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pGMH20

E. coli - S. cerevisiae shuttle vector

Catalog number RDB01956
Resource name pGMH20
Alternative name E. coli-S. cerevisiae shuttle vector
Clone info. E. coli-S. cerevisiae shuttle vector, 9E10 epitope (Myc-tag), 2micron ori, HIS3, GAL1-10 promoter, Ampicillin
Vector backbone pGMH20 (Plasmid)
Size of vector backbone 5,609 bp
Selectable markers Amp^r (E. coli), HIS3 (S. cerevisiae)
Depositor|Developer Iha, Hidekatsu |
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External Database

            Reference sequence
              

            Distribution information

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            RDB01956 pGMH20 DNA solution Please contact us.

            Ordering Information [in Japanese] [in English]
            Please review the QC test results indicated by check icon below as well as clone information before placing your order.

            References and tips

            Electronic file

            Sequence RDB01956z.seq
            Remarks, protocol and/or map (gif) RDB01956.gif
            Remarks, protocol and/or map (gif) RDB01956z.gif

            Original reference

            original Iha, H., Shuttle-vector system for Saccharomyces cerevisiae designed to produce C-terminal-Myc-tagged fusion proteins. Biotechniques, 25, 936-938 (1998). PMID 9863043.

            Further references such as user reports and related articles (go to bottom)

            Featured content

            Featured content Dnaconda's recommendation EXR0004j (Japanese text)
            Featured content Expression Vector Backbone of Saccharomyces cerevisiae (English text)

            Sequence information

            RIKEN BRC has sequenced portions of this material for quality test.
            Please review the QC test results indicated by check icon as well as clone information before placing your order.

            Test sheet RDB13123_A5Bg.pdf check

            Nucleotide sequence of a portion of this resource (if available).

            Primer: ADH_rev
            Sequence file: RDB13123_A5Bga.seq check
            >01956_13123_A5Bg_ADHrev_A11_02.ab1
                1 GAACTAATCA GAATAAGATT TTCGTTTTAA ACCTAAGAGT CACTTTAAAA TTTGTATACA
               61 CTTATTTTTT TTATAACTTA TTTAATAATA AAAATCATAA ATCATAAGAA ATTCGCCCGG
              121 AATTAGCTTG GCTGCAGGTC GACTCACAGA TCCTCTTCGG AAATCAGTTT CTGTTCAGAA
              181 TTCGAGCTCG GTACCCGGGG ATCCTCTAGA GTCAATTCGA TCCGGGGTTT TTTCTCCTTG
              241 ACGTTAAAGT ATAGAGGTAT ATTAACAATT TTTTGTTGAT ACTTTTATTA CATTTGAATA
              301 AGAAGTAATA CAAACCGAAA ATGTTGAAAG TATTAGTTAA AGTGGTTATG CAGTTTTTGC
              361 ATTTATATAT CTGTTAATAG ATCAAAAATC ATCGCTTCGC TGATTAATTA CCCCAGAAAT
              421 AAGGCTAAAA AACTAATCGC ATTATCATCC TATGGTTGTT AATTTGATTC GTTCATTTGA
              481 AGGTTTGTGG GGCCAGGTTA CTGCCAATTT TTCCTCTTCA TAACCATAAA AGCTAGTATT
              541 GTAGAATCTT TATTGTTCGG AGCAGTGCGG CGCGAGGCAC ATCTGCGTTT CAGGAACGCG
              601 ACCGGTGAAG ACGAGGACGC ACGGAGGAGA GTCTTCCTTC GGAGGGCTGT CACCCGCTCG
              661 GCGGCTTCTA ATCCGTACTT CAATATAGCA ATGAGCAGTT AAGCGTATTA CTGAAAGTTC
              721 CAAAGAGAAG GTTTTTTTAG GCTAAGATAA TGGGGCTCTT TACATTTCCA CAACATATAA
              781 GTAAGATTAG ATATGGATAT GTATATGGAT ATGTATATGG TGGTAATGCC ATGTAATATG
              841 ATTATTAAAC TTCTTTGCGT CCATCCAAAA AAAAAAGTAA GAATTTTTTG AAAATTCAAT
              901 ATAAATGACA GCTCAGTTAC AAAGTGAAAG TGACCTGCAG GCATGCAAGC AACGAAAGCA
              961 TCTGTGCTTC ATTTTGTAGA ACAAAAATGC AACGCGAGAG CGCTAATTTT TTCAACAAGA
             1021 ATCTGAGCTG CCATTTTTAC AGAACAGAAA TGCAACGCGA AGCGCTATTT TTACTACGAG
            1081 ATCTGTGCTC ATTTTGGTAA ACCAAAAAAT GCAAACGCCG TAGGAGT
            //
            Primer: Amp_R
            Sequence file: RDB13123_A5Bgb.seq check
            >01956_13123_A5Bg_Amp_R_B11_05.ab1
                1 TCGTTTCATA TTATTGAGCA TTTATCAGGG TTATTGTCTC ATGAGCGGAT ACATATTTGA
               61 ATGTATTTAG AAAAATAAAC AAATAGGGGT TCCGCGCACA TTTCCCCGAA AAGTGCCACC
              121 TGACGTCTAA GAAACCATTA TTATCATGAC ATTAACCTAT AAAAATAGGC GTATCACGAG
              181 GCCCTTTCGT CTCGCGCGTT TCGGTGATGA CGGTGAAAAC CTCTGACACA TGCAGCTCCC
              241 GGAGACGGTC ACAGCTTGTC TGTAAGCGGA TGCCGGGAGC AGACAAGCCC GTCAGGGCGC
              301 GTCAGCGGGT GTTGGCGGGT GTCGGGGCTG GCTTAACTAT GCGGCATCAG AGCAGATTGT
              361 ACTGAGAGTG CACCATAACG CATTTAAGCA TAAACACGCA CTATGCCGTT CTTCTCATGT
              421 ATATATATAT ACAGGCAACA CGCAGATATA GGTGCGACGT GAACAGTGAG CTGTATGTGC
              481 GCAGCTCGCG TTGCATTTTC GGAAGCGCTC GTTTTCGGAA ACGCTTTGAA GTTCCTATTC
              541 CGAAGTTCCT ATTCTCTAGC TAGAAAGTAT AGGAACTTCA GAGCGCTTTT GAAAACCAAA
              601 AGCGCTCTGA AGACGCACTT TCAAAAAACC AAAAACGCAC CGGACTGTAA CGAGCTACTA
              661 AAATATTGCG AATACCGCTT CCACAAACAT TGCTCAAAAG TATCTCTTTG CTATATATCT
              721 CTGTGCTATA TCCCTATATA ACCTACCCAT CCACCTTTCG CTCCTTGAAC TTGCATCTAA
              781 ACTCGACCTC TACATTTTTT TATGTTTATC TCTAGTATTA CTCTTTAGAC AAAAAAATTG
              841 TAGTAAGAAC TATTCATAGA GTGAATCGAA AACAATACGA AAATGTAAAC ATTTCCTATA
              901 CGTAGTATAT AGAGACAAAA TAGAAGAAAC CGTTCATAAT TTTCTGACCA ATGAAGAATC
              961 ATCAACGCTA TCACTTTCTG TTCACAAAGT ATGCGCAATC CCACATCGGG TATAGAATAT
             1021 AATCGGGGAT GCCCTTTATC TTGAAAAAAT GCACCGCAGC TTCGCTAGTT ATTCAGTTAA
             1081 ACGCGGCAGT GAGTTCAGCT TTTTTTATGC AGAGAAACGA CCTAAGCTAG CTTCGTTCGT
            1141 AAGCATTTAG
            //
            Primer: HIS3pro_F
            Sequence file: RDB13123_A5Bgc.seq check
            >01956_13123_A5Bg_HIS3pro_F_C11_08.ab1
                1 TGCATATACA TTATATAAAG TAATGTGATT TCTTCGAAGA ATATACTAAA AAATGAGCAG
               61 GCAAGATAAA CGAAGGCAAA GATGACAGAG CAGAAAGCCC TAGTAAAGCG TATTACAAAT
              121 GAAACCAAGA TTCAGATTGC GATCTCTTTA AAGGGTGGTC CCCTAGCGAT AGAGCACTCG
              181 ATCTTCCCAG AAAAAGAGGC AGAAGCAGTA GCAGAACAGG CCACACAATC GCAAGTGATT
              241 AACGTCCACA CAGGTATAGG GTTTCTGGAC CATATGATAC ATGCTCTGGC CAAGCATTCC
              301 GGCTGGTCGC TAATCGTTGA GTGCATTGGT GACTTACACA TAGACGACCA TCACACCACT
              361 GAAGACTGCG GGATTGCTCT CGGTCAAGCA TTTAAAGAGG CCCTAGGGGC CGTGCGTGGA
              421 GTAAAAAGGT TTGGATCAGG ATTTGCGCCT TTGGATGAGG CACTTTCCAG AGCGGTGGTA
              481 GATCTTTCGA ACAGGCCGTA CGCAGTTGTC GAACTTGGTT TGCAGAGGGA GAAAGTAGGA
              541 GATCTCTCTT GCGAGATGAT CCCGCATTTT CTTGAAAGTT TTGCAGAGGC AAGCAGAATT
              601 ACCCTCCACG TTGATTGTCT GCGAGGCAAG AATGATCATC ACCGTAGTGA GAGTGCGTTC
              661 AAGGCTCTTG CGGTTGCCAT AAGAGAAGCC ACCTCGCCCA ATGGAACCAA CGATGTTCCC
              721 TCCACCAAAG GTGTTCTTAT GTAGTGACAC CGATTATTTA AAGCGGCAAG CTTACATTTT
              781 ATGTTAGCTG GTGGACTGAC GCCAGAAAAT GTTGGTGATG CGCTTAGATT AAATGGCGTT
              841 ATTGGTGTTG ATGTAAGCGG AGGTGTGGAG ACAAATGGTG TAAAAGACTC TAACAAAATA
              901 GCAAATTTCG TCAAAAATGC TAAGAAATAG GTTATTACTG AGTAGTATTT ATTTAAGTAT
              961 TGTTTGTGCA CTTGCCGGTA GAGGTGTGGT CAATAAGAGC GACCTCATGC TATACCCTGA
             1021 GAAAGCAACC CTGACCCTAC AGGAAAGAGT TTACTCAAGA ATAAAGATTT TTCGTTTTAA
             1081 AACCTAAGAG TTCAGTTAAA ATTTGTATAC ACTTACTCTC TAATAAACCT TATATTTTAA
            1141 ATA
            //

            Please visit Sequencing and PCR primers for primer information.


            References

            Original, user report and related articles

            original Iha, H., Shuttle-vector system for Saccharomyces cerevisiae designed to produce C-terminal-Myc-tagged fusion proteins. Biotechniques, 25, 936-938 (1998). PMID 9863043.
            user report Yoshimoto, N., Cytokine-dependent activation of JAK-STAT pathway in Saccharomyces cerevisiae. Biotechnol. Bioeng. 113 (8): 1796-1804 (2016). PMID 26853220.
            user report Yoshimoto, N., High-throughput de novo screening of receptor agonists with an automated single-cell analysis and isolation system. Sci Rep., 4: 4242 (2014). PMID 24577528.

            2022.02.09

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