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pGML20

E. coli - S. cerevisiae shuttle vector

Catalog number RDB01958
Resource name pGML20
Alternative name E. coli-S. cerevisiae shuttle vector
Clone info. E. coli-S. cerevisiae shuttle vector, 9E10 epitope (Myc-tag), 2micron ori, LEU2, GAL1-10 promoter, Ampicillin
Vector backbone pGML20 (Plasmid)
Size of vector backbone 6,238 bp
Selectable markers Amp^r (E. coli), LEU2 (S. cerevisiae)
Depositor Iha, Hidekatsu |

Distribution information

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Terms and conditions set forth by the DEPOSITOR Acknowledgement to Depositor and Developer in publication.
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RDB01958 pGML20 DNA solution Please contact us.

Ordering Information [in Japanese] [in English]

References and tips

Electronic file

Electronic file Sequence RDB01958z.seq from Depositor
Electronic file Map RDB01958.gif from Depositor
Electronic file Map RDB01958z.gif from Depositor

Featured content

Featured content Expression Vector Backbone of Saccharomyces cerevisiae (English text)

References

original Iha, H., Shuttle-vector system for Saccharomyces cerevisiae designed to produce C-terminal-Myc-tagged fusion proteins. Biotechniques, 25, 936-938 (1998). PMID 9863043.

User reports and related articles (go to bottom)


Sequence information

RIKEN BRC has sequenced portions of this material for quality test. Primers and the results are shown below.

Data are summerized on test sheet below.

Test sheet RDB12315_A5Bg.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: ADH_rev RDB12315_A5Bga.seq
>01958_12315_A5Bg_ADHrev_A08_02.ab1
    1 TGGGGTTCTC AAGATAAGAT TTTCGTTTTA AACCTAAGAG TCACCCCTAA AATTTGTATA
   61 CACTTATTTT TTTTATAACT TATTTAATAA TAAAAATCAT AAATCATAAG AAATTCGCCC
  121 GGAATTAGCT TGGCTGCAGG TCGACTCACA GATCCTCTTC GGAAATCAGT TTCTGTTCAG
  181 AATTCGAGCT CGGTACCCGG GGATCCTCTA GAGTCAATTC GATCCGGGGT TTTTTCTCCT
  241 TGACGTTAAA GTATAGAGGT ATATTAACAA TTTTTTGTTG ATACTTTTAT TACATTTGAA
  301 TAAGAAGTAA TACAAACCGA AAATGTTGAA AGTATTAGTT AAAGTGGTTA TGCAGTTTTT
  361 GCATTTATAT ATCTGTTAAT AGATCAAAAA TCATCGCTTC GCTGATTAAT TACCCCAGAA
  421 ATAAGGCTAA AAAACTAATC GCATTATCAT CCTATGGTTG TTAATTTGAT TCGTTCATTT
  481 GAAGGTTTGT GGGGCCAGGT TACTGCCAAT TTTTCCTCTT CATAACCATA AAAGCTAGTA
  541 TTGTAGAATC TTTATTGTTC GGAGCAGTGC GGCGCGAGGC ACATCTGCGT TTCAGGAACG
  601 CGACCGGTGA AGACGAGGAC GCACGGAGGA GAGTCTTCCT TCGGAGGGCT GTCACCCGCT
  661 CGGCGGCTTC TAATCCGTAC TTCAATATAG CAATGAGCAG TTAAGCGTAT TACTGAAAGT
  721 TCCAAAGAGA AGGTTTTTTT AGGCTAAGAT AATGGGGCTC TTTACATTTC CACAACATAT
  781 AAGTAAGATT AGATATGGAT ATGTATATGG ATATGTATAT GGTGGTAATG CCATGTAATA
  841 TGATTATTAA ACTTCTTTGC GTCCATCCAA AAAAAAAGTA AGAATTTTTT GAAAATTCAA
  901 TATAAATGAC AGCTCAGTTA CAAAGTGAAA GTGACCTGCA GGCATGCAAG CAACGAAGCA
  961 TCTGTGCTTC ATTTTGTAGA ACAAAAATGC AACGCGAGAG CGCTAATTTT TTCAAACAAG
 1021 AAATCTGAGC TGCATTTTTT ACAGACAGAA ATGCAACGCG AAAGCGCTAT TTTTACGACG
1081 AAAGAATCTG TGCTCAATTT TTGGTAAACC AAAAATTGCA ACGTCGAAGA AGTCGTCT
//
Primer: Amp_R RDB12315_A5Bgb.seq
>01958_12315_A5Bg_Amp_R_B08_05.ab1
    1 TCAGTTTTCA TATTATTGAG CATTTATCAG GGTTATTGTC TCATGAGCGG ATACATATTT
   61 GAATGTATTT AGAAAAATAA ACAAATAGGG GTTCCGCGCA CATTTCCCCG AAAAGTGCCA
  121 CCTGACGTCT AAGAAACCAT TATTATCATG ACATTAACCT ATAAAAATAG GCGTATCACG
  181 AGGCCCTTTC GTCTCGCGCG TTTCGGTGAT GACGGTGAAA ACCTCTGACA CATGCAGCTC
  241 CCGGAGACGG TCACAGCTTG TCTGTAAGCG GATGCCGGGA GCAGACAAGC CCGTCAGGGC
  301 GCGTCAGCGG GTGTTGGCGG GTGTCGGGGC TGGCTTAACT ATGCGGCATC AGAGCAGATT
  361 GTACTGAGAG TGCACCATAA CGCATTTAAG CATAAACACG CACTATGCCG TTCTTCTCAT
  421 GTATATATAT ATACAGGCAA CACGCAGATA TAGGTGCGAC GTGAACAGTG AGCTGTATGT
  481 GCGCAGCTCG CGTTGCATTT TCGGAAGCGC TCGTTTTCGG AAACGCTTTG AAGTTCCTAT
  541 TCCGAAGTTC CTATTCTCTA GCTAGAAAGT ATAGGAACTT CAGAGCGCTT TTGAAAACCA
  601 AAAGCGCTCT GAAGACGCAC TTTCAAAAAA CCAAAAACGC ACCGGACTGT AACGAGCTAC
  661 TAAAATATTG CGAATACCGC TTCCACAAAC ATTGCTCAAA AGTATCTCTT TGCTATATAT
  721 CTCTGTGCTA TATCCCTATA TAACCTACCC ATCCACCTTT CGCTCCTTGA ACTTGCATCT
  781 AAACTCGACC TCTACATTTT TTATGTTTAT CTCTAGTATT ACTCTTTAGA CAAAAAAAAT
  841 TGTAGTAAGA ACTATTCATA GAGTGAATCG AAAACAATAC GAAAATGTAA ACATTTCCTA
  901 TACGTAGTAT ATAGAGACAA AATAGAAGAA ACCGTTCATA ATTTTCTGAC CAATGAAAGA
  961 ATCATCAACG CTATCACTTT CTGTTCACAA AGTATGCGCA AATCCCACAT CGGTATAGAA
 1021 TATAAATCGG GGATGCCCTT TATCTGAAAA AAATGCACCC GCAGCCTTCG CTAGTATCAG
 1081 TAAACGCGGA AGTGCAGTTC AAGCTTTTTT TATGGCAAGA GCAAAATAGG AACCACCGAA
1141 AAGGTTAG
//
Primer: LEU2pro_F RDB12315_A5Bgc.seq
>01958_12315_A5Bg_LEU2pro_F_G11_20.ab1
    1 ATAGAGCTGC TATTATTTAA GGACCTATTG TTTTTTCCAA TAGGTGGTTA GCAATCGTCT
   61 TACTTTCTAA CTTTTCTTAC CTTTTACATT TCAGCAATAT ATATATATAT ATTTCAAGGA
  121 TATACCATTC TAATGTCTGC CCCTAAGAAG ATCGTCGTTT TGCCAGGTGA CCACGTTGGT
  181 CAAGAAATCA CAGCCGAAGC CATTAAGGTT CTTAAAGCTA TTTCTGATGT TCGTTCCAAT
  241 GTCAAGTTCG ATTTCGAAAA TCATTTAATT GGTGGTGCTG CTATCGATGC TACAGGTGTT
  301 CCACTTCCAG ATGAGGCGCT GGAAGCCTCC AAGAAGGCTG ATGCCGTTTT GTTAGGTGCT
  361 GTGGGTGGTC CTAAATGGGG TACAGGTAGT GTTAGACCTG AACAAGGTTT ACTAAAAATC
  421 CGTAAAGAAC TTCAATTGTA CGCCAACTTA AGACCATGTA ACTTTGCATC CGACTCTCTT
  481 TTAGACTTAT CTCCAATCAA GCCACAATTT GCTAAAGGTA CTGACTTCGT TGTTGTCAGA
  541 GAATTAGTGG GAGGTATTTA CTTTGGTAAG AGAAAGGAAG ACGATGGTGA TGGTGTCGCT
  601 TGGGATAGTG AACAATACAC CGTTCCAGAA GTGCAAAGAA TCACAAGAAT GGCCGCTTTC
  661 ATGGCCCTAC AACATGAGCC ACCATTGCCT ATTTGGTCCT TGGATAAAGC TAATGTTTTG
  721 GCCTCTTCAA GATTATGGAG AAAACTGTGG AGGAAACCAT CAAGAACGAA TTTCCTACAT
  781 TGAAGGTTCA ACATCAATTG ATTGATTCTG CCGCCATGAT CCTAGTTAAG AACCCAACCC
  841 ACCTAAATGG TATTATAATC ACCAGCAACA TGTTTGGTGA TATCATCTCC GATGAAGCCT
  901 CCGTTATCCC AGGTTCCTTG GGTTTGTTGC CATCTGCGTC CTTGGCCTCT TTGCCAGACA
  961 AGAACACCGC ATTTGGGTTT GTACGAACCC ATGCCACGGG TTCTGCTCCA GATTTGCCAA
 1021 AGAATAACGT CCACCCTATC GCACTATCTG TCTGCTTGCC ATTGATGTGG AAAGTGGTCA
1081 TTTGACTTGC TGAGAGGTAC GTCATTGGAG ATGCAGTTTA AAAAAAGGCG T
//

Please visit Sequencing and PCR primers for primer information.


References

References

original Iha, H., Shuttle-vector system for Saccharomyces cerevisiae designed to produce C-terminal-Myc-tagged fusion proteins. Biotechniques, 25, 936-938 (1998). PMID 9863043.
user report Shigematsu, M., Evidence for DNA cleavage caused directly by a transfer RNA-targeting toxin. PLoS One. 8 (9): e75512 (2013). PMID 24069426.

2018.12.10

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