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pGML10

E. coli - S. cerevisiae shuttle vector

Catalog number RDB01957
Resource name pGML10
Alternative name E. coli-S. cerevisiae shuttle vector
Clone info. E. coli-S. cerevisiae shuttle vector, 9E10 epitope (Myc-tag), centromere-type ori, LEU2, GAL1-10 promoter, Ampicillin
Vector backbone pGML10 (Plasmid)
Size of vector backbone 5,446 bp
Selectable markers Amp^r (E. coli), LEU2 (S. cerevisiae)
Depositor|Developer Iha, Hidekatsu |
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External Database

            Reference sequence
              

            Distribution information

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            RDB01957 pGML10 DNA solution Please contact us.

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            Please review the QC test results indicated by check icon below as well as clone information before placing your order.

            References and tips

            Electronic file

            Sequence RDB01957z.seq
            Remarks, protocol and/or map (gif) RDB01957.gif
            Remarks, protocol and/or map (gif) RDB01957z.gif

            Original reference

            original Iha, H., Shuttle-vector system for Saccharomyces cerevisiae designed to produce C-terminal-Myc-tagged fusion proteins. Biotechniques, 25, 936-938 (1998). PMID 9863043.

            Further references such as user reports and related articles (go to bottom)

            Featured content

            Featured content Expression Vector Backbone of Saccharomyces cerevisiae (English text)

            Sequence information

            RIKEN BRC has sequenced portions of this material for quality test.
            Please review the QC test results indicated by check icon as well as clone information before placing your order.

            Test sheet RDB12344_A3Lk.pdf check

            Nucleotide sequence of a portion of this resource (if available).

            Primer: ADHrev
            Sequence file: RDB12344_A3Lka.seq check
            >01957_12344_A3Lk_ADHrev_A12_03.ab1
                1 TTAGGGATTA ATCAGGAATA AGATTTTCGT TTTAAACCTA AGAGTCACTT TAAAATTTGT
               61 ATACACTTAT TTTTTTTATA ACTTATTTAA TAATAAAAAT CATAAATCAT AAGAAATTCG
              121 CCCGGAATTA GCTTGGCTGC AGGTCGACTC ACAGATCCTC TTCGGAAATC AGTTTCTGTT
              181 CAGAATTCGA GCTCGGTACC CGGGGATCCT CTAGAGTCAA TTCGATCCGG GGTTTTTTCT
              241 CCTTGACGTT AAAGTATAGA GGTATATTAA CAATTTTTTG TTGATACTTT TATTACATTT
              301 GAATAAGAAG TAATACAAAC CGAAAATGTT GAAAGTATTA GTTAAAGTGG TTATGCAGTT
              361 TTTGCATTTA TATATCTGTT AATAGATCAA AAATCATCGC TTCGCTGATT AATTACCCCA
              421 GAAATAAGGC TAAAAAACTA ATCGCATTAT CATCCTATGG TTGTTAATTT GATTCGTTCA
              481 TTTGAAGGTT TGTGGGGCCA GGTTACTGCC AATTTTTCCT CTTCATAACC ATAAAAGCTA
              541 GTATTGTAGA ATCTTTATTG TTCGGAGCAG TGCGGCGCGA GGCACATCTG CGTTTCAGGA
              601 ACGCGACCGG TGAAGACGAG GACGCACGGA GGAGAGTCTT CCTTCGGAGG GCTGTCACCC
              661 GCTCGGCGGC TTCTAATCCG TACTTCAATA TAGCAATGAG CAGTTAAGCG TATTACTGAA
              721 AGTTCCAAAG AGAAGGTTTT TTTAGGCTAA GATAATGGGG CTCTTTACAT TTCCACAACA
              781 TATAAGTAAG ATTAGATATG GATATGTATA TGGATATGTA TATGGTGGTA ATGCCATGTA
              841 ATATGATTAT TAAACTTCTT TGCGTCCATC CAAAAAAAAA AGTAAGAATT TTTGAAAATT
              901 CAATATAAAT GACAGCTCAG TTACAAAGTG AAAGTGACCT GCAGGCATGC AAGCTCTGCA
              961 TGTGTCAGAG GTTTTCACCG TCATCACCGA AACGCGCGAG ACGAAAGGGC CCTCGTGATA
             1021 CGCCCTATTT TTTATAGGTT AATGTCATGA ATAATAAATG GTTTTCTTAG GGTCCTTTTC
             1081 ATCACGTGCT AATAAAAATA TTATAATTTT AATTTTTTTA ATATCACTAT ATAAGTAAAA
            1141 TAGGAAAGGT AAAAAAAGAA CTAAAAGCAA AAAATTAGGC TTCTG
            //
            Primer: Amp_R
            Sequence file: RDB12344_A3Lkb.seq check
            >01957_12344_A3Lk_Amp_R_G10_19.ab1
                1 TTGCTTCATA TTATTGAGCA TTTATCAGGG TTATTGTCTC ATGAGCGGAT ACATATTTGA
               61 ATGTATTTAG AAAAATAAAC AAATAGGGGT TCCGCGCACA TTTCCCCGAA AAGTGCCACC
              121 TGACGGATCG CTTGCCTGTA ACTTACACGC GCCTCGTATC TTTTAATGAT GGAATAATTT
              181 GGGAATTTAC TCTGTGTTTA TTTATTTTTA TGTTTTGTAT TTGGATTTTA GAAAGTAAAT
              241 AAAGAAGGTA GAAGAGTTAC GGAATGAAGA AAAAAAAATA AACAAAGGTT TAAAAAATTT
              301 CAACAAAAAG CGTACTTTAC ATATATATTT ATTAGACAAG AAAAGCAGAT TAAATAGATA
              361 TACATTCGAT TAACGATAAG TAAAATGTAA AATCACAGGA TTTTCGTGTG TGGTCTTCTA
              421 CACAGACAAG ATGAAACAAT TCGGCATTAA TACCTGAGAG CAGGAAGAGC AAGATAAAAG
              481 GTAGTATTTG TTGGCGATCC CCCTAGAGTC TTTTACATCT TCGGAAAACA AAAACTATTT
              541 TTTCTTTAAT TTCTTTTTTT ACTTTCTATT TTTAATTTAT ATATTTATAT TAAAAAATTT
              601 AAATTATAAT TATTTTTATA GCACGTGATG AAAAGGACCC TAAGAAACCA TTATTATCAT
              661 GACATTAACC TATAAAAATA GGCGTATCAC GAGGCCCTTT CGTCTCGCGC GTTTCGGTGA
              721 TGACGGTGAA AACCTCTGAC ACATGCAGAG CTTGCATGCC TGCAGGTCAC TTTCACTTTG
              781 TAACTGAGCT GTCATTTATA TTGAATTTTC AAAAATTCTT ACTTTTTTTT TGGATGGACG
              841 CAAAGAAGTT TAATAATCAT ATTACATGGC ATTACCACCA TATACATATC CATATACATA
              901 TCATATCTAT CTACTAATAT GTGGTGGAAA TGTAAGAGCT CCATTATTCT TAGCTAAAAA
              961 AACTTCTTCT TTGGACTTCA GTATACCGCT ACTGCTCATG CTATATTGAG TACGATAAAG
             1021 CGGCGACCGG TGAACGCCCT CGAGGAGACT CTCCTCGTGC GTCTCTCCTA CCGATCCGAT
            1081 CTGAACGCAT GTGACCTCGG CGACTGATCC GA
            //
            Primer: LEU2pro_F
            Sequence file: RDB12344_A3Lkc.seq check
            >01957_12344_A3Lk_LEU2pro_F_H10_22.ab1
                1 CACTTGGCAG TTGGACAATA TTTATTTAAG GACCTATTGT TTTTTCCAAT TAGCCTGGTT
               61 AGCAATCGTC TTACTTTCTA ACTTTTCTTA CCTTTTACAT TTCAGCAATA TATATATATA
              121 TATTTCAAGG ATATACCATT CTAATGTCTG CCCCTAAGAA GATCGTCGTT TTGCCAGGTG
              181 ACCACGTTGG TCAAGAAATC ACAGCCGAAG CCATTAAGGT TCTTAAAGCT ATTTCTGATG
              241 TTCGTTCCAA TGTCAAGTTC GATTTCGAAA ATCATTTAAT TGGTGGTGCT GCTATCGATG
              301 CTACAGGTGT TCCACTTCCA GATGAGGCGC TGGAAGCCTC CAAGAAGGCT GATGCCGTTT
              361 TGTTAGGTGC TGTGGGTGGT CCTAAATGGG GTACAGGTAG TGTTAGACCT GAACAAGGTT
              421 TACTAAAAAT CCGTAAAGAA CTTCAATTGT ACGCCAACTT AAGACCATGT AACTTTGCAT
              481 CCGACTCTCT TTTAGACTTA TCTCCAATCA AGCCACAATT TGCTAAAGGT ACTGACTTCG
              541 TTGTTGTCAG AGAATTAGTG GGAGGTATTT ACTTTGGTAA GAGAAAGGAA GACGATGGTG
              601 ATGGTGTCGC TTGGGATAGT GAACAATACA CCGTTCCAGA AGTGCAAAGA ATCACAAGAA
              661 TGGCCGCTTT CATGGCCCTA CAACATGAGC CACCATTGCC TATTTGGTCC TTGGATAAAG
              721 CTAATGTTTT GGCCTCTTCA AGATTATGGA GAAAAACTGT GGAGGAAACC ATCAAGAACG
              781 AATTTCCTAC ATTGAAGGTT CAACATCAAT TGATTGATTC TGCCGCCATG ATCCTAGTTA
              841 AGAACCCAAA CCCACCTAAA TGGTATTATA ATCACCAGCA ACATGTTTGG TGATATCATC
              901 TCCGATGAAG CCTCCGTTAT CCCAGGTTCC TTGGGTTTGT TGCCATCTGC GTCCTTGGCC
              961 TCTTTGCCAG ACAAGAACAC CGCATTTGGG TTTGTACGAA CCATGCCACG GGTTTCTGCT
             1021 CCAGATTTTG CCAAGAATAA GGTCAACCCC TATCGCCACT ATCTTGTCTG CTGCAATGAT
             1081 GTTGAAAATG TCAATTGACT TGCTGAAGAG TAGCATGAAG ATGCCAGTAA AAGGTTTGGA
            1141 TGCAAGGTAT ATCAAGAAAC ACAC
            //

            Please visit Sequencing and PCR primers for primer information.


            References

            Original, user report and related articles

            original Iha, H., Shuttle-vector system for Saccharomyces cerevisiae designed to produce C-terminal-Myc-tagged fusion proteins. Biotechniques, 25, 936-938 (1998). PMID 9863043.
            user report Yoshimoto, N., Cytokine-dependent activation of JAK-STAT pathway in Saccharomyces cerevisiae. Biotechnol. Bioeng. 113 (8): 1796-1804 (2016). PMID 26853220.

            2022.02.09

            GNP_filter3_RDBDEP_html_220112.pl