Resource data sheet
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Please review the QC test results indicated by check icon below as well as clone information before placing your order.

pGML20 (#RDB01958)

E. coli - S. cerevisiae shuttle vector

Alternative name E. coli-S. cerevisiae shuttle vector
Clone info. E. coli-S. cerevisiae shuttle vector, 9E10 epitope (Myc-tag), 2micron ori, LEU2, GAL1-10 promoter, Ampicillin
Vector backbone pGML20 (Plasmid)
Size of vector backbone 6,238 bp
Selectable markers Amp^r (E. coli), LEU2 (S. cerevisiae)
Depositor|Developer Iha, Hidekatsu |
 
Sequence RDB01958z.seq
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RDB01958 pGML20 DNA solution Please contact us.

Please review the QC test results indicated by check icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The pGML20 was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB01958).

Reference section:

Iha, H., Tsurugi, K., Shuttle-vector system for Saccharomyces cerevisiae designed to produce C-terminal-Myc-tagged fusion proteins. Biotechniques, 25, 936-938 (1998). PMID 9863043. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB12315_A5Bg.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: ADH_rev
Sequence file: RDB12315_A5Bga.seq check
>01958_12315_A5Bg_ADHrev_A08_02.ab1
    1 TGGGGTTCTC AAGATAAGAT TTTCGTTTTA AACCTAAGAG TCACCCCTAA AATTTGTATA
   61 CACTTATTTT TTTTATAACT TATTTAATAA TAAAAATCAT AAATCATAAG AAATTCGCCC
  121 GGAATTAGCT TGGCTGCAGG TCGACTCACA GATCCTCTTC GGAAATCAGT TTCTGTTCAG
  181 AATTCGAGCT CGGTACCCGG GGATCCTCTA GAGTCAATTC GATCCGGGGT TTTTTCTCCT
  241 TGACGTTAAA GTATAGAGGT ATATTAACAA TTTTTTGTTG ATACTTTTAT TACATTTGAA
  301 TAAGAAGTAA TACAAACCGA AAATGTTGAA AGTATTAGTT AAAGTGGTTA TGCAGTTTTT
  361 GCATTTATAT ATCTGTTAAT AGATCAAAAA TCATCGCTTC GCTGATTAAT TACCCCAGAA
  421 ATAAGGCTAA AAAACTAATC GCATTATCAT CCTATGGTTG TTAATTTGAT TCGTTCATTT
  481 GAAGGTTTGT GGGGCCAGGT TACTGCCAAT TTTTCCTCTT CATAACCATA AAAGCTAGTA
  541 TTGTAGAATC TTTATTGTTC GGAGCAGTGC GGCGCGAGGC ACATCTGCGT TTCAGGAACG
  601 CGACCGGTGA AGACGAGGAC GCACGGAGGA GAGTCTTCCT TCGGAGGGCT GTCACCCGCT
  661 CGGCGGCTTC TAATCCGTAC TTCAATATAG CAATGAGCAG TTAAGCGTAT TACTGAAAGT
  721 TCCAAAGAGA AGGTTTTTTT AGGCTAAGAT AATGGGGCTC TTTACATTTC CACAACATAT
  781 AAGTAAGATT AGATATGGAT ATGTATATGG ATATGTATAT GGTGGTAATG CCATGTAATA
  841 TGATTATTAA ACTTCTTTGC GTCCATCCAA AAAAAAAGTA AGAATTTTTT GAAAATTCAA
  901 TATAAATGAC AGCTCAGTTA CAAAGTGAAA GTGACCTGCA GGCATGCAAG CAACGAAGCA
  961 TCTGTGCTTC ATTTTGTAGA ACAAAAATGC AACGCGAGAG CGCTAATTTT TTCAAACAAG
 1021 AAATCTGAGC TGCATTTTTT ACAGACAGAA ATGCAACGCG AAAGCGCTAT TTTTACGACG
1081 AAAGAATCTG TGCTCAATTT TTGGTAAACC AAAAATTGCA ACGTCGAAGA AGTCGTCT
//
Primer: Amp_R
Sequence file: RDB12315_A5Bgb.seq check
>01958_12315_A5Bg_Amp_R_B08_05.ab1
    1 TCAGTTTTCA TATTATTGAG CATTTATCAG GGTTATTGTC TCATGAGCGG ATACATATTT
   61 GAATGTATTT AGAAAAATAA ACAAATAGGG GTTCCGCGCA CATTTCCCCG AAAAGTGCCA
  121 CCTGACGTCT AAGAAACCAT TATTATCATG ACATTAACCT ATAAAAATAG GCGTATCACG
  181 AGGCCCTTTC GTCTCGCGCG TTTCGGTGAT GACGGTGAAA ACCTCTGACA CATGCAGCTC
  241 CCGGAGACGG TCACAGCTTG TCTGTAAGCG GATGCCGGGA GCAGACAAGC CCGTCAGGGC
  301 GCGTCAGCGG GTGTTGGCGG GTGTCGGGGC TGGCTTAACT ATGCGGCATC AGAGCAGATT
  361 GTACTGAGAG TGCACCATAA CGCATTTAAG CATAAACACG CACTATGCCG TTCTTCTCAT
  421 GTATATATAT ATACAGGCAA CACGCAGATA TAGGTGCGAC GTGAACAGTG AGCTGTATGT
  481 GCGCAGCTCG CGTTGCATTT TCGGAAGCGC TCGTTTTCGG AAACGCTTTG AAGTTCCTAT
  541 TCCGAAGTTC CTATTCTCTA GCTAGAAAGT ATAGGAACTT CAGAGCGCTT TTGAAAACCA
  601 AAAGCGCTCT GAAGACGCAC TTTCAAAAAA CCAAAAACGC ACCGGACTGT AACGAGCTAC
  661 TAAAATATTG CGAATACCGC TTCCACAAAC ATTGCTCAAA AGTATCTCTT TGCTATATAT
  721 CTCTGTGCTA TATCCCTATA TAACCTACCC ATCCACCTTT CGCTCCTTGA ACTTGCATCT
  781 AAACTCGACC TCTACATTTT TTATGTTTAT CTCTAGTATT ACTCTTTAGA CAAAAAAAAT
  841 TGTAGTAAGA ACTATTCATA GAGTGAATCG AAAACAATAC GAAAATGTAA ACATTTCCTA
  901 TACGTAGTAT ATAGAGACAA AATAGAAGAA ACCGTTCATA ATTTTCTGAC CAATGAAAGA
  961 ATCATCAACG CTATCACTTT CTGTTCACAA AGTATGCGCA AATCCCACAT CGGTATAGAA
 1021 TATAAATCGG GGATGCCCTT TATCTGAAAA AAATGCACCC GCAGCCTTCG CTAGTATCAG
 1081 TAAACGCGGA AGTGCAGTTC AAGCTTTTTT TATGGCAAGA GCAAAATAGG AACCACCGAA
1141 AAGGTTAG
//
Primer: LEU2pro_F
Sequence file: RDB12315_A5Bgc.seq check
>01958_12315_A5Bg_LEU2pro_F_G11_20.ab1
    1 ATAGAGCTGC TATTATTTAA GGACCTATTG TTTTTTCCAA TAGGTGGTTA GCAATCGTCT
   61 TACTTTCTAA CTTTTCTTAC CTTTTACATT TCAGCAATAT ATATATATAT ATTTCAAGGA
  121 TATACCATTC TAATGTCTGC CCCTAAGAAG ATCGTCGTTT TGCCAGGTGA CCACGTTGGT
  181 CAAGAAATCA CAGCCGAAGC CATTAAGGTT CTTAAAGCTA TTTCTGATGT TCGTTCCAAT
  241 GTCAAGTTCG ATTTCGAAAA TCATTTAATT GGTGGTGCTG CTATCGATGC TACAGGTGTT
  301 CCACTTCCAG ATGAGGCGCT GGAAGCCTCC AAGAAGGCTG ATGCCGTTTT GTTAGGTGCT
  361 GTGGGTGGTC CTAAATGGGG TACAGGTAGT GTTAGACCTG AACAAGGTTT ACTAAAAATC
  421 CGTAAAGAAC TTCAATTGTA CGCCAACTTA AGACCATGTA ACTTTGCATC CGACTCTCTT
  481 TTAGACTTAT CTCCAATCAA GCCACAATTT GCTAAAGGTA CTGACTTCGT TGTTGTCAGA
  541 GAATTAGTGG GAGGTATTTA CTTTGGTAAG AGAAAGGAAG ACGATGGTGA TGGTGTCGCT
  601 TGGGATAGTG AACAATACAC CGTTCCAGAA GTGCAAAGAA TCACAAGAAT GGCCGCTTTC
  661 ATGGCCCTAC AACATGAGCC ACCATTGCCT ATTTGGTCCT TGGATAAAGC TAATGTTTTG
  721 GCCTCTTCAA GATTATGGAG AAAACTGTGG AGGAAACCAT CAAGAACGAA TTTCCTACAT
  781 TGAAGGTTCA ACATCAATTG ATTGATTCTG CCGCCATGAT CCTAGTTAAG AACCCAACCC
  841 ACCTAAATGG TATTATAATC ACCAGCAACA TGTTTGGTGA TATCATCTCC GATGAAGCCT
  901 CCGTTATCCC AGGTTCCTTG GGTTTGTTGC CATCTGCGTC CTTGGCCTCT TTGCCAGACA
  961 AGAACACCGC ATTTGGGTTT GTACGAACCC ATGCCACGGG TTCTGCTCCA GATTTGCCAA
 1021 AGAATAACGT CCACCCTATC GCACTATCTG TCTGCTTGCC ATTGATGTGG AAAGTGGTCA
1081 TTTGACTTGC TGAGAGGTAC GTCATTGGAG ATGCAGTTTA AAAAAAGGCG T
//

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

original Iha, H., Shuttle-vector system for Saccharomyces cerevisiae designed to produce C-terminal-Myc-tagged fusion proteins. Biotechniques, 25, 936-938 (1998). PMID 9863043. [link to RRC of NBRP]
user_report Shigematsu, M., Evidence for DNA cleavage caused directly by a transfer RNA-targeting toxin. PLoS One. 8 (9): e75512 (2013). PMID 24069426. [link to RRC of NBRP]