RDB01953 - pAMU10

Resource data sheet
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pAMU10

E. coli - S. cerevisiae shuttle vector

Catalog number	RDB01953
Resource name	pAMU10
Alternative name	E. coli-S. cerevisiae shuttle vector
Clone info.	E. coli-S. cerevisiae shuttle vector, 9E10 epitope (Myc-tag), centromere-type ori, URA3, ADH1 promoter, Ampicillin
Vector backbone	pAMU10 (Plasmid)
Size of vector backbone	4,575 bp
Selectable markers	Amp^r (E. coli), URA3 (S. cerevisiae)
Depositor	Iha, Hidekatsu \|

Distribution information

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Catalog #	Resource name	Shipping form	Fee (non-profit org.)
RDB01953	pAMU10	DNA solution

Ordering Information [in Japanese] [in English]

References and tips

Electronic file

Electronic file	Sequence RDB01953z.seq from Depositor
Electronic file	Map RDB01953.gif from Depositor
Electronic file	Map RDB01953z.gif from Depositor

Featured content	Expression Vector Backbone of Saccharomyces cerevisiae (English text)

Sequence information

RIKEN BRC has sequenced portions of this material for quality test. Primers and the results are shown below.

Data are summerized on test sheet below.

Test sheet	RDB13045_A4Lj.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: ADHrev	RDB13045_A4Lja.seq
>01953_13045_A4Lj_ADHrev_H09_24.ab1 1 TCAGCTCTCA GATAGATTTT CGTTTTAAAC CTAAGAGTCA CTTTAAAATT TGTATACACT 61 TATTTTTTTT ATAACTTATT TAATAATAAA AATCATAAAT CATAAGAAAT TCGCCCGGAA 121 TTAGCTTGGC TGCAGGTCGA CTCACAGATC CTCTTCGGAA ATCAGTTTCT GTTCAGAATT 181 CGAGCTCGGT ACCCGGGGAT CCTCTAGAGT CAGCTTGGAG TTGATTGTAT GCTTGGTATA 241 GCTTGAAATA TTGTGCAGAA AAAGAAACAA GGAAGAAAGG GAACGAGAAC AATGACGAGG 301 AAACAAAAGA TTAATAATTG CAGGTCTATT TATACTTGAT AGCAAGACAG CAAACTTTTT 361 TTTATTTCAA ATTCAAGTAA CTGGAAGGAA GGCCGTATAC CGTTGCTCAT TAGAGAGTAG 421 TGTGCGTGAA TGAAGGAAGG AAAAAGTTTC GTGTGCTTCG AGATACCCCT CATCAGCTCT 481 GGAACAACGA CATCTGTTGG TGCTGTCTTT GTCGTTAATT TTTTCCTTTA GTGTCTTCCA 541 TCATTTTTTT GTCATTGCGG ATATGGTGAG ACAACAACGG GGGAGAGAGA AAAGAAAAAA 601 AAAGAAAAGA AGTTGCATGC AAGCTCTGCA TGTGTCAGAG GTTTTCACCG TCATCACCGA 661 AACGCGCGAG ACGAAAGGGC CTCGTGATAC GCCTATTTTT ATAGGTTAAT GTCATGATAA 721 TAATGGTTTC TTAGGGTCCT TTTCATCACG TGCTATAAAA ATAATTATAA TTTAAATTTT 781 TTAATATAAA TATATAAATT AAAAATAGAA AGTAAAAAAA GAAATTAAAG AAAAAATAGT 841 TTTTGTTTTC CGAAGATGTA AAAGACTCTA GGGGGATCGC CAACAAATAC TACCTTTTAT 901 CTTGCTCTTC CTGCTCTCAG GTATTAATGC CGAATTGTTT CATCTTGTCT GTGTAGAAGA 961 CCACACACGA AAATCCTGTG ATTTTACATT TTACTTATCG TTAATCGAAT GTATATCTAT 1021 TTAATCTGCT TTTCTTGTCT AATAAAATAT ATATGTAAAG TACGCTTTTT TGATGAATTT 1081 TTTTAAACCT TGTTAATTTT TTTTCTCATT CCGTAACTCT TCTACCCATT CCGTTTAATT 1141 TC //
Primer: Amp_R	RDB13045_A4Ljb.seq
>01953_13045_A4Lj_Amp_R_A10_01.ab1 1 ACTTTTTTTA ATATTTATTT GAAGCATTTA TCAGGGTTAT TGTCTCATCG CAGCGGATAC 61 ATATTTGAAT GTATTTAGAA AAATAAACAA ATAGGGGTTC CGCGCACATT TCCCCGAAAA 121 GTGCCACCTG ACGGATCGCT TGCCTGTAAC TTACACGCGC CTCGTATCTT TTAATGATGG 181 AATAATTTGG GAATTTACTC TGTGTTTATT TATTTTTATG TTTTGTATTT GGATTTTAGA 241 AAGTAAATAA AGAAGGTAGA AGAGTTACGG AATGAAGAAA AAAAAATAAA CAAAGGTTTA 301 AAAAATTTCA ACAAAAAGCG TACTTTACAT ATATATTTAT TAGACAAGAA AAGCAGATTA 361 AATAGATATA CATTCGATTA ACGATAAGTA AAATGTAAAA TCACAGGATT TTCGTGTGTG 421 GTCTTCTACA CAGACAAGAT GAAACAATTC GGCATTAATA CCTGAGAGCA GGAAGAGCAA 481 GATAAAAGGT AGTATTTGTT GGCGATCCCC CTAGAGTCTT TTACATCTTC GGAAAACAAA 541 AACTATTTTT TCTTTAATTT CTTTTTTTAC TTTCTATTTT TAATTTATAT ATTTATATTA 601 AAAAATTTAA ATTATAATTA TTTTTATAGC ACGTGATGAA AAGGACCCTA AGAAACCATT 661 ATTATCATGA CATTAACCTA TAAAAATAGG CGTATCACGA GGCCCTTTCG TCTCGCGCGT 721 TTCGGTGATG ACGGTGAAAA CCTCTGACAC ATGCAGAGCT TGCATGCAAC TTCTTTTCTT 781 TTTTTTTCTT TTCTCTCTCC CCCGTTGTTG TCTCACCATA TCCGCAATGA CAAAAAAATG 841 ATGGAAGACA CTAAAGGAAA AAATTAACGA CAAAGACAGC ACCAACAGAT GTCGTTGTTC 901 CAGAGCTGAT GAGGGGTATC TCGAAGCACA CGAAACTTTT TCCTTCCTTC ATTCACGCAC 961 ACTACTCTCT AATGAGCAAC GGTATACGGC CTTCCTTCCA GTTACTTTGA AATTTGAATA 1021 AAAAAAAAGT TTGCTGTCTT GCTATCAAGT ATAAATAGAC CTGCAATTAT AATCTTTGTT 1081 CCTTCGTCAA TGTCTCGATT CCTTTCTTCT TGATTCCATT TTCCTGGCAC CAATAATTTT 1141 TCAAAGGC //
Primer: URA3pro_F	RDB13045_A4Ljc.seq
>01953_13045_A4Lj_URA3pro_F_B10_04.ab1 1 CAAATTGTAG TGTTGAGACA TGAATTGCCC AGTATTCTTA ACCCAACTGC ACAGAACAAA 61 AACCTGCAGG AAACGAAGAT AAATCATGTC GAAAGCTACA TATAAGGAAC GTGCTGCTAC 121 TCATCCTAGT CCTGTTGCTG CCAAGCTATT TAATATCATG CACGAAAAGC AAACAAACTT 181 GTGTGCTTCA TTGGATGTTC GTACCACCAA GGAATTACTG GAGTTAGTTG AAGCATTAGG 241 TCCCAAAATT TGTTTACTAA AAACACATGT GGATATCTTG ACTGATTTTT CCATGGAGGG 301 CACAGTTAAG CCGCTAAAGG CATTATCCGC CAAGTACAAT TTTTTACTCT TCGAAGACAG 361 AAAATTTGCT GACATTGGTA ATACAGTCAA ATTGCAGTAC TCTGCGGGTG TATACAGAAT 421 AGCAGAATGG GCAGACATTA CGAATGCACA CGGTGTGGTG GGCCCAGGTA TTGTTAGCGG 481 TTTGAAGCAG GCGGCAGAAG AAGTAACAAA GGAACCTAGA GGCCTTTTGA TGTTAGCAGA 541 ATTGTCATGC AAGGGCTCCC TATCTACTGG AGAATATACT AAGGGTACTG TTGACATTGC 601 GAAGAGCGAC AAAGATTTTG TTATCGGCTT TATTGCTCAA AGAGACATGG GTGGAAGAGA 661 TGAAGGTTAC GATTGGTTGA TTATGACACC CGGTGTGGGT TTAGATGACA AGGGAGACGC 721 ATTGGGTCAA CAGTATAGAA CCGTGGATGA TGTGGTCTCT ACAGGATCTG ACATTATTAT 781 TGTTGGAAGA GGACTATTTG CAAAGGGAAG GGATGCTAAG GTAGAGGGTG AACGTTACAG 841 AAAAGCAGGC TGGGAAGCAT ATTTGAGAAG ATGCGGCCAG CAAAACTAAA AAAACTGTAT 901 TATAAGTAAA GCTTACATTT TATGTTAGCT GGTGGACTGA CGCCAGAAAA TGTTGGTGAT 961 GCGCTTAGAT TAAATGGCGT TATTGGGTGT TGATGTAAGC GGAGGTGTGG AGACAAATGG 1021 TGTAAAAGAC TCTAACAAAT AGCAAATTTC GTCAAAAAAT GCTAAGAAAT AGGTTATTAC 1081 TGAGTAGTAA TTTATTTTAG TATTTGTTTG TTGCACGTGC TGCTAGAGGT GTATGTCATA 1141 GAGGCGATCC TTCAAAGTGT GCT //

Please visit Sequencing and PCR primers for primer information.

References

original	Iha, H., Shuttle-vector system for Saccharomyces cerevisiae designed to produce C-terminal-Myc-tagged fusion proteins. Biotechniques, 25, 936-938 (1998). PMID 9863043.

2018.12.11

GNP_filter3_RDBDEP_html_181012.pl