Resource data sheet
Prev.
Please review the QC test results indicated by check icon below as well as clone information before placing your order.

pGML20

E. coli - S. cerevisiae shuttle vector

Catalog number RDB01958
Resource name pGML20
Alternative name E. coli-S. cerevisiae shuttle vector
Clone info. E. coli-S. cerevisiae shuttle vector, 9E10 epitope (Myc-tag), 2micron ori, LEU2, GAL1-10 promoter, Ampicillin
Vector backbone pGML20 (Plasmid)
Size of vector backbone 6,238 bp
Selectable markers Amp^r (E. coli), LEU2 (S. cerevisiae)
Depositor|Developer Iha, Hidekatsu |
Other clones in our bank

External Database

            Reference sequence
              

            Distribution information

            Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
            Terms and conditions set forth by the DEPOSITOR Acknowledgement to Depositor and Developer in publication.
            Ordering Please visit Information of Request for Distribution.[link] 
            Order form 
            [Credit Card Payment]  [Bank Transfer Payment]
            Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word]
            提供案内 (日本国内) [open/close]

            提供条件 研究結果の発表時、謝辞に寄託者および樹立者名を入れること。
            提供依頼 手続きの詳細は、「提供申込みについて[link]」をご覧ください。
            提供依頼書 [Word]
            提供同意書 (MTA、非営利機関による非営利学術研究用)[Word]

            Catalog # Resource name Shipping form Fee (non-profit org.)
            RDB01958 pGML20 DNA solution Please contact us.

            Ordering Information [in Japanese] [in English]
            Please review the QC test results indicated by check icon below as well as clone information before placing your order.

            References and tips

            Electronic file

            Sequence RDB01958z.seq
            Remarks, protocol and/or map (gif) RDB01958.gif
            Remarks, protocol and/or map (gif) RDB01958z.gif

            Original reference

            original Iha, H., Shuttle-vector system for Saccharomyces cerevisiae designed to produce C-terminal-Myc-tagged fusion proteins. Biotechniques, 25, 936-938 (1998). PMID 9863043.

            Further references such as user reports and related articles (go to bottom)

            Featured content

            Featured content Expression Vector Backbone of Saccharomyces cerevisiae (English text)

            Sequence information

            RIKEN BRC has sequenced portions of this material for quality test.
            Please review the QC test results indicated by check icon as well as clone information before placing your order.

            Test sheet RDB12315_A5Bg.pdf check

            Nucleotide sequence of a portion of this resource (if available).

            Primer: ADH_rev
            Sequence file: RDB12315_A5Bga.seq check
            >01958_12315_A5Bg_ADHrev_A08_02.ab1
                1 TGGGGTTCTC AAGATAAGAT TTTCGTTTTA AACCTAAGAG TCACCCCTAA AATTTGTATA
               61 CACTTATTTT TTTTATAACT TATTTAATAA TAAAAATCAT AAATCATAAG AAATTCGCCC
              121 GGAATTAGCT TGGCTGCAGG TCGACTCACA GATCCTCTTC GGAAATCAGT TTCTGTTCAG
              181 AATTCGAGCT CGGTACCCGG GGATCCTCTA GAGTCAATTC GATCCGGGGT TTTTTCTCCT
              241 TGACGTTAAA GTATAGAGGT ATATTAACAA TTTTTTGTTG ATACTTTTAT TACATTTGAA
              301 TAAGAAGTAA TACAAACCGA AAATGTTGAA AGTATTAGTT AAAGTGGTTA TGCAGTTTTT
              361 GCATTTATAT ATCTGTTAAT AGATCAAAAA TCATCGCTTC GCTGATTAAT TACCCCAGAA
              421 ATAAGGCTAA AAAACTAATC GCATTATCAT CCTATGGTTG TTAATTTGAT TCGTTCATTT
              481 GAAGGTTTGT GGGGCCAGGT TACTGCCAAT TTTTCCTCTT CATAACCATA AAAGCTAGTA
              541 TTGTAGAATC TTTATTGTTC GGAGCAGTGC GGCGCGAGGC ACATCTGCGT TTCAGGAACG
              601 CGACCGGTGA AGACGAGGAC GCACGGAGGA GAGTCTTCCT TCGGAGGGCT GTCACCCGCT
              661 CGGCGGCTTC TAATCCGTAC TTCAATATAG CAATGAGCAG TTAAGCGTAT TACTGAAAGT
              721 TCCAAAGAGA AGGTTTTTTT AGGCTAAGAT AATGGGGCTC TTTACATTTC CACAACATAT
              781 AAGTAAGATT AGATATGGAT ATGTATATGG ATATGTATAT GGTGGTAATG CCATGTAATA
              841 TGATTATTAA ACTTCTTTGC GTCCATCCAA AAAAAAAGTA AGAATTTTTT GAAAATTCAA
              901 TATAAATGAC AGCTCAGTTA CAAAGTGAAA GTGACCTGCA GGCATGCAAG CAACGAAGCA
              961 TCTGTGCTTC ATTTTGTAGA ACAAAAATGC AACGCGAGAG CGCTAATTTT TTCAAACAAG
             1021 AAATCTGAGC TGCATTTTTT ACAGACAGAA ATGCAACGCG AAAGCGCTAT TTTTACGACG
            1081 AAAGAATCTG TGCTCAATTT TTGGTAAACC AAAAATTGCA ACGTCGAAGA AGTCGTCT
            //
            Primer: Amp_R
            Sequence file: RDB12315_A5Bgb.seq check
            >01958_12315_A5Bg_Amp_R_B08_05.ab1
                1 TCAGTTTTCA TATTATTGAG CATTTATCAG GGTTATTGTC TCATGAGCGG ATACATATTT
               61 GAATGTATTT AGAAAAATAA ACAAATAGGG GTTCCGCGCA CATTTCCCCG AAAAGTGCCA
              121 CCTGACGTCT AAGAAACCAT TATTATCATG ACATTAACCT ATAAAAATAG GCGTATCACG
              181 AGGCCCTTTC GTCTCGCGCG TTTCGGTGAT GACGGTGAAA ACCTCTGACA CATGCAGCTC
              241 CCGGAGACGG TCACAGCTTG TCTGTAAGCG GATGCCGGGA GCAGACAAGC CCGTCAGGGC
              301 GCGTCAGCGG GTGTTGGCGG GTGTCGGGGC TGGCTTAACT ATGCGGCATC AGAGCAGATT
              361 GTACTGAGAG TGCACCATAA CGCATTTAAG CATAAACACG CACTATGCCG TTCTTCTCAT
              421 GTATATATAT ATACAGGCAA CACGCAGATA TAGGTGCGAC GTGAACAGTG AGCTGTATGT
              481 GCGCAGCTCG CGTTGCATTT TCGGAAGCGC TCGTTTTCGG AAACGCTTTG AAGTTCCTAT
              541 TCCGAAGTTC CTATTCTCTA GCTAGAAAGT ATAGGAACTT CAGAGCGCTT TTGAAAACCA
              601 AAAGCGCTCT GAAGACGCAC TTTCAAAAAA CCAAAAACGC ACCGGACTGT AACGAGCTAC
              661 TAAAATATTG CGAATACCGC TTCCACAAAC ATTGCTCAAA AGTATCTCTT TGCTATATAT
              721 CTCTGTGCTA TATCCCTATA TAACCTACCC ATCCACCTTT CGCTCCTTGA ACTTGCATCT
              781 AAACTCGACC TCTACATTTT TTATGTTTAT CTCTAGTATT ACTCTTTAGA CAAAAAAAAT
              841 TGTAGTAAGA ACTATTCATA GAGTGAATCG AAAACAATAC GAAAATGTAA ACATTTCCTA
              901 TACGTAGTAT ATAGAGACAA AATAGAAGAA ACCGTTCATA ATTTTCTGAC CAATGAAAGA
              961 ATCATCAACG CTATCACTTT CTGTTCACAA AGTATGCGCA AATCCCACAT CGGTATAGAA
             1021 TATAAATCGG GGATGCCCTT TATCTGAAAA AAATGCACCC GCAGCCTTCG CTAGTATCAG
             1081 TAAACGCGGA AGTGCAGTTC AAGCTTTTTT TATGGCAAGA GCAAAATAGG AACCACCGAA
            1141 AAGGTTAG
            //
            Primer: LEU2pro_F
            Sequence file: RDB12315_A5Bgc.seq check
            >01958_12315_A5Bg_LEU2pro_F_G11_20.ab1
                1 ATAGAGCTGC TATTATTTAA GGACCTATTG TTTTTTCCAA TAGGTGGTTA GCAATCGTCT
               61 TACTTTCTAA CTTTTCTTAC CTTTTACATT TCAGCAATAT ATATATATAT ATTTCAAGGA
              121 TATACCATTC TAATGTCTGC CCCTAAGAAG ATCGTCGTTT TGCCAGGTGA CCACGTTGGT
              181 CAAGAAATCA CAGCCGAAGC CATTAAGGTT CTTAAAGCTA TTTCTGATGT TCGTTCCAAT
              241 GTCAAGTTCG ATTTCGAAAA TCATTTAATT GGTGGTGCTG CTATCGATGC TACAGGTGTT
              301 CCACTTCCAG ATGAGGCGCT GGAAGCCTCC AAGAAGGCTG ATGCCGTTTT GTTAGGTGCT
              361 GTGGGTGGTC CTAAATGGGG TACAGGTAGT GTTAGACCTG AACAAGGTTT ACTAAAAATC
              421 CGTAAAGAAC TTCAATTGTA CGCCAACTTA AGACCATGTA ACTTTGCATC CGACTCTCTT
              481 TTAGACTTAT CTCCAATCAA GCCACAATTT GCTAAAGGTA CTGACTTCGT TGTTGTCAGA
              541 GAATTAGTGG GAGGTATTTA CTTTGGTAAG AGAAAGGAAG ACGATGGTGA TGGTGTCGCT
              601 TGGGATAGTG AACAATACAC CGTTCCAGAA GTGCAAAGAA TCACAAGAAT GGCCGCTTTC
              661 ATGGCCCTAC AACATGAGCC ACCATTGCCT ATTTGGTCCT TGGATAAAGC TAATGTTTTG
              721 GCCTCTTCAA GATTATGGAG AAAACTGTGG AGGAAACCAT CAAGAACGAA TTTCCTACAT
              781 TGAAGGTTCA ACATCAATTG ATTGATTCTG CCGCCATGAT CCTAGTTAAG AACCCAACCC
              841 ACCTAAATGG TATTATAATC ACCAGCAACA TGTTTGGTGA TATCATCTCC GATGAAGCCT
              901 CCGTTATCCC AGGTTCCTTG GGTTTGTTGC CATCTGCGTC CTTGGCCTCT TTGCCAGACA
              961 AGAACACCGC ATTTGGGTTT GTACGAACCC ATGCCACGGG TTCTGCTCCA GATTTGCCAA
             1021 AGAATAACGT CCACCCTATC GCACTATCTG TCTGCTTGCC ATTGATGTGG AAAGTGGTCA
            1081 TTTGACTTGC TGAGAGGTAC GTCATTGGAG ATGCAGTTTA AAAAAAGGCG T
            //

            Please visit Sequencing and PCR primers for primer information.


            References

            Original, user report and related articles

            original Iha, H., Shuttle-vector system for Saccharomyces cerevisiae designed to produce C-terminal-Myc-tagged fusion proteins. Biotechniques, 25, 936-938 (1998). PMID 9863043.
            user report Shigematsu, M., Evidence for DNA cleavage caused directly by a transfer RNA-targeting toxin. PLoS One. 8 (9): e75512 (2013). PMID 24069426.

            2022.02.09

            GNP_filter3_RDBDEP_html_220112.pl