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pGMH10

E. coli - S. cerevisiae shuttle vector

Catalog number RDB01955
Resource name pGMH10
Alternative name E. coli-S. cerevisiae shuttle vector
Clone info. E. coli-S. cerevisiae shuttle vector, 9E10 epitope (Myc-tag), centromere-type ori, HIS3, GAL1-10 promoter, Ampicillin
Vector backbone pGMH10 (Plasmid)
Size of vector backbone 4,817 bp
Selectable markers Amp^r (E. coli), HIS3 (S. cerevisiae)
Depositor|Developer Iha, Hidekatsu |
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External Database

            Reference sequence
              

            Distribution information

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            RDB01955 pGMH10 DNA solution Please contact us.

            Ordering Information [in Japanese] [in English]
            Please review the QC test results indicated by check icon below as well as clone information before placing your order.

            References and tips

            Electronic file

            Sequence RDB01955z.seq
            Remarks, protocol and/or map (gif) RDB01955.gif
            Remarks, protocol and/or map (gif) RDB01955z.gif

            Original reference

            original Iha, H., Shuttle-vector system for Saccharomyces cerevisiae designed to produce C-terminal-Myc-tagged fusion proteins. Biotechniques, 25, 936-938 (1998). PMID 9863043.

            Further references such as user reports and related articles (go to bottom)

            Featured content

            Featured content Expression Vector Backbone of Saccharomyces cerevisiae (English text)

            Sequence information

            RIKEN BRC has sequenced portions of this material for quality test.
            Please review the QC test results indicated by check icon as well as clone information before placing your order.

            Test sheet RDB13033_A4L8.pdf check

            Nucleotide sequence of a portion of this resource (if available).

            Primer: ADHrev
            Sequence file: RDB13033_A4L8a.seq check
            >01955_13033_A4L8_ADHrev_A04_01.ab1
                1 GAAGGGTTCT CAAGATAAGA ATTTTCGTTT TAAAACCTAA GAGTCACTTT AAAATTTGTA
               61 TACACTTATT TTTTTTATAA CTTATTTAAT AATAAAAATC ATAAATCATA AGAAATTCGC
              121 CCGGAATTAG CTTGGCTGCA GGTCGACTCA CAGATCCTCT TCGGAAATCA GTTTCTGTTC
              181 AGAATTCGAG CTCGGTACCC GGGGATCCTC TAGAGTCAAT TCGATCCGGG GTTTTTTCTC
              241 CTTGACGTTA AAGTATAGAG GTATATTAAC AATTTTTTGT TGATACTTTT ATTACATTTG
              301 AATAAGAAGT AATACAAACC GAAAATGTTG AAAGTATTAG TTAAAGTGGT TATGCAGTTT
              361 TTGCATTTAT ATATCTGTTA ATAGATCAAA AATCATCGCT TCGCTGATTA ATTACCCCAG
              421 AAATAAGGCT AAAAAACTAA TCGCATTATC ATCCTATGGT TGTTAATTTG ATTCGTTCAT
              481 TTGAAGGTTT GTGGGGCCAG GTTACTGCCA ATTTTTCCTC TTCATAACCA TAAAAGCTAG
              541 TATTGTAGAA TCTTTATTGT TCGGAGCAGT GCGGCGCGAG GCACATCTGC GTTTCAGGAA
              601 CGCGACCGGT GAAGACGAGG ACGCACGGAG GAGAGTCTTC CTTCGGAGGG CTGTCACCCG
              661 CTCGGCGGCT TCTAATCCGT ACTTCAATAT AGCAATGAGC AGTTAAGCGT ATTACTGAAA
              721 GTTCCAAAGA GAAGGTTTTT TTAGGCTAAG ATAATGGGGC TCTTTACATT TCCACAACAT
              781 ATAAGTAAGA TTAGATATGG ATATGTATAT GGATATGTAT ATGGTGGTAA TGCCATGTAA
              841 TATGATTATT AAACTTCTTT GCGTCCATCC AAAAAAAAAG TAAGAATTTT TGAAAATTCA
              901 ATATAAATGA CAGCTCAGTT ACAAAGTGAA AGTGACCTGC AGGCATGCAA GCTCTGCATG
              961 TGTCAGAGGT TTTCACCGTC ATCACCGAAA CGCGCGAGAC GAAAGGGCCT CGTGATACGC
             1021 CTATTTTTAT AGGTTAATGT CATGATAATA ATGGGTTTTC TTAAGGGTCG TTTTCCATCA
             1081 CGTGCTATAA AAGTAAGTAT AGTTCAAAGT TTCAGTATAA AGTATATAAA ATTAAAAAAT
            1141 TAGGCAG
            //
            Primer: HIS3pro_F
            Sequence file: RDB13033_A4L8b.seq check
            >01955_13033_A4L8_HIS3pro_F_B04_04.ab1
                1 TAGTCAATTA TACATTATAT AAAGTAATGT GATTTCTTCG AAGAATATAC TAAAAAATGA
               61 GCAGGCAAGA TAAACGAAGG CAAAGATGAC AGAGCAGAAA GCCCTAGTAA AGCGTATTAC
              121 AAATGAAACC AAGATTCAGA TTGCGATCTC TTTAAAGGGT GGTCCCCTAG CGATAGAGCA
              181 CTCGATCTTC CCAGAAAAAG AGGCAGAAGC AGTAGCAGAA CAGGCCACAC AATCGCAAGT
              241 GATTAACGTC CACACAGGTA TAGGGTTTCT GGACCATATG ATACATGCTC TGGCCAAGCA
              301 TTCCGGCTGG TCGCTAATCG TTGAGTGCAT TGGTGACTTA CACATAGACG ACCATCACAC
              361 CACTGAAGAC TGCGGGATTG CTCTCGGTCA AGCATTTAAA GAGGCCCTAG GGGCCGTGCG
              421 TGGAGTAAAA AGGTTTGGAT CAGGATTTGC GCCTTTGGAT GAGGCACTTT CCAGAGCGGT
              481 GGTAGATCTT TCGAACAGGC CGTACGCAGT TGTCGAACTT GGTTTGCAGA GGGAGAAAGT
              541 AGGAGATCTC TCTTGCGAGA TGATCCCGCA TTTTCTTGAA AGTTTTGCAG AGGCAAGCAG
              601 AATTACCCTC CACGTTGATT GTCTGCGAGG CAAGAATGAT CATCACCGTA GTGAGAGTGC
              661 GTTCAAGGCT CTTGCGGTTG CCATAAGAGA AGCCACCTCG CCCAATGGAA CCAACGATGT
              721 TCCCTCCACC AAAGGTGTTC TTATGTAGTG ACACCGATTA TTTAAAGCGG CAAGCTTACA
              781 TTTTATGTTA GCTGGTGGAC TGACGCCAGA AAATGTTGGT GATGCGCTTA GATTAAATGG
              841 CGTTATTGGT GTTGATGTAA GCGGAGGTGT GGAGACAAAT GGTGTAAAAG ACTCTAACAA
              901 AATAGCAAAT TTCGTCAAAA ATGCTAAGAA ATAGGTTATT ACTGAGTAGT ATTTATTTAA
              961 GTATTGTTTG TGCACTTGCC GGTAGAGGTG TGGTCAATAA GAGCGACCTC ATGCTATACC
             1021 TGAGAAAGCA ACCCTGACCT ACAGGAAAGA GTTACTCAAG AATAGAATTT TCGTTTTAAA
             1081 ACTAGAGTCA CTTAAATTTG GTATACACTA TTTTTTTATA ACCTTATTTA TTAAATAAAA
            1141 AATCAATTAA AATCA
            //
            Primer: Amp_R
            Sequence file: RDB13033_A4L8c.seq check
            >01955_13033_A4L8_Amp_R_C04_07.ab1
                1 ATTGTTTTAT ATTATTGAGC ATTTATCAGG GTTATTGTCT CATGAGCGGA TACATATTTG
               61 AATGTATTTA GAAAAATAAA CAAATAGGGG TTCCGCGCAC ATTTCCCCGA AAAGTGCCAC
              121 CTGACGGATC GCTTGCCTGT AACTTACACG CGCCTCGTAT CTTTTAATGA TGGAATAATT
              181 TGGGAATTTA CTCTGTGTTT ATTTATTTTT ATGTTTTGTA TTTGGATTTT AGAAAGTAAA
              241 TAAAGAAGGT AGAAGAGTTA CGGAATGAAG AAAAAAAAAT AAACAAAGGT TTAAAAAATT
              301 TCAACAAAAA GCGTACTTTA CATATATATT TATTAGACAA GAAAAGCAGA TTAAATAGAT
              361 ATACATTCGA TTAACGATAA GTAAAATGTA AAATCACAGG ATTTTCGTGT GTGGTCTTCT
              421 ACACAGACAA GATGAAACAA TTCGGCATTA ATACCTGAGA GCAGGAAGAG CAAGATAAAA
              481 GGTAGTATTT GTTGGCGATC CCCCTAGAGT CTTTTACATC TTCGGAAAAC AAAAACTATT
              541 TTTTCTTTAA TTTCTTTTTT TACTTTCTAT TTTTAATTTA TATATTTATA TTAAAAAATT
              601 TAAATTATAA TTATTTTTAT AGCACGTGAT GAAAAGGACC CTAAGAAACC ATTATTATCA
              661 TGACATTAAC CTATAAAAAT AGGCGTATCA CGAGGCCCTT TCGTCTCGCG CGTTTCGGTG
              721 ATGACGGTGA AAACCTCTGA CACATGCAGA GCTTGCATGC CTGCAGGTCA CTTTCACTTT
              781 GTAACTGAGC TGTCATTTAT ATTGAATTTT CAAAAATTCT TACTTTTTTT TTGGATGGAC
              841 GCAAAGAAGT TTAATAATCA TATTACATGG CATTACCACC ATATACATAT CCATATACAT
              901 ATCCATATCT AATCTTACTT ATATGTTGTG GAAATGTAAA GAGCCCCATT ATCTTAGCCT
              961 AAAAAAAAAC CTTCTCTTTG GAAACTTTTC AGTAATACGC TTTAACTGCT CATTTGCTAT
             1021 ATTGAAAGTA CGGGATTAGA AGCCGCCCGA GCGGGTGACA GCCCCTCGGA GAGACTCTCC
             1081 TCCGTGCGTC CTTCGTCTCA ACCGATCGCG ATCTGGAAAC GCAGAATGTT GCCTTCGGCG
            1141 ACCCGGTCA
            //

            Please visit Sequencing and PCR primers for primer information.


            References

            Original, user report and related articles

            original Iha, H., Shuttle-vector system for Saccharomyces cerevisiae designed to produce C-terminal-Myc-tagged fusion proteins. Biotechniques, 25, 936-938 (1998). PMID 9863043.
            user report Shigematsu, M., Evidence for DNA cleavage caused directly by a transfer RNA-targeting toxin. PLoS One. 8 (9): e75512 (2013). PMID 24069426.

            2022.02.09

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