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Please review the QC test results indicated by check icon below as well as clone information before placing your order.

pGML10 (#RDB01957)

E. coli - S. cerevisiae shuttle vector

Alternative name E. coli-S. cerevisiae shuttle vector
Clone info. E. coli-S. cerevisiae shuttle vector, 9E10 epitope (Myc-tag), centromere-type ori, LEU2, GAL1-10 promoter, Ampicillin
Vector backbone pGML10 (Plasmid)
Size of vector backbone 5,446 bp
Selectable markers Amp^r (E. coli), LEU2 (S. cerevisiae)
Depositor|Developer Iha, Hidekatsu |
 
Sequence RDB01957z.seq
Additional map

Distribution information

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RDB01957 pGML10 DNA solution Please contact us.

Please review the QC test results indicated by check icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The pGML10 was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB01957).

Reference section:

Iha, H., Tsurugi, K., Shuttle-vector system for Saccharomyces cerevisiae designed to produce C-terminal-Myc-tagged fusion proteins. Biotechniques, 25, 936-938 (1998). PMID 9863043. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB12344_A3Lk.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: ADHrev
Sequence file: RDB12344_A3Lka.seq check
>01957_12344_A3Lk_ADHrev_A12_03.ab1
    1 TTAGGGATTA ATCAGGAATA AGATTTTCGT TTTAAACCTA AGAGTCACTT TAAAATTTGT
   61 ATACACTTAT TTTTTTTATA ACTTATTTAA TAATAAAAAT CATAAATCAT AAGAAATTCG
  121 CCCGGAATTA GCTTGGCTGC AGGTCGACTC ACAGATCCTC TTCGGAAATC AGTTTCTGTT
  181 CAGAATTCGA GCTCGGTACC CGGGGATCCT CTAGAGTCAA TTCGATCCGG GGTTTTTTCT
  241 CCTTGACGTT AAAGTATAGA GGTATATTAA CAATTTTTTG TTGATACTTT TATTACATTT
  301 GAATAAGAAG TAATACAAAC CGAAAATGTT GAAAGTATTA GTTAAAGTGG TTATGCAGTT
  361 TTTGCATTTA TATATCTGTT AATAGATCAA AAATCATCGC TTCGCTGATT AATTACCCCA
  421 GAAATAAGGC TAAAAAACTA ATCGCATTAT CATCCTATGG TTGTTAATTT GATTCGTTCA
  481 TTTGAAGGTT TGTGGGGCCA GGTTACTGCC AATTTTTCCT CTTCATAACC ATAAAAGCTA
  541 GTATTGTAGA ATCTTTATTG TTCGGAGCAG TGCGGCGCGA GGCACATCTG CGTTTCAGGA
  601 ACGCGACCGG TGAAGACGAG GACGCACGGA GGAGAGTCTT CCTTCGGAGG GCTGTCACCC
  661 GCTCGGCGGC TTCTAATCCG TACTTCAATA TAGCAATGAG CAGTTAAGCG TATTACTGAA
  721 AGTTCCAAAG AGAAGGTTTT TTTAGGCTAA GATAATGGGG CTCTTTACAT TTCCACAACA
  781 TATAAGTAAG ATTAGATATG GATATGTATA TGGATATGTA TATGGTGGTA ATGCCATGTA
  841 ATATGATTAT TAAACTTCTT TGCGTCCATC CAAAAAAAAA AGTAAGAATT TTTGAAAATT
  901 CAATATAAAT GACAGCTCAG TTACAAAGTG AAAGTGACCT GCAGGCATGC AAGCTCTGCA
  961 TGTGTCAGAG GTTTTCACCG TCATCACCGA AACGCGCGAG ACGAAAGGGC CCTCGTGATA
 1021 CGCCCTATTT TTTATAGGTT AATGTCATGA ATAATAAATG GTTTTCTTAG GGTCCTTTTC
 1081 ATCACGTGCT AATAAAAATA TTATAATTTT AATTTTTTTA ATATCACTAT ATAAGTAAAA
1141 TAGGAAAGGT AAAAAAAGAA CTAAAAGCAA AAAATTAGGC TTCTG
//
Primer: Amp_R
Sequence file: RDB12344_A3Lkb.seq check
>01957_12344_A3Lk_Amp_R_G10_19.ab1
    1 TTGCTTCATA TTATTGAGCA TTTATCAGGG TTATTGTCTC ATGAGCGGAT ACATATTTGA
   61 ATGTATTTAG AAAAATAAAC AAATAGGGGT TCCGCGCACA TTTCCCCGAA AAGTGCCACC
  121 TGACGGATCG CTTGCCTGTA ACTTACACGC GCCTCGTATC TTTTAATGAT GGAATAATTT
  181 GGGAATTTAC TCTGTGTTTA TTTATTTTTA TGTTTTGTAT TTGGATTTTA GAAAGTAAAT
  241 AAAGAAGGTA GAAGAGTTAC GGAATGAAGA AAAAAAAATA AACAAAGGTT TAAAAAATTT
  301 CAACAAAAAG CGTACTTTAC ATATATATTT ATTAGACAAG AAAAGCAGAT TAAATAGATA
  361 TACATTCGAT TAACGATAAG TAAAATGTAA AATCACAGGA TTTTCGTGTG TGGTCTTCTA
  421 CACAGACAAG ATGAAACAAT TCGGCATTAA TACCTGAGAG CAGGAAGAGC AAGATAAAAG
  481 GTAGTATTTG TTGGCGATCC CCCTAGAGTC TTTTACATCT TCGGAAAACA AAAACTATTT
  541 TTTCTTTAAT TTCTTTTTTT ACTTTCTATT TTTAATTTAT ATATTTATAT TAAAAAATTT
  601 AAATTATAAT TATTTTTATA GCACGTGATG AAAAGGACCC TAAGAAACCA TTATTATCAT
  661 GACATTAACC TATAAAAATA GGCGTATCAC GAGGCCCTTT CGTCTCGCGC GTTTCGGTGA
  721 TGACGGTGAA AACCTCTGAC ACATGCAGAG CTTGCATGCC TGCAGGTCAC TTTCACTTTG
  781 TAACTGAGCT GTCATTTATA TTGAATTTTC AAAAATTCTT ACTTTTTTTT TGGATGGACG
  841 CAAAGAAGTT TAATAATCAT ATTACATGGC ATTACCACCA TATACATATC CATATACATA
  901 TCATATCTAT CTACTAATAT GTGGTGGAAA TGTAAGAGCT CCATTATTCT TAGCTAAAAA
  961 AACTTCTTCT TTGGACTTCA GTATACCGCT ACTGCTCATG CTATATTGAG TACGATAAAG
 1021 CGGCGACCGG TGAACGCCCT CGAGGAGACT CTCCTCGTGC GTCTCTCCTA CCGATCCGAT
1081 CTGAACGCAT GTGACCTCGG CGACTGATCC GA
//
Primer: LEU2pro_F
Sequence file: RDB12344_A3Lkc.seq check
>01957_12344_A3Lk_LEU2pro_F_H10_22.ab1
    1 CACTTGGCAG TTGGACAATA TTTATTTAAG GACCTATTGT TTTTTCCAAT TAGCCTGGTT
   61 AGCAATCGTC TTACTTTCTA ACTTTTCTTA CCTTTTACAT TTCAGCAATA TATATATATA
  121 TATTTCAAGG ATATACCATT CTAATGTCTG CCCCTAAGAA GATCGTCGTT TTGCCAGGTG
  181 ACCACGTTGG TCAAGAAATC ACAGCCGAAG CCATTAAGGT TCTTAAAGCT ATTTCTGATG
  241 TTCGTTCCAA TGTCAAGTTC GATTTCGAAA ATCATTTAAT TGGTGGTGCT GCTATCGATG
  301 CTACAGGTGT TCCACTTCCA GATGAGGCGC TGGAAGCCTC CAAGAAGGCT GATGCCGTTT
  361 TGTTAGGTGC TGTGGGTGGT CCTAAATGGG GTACAGGTAG TGTTAGACCT GAACAAGGTT
  421 TACTAAAAAT CCGTAAAGAA CTTCAATTGT ACGCCAACTT AAGACCATGT AACTTTGCAT
  481 CCGACTCTCT TTTAGACTTA TCTCCAATCA AGCCACAATT TGCTAAAGGT ACTGACTTCG
  541 TTGTTGTCAG AGAATTAGTG GGAGGTATTT ACTTTGGTAA GAGAAAGGAA GACGATGGTG
  601 ATGGTGTCGC TTGGGATAGT GAACAATACA CCGTTCCAGA AGTGCAAAGA ATCACAAGAA
  661 TGGCCGCTTT CATGGCCCTA CAACATGAGC CACCATTGCC TATTTGGTCC TTGGATAAAG
  721 CTAATGTTTT GGCCTCTTCA AGATTATGGA GAAAAACTGT GGAGGAAACC ATCAAGAACG
  781 AATTTCCTAC ATTGAAGGTT CAACATCAAT TGATTGATTC TGCCGCCATG ATCCTAGTTA
  841 AGAACCCAAA CCCACCTAAA TGGTATTATA ATCACCAGCA ACATGTTTGG TGATATCATC
  901 TCCGATGAAG CCTCCGTTAT CCCAGGTTCC TTGGGTTTGT TGCCATCTGC GTCCTTGGCC
  961 TCTTTGCCAG ACAAGAACAC CGCATTTGGG TTTGTACGAA CCATGCCACG GGTTTCTGCT
 1021 CCAGATTTTG CCAAGAATAA GGTCAACCCC TATCGCCACT ATCTTGTCTG CTGCAATGAT
 1081 GTTGAAAATG TCAATTGACT TGCTGAAGAG TAGCATGAAG ATGCCAGTAA AAGGTTTGGA
1141 TGCAAGGTAT ATCAAGAAAC ACAC
//

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

original Iha, H., Shuttle-vector system for Saccharomyces cerevisiae designed to produce C-terminal-Myc-tagged fusion proteins. Biotechniques, 25, 936-938 (1998). PMID 9863043. [link to RRC of NBRP]
user_report Yoshimoto, N., Cytokine-dependent activation of JAK-STAT pathway in Saccharomyces cerevisiae. Biotechnol. Bioeng. 113 (8): 1796-1804 (2016). PMID 26853220. [link to RRC of NBRP]