RDB01956 - pGMH20

Please review the QC test results indicated by check

icon below as well as clone information before placing your order.

pGMH20 (#RDB01956)

E. coli - S. cerevisiae shuttle vector

Alternative name	E. coli-S. cerevisiae shuttle vector
Clone info.	E. coli-S. cerevisiae shuttle vector, 9E10 epitope (Myc-tag), 2micron ori, HIS3, GAL1-10 promoter, Ampicillin
Vector backbone	pGMH20 (Plasmid)
Size of vector backbone	5,609 bp
Selectable markers	Amp^r (E. coli), HIS3 (S. cerevisiae)
Depositor\|Developer	Iha, Hidekatsu \|

Sequence	RDB01956z.seq
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Ordering forms	Order form [Credit Card Payment] [Bank Transfer Payment] [Example of order form ] MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ] Please visit Information of Request for Distribution.[link] For for-profit-research purpose, please contact us.
Terms and conditions for distribution	Acknowledgement to Depositor and Developer in publication.

必要書類	提供依頼書 [依頼書の記入例 ] 提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ] 手続きの概要は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。
MTAに書く使用条件	研究結果の発表時、謝辞に寄託者および樹立者名を入れること。

Catalog #	Resource name	Shipping form	Fee (non-profit org.)
RDB01956	pGMH20	DNA solution	Please contact us.

Please review the QC test results indicated by check

icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The pGMH20 was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB01956).

Reference section:

Iha, H., Tsurugi, K., Shuttle-vector system for Saccharomyces cerevisiae designed to produce C-terminal-Myc-tagged fusion proteins. Biotechniques, 25, 936-938 (1998). PMID 9863043. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet	RDB13123_A5Bg.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: ADH_rev Sequence file: RDB13123_A5Bga.seq
>01956_13123_A5Bg_ADHrev_A11_02.ab1 1 GAACTAATCA GAATAAGATT TTCGTTTTAA ACCTAAGAGT CACTTTAAAA TTTGTATACA 61 CTTATTTTTT TTATAACTTA TTTAATAATA AAAATCATAA ATCATAAGAA ATTCGCCCGG 121 AATTAGCTTG GCTGCAGGTC GACTCACAGA TCCTCTTCGG AAATCAGTTT CTGTTCAGAA 181 TTCGAGCTCG GTACCCGGGG ATCCTCTAGA GTCAATTCGA TCCGGGGTTT TTTCTCCTTG 241 ACGTTAAAGT ATAGAGGTAT ATTAACAATT TTTTGTTGAT ACTTTTATTA CATTTGAATA 301 AGAAGTAATA CAAACCGAAA ATGTTGAAAG TATTAGTTAA AGTGGTTATG CAGTTTTTGC 361 ATTTATATAT CTGTTAATAG ATCAAAAATC ATCGCTTCGC TGATTAATTA CCCCAGAAAT 421 AAGGCTAAAA AACTAATCGC ATTATCATCC TATGGTTGTT AATTTGATTC GTTCATTTGA 481 AGGTTTGTGG GGCCAGGTTA CTGCCAATTT TTCCTCTTCA TAACCATAAA AGCTAGTATT 541 GTAGAATCTT TATTGTTCGG AGCAGTGCGG CGCGAGGCAC ATCTGCGTTT CAGGAACGCG 601 ACCGGTGAAG ACGAGGACGC ACGGAGGAGA GTCTTCCTTC GGAGGGCTGT CACCCGCTCG 661 GCGGCTTCTA ATCCGTACTT CAATATAGCA ATGAGCAGTT AAGCGTATTA CTGAAAGTTC 721 CAAAGAGAAG GTTTTTTTAG GCTAAGATAA TGGGGCTCTT TACATTTCCA CAACATATAA 781 GTAAGATTAG ATATGGATAT GTATATGGAT ATGTATATGG TGGTAATGCC ATGTAATATG 841 ATTATTAAAC TTCTTTGCGT CCATCCAAAA AAAAAAGTAA GAATTTTTTG AAAATTCAAT 901 ATAAATGACA GCTCAGTTAC AAAGTGAAAG TGACCTGCAG GCATGCAAGC AACGAAAGCA 961 TCTGTGCTTC ATTTTGTAGA ACAAAAATGC AACGCGAGAG CGCTAATTTT TTCAACAAGA 1021 ATCTGAGCTG CCATTTTTAC AGAACAGAAA TGCAACGCGA AGCGCTATTT TTACTACGAG 1081 ATCTGTGCTC ATTTTGGTAA ACCAAAAAAT GCAAACGCCG TAGGAGT //
Primer: Amp_R Sequence file: RDB13123_A5Bgb.seq
>01956_13123_A5Bg_Amp_R_B11_05.ab1 1 TCGTTTCATA TTATTGAGCA TTTATCAGGG TTATTGTCTC ATGAGCGGAT ACATATTTGA 61 ATGTATTTAG AAAAATAAAC AAATAGGGGT TCCGCGCACA TTTCCCCGAA AAGTGCCACC 121 TGACGTCTAA GAAACCATTA TTATCATGAC ATTAACCTAT AAAAATAGGC GTATCACGAG 181 GCCCTTTCGT CTCGCGCGTT TCGGTGATGA CGGTGAAAAC CTCTGACACA TGCAGCTCCC 241 GGAGACGGTC ACAGCTTGTC TGTAAGCGGA TGCCGGGAGC AGACAAGCCC GTCAGGGCGC 301 GTCAGCGGGT GTTGGCGGGT GTCGGGGCTG GCTTAACTAT GCGGCATCAG AGCAGATTGT 361 ACTGAGAGTG CACCATAACG CATTTAAGCA TAAACACGCA CTATGCCGTT CTTCTCATGT 421 ATATATATAT ACAGGCAACA CGCAGATATA GGTGCGACGT GAACAGTGAG CTGTATGTGC 481 GCAGCTCGCG TTGCATTTTC GGAAGCGCTC GTTTTCGGAA ACGCTTTGAA GTTCCTATTC 541 CGAAGTTCCT ATTCTCTAGC TAGAAAGTAT AGGAACTTCA GAGCGCTTTT GAAAACCAAA 601 AGCGCTCTGA AGACGCACTT TCAAAAAACC AAAAACGCAC CGGACTGTAA CGAGCTACTA 661 AAATATTGCG AATACCGCTT CCACAAACAT TGCTCAAAAG TATCTCTTTG CTATATATCT 721 CTGTGCTATA TCCCTATATA ACCTACCCAT CCACCTTTCG CTCCTTGAAC TTGCATCTAA 781 ACTCGACCTC TACATTTTTT TATGTTTATC TCTAGTATTA CTCTTTAGAC AAAAAAATTG 841 TAGTAAGAAC TATTCATAGA GTGAATCGAA AACAATACGA AAATGTAAAC ATTTCCTATA 901 CGTAGTATAT AGAGACAAAA TAGAAGAAAC CGTTCATAAT TTTCTGACCA ATGAAGAATC 961 ATCAACGCTA TCACTTTCTG TTCACAAAGT ATGCGCAATC CCACATCGGG TATAGAATAT 1021 AATCGGGGAT GCCCTTTATC TTGAAAAAAT GCACCGCAGC TTCGCTAGTT ATTCAGTTAA 1081 ACGCGGCAGT GAGTTCAGCT TTTTTTATGC AGAGAAACGA CCTAAGCTAG CTTCGTTCGT 1141 AAGCATTTAG //
Primer: HIS3pro_F Sequence file: RDB13123_A5Bgc.seq
>01956_13123_A5Bg_HIS3pro_F_C11_08.ab1 1 TGCATATACA TTATATAAAG TAATGTGATT TCTTCGAAGA ATATACTAAA AAATGAGCAG 61 GCAAGATAAA CGAAGGCAAA GATGACAGAG CAGAAAGCCC TAGTAAAGCG TATTACAAAT 121 GAAACCAAGA TTCAGATTGC GATCTCTTTA AAGGGTGGTC CCCTAGCGAT AGAGCACTCG 181 ATCTTCCCAG AAAAAGAGGC AGAAGCAGTA GCAGAACAGG CCACACAATC GCAAGTGATT 241 AACGTCCACA CAGGTATAGG GTTTCTGGAC CATATGATAC ATGCTCTGGC CAAGCATTCC 301 GGCTGGTCGC TAATCGTTGA GTGCATTGGT GACTTACACA TAGACGACCA TCACACCACT 361 GAAGACTGCG GGATTGCTCT CGGTCAAGCA TTTAAAGAGG CCCTAGGGGC CGTGCGTGGA 421 GTAAAAAGGT TTGGATCAGG ATTTGCGCCT TTGGATGAGG CACTTTCCAG AGCGGTGGTA 481 GATCTTTCGA ACAGGCCGTA CGCAGTTGTC GAACTTGGTT TGCAGAGGGA GAAAGTAGGA 541 GATCTCTCTT GCGAGATGAT CCCGCATTTT CTTGAAAGTT TTGCAGAGGC AAGCAGAATT 601 ACCCTCCACG TTGATTGTCT GCGAGGCAAG AATGATCATC ACCGTAGTGA GAGTGCGTTC 661 AAGGCTCTTG CGGTTGCCAT AAGAGAAGCC ACCTCGCCCA ATGGAACCAA CGATGTTCCC 721 TCCACCAAAG GTGTTCTTAT GTAGTGACAC CGATTATTTA AAGCGGCAAG CTTACATTTT 781 ATGTTAGCTG GTGGACTGAC GCCAGAAAAT GTTGGTGATG CGCTTAGATT AAATGGCGTT 841 ATTGGTGTTG ATGTAAGCGG AGGTGTGGAG ACAAATGGTG TAAAAGACTC TAACAAAATA 901 GCAAATTTCG TCAAAAATGC TAAGAAATAG GTTATTACTG AGTAGTATTT ATTTAAGTAT 961 TGTTTGTGCA CTTGCCGGTA GAGGTGTGGT CAATAAGAGC GACCTCATGC TATACCCTGA 1021 GAAAGCAACC CTGACCCTAC AGGAAAGAGT TTACTCAAGA ATAAAGATTT TTCGTTTTAA 1081 AACCTAAGAG TTCAGTTAAA ATTTGTATAC ACTTACTCTC TAATAAACCT TATATTTTAA 1141 ATA //

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

original	Iha, H., Shuttle-vector system for Saccharomyces cerevisiae designed to produce C-terminal-Myc-tagged fusion proteins. Biotechniques, 25, 936-938 (1998). PMID 9863043. [link to RRC of NBRP]
user_report	Yoshimoto, N., Cytokine-dependent activation of JAK-STAT pathway in Saccharomyces cerevisiae. Biotechnol. Bioeng. 113 (8): 1796-1804 (2016). PMID 26853220. [link to RRC of NBRP]
user_report	Yoshimoto, N., High-throughput de novo screening of receptor agonists with an automated single-cell analysis and isolation system. Sci Rep., 4: 4242 (2014). PMID 24577528. [link to RRC of NBRP]

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