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pGMT10

E. coli - S. cerevisiae shuttle vector

Catalog number RDB01959
Resource name pGMT10
Alternative name E. coli-S. cerevisiae shuttle vector
Clone info. E. coli-S. cerevisiae shuttle vector, 9E10 epitope (Myc-tag), centromere-type ori, TRP1, GAL1-10 promoter, Ampicillin
Vector backbone pGML20 (Plasmid)
Size of vector backbone 4,641 bp
Selectable markers Amp^r (E. coli), TRP1 (S. cerevisiae)
Depositor Iha, Hidekatsu |

Distribution information

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Terms and conditions set forth by the DEPOSITOR Acknowledgement to Depositor and Developer in publication.
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RDB01959 pGMT10 DNA solution Please contact us.

Ordering Information [in Japanese] [in English]

References and tips

Electronic file

Electronic file Sequence RDB01959z.seq from Depositor
Electronic file Map RDB01959.gif from Depositor
Electronic file Map RDB01959z.gif from Depositor

Featured content

Featured content Expression Vector Backbone of Saccharomyces cerevisiae (English text)

References

original Iha, H., Shuttle-vector system for Saccharomyces cerevisiae designed to produce C-terminal-Myc-tagged fusion proteins. Biotechniques, 25, 936-938 (1998). PMID 9863043.

User reports and related articles (go to bottom)


Sequence information

RIKEN BRC has sequenced portions of this material for quality test. Primers and the results are shown below.

Data are summerized on test sheet below.

Test sheet RDB13046_A4Lj.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: ADHrev RDB13046_A4Lja.seq
>01959_13046_A4Lj_ADHrev_C10_07.ab1
    1 TCAGATCTCA AGATAAGAAT TTTCGTTTTA AAACCTAAGA GTCACTTTAA AATTTGTATA
   61 CACTTATTTT TTTTATAACT TATTTAATAA TAAAAATCAT AAATCATAAG AAATTCGCCC
  121 GGAATTAGCT TGGCTGCAGG TCGACTCACA GATCCTCTTC GGAAATCAGT TTCTGTTCAG
  181 AATTCGAGCT CGGTACCCGG GGATCCTCTA GAGTCAATTC GATCCGGGGT TTTTTCTCCT
  241 TGACGTTAAA GTATAGAGGT ATATTAACAA TTTTTTGTTG ATACTTTTAT TACATTTGAA
  301 TAAGAAGTAA TACAAACCGA AAATGTTGAA AGTATTAGTT AAAGTGGTTA TGCAGTTTTT
  361 GCATTTATAT ATCTGTTAAT AGATCAAAAA TCATCGCTTC GCTGATTAAT TACCCCAGAA
  421 ATAAGGCTAA AAAACTAATC GCATTATCAT CCTATGGTTG TTAATTTGAT TCGTTCATTT
  481 GAAGGTTTGT GGGGCCAGGT TACTGCCAAT TTTTCCTCTT CATAACCATA AAAGCTAGTA
  541 TTGTAGAATC TTTATTGTTC GGAGCAGTGC GGCGCGAGGC ACATCTGCGT TTCAGGAACG
  601 CGACCGGTGA AGACGAGGAC GCACGGAGGA GAGTCTTCCT TCGGAGGGCT GTCACCCGCT
  661 CGGCGGCTTC TAATCCGTAC TTCAATATAG CAATGAGCAG TTAAGCGTAT TACTGAAAGT
  721 TCCAAAGAGA AGGTTTTTTT AGGCTAAGAT AATGGGGCTC TTTACATTTC CACAACATAT
  781 AAGTAAGATT AGATATGGAT ATGTATATGG ATATGTATAT GGTGGTAATG CCATGTAATA
  841 TGATTATTAA ACTTCTTTGC GTCCATCCAA AAAAAAAGTA AGAATTTTTT GAAAATTCAA
  901 TATAAATGAC AGCTCAGTTA CAAAGTGAAA GTGACCTGCA GGCATGCAAG CTCTGCATGT
  961 GTCAGAGGTT TTCACCGTCA TCACCGAAAC GCGCGAGACG AAAGGGCCTC GTGATACGCC
 1021 TATTTTTTTA TAGGTTAATG TCATGATAAT AATGGATTTT CTTACGGTCG TTTTTCAATC
 1081 ACGTGCTAAT GAAATAATAT AGTTGAAGCT TTTTTAATAT AACTATTATA AGTTTAAAAA
1141 AA
//
Primer: Amp_R RDB13046_A4Ljb.seq
>01959_13046_A4Lj_Amp_R_D10_10.ab1
    1 ACTTTTTTCA TATTTATTGA GCATTTATCA GGGTTATTGT CTCATGCAGC GGATACATAT
   61 TTGAATGTAT TTAGAAAAAT AAACAAATAG GGGTTCCGCG CACATTTCCC CGAAAAGTGC
  121 CACCTGACGG ATCGCTTGCC TGTAACTTAC ACGCGCCTCG TATCTTTTAA TGATGGAATA
  181 ATTTGGGAAT TTACTCTGTG TTTATTTATT TTTATGTTTT GTATTTGGAT TTTAGAAAGT
  241 AAATAAAGAA GGTAGAAGAG TTACGGAATG AAGAAAAAAA AATAAACAAA GGTTTAAAAA
  301 ATTTCAACAA AAAGCGTACT TTACATATAT ATTTATTAGA CAAGAAAAGC AGATTAAATA
  361 GATATACATT CGATTAACGA TAAGTAAAAT GTAAAATCAC AGGATTTTCG TGTGTGGTCT
  421 TCTACACAGA CAAGATGAAA CAATTCGGCA TTAATACCTG AGAGCAGGAA GAGCAAGATA
  481 AAAGGTAGTA TTTGTTGGCG ATCCCCCTAG AGTCTTTTAC ATCTTCGGAA AACAAAAACT
  541 ATTTTTTCTT TAATTTCTTT TTTTACTTTC TATTTTTAAT TTATATATTT ATATTAAAAA
  601 ATTTAAATTA TAATTATTTT TATAGCACGT GATGAAAAGG ACCCTAAGAA ACCATTATTA
  661 TCATGACATT AACCTATAAA AATAGGCGTA TCACGAGGCC CTTTCGTCTC GCGCGTTTCG
  721 GTGATGACGG TGAAAACCTC TGACACATGC AGAGCTTGCA TGCCTGCAGG TCACTTTCAC
  781 TTTGTAACTG AGCTGTCATT TATATTGAAT TTTCAAAAAT TCTTACTTTT TTTTTGGATG
  841 GACGCAAAGA AGTTTAATAA TCATATTACA TGGCATTACC ACCATATACA TATCCATATA
  901 CATATCCATA TCTAATCTTA CTTATATGTT GTGGAAATGT AAAGAGCCCC ATTATCTTAG
  961 CCTAAAAAAA ACCTTCTCTT TGGAAACTTT CAGTAATACG CTTAAACTGC TCATTGCTAT
 1021 ATTTGAAAGT ACGGATTAGA AGCCCGCCCG AGCGGGTGAC AGCCCCTCCG AAGGAGACTC
 1081 TCCTCCGTGC GTCCTCGTCC TTCAACGGAT CGCGATTCCT GAAAACGGCC AGAAATTGTT
1141 TGCCCTTTTC GTCAGC
//
Primer: TRP1pro_F RDB13046_A4Ljc.seq
>01959_13046_A4Lj_TRP1pro_F_E10_13.ab1
    1 GCTAAGCTAT AGCCACAAAG GCAGCTTGGA GTATGTCTGT TATTAATTTC ACAGGTAGTT
   61 CTGGTCCATT GGTGAAAGTT TGCGGCTTGC AGAGCACAGA GGCCGCAGAA TGTGCTCTAG
  121 ATTCCGATGC TGACTTGCTG GGTATTATAT GTGTGCCCAA TAGAAAGAGA ACAATTGACC
  181 CGGTTATTGC AAGGAAAATT TCAAGTCTTG TAAAAGCATA TAAAAATAGT TCAGGCACTC
  241 CGAAATACTT GGTTGGCGTG TTTCGTAATC AACCTAAGGA GGATGTTTTG GCTCTGGTCA
  301 ATGATTACGG CATTGATATC GTCCAACTGC ATGGAGATGA GTCGTGGCAA GAATACCAAG
  361 AGTTCCTCGG TTTGCCAGTT ATTAAAAGAC TCGTATTTCC AAAAGACTGC AACATACTAC
  421 TCAGTGCAGC TTCACAGAAA CCTCATTCGT TTATTCCCTT GTTTGATTCA GAAGCAGGTG
  481 GGACAGGTGA ACTTTTGGAT TGGAACTCGA TTTCTGACTG GGTTGGAAGG CAAGAGAGCC
  541 CCGAAAGCTT ACATTTTATG TTAGCTGGTG GACTGACGCC AGAAAATGTT GGTGATGCGC
  601 TTAGATTAAA TGGCGTTATT GGTGTTGATG TAAGCGGAGG TGTGGAGACA AATGGTGTAA
  661 AAGACTCTAA CAAAATAGCA AATTTCGTCA AAAATGCTAA GAAATAGGTT ATTACTGAGT
  721 AGTATTTATT TAAGTATTGT TTGTGCACTT GCCGGTAGAG GTGTGGTCAA TAAGAGCGAC
  781 CTCATGCTAT ACCTGAGAAA GCAACCTGAC CTACAGGAAA GAGTTACTCA AGAATAAGAA
  841 TTTTCGTTTT AAAACCTAAG AGTCACTTTA AAATTTGTAT ACACTTATTT TTTTTTATAA
  901 CTTATTTAAT AATAAAAATC ATAAATCATA AGAAATTCGC CCGGAATTAG CTTGGCTGCA
  961 GGTCGACTCA CAGATCCTCT TCGGAAATCA GTTTCTGTTC AGAATTCGAG CTCGGTACCC
 1021 GGGGGATCCC TCTAGAGTCA ATTTCGATCC GGGGGTTTTT TTTCTCCTTT GACGGTTAAG
 1081 TATAGAGGTA TTATTTAACA CTTTTTTGTG ATACCTTTTA ATTTACATTT TGATAGAAGT
1141 TAAATACCAG ACCCGGAA
//

Please visit Sequencing and PCR primers for primer information.


References

References

original Iha, H., Shuttle-vector system for Saccharomyces cerevisiae designed to produce C-terminal-Myc-tagged fusion proteins. Biotechniques, 25, 936-938 (1998). PMID 9863043.

2018.12.10

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