Please review the QC test results indicated by icon below as well as clone information before placing your order.

pGMH10 (#RDB01955)

E. coli - S. cerevisiae shuttle vector

Alternative name	E. coli-S. cerevisiae shuttle vector
Clone info.	E. coli-S. cerevisiae shuttle vector, 9E10 epitope (Myc-tag), centromere-type ori, HIS3, GAL1-10 promoter, Ampicillin
Vector backbone	pGMH10 (Plasmid)
Size of vector backbone	4,817 bp
Selectable markers	Amp^r (E. coli), HIS3 (S. cerevisiae)
Depositor|Developer	Iha, Hidekatsu |

 

Sequence	RDB01955z.seq
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Distribution information

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Ordering forms	Order form [Credit Card Payment] [Bank Transfer Payment] [Example of order form ] MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ] Please visit Information of Request for Distribution.[link] For for-profit-research purpose, please contact us.
Terms and conditions for distribution	Acknowledgement to Depositor and Developer in publication.

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Catalog #	Resource name	Shipping form	Fee (non-profit org.)
RDB01955	pGMH10	DNA solution	Please contact us.

Please review the QC test results indicated by icon below as well as clone information before placing your order.

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How to cite this biological resource

Materials & Methods section:

The pGMH10 was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB01955).

Reference section:

Iha, H., Tsurugi, K., Shuttle-vector system for Saccharomyces cerevisiae designed to produce C-terminal-Myc-tagged fusion proteins. Biotechniques, 25, 936-938 (1998). PMID 9863043. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

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QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by icon as well as clone information before placing your order.

Test sheet	RDB13033_A4L8.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: ADHrev Sequence file: RDB13033_A4L8a.seq
>01955_13033_A4L8_ADHrev_A04_01.ab1     1 GAAGGGTTCT CAAGATAAGA ATTTTCGTTT TAAAACCTAA GAGTCACTTT AAAATTTGTA    61 TACACTTATT TTTTTTATAA CTTATTTAAT AATAAAAATC ATAAATCATA AGAAATTCGC   121 CCGGAATTAG CTTGGCTGCA GGTCGACTCA CAGATCCTCT TCGGAAATCA GTTTCTGTTC   181 AGAATTCGAG CTCGGTACCC GGGGATCCTC TAGAGTCAAT TCGATCCGGG GTTTTTTCTC   241 CTTGACGTTA AAGTATAGAG GTATATTAAC AATTTTTTGT TGATACTTTT ATTACATTTG   301 AATAAGAAGT AATACAAACC GAAAATGTTG AAAGTATTAG TTAAAGTGGT TATGCAGTTT   361 TTGCATTTAT ATATCTGTTA ATAGATCAAA AATCATCGCT TCGCTGATTA ATTACCCCAG   421 AAATAAGGCT AAAAAACTAA TCGCATTATC ATCCTATGGT TGTTAATTTG ATTCGTTCAT   481 TTGAAGGTTT GTGGGGCCAG GTTACTGCCA ATTTTTCCTC TTCATAACCA TAAAAGCTAG   541 TATTGTAGAA TCTTTATTGT TCGGAGCAGT GCGGCGCGAG GCACATCTGC GTTTCAGGAA   601 CGCGACCGGT GAAGACGAGG ACGCACGGAG GAGAGTCTTC CTTCGGAGGG CTGTCACCCG   661 CTCGGCGGCT TCTAATCCGT ACTTCAATAT AGCAATGAGC AGTTAAGCGT ATTACTGAAA   721 GTTCCAAAGA GAAGGTTTTT TTAGGCTAAG ATAATGGGGC TCTTTACATT TCCACAACAT   781 ATAAGTAAGA TTAGATATGG ATATGTATAT GGATATGTAT ATGGTGGTAA TGCCATGTAA   841 TATGATTATT AAACTTCTTT GCGTCCATCC AAAAAAAAAG TAAGAATTTT TGAAAATTCA   901 ATATAAATGA CAGCTCAGTT ACAAAGTGAA AGTGACCTGC AGGCATGCAA GCTCTGCATG   961 TGTCAGAGGT TTTCACCGTC ATCACCGAAA CGCGCGAGAC GAAAGGGCCT CGTGATACGC  1021 CTATTTTTAT AGGTTAATGT CATGATAATA ATGGGTTTTC TTAAGGGTCG TTTTCCATCA  1081 CGTGCTATAA AAGTAAGTAT AGTTCAAAGT TTCAGTATAA AGTATATAAA ATTAAAAAAT 1141 TAGGCAG //
Primer: HIS3pro_F Sequence file: RDB13033_A4L8b.seq
>01955_13033_A4L8_HIS3pro_F_B04_04.ab1     1 TAGTCAATTA TACATTATAT AAAGTAATGT GATTTCTTCG AAGAATATAC TAAAAAATGA    61 GCAGGCAAGA TAAACGAAGG CAAAGATGAC AGAGCAGAAA GCCCTAGTAA AGCGTATTAC   121 AAATGAAACC AAGATTCAGA TTGCGATCTC TTTAAAGGGT GGTCCCCTAG CGATAGAGCA   181 CTCGATCTTC CCAGAAAAAG AGGCAGAAGC AGTAGCAGAA CAGGCCACAC AATCGCAAGT   241 GATTAACGTC CACACAGGTA TAGGGTTTCT GGACCATATG ATACATGCTC TGGCCAAGCA   301 TTCCGGCTGG TCGCTAATCG TTGAGTGCAT TGGTGACTTA CACATAGACG ACCATCACAC   361 CACTGAAGAC TGCGGGATTG CTCTCGGTCA AGCATTTAAA GAGGCCCTAG GGGCCGTGCG   421 TGGAGTAAAA AGGTTTGGAT CAGGATTTGC GCCTTTGGAT GAGGCACTTT CCAGAGCGGT   481 GGTAGATCTT TCGAACAGGC CGTACGCAGT TGTCGAACTT GGTTTGCAGA GGGAGAAAGT   541 AGGAGATCTC TCTTGCGAGA TGATCCCGCA TTTTCTTGAA AGTTTTGCAG AGGCAAGCAG   601 AATTACCCTC CACGTTGATT GTCTGCGAGG CAAGAATGAT CATCACCGTA GTGAGAGTGC   661 GTTCAAGGCT CTTGCGGTTG CCATAAGAGA AGCCACCTCG CCCAATGGAA CCAACGATGT   721 TCCCTCCACC AAAGGTGTTC TTATGTAGTG ACACCGATTA TTTAAAGCGG CAAGCTTACA   781 TTTTATGTTA GCTGGTGGAC TGACGCCAGA AAATGTTGGT GATGCGCTTA GATTAAATGG   841 CGTTATTGGT GTTGATGTAA GCGGAGGTGT GGAGACAAAT GGTGTAAAAG ACTCTAACAA   901 AATAGCAAAT TTCGTCAAAA ATGCTAAGAA ATAGGTTATT ACTGAGTAGT ATTTATTTAA   961 GTATTGTTTG TGCACTTGCC GGTAGAGGTG TGGTCAATAA GAGCGACCTC ATGCTATACC  1021 TGAGAAAGCA ACCCTGACCT ACAGGAAAGA GTTACTCAAG AATAGAATTT TCGTTTTAAA  1081 ACTAGAGTCA CTTAAATTTG GTATACACTA TTTTTTTATA ACCTTATTTA TTAAATAAAA 1141 AATCAATTAA AATCA //
Primer: Amp_R Sequence file: RDB13033_A4L8c.seq
>01955_13033_A4L8_Amp_R_C04_07.ab1     1 ATTGTTTTAT ATTATTGAGC ATTTATCAGG GTTATTGTCT CATGAGCGGA TACATATTTG    61 AATGTATTTA GAAAAATAAA CAAATAGGGG TTCCGCGCAC ATTTCCCCGA AAAGTGCCAC   121 CTGACGGATC GCTTGCCTGT AACTTACACG CGCCTCGTAT CTTTTAATGA TGGAATAATT   181 TGGGAATTTA CTCTGTGTTT ATTTATTTTT ATGTTTTGTA TTTGGATTTT AGAAAGTAAA   241 TAAAGAAGGT AGAAGAGTTA CGGAATGAAG AAAAAAAAAT AAACAAAGGT TTAAAAAATT   301 TCAACAAAAA GCGTACTTTA CATATATATT TATTAGACAA GAAAAGCAGA TTAAATAGAT   361 ATACATTCGA TTAACGATAA GTAAAATGTA AAATCACAGG ATTTTCGTGT GTGGTCTTCT   421 ACACAGACAA GATGAAACAA TTCGGCATTA ATACCTGAGA GCAGGAAGAG CAAGATAAAA   481 GGTAGTATTT GTTGGCGATC CCCCTAGAGT CTTTTACATC TTCGGAAAAC AAAAACTATT   541 TTTTCTTTAA TTTCTTTTTT TACTTTCTAT TTTTAATTTA TATATTTATA TTAAAAAATT   601 TAAATTATAA TTATTTTTAT AGCACGTGAT GAAAAGGACC CTAAGAAACC ATTATTATCA   661 TGACATTAAC CTATAAAAAT AGGCGTATCA CGAGGCCCTT TCGTCTCGCG CGTTTCGGTG   721 ATGACGGTGA AAACCTCTGA CACATGCAGA GCTTGCATGC CTGCAGGTCA CTTTCACTTT   781 GTAACTGAGC TGTCATTTAT ATTGAATTTT CAAAAATTCT TACTTTTTTT TTGGATGGAC   841 GCAAAGAAGT TTAATAATCA TATTACATGG CATTACCACC ATATACATAT CCATATACAT   901 ATCCATATCT AATCTTACTT ATATGTTGTG GAAATGTAAA GAGCCCCATT ATCTTAGCCT   961 AAAAAAAAAC CTTCTCTTTG GAAACTTTTC AGTAATACGC TTTAACTGCT CATTTGCTAT  1021 ATTGAAAGTA CGGGATTAGA AGCCGCCCGA GCGGGTGACA GCCCCTCGGA GAGACTCTCC  1081 TCCGTGCGTC CTTCGTCTCA ACCGATCGCG ATCTGGAAAC GCAGAATGTT GCCTTCGGCG 1141 ACCCGGTCA //

Please visit Sequencing and PCR primers for primer information.

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References

Original, user report and related articles

original	Iha, H., Shuttle-vector system for Saccharomyces cerevisiae designed to produce C-terminal-Myc-tagged fusion proteins. Biotechniques, 25, 936-938 (1998). PMID 9863043. [link to RRC of NBRP]
user_report	Shigematsu, M., Evidence for DNA cleavage caused directly by a transfer RNA-targeting toxin. PLoS One. 8 (9): e75512 (2013). PMID 24069426. [link to RRC of NBRP]

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• Expression Vector Backbone of Saccharomyces cerevisiae (English text)

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