGATTAAATA   361 GATATACATT CGATTAACGA TAAGTAAAAT GTAAAATCAC AGGATTTTCG TGTGTGGTCT   421 TCTACACAGA CAAGATGAAA CAATTCGGCA TTAATACCTG AGAGCAGGAA GAGCAAGATA   481 AAAGGTAGTA TTTGTTGGCG ATCCCCCTAG AGTCTTTTAC ATCTTCGGAA AACAAAAACT   541 ATTTTTTCTT TAATTTCTTT TTTTACTTTC TATTTTTAAT TTATATATTT ATATTAAAAA   601 ATTTAAATTA TAATTATTTT TATAGCACGT GATGAAAAGG ACCCTAAGAA ACCATTATTA   661 TCATGACATT AACCTATAAA AATAGGCGTA TCACGAGGCC CTTTCGTCTC GCGCGTTTCG   721 GTGATGACGG TGAAAACCTC TGACACATGC AGAGCTTGCA TGCCTGCAGG TCACTTTCAC   781 TTTGTAACTG AGCTGTCATT TATATTGAAT TTTCAAAAAT TCTTACTTTT TTTTTGGATG   841 GACGCAAAGA AGTTTAATAA TCATATTACA TGGCATTACC ACCATATACA TATCCATATA   901 CATATCCATA TCTAATCTTA CTTATATGTT GTGGAAATGT AAAGAGCCCC ATTATCTTAG   961 CCTAAAAAAA ACCTTCTCTT TGGAAACTTT CAGTAATACG CTTAAACTGC TCATTGCTAT  1021 ATTTGAAAGT ACGGATTAGA AGCCCGCCCG AGCGGGTGAC AGCCCCTCCG AAGGAGACTC  1081 TCCTCCGTGC GTCCTCGTCC TTCAACGGAT CGCGATTCCT GAAAACGGCC AGAAATTGTT 1141 TGCCCTTTTC GTCAGC //
Primer: TRP1pro_F Sequence file: RDB13046_A4Ljc.seq
>01959_13046_A4Lj_TRP1pro_F_E10_13.ab1     1 GCTAAGCTAT AGCCACAAAG GCAGCTTGGA GTATGTCTGT TATTAATTTC ACAGGTAGTT    61 CTGGTCCATT GGTGAAAGTT TGCGGCTTGC AGAGCACAGA GGCCGCAGAA TGTGCTCTAG   121 ATTCCGATGC TGACTTGCTG GGTATTATAT GTGTGCCCAA TAGAAAGAGA ACAATTGACC   181 CGGTTATTGC AAGGAAAATT TCAAGTCTTG TAAAAGCATA TAAAAATAGT TCAGGCACTC   241 CGAAATACTT GGTTGGCGTG TTTCGTAATC AACCTAAGGA GGATGTTTTG GCTCTGGTCA   301 ATGATTACGG CATTGATATC GTCCAACTGC ATGGAGATGA GTCGTGGCAA GAATACCAAG   361 AGTTCCTCGG TTTGCCAGTT ATTAAAAGAC TCGTATTTCC AAAAGACTGC AACATACTAC   421 TCAGTGCAGC TTCACAGAAA CCTCATTCGT TTATTCCCTT GTTTGATTCA GAAGCAGGTG   481 GGACAGGTGA ACTTTTGGAT TGGAACTCGA TTTCTGACTG GGTTGGAAGG CAAGAGAGCC   541 CCGAAAGCTT ACATTTTATG TTAGCTGGTG GACTGACGCC AGAAAATGTT GGTGATGCGC   601 TTAGATTAAA TGGCGTTATT GGTGTTGATG TAAGCGGAGG TGTGGAGACA AATGGTGTAA   661 AAGACTCTAA CAAAATAGCA AATTTCGTCA AAAATGCTAA GAAATAGGTT ATTACTGAGT   721 AGTATTTATT TAAGTATTGT TTGTGCACTT GCCGGTAGAG GTGTGGTCAA TAAGAGCGAC   781 CTCATGCTAT ACCTGAGAAA GCAACCTGAC CTACAGGAAA GAGTTACTCA AGAATAAGAA   841 TTTTCGTTTT AAAACCTAAG AGTCACTTTA AAATTTGTAT ACACTTATTT TTTTTTATAA   901 CTTATTTAAT AATAAAAATC ATAAATCATA AGAAATTCGC CCGGAATTAG CTTGGCTGCA   961 GGTCGACTCA CAGATCCTCT TCGGAAATCA GTTTCTGTTC AGAATTCGAG CTCGGTACCC  1021 GGGGGATCCC TCTAGAGTCA ATTTCGATCC GGGGGTTTTT TTTCTCCTTT GACGGTTAAG  1081 TATAGAGGTA TTATTTAACA CTTTTTTGTG ATACCTTTTA ATTTACATTT TGATAGAAGT 1141 TAAATACCAG ACCCGGAA //

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

2023.08.17

GNP_filter3_RDBDEP_html_230702.pl