Resource data sheet
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Please review the QC test results indicated by check icon below as well as clone information before placing your order.

pAMU10 (#RDB01953)

E. coli - S. cerevisiae shuttle vector

Alternative name E. coli-S. cerevisiae shuttle vector
Clone info. E. coli-S. cerevisiae shuttle vector, 9E10 epitope (Myc-tag), centromere-type ori, URA3, ADH1 promoter, Ampicillin
Vector backbone pAMU10 (Plasmid)
Size of vector backbone 4,575 bp
Selectable markers Amp^r (E. coli), URA3 (S. cerevisiae)
Depositor|Developer Iha, Hidekatsu |
 
Sequence RDB01953z.seq
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Distribution information

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MTAに書く使用条件 研究結果の発表時、謝辞に寄託者および樹立者名を入れること。

Catalog # Resource name Shipping form Fee (non-profit org.)
RDB01953 pAMU10 DNA solution JPY 9,460 (not-for-profit academic purpose)
plus cost of shipping containers, dry ice (if required) and shipping charge

Please review the QC test results indicated by check icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The pAMU10 was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB01953).

Reference section:

Iha, H., Tsurugi, K., Shuttle-vector system for Saccharomyces cerevisiae designed to produce C-terminal-Myc-tagged fusion proteins. Biotechniques, 25, 936-938 (1998). PMID 9863043. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB13045_A4Lj.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: ADHrev
Sequence file: RDB13045_A4Lja.seq check
>01953_13045_A4Lj_ADHrev_H09_24.ab1
    1 TCAGCTCTCA GATAGATTTT CGTTTTAAAC CTAAGAGTCA CTTTAAAATT TGTATACACT
   61 TATTTTTTTT ATAACTTATT TAATAATAAA AATCATAAAT CATAAGAAAT TCGCCCGGAA
  121 TTAGCTTGGC TGCAGGTCGA CTCACAGATC CTCTTCGGAA ATCAGTTTCT GTTCAGAATT
  181 CGAGCTCGGT ACCCGGGGAT CCTCTAGAGT CAGCTTGGAG TTGATTGTAT GCTTGGTATA
  241 GCTTGAAATA TTGTGCAGAA AAAGAAACAA GGAAGAAAGG GAACGAGAAC AATGACGAGG
  301 AAACAAAAGA TTAATAATTG CAGGTCTATT TATACTTGAT AGCAAGACAG CAAACTTTTT
  361 TTTATTTCAA ATTCAAGTAA CTGGAAGGAA GGCCGTATAC CGTTGCTCAT TAGAGAGTAG
  421 TGTGCGTGAA TGAAGGAAGG AAAAAGTTTC GTGTGCTTCG AGATACCCCT CATCAGCTCT
  481 GGAACAACGA CATCTGTTGG TGCTGTCTTT GTCGTTAATT TTTTCCTTTA GTGTCTTCCA
  541 TCATTTTTTT GTCATTGCGG ATATGGTGAG ACAACAACGG GGGAGAGAGA AAAGAAAAAA
  601 AAAGAAAAGA AGTTGCATGC AAGCTCTGCA TGTGTCAGAG GTTTTCACCG TCATCACCGA
  661 AACGCGCGAG ACGAAAGGGC CTCGTGATAC GCCTATTTTT ATAGGTTAAT GTCATGATAA
  721 TAATGGTTTC TTAGGGTCCT TTTCATCACG TGCTATAAAA ATAATTATAA TTTAAATTTT
  781 TTAATATAAA TATATAAATT AAAAATAGAA AGTAAAAAAA GAAATTAAAG AAAAAATAGT
  841 TTTTGTTTTC CGAAGATGTA AAAGACTCTA GGGGGATCGC CAACAAATAC TACCTTTTAT
  901 CTTGCTCTTC CTGCTCTCAG GTATTAATGC CGAATTGTTT CATCTTGTCT GTGTAGAAGA
  961 CCACACACGA AAATCCTGTG ATTTTACATT TTACTTATCG TTAATCGAAT GTATATCTAT
 1021 TTAATCTGCT TTTCTTGTCT AATAAAATAT ATATGTAAAG TACGCTTTTT TGATGAATTT
 1081 TTTTAAACCT TGTTAATTTT TTTTCTCATT CCGTAACTCT TCTACCCATT CCGTTTAATT
1141 TC
//
Primer: Amp_R
Sequence file: RDB13045_A4Ljb.seq check
>01953_13045_A4Lj_Amp_R_A10_01.ab1
    1 ACTTTTTTTA ATATTTATTT GAAGCATTTA TCAGGGTTAT TGTCTCATCG CAGCGGATAC
   61 ATATTTGAAT GTATTTAGAA AAATAAACAA ATAGGGGTTC CGCGCACATT TCCCCGAAAA
  121 GTGCCACCTG ACGGATCGCT TGCCTGTAAC TTACACGCGC CTCGTATCTT TTAATGATGG
  181 AATAATTTGG GAATTTACTC TGTGTTTATT TATTTTTATG TTTTGTATTT GGATTTTAGA
  241 AAGTAAATAA AGAAGGTAGA AGAGTTACGG AATGAAGAAA AAAAAATAAA CAAAGGTTTA
  301 AAAAATTTCA ACAAAAAGCG TACTTTACAT ATATATTTAT TAGACAAGAA AAGCAGATTA
  361 AATAGATATA CATTCGATTA ACGATAAGTA AAATGTAAAA TCACAGGATT TTCGTGTGTG
  421 GTCTTCTACA CAGACAAGAT GAAACAATTC GGCATTAATA CCTGAGAGCA GGAAGAGCAA
  481 GATAAAAGGT AGTATTTGTT GGCGATCCCC CTAGAGTCTT TTACATCTTC GGAAAACAAA
  541 AACTATTTTT TCTTTAATTT CTTTTTTTAC TTTCTATTTT TAATTTATAT ATTTATATTA
  601 AAAAATTTAA ATTATAATTA TTTTTATAGC ACGTGATGAA AAGGACCCTA AGAAACCATT
  661 ATTATCATGA CATTAACCTA TAAAAATAGG CGTATCACGA GGCCCTTTCG TCTCGCGCGT
  721 TTCGGTGATG ACGGTGAAAA CCTCTGACAC ATGCAGAGCT TGCATGCAAC TTCTTTTCTT
  781 TTTTTTTCTT TTCTCTCTCC CCCGTTGTTG TCTCACCATA TCCGCAATGA CAAAAAAATG
  841 ATGGAAGACA CTAAAGGAAA AAATTAACGA CAAAGACAGC ACCAACAGAT GTCGTTGTTC
  901 CAGAGCTGAT GAGGGGTATC TCGAAGCACA CGAAACTTTT TCCTTCCTTC ATTCACGCAC
  961 ACTACTCTCT AATGAGCAAC GGTATACGGC CTTCCTTCCA GTTACTTTGA AATTTGAATA
 1021 AAAAAAAAGT TTGCTGTCTT GCTATCAAGT ATAAATAGAC CTGCAATTAT AATCTTTGTT
 1081 CCTTCGTCAA TGTCTCGATT CCTTTCTTCT TGATTCCATT TTCCTGGCAC CAATAATTTT
1141 TCAAAGGC
//
Primer: URA3pro_F
Sequence file: RDB13045_A4Ljc.seq check
>01953_13045_A4Lj_URA3pro_F_B10_04.ab1
    1 CAAATTGTAG TGTTGAGACA TGAATTGCCC AGTATTCTTA ACCCAACTGC ACAGAACAAA
   61 AACCTGCAGG AAACGAAGAT AAATCATGTC GAAAGCTACA TATAAGGAAC GTGCTGCTAC
  121 TCATCCTAGT CCTGTTGCTG CCAAGCTATT TAATATCATG CACGAAAAGC AAACAAACTT
  181 GTGTGCTTCA TTGGATGTTC GTACCACCAA GGAATTACTG GAGTTAGTTG AAGCATTAGG
  241 TCCCAAAATT TGTTTACTAA AAACACATGT GGATATCTTG ACTGATTTTT CCATGGAGGG
  301 CACAGTTAAG CCGCTAAAGG CATTATCCGC CAAGTACAAT TTTTTACTCT TCGAAGACAG
  361 AAAATTTGCT GACATTGGTA ATACAGTCAA ATTGCAGTAC TCTGCGGGTG TATACAGAAT
  421 AGCAGAATGG GCAGACATTA CGAATGCACA CGGTGTGGTG GGCCCAGGTA TTGTTAGCGG
  481 TTTGAAGCAG GCGGCAGAAG AAGTAACAAA GGAACCTAGA GGCCTTTTGA TGTTAGCAGA
  541 ATTGTCATGC AAGGGCTCCC TATCTACTGG AGAATATACT AAGGGTACTG TTGACATTGC
  601 GAAGAGCGAC AAAGATTTTG TTATCGGCTT TATTGCTCAA AGAGACATGG GTGGAAGAGA
  661 TGAAGGTTAC GATTGGTTGA TTATGACACC CGGTGTGGGT TTAGATGACA AGGGAGACGC
  721 ATTGGGTCAA CAGTATAGAA CCGTGGATGA TGTGGTCTCT ACAGGATCTG ACATTATTAT
  781 TGTTGGAAGA GGACTATTTG CAAAGGGAAG GGATGCTAAG GTAGAGGGTG AACGTTACAG
  841 AAAAGCAGGC TGGGAAGCAT ATTTGAGAAG ATGCGGCCAG CAAAACTAAA AAAACTGTAT
  901 TATAAGTAAA GCTTACATTT TATGTTAGCT GGTGGACTGA CGCCAGAAAA TGTTGGTGAT
  961 GCGCTTAGAT TAAATGGCGT TATTGGGTGT TGATGTAAGC GGAGGTGTGG AGACAAATGG
 1021 TGTAAAAGAC TCTAACAAAT AGCAAATTTC GTCAAAAAAT GCTAAGAAAT AGGTTATTAC
 1081 TGAGTAGTAA TTTATTTTAG TATTTGTTTG TTGCACGTGC TGCTAGAGGT GTATGTCATA
1141 GAGGCGATCC TTCAAAGTGT GCT
//

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

original Iha, H., Shuttle-vector system for Saccharomyces cerevisiae designed to produce C-terminal-Myc-tagged fusion proteins. Biotechniques, 25, 936-938 (1998). PMID 9863043. [link to RRC of NBRP]