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pAMU10

E. coli - S. cerevisiae shuttle vector

Catalog number RDB01953
Resource name pAMU10
Alternative name E. coli-S. cerevisiae shuttle vector
Clone info. E. coli-S. cerevisiae shuttle vector, 9E10 epitope (Myc-tag), centromere-type ori, URA3, ADH1 promoter, Ampicillin
Vector backbone pAMU10 (Plasmid)
Size of vector backbone 4,575 bp
Selectable markers Amp^r (E. coli), URA3 (S. cerevisiae)
Depositor|Developer Iha, Hidekatsu |
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External Database

            Reference sequence
              

            Distribution information

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            RDB01953 pAMU10 DNA solution

            Ordering Information [in Japanese] [in English]
            Please review the QC test results indicated by check icon below as well as clone information before placing your order.

            References and tips

            Electronic file

            Sequence RDB01953z.seq
            Remarks, protocol and/or map (gif) RDB01953.gif
            Remarks, protocol and/or map (gif) RDB01953z.gif

            Original reference

            original Iha, H., Shuttle-vector system for Saccharomyces cerevisiae designed to produce C-terminal-Myc-tagged fusion proteins. Biotechniques, 25, 936-938 (1998). PMID 9863043.

            Further references such as user reports and related articles (go to bottom)

            Featured content

            Featured content Expression Vector Backbone of Saccharomyces cerevisiae (English text)

            Sequence information

            RIKEN BRC has sequenced portions of this material for quality test.
            Please review the QC test results indicated by check icon as well as clone information before placing your order.

            Test sheet RDB13045_A4Lj.pdf check

            Nucleotide sequence of a portion of this resource (if available).

            Primer: ADHrev
            Sequence file: RDB13045_A4Lja.seq check
            >01953_13045_A4Lj_ADHrev_H09_24.ab1
                1 TCAGCTCTCA GATAGATTTT CGTTTTAAAC CTAAGAGTCA CTTTAAAATT TGTATACACT
               61 TATTTTTTTT ATAACTTATT TAATAATAAA AATCATAAAT CATAAGAAAT TCGCCCGGAA
              121 TTAGCTTGGC TGCAGGTCGA CTCACAGATC CTCTTCGGAA ATCAGTTTCT GTTCAGAATT
              181 CGAGCTCGGT ACCCGGGGAT CCTCTAGAGT CAGCTTGGAG TTGATTGTAT GCTTGGTATA
              241 GCTTGAAATA TTGTGCAGAA AAAGAAACAA GGAAGAAAGG GAACGAGAAC AATGACGAGG
              301 AAACAAAAGA TTAATAATTG CAGGTCTATT TATACTTGAT AGCAAGACAG CAAACTTTTT
              361 TTTATTTCAA ATTCAAGTAA CTGGAAGGAA GGCCGTATAC CGTTGCTCAT TAGAGAGTAG
              421 TGTGCGTGAA TGAAGGAAGG AAAAAGTTTC GTGTGCTTCG AGATACCCCT CATCAGCTCT
              481 GGAACAACGA CATCTGTTGG TGCTGTCTTT GTCGTTAATT TTTTCCTTTA GTGTCTTCCA
              541 TCATTTTTTT GTCATTGCGG ATATGGTGAG ACAACAACGG GGGAGAGAGA AAAGAAAAAA
              601 AAAGAAAAGA AGTTGCATGC AAGCTCTGCA TGTGTCAGAG GTTTTCACCG TCATCACCGA
              661 AACGCGCGAG ACGAAAGGGC CTCGTGATAC GCCTATTTTT ATAGGTTAAT GTCATGATAA
              721 TAATGGTTTC TTAGGGTCCT TTTCATCACG TGCTATAAAA ATAATTATAA TTTAAATTTT
              781 TTAATATAAA TATATAAATT AAAAATAGAA AGTAAAAAAA GAAATTAAAG AAAAAATAGT
              841 TTTTGTTTTC CGAAGATGTA AAAGACTCTA GGGGGATCGC CAACAAATAC TACCTTTTAT
              901 CTTGCTCTTC CTGCTCTCAG GTATTAATGC CGAATTGTTT CATCTTGTCT GTGTAGAAGA
              961 CCACACACGA AAATCCTGTG ATTTTACATT TTACTTATCG TTAATCGAAT GTATATCTAT
             1021 TTAATCTGCT TTTCTTGTCT AATAAAATAT ATATGTAAAG TACGCTTTTT TGATGAATTT
             1081 TTTTAAACCT TGTTAATTTT TTTTCTCATT CCGTAACTCT TCTACCCATT CCGTTTAATT
            1141 TC
            //
            Primer: Amp_R
            Sequence file: RDB13045_A4Ljb.seq check
            >01953_13045_A4Lj_Amp_R_A10_01.ab1
                1 ACTTTTTTTA ATATTTATTT GAAGCATTTA TCAGGGTTAT TGTCTCATCG CAGCGGATAC
               61 ATATTTGAAT GTATTTAGAA AAATAAACAA ATAGGGGTTC CGCGCACATT TCCCCGAAAA
              121 GTGCCACCTG ACGGATCGCT TGCCTGTAAC TTACACGCGC CTCGTATCTT TTAATGATGG
              181 AATAATTTGG GAATTTACTC TGTGTTTATT TATTTTTATG TTTTGTATTT GGATTTTAGA
              241 AAGTAAATAA AGAAGGTAGA AGAGTTACGG AATGAAGAAA AAAAAATAAA CAAAGGTTTA
              301 AAAAATTTCA ACAAAAAGCG TACTTTACAT ATATATTTAT TAGACAAGAA AAGCAGATTA
              361 AATAGATATA CATTCGATTA ACGATAAGTA AAATGTAAAA TCACAGGATT TTCGTGTGTG
              421 GTCTTCTACA CAGACAAGAT GAAACAATTC GGCATTAATA CCTGAGAGCA GGAAGAGCAA
              481 GATAAAAGGT AGTATTTGTT GGCGATCCCC CTAGAGTCTT TTACATCTTC GGAAAACAAA
              541 AACTATTTTT TCTTTAATTT CTTTTTTTAC TTTCTATTTT TAATTTATAT ATTTATATTA
              601 AAAAATTTAA ATTATAATTA TTTTTATAGC ACGTGATGAA AAGGACCCTA AGAAACCATT
              661 ATTATCATGA CATTAACCTA TAAAAATAGG CGTATCACGA GGCCCTTTCG TCTCGCGCGT
              721 TTCGGTGATG ACGGTGAAAA CCTCTGACAC ATGCAGAGCT TGCATGCAAC TTCTTTTCTT
              781 TTTTTTTCTT TTCTCTCTCC CCCGTTGTTG TCTCACCATA TCCGCAATGA CAAAAAAATG
              841 ATGGAAGACA CTAAAGGAAA AAATTAACGA CAAAGACAGC ACCAACAGAT GTCGTTGTTC
              901 CAGAGCTGAT GAGGGGTATC TCGAAGCACA CGAAACTTTT TCCTTCCTTC ATTCACGCAC
              961 ACTACTCTCT AATGAGCAAC GGTATACGGC CTTCCTTCCA GTTACTTTGA AATTTGAATA
             1021 AAAAAAAAGT TTGCTGTCTT GCTATCAAGT ATAAATAGAC CTGCAATTAT AATCTTTGTT
             1081 CCTTCGTCAA TGTCTCGATT CCTTTCTTCT TGATTCCATT TTCCTGGCAC CAATAATTTT
            1141 TCAAAGGC
            //
            Primer: URA3pro_F
            Sequence file: RDB13045_A4Ljc.seq check
            >01953_13045_A4Lj_URA3pro_F_B10_04.ab1
                1 CAAATTGTAG TGTTGAGACA TGAATTGCCC AGTATTCTTA ACCCAACTGC ACAGAACAAA
               61 AACCTGCAGG AAACGAAGAT AAATCATGTC GAAAGCTACA TATAAGGAAC GTGCTGCTAC
              121 TCATCCTAGT CCTGTTGCTG CCAAGCTATT TAATATCATG CACGAAAAGC AAACAAACTT
              181 GTGTGCTTCA TTGGATGTTC GTACCACCAA GGAATTACTG GAGTTAGTTG AAGCATTAGG
              241 TCCCAAAATT TGTTTACTAA AAACACATGT GGATATCTTG ACTGATTTTT CCATGGAGGG
              301 CACAGTTAAG CCGCTAAAGG CATTATCCGC CAAGTACAAT TTTTTACTCT TCGAAGACAG
              361 AAAATTTGCT GACATTGGTA ATACAGTCAA ATTGCAGTAC TCTGCGGGTG TATACAGAAT
              421 AGCAGAATGG GCAGACATTA CGAATGCACA CGGTGTGGTG GGCCCAGGTA TTGTTAGCGG
              481 TTTGAAGCAG GCGGCAGAAG AAGTAACAAA GGAACCTAGA GGCCTTTTGA TGTTAGCAGA
              541 ATTGTCATGC AAGGGCTCCC TATCTACTGG AGAATATACT AAGGGTACTG TTGACATTGC
              601 GAAGAGCGAC AAAGATTTTG TTATCGGCTT TATTGCTCAA AGAGACATGG GTGGAAGAGA
              661 TGAAGGTTAC GATTGGTTGA TTATGACACC CGGTGTGGGT TTAGATGACA AGGGAGACGC
              721 ATTGGGTCAA CAGTATAGAA CCGTGGATGA TGTGGTCTCT ACAGGATCTG ACATTATTAT
              781 TGTTGGAAGA GGACTATTTG CAAAGGGAAG GGATGCTAAG GTAGAGGGTG AACGTTACAG
              841 AAAAGCAGGC TGGGAAGCAT ATTTGAGAAG ATGCGGCCAG CAAAACTAAA AAAACTGTAT
              901 TATAAGTAAA GCTTACATTT TATGTTAGCT GGTGGACTGA CGCCAGAAAA TGTTGGTGAT
              961 GCGCTTAGAT TAAATGGCGT TATTGGGTGT TGATGTAAGC GGAGGTGTGG AGACAAATGG
             1021 TGTAAAAGAC TCTAACAAAT AGCAAATTTC GTCAAAAAAT GCTAAGAAAT AGGTTATTAC
             1081 TGAGTAGTAA TTTATTTTAG TATTTGTTTG TTGCACGTGC TGCTAGAGGT GTATGTCATA
            1141 GAGGCGATCC TTCAAAGTGT GCT
            //

            Please visit Sequencing and PCR primers for primer information.


            References

            Original, user report and related articles

            original Iha, H., Shuttle-vector system for Saccharomyces cerevisiae designed to produce C-terminal-Myc-tagged fusion proteins. Biotechniques, 25, 936-938 (1998). PMID 9863043.

            2022.02.09

            GNP_filter3_RDBDEP_html_220112.pl