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pGMH10

E. coli - S. cerevisiae shuttle vector

Catalog number RDB01955
Resource name pGMH10
Alternative name E. coli-S. cerevisiae shuttle vector
Clone info. E. coli-S. cerevisiae shuttle vector, 9E10 epitope (Myc-tag), centromere-type ori, HIS3, GAL1-10 promoter, Ampicillin
Vector backbone pGMH10 (Plasmid)
Size of vector backbone 4,817 bp
Selectable markers Amp^r (E. coli), HIS3 (S. cerevisiae)
Depositor Iha, Hidekatsu |

Distribution information

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Terms and conditions set forth by the DEPOSITOR Acknowledgement to Depositor and Developer in publication.
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RDB01955 pGMH10 DNA solution Please contact us.

Ordering Information [in Japanese] [in English]

References and tips

Electronic file

Electronic file Sequence RDB01955z.seq from Depositor
Electronic file Map RDB01955z.gif from Depositor
Electronic file Map RDB01955.gif from Depositor

Featured content

Featured content Expression Vector Backbone of Saccharomyces cerevisiae (English text)

References

original Iha, H., Shuttle-vector system for Saccharomyces cerevisiae designed to produce C-terminal-Myc-tagged fusion proteins. Biotechniques, 25, 936-938 (1998). PMID 9863043.

User reports and related articles (go to bottom)


Sequence information

RIKEN BRC has sequenced portions of this material for quality test. Primers and the results are shown below.

Data are summerized on test sheet below.

Test sheet RDB13033_A4L8.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: ADHrev RDB13033_A4L8a.seq
>01955_13033_A4L8_ADHrev_A04_01.ab1
    1 GAAGGGTTCT CAAGATAAGA ATTTTCGTTT TAAAACCTAA GAGTCACTTT AAAATTTGTA
   61 TACACTTATT TTTTTTATAA CTTATTTAAT AATAAAAATC ATAAATCATA AGAAATTCGC
  121 CCGGAATTAG CTTGGCTGCA GGTCGACTCA CAGATCCTCT TCGGAAATCA GTTTCTGTTC
  181 AGAATTCGAG CTCGGTACCC GGGGATCCTC TAGAGTCAAT TCGATCCGGG GTTTTTTCTC
  241 CTTGACGTTA AAGTATAGAG GTATATTAAC AATTTTTTGT TGATACTTTT ATTACATTTG
  301 AATAAGAAGT AATACAAACC GAAAATGTTG AAAGTATTAG TTAAAGTGGT TATGCAGTTT
  361 TTGCATTTAT ATATCTGTTA ATAGATCAAA AATCATCGCT TCGCTGATTA ATTACCCCAG
  421 AAATAAGGCT AAAAAACTAA TCGCATTATC ATCCTATGGT TGTTAATTTG ATTCGTTCAT
  481 TTGAAGGTTT GTGGGGCCAG GTTACTGCCA ATTTTTCCTC TTCATAACCA TAAAAGCTAG
  541 TATTGTAGAA TCTTTATTGT TCGGAGCAGT GCGGCGCGAG GCACATCTGC GTTTCAGGAA
  601 CGCGACCGGT GAAGACGAGG ACGCACGGAG GAGAGTCTTC CTTCGGAGGG CTGTCACCCG
  661 CTCGGCGGCT TCTAATCCGT ACTTCAATAT AGCAATGAGC AGTTAAGCGT ATTACTGAAA
  721 GTTCCAAAGA GAAGGTTTTT TTAGGCTAAG ATAATGGGGC TCTTTACATT TCCACAACAT
  781 ATAAGTAAGA TTAGATATGG ATATGTATAT GGATATGTAT ATGGTGGTAA TGCCATGTAA
  841 TATGATTATT AAACTTCTTT GCGTCCATCC AAAAAAAAAG TAAGAATTTT TGAAAATTCA
  901 ATATAAATGA CAGCTCAGTT ACAAAGTGAA AGTGACCTGC AGGCATGCAA GCTCTGCATG
  961 TGTCAGAGGT TTTCACCGTC ATCACCGAAA CGCGCGAGAC GAAAGGGCCT CGTGATACGC
 1021 CTATTTTTAT AGGTTAATGT CATGATAATA ATGGGTTTTC TTAAGGGTCG TTTTCCATCA
 1081 CGTGCTATAA AAGTAAGTAT AGTTCAAAGT TTCAGTATAA AGTATATAAA ATTAAAAAAT
1141 TAGGCAG
//
Primer: HIS3pro_F RDB13033_A4L8b.seq
>01955_13033_A4L8_HIS3pro_F_B04_04.ab1
    1 TAGTCAATTA TACATTATAT AAAGTAATGT GATTTCTTCG AAGAATATAC TAAAAAATGA
   61 GCAGGCAAGA TAAACGAAGG CAAAGATGAC AGAGCAGAAA GCCCTAGTAA AGCGTATTAC
  121 AAATGAAACC AAGATTCAGA TTGCGATCTC TTTAAAGGGT GGTCCCCTAG CGATAGAGCA
  181 CTCGATCTTC CCAGAAAAAG AGGCAGAAGC AGTAGCAGAA CAGGCCACAC AATCGCAAGT
  241 GATTAACGTC CACACAGGTA TAGGGTTTCT GGACCATATG ATACATGCTC TGGCCAAGCA
  301 TTCCGGCTGG TCGCTAATCG TTGAGTGCAT TGGTGACTTA CACATAGACG ACCATCACAC
  361 CACTGAAGAC TGCGGGATTG CTCTCGGTCA AGCATTTAAA GAGGCCCTAG GGGCCGTGCG
  421 TGGAGTAAAA AGGTTTGGAT CAGGATTTGC GCCTTTGGAT GAGGCACTTT CCAGAGCGGT
  481 GGTAGATCTT TCGAACAGGC CGTACGCAGT TGTCGAACTT GGTTTGCAGA GGGAGAAAGT
  541 AGGAGATCTC TCTTGCGAGA TGATCCCGCA TTTTCTTGAA AGTTTTGCAG AGGCAAGCAG
  601 AATTACCCTC CACGTTGATT GTCTGCGAGG CAAGAATGAT CATCACCGTA GTGAGAGTGC
  661 GTTCAAGGCT CTTGCGGTTG CCATAAGAGA AGCCACCTCG CCCAATGGAA CCAACGATGT
  721 TCCCTCCACC AAAGGTGTTC TTATGTAGTG ACACCGATTA TTTAAAGCGG CAAGCTTACA
  781 TTTTATGTTA GCTGGTGGAC TGACGCCAGA AAATGTTGGT GATGCGCTTA GATTAAATGG
  841 CGTTATTGGT GTTGATGTAA GCGGAGGTGT GGAGACAAAT GGTGTAAAAG ACTCTAACAA
  901 AATAGCAAAT TTCGTCAAAA ATGCTAAGAA ATAGGTTATT ACTGAGTAGT ATTTATTTAA
  961 GTATTGTTTG TGCACTTGCC GGTAGAGGTG TGGTCAATAA GAGCGACCTC ATGCTATACC
 1021 TGAGAAAGCA ACCCTGACCT ACAGGAAAGA GTTACTCAAG AATAGAATTT TCGTTTTAAA
 1081 ACTAGAGTCA CTTAAATTTG GTATACACTA TTTTTTTATA ACCTTATTTA TTAAATAAAA
1141 AATCAATTAA AATCA
//
Primer: Amp_R RDB13033_A4L8c.seq
>01955_13033_A4L8_Amp_R_C04_07.ab1
    1 ATTGTTTTAT ATTATTGAGC ATTTATCAGG GTTATTGTCT CATGAGCGGA TACATATTTG
   61 AATGTATTTA GAAAAATAAA CAAATAGGGG TTCCGCGCAC ATTTCCCCGA AAAGTGCCAC
  121 CTGACGGATC GCTTGCCTGT AACTTACACG CGCCTCGTAT CTTTTAATGA TGGAATAATT
  181 TGGGAATTTA CTCTGTGTTT ATTTATTTTT ATGTTTTGTA TTTGGATTTT AGAAAGTAAA
  241 TAAAGAAGGT AGAAGAGTTA CGGAATGAAG AAAAAAAAAT AAACAAAGGT TTAAAAAATT
  301 TCAACAAAAA GCGTACTTTA CATATATATT TATTAGACAA GAAAAGCAGA TTAAATAGAT
  361 ATACATTCGA TTAACGATAA GTAAAATGTA AAATCACAGG ATTTTCGTGT GTGGTCTTCT
  421 ACACAGACAA GATGAAACAA TTCGGCATTA ATACCTGAGA GCAGGAAGAG CAAGATAAAA
  481 GGTAGTATTT GTTGGCGATC CCCCTAGAGT CTTTTACATC TTCGGAAAAC AAAAACTATT
  541 TTTTCTTTAA TTTCTTTTTT TACTTTCTAT TTTTAATTTA TATATTTATA TTAAAAAATT
  601 TAAATTATAA TTATTTTTAT AGCACGTGAT GAAAAGGACC CTAAGAAACC ATTATTATCA
  661 TGACATTAAC CTATAAAAAT AGGCGTATCA CGAGGCCCTT TCGTCTCGCG CGTTTCGGTG
  721 ATGACGGTGA AAACCTCTGA CACATGCAGA GCTTGCATGC CTGCAGGTCA CTTTCACTTT
  781 GTAACTGAGC TGTCATTTAT ATTGAATTTT CAAAAATTCT TACTTTTTTT TTGGATGGAC
  841 GCAAAGAAGT TTAATAATCA TATTACATGG CATTACCACC ATATACATAT CCATATACAT
  901 ATCCATATCT AATCTTACTT ATATGTTGTG GAAATGTAAA GAGCCCCATT ATCTTAGCCT
  961 AAAAAAAAAC CTTCTCTTTG GAAACTTTTC AGTAATACGC TTTAACTGCT CATTTGCTAT
 1021 ATTGAAAGTA CGGGATTAGA AGCCGCCCGA GCGGGTGACA GCCCCTCGGA GAGACTCTCC
 1081 TCCGTGCGTC CTTCGTCTCA ACCGATCGCG ATCTGGAAAC GCAGAATGTT GCCTTCGGCG
1141 ACCCGGTCA
//

Please visit Sequencing and PCR primers for primer information.


References

References

original Iha, H., Shuttle-vector system for Saccharomyces cerevisiae designed to produce C-terminal-Myc-tagged fusion proteins. Biotechniques, 25, 936-938 (1998). PMID 9863043.
user report Shigematsu, M., Evidence for DNA cleavage caused directly by a transfer RNA-targeting toxin. PLoS One. 8 (9): e75512 (2013). PMID 24069426.

2019.03.08

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