Please review the QC test results indicated by icon below as well as clone information before placing your order.

pAMU10

E. coli - S. cerevisiae shuttle vector

Distribution information

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Please review the QC test results indicated by icon below as well as clone information before placing your order.

References and tips

Electronic file

Sequence	RDB01953z.seq
Remarks, protocol and/or map (gif)	RDB01953.gif
Remarks, protocol and/or map (gif)	RDB01953z.gif

Original reference

Further references such as user reports and related articles (go to bottom)

Featured content

Sequence information

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by icon as well as clone information before placing your order.

Test sheet	RDB13045_A4Lj.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: ADHrev Sequence file: RDB13045_A4Lja.seq
>01953_13045_A4Lj_ADHrev_H09_24.ab1     1 TCAGCTCTCA GATAGATTTT CGTTTTAAAC CTAAGAGTCA CTTTAAAATT TGTATACACT    61 TATTTTTTTT ATAACTTATT TAATAATAAA AATCATAAAT CATAAGAAAT TCGCCCGGAA   121 TTAGCTTGGC TGCAGGTCGA CTCACAGATC CTCTTCGGAA ATCAGTTTCT GTTCAGAATT   181 CGAGCTCGGT ACCCGGGGAT CCTCTAGAGT CAGCTTGGAG TTGATTGTAT GCTTGGTATA   241 GCTTGAAATA TTGTGCAGAA AAAGAAACAA GGAAGAAAGG GAACGAGAAC AATGACGAGG   301 AAACAAAAGA TTAATAATTG CAGGTCTATT TATACTTGAT AGCAAGACAG CAAACTTTTT   361 TTTATTTCAA ATTCAAGTAA CTGGAAGGAA GGCCGTATAC CGTTGCTCAT TAGAGAGTAG   421 TGTGCGTGAA TGAAGGAAGG AAAAAGTTTC GTGTGCTTCG AGATACCCCT CATCAGCTCT   481 GGAACAACGA CATCTGTTGG TGCTGTCTTT GTCGTTAATT TTTTCCTTTA GTGTCTTCCA   541 TCATTTTTTT GTCATTGCGG ATATGGTGAG ACAACAACGG GGGAGAGAGA AAAGAAAAAA   601 AAAGAAAAGA AGTTGCATGC AAGCTCTGCA TGTGTCAGAG GTTTTCACCG TCATCACCGA   661 AACGCGCGAG ACGAAAGGGC CTCGTGATAC GCCTATTTTT ATAGGTTAAT GTCATGATAA   721 TAATGGTTTC TTAGGGTCCT TTTCATCACG TGCTATAAAA ATAATTATAA TTTAAATTTT   781 TTAATATAAA TATATAAATT AAAAATAGAA AGTAAAAAAA GAAATTAAAG AAAAAATAGT   841 TTTTGTTTTC CGAAGATGTA AAAGACTCTA GGGGGATCGC CAACAAATAC TACCTTTTAT   901 CTTGCTCTTC CTGCTCTCAG GTATTAATGC CGAATTGTTT CATCTTGTCT GTGTAGAAGA   961 CCACACACGA AAATCCTGTG ATTTTACATT TTACTTATCG TTAATCGAAT GTATATCTAT  1021 TTAATCTGCT TTTCTTGTCT AATAAAATAT ATATGTAAAG TACGCTTTTT TGATGAATTT  1081 TTTTAAACCT TGTTAATTTT TTTTCTCATT CCGTAACTCT TCTACCCATT CCGTTTAATT 1141 TC //
Primer: Amp_R Sequence file: RDB13045_A4Ljb.seq
>01953_13045_A4Lj_Amp_R_A10_01.ab1     1 ACTTTTTTTA ATATTTATTT GAAGCATTTA TCAGGGTTAT TGTCTCATCG CAGCGGATAC    61 ATATTTGAAT GTATTTAGAA AAATAAACAA ATAGGGGTTC CGCGCACATT TCCCCGAAAA   121 GTGCCACCTG ACGGATCGCT TGCCTGTAAC TTACACGCGC CTCGTATCTT TTAATGATGG   181 AATAATTTGG GAATTTACTC TGTGTTTATT TATTTTTATG TTTTGTATTT GGATTTTAGA   241 AAGTAAATAA AGAAGGTAGA AGAGTTACGG AATGAAGAAA AAAAAATAAA CAAAGGTTTA   301 AAAAATTTCA ACAAAAAGCG TACTTTACAT ATATATTTAT TAGACAAGAA AAGCAGATTA   361 AATAGATATA CATTCGATTA ACGATAAGTA AAATGTAAAA TCACAGGATT TTCGTGTGTG   421 GTCTTCTACA CAGACAAGAT GAAACAATTC GGCATTAATA CCTGAGAGCA GGAAGAGCAA   481 GATAAAAGGT AGTATTTGTT GGCGATCCCC CTAGAGTCTT TTACATCTTC GGAAAACAAA   541 AACTATTTTT TCTTTAATTT CTTTTTTTAC TTTCTATTTT TAATTTATAT ATTTATATTA   601 AAAAATTTAA ATTATAATTA TTTTTATAGC ACGTGATGAA AAGGACCCTA AGAAACCATT   661 ATTATCATGA CATTAACCTA TAAAAATAGG CGTATCACGA GGCCCTTTCG TCTCGCGCGT   721 TTCGGTGATG ACGGTGAAAA CCTCTGACAC ATGCAGAGCT TGCATGCAAC TTCTTTTCTT   781 TTTTTTTCTT TTCTCTCTCC CCCGTTGTTG TCTCACCATA TCCGCAATGA CAAAAAAATG   841 ATGGAAGACA CTAAAGGAAA AAATTAACGA CAAAGACAGC ACCAACAGAT GTCGTTGTTC   901 CAGAGCTGAT GAGGGGTATC TCGAAGCACA CGAAACTTTT TCCTTCCTTC ATTCACGCAC   961 ACTACTCTCT AATGAGCAAC GGTATACGGC CTTCCTTCCA GTTACTTTGA AATTTGAATA  1021 AAAAAAAAGT TTGCTGTCTT GCTATCAAGT ATAAATAGAC CTGCAATTAT AATCTTTGTT  1081 CCTTCGTCAA TGTCTCGATT CCTTTCTTCT TGATTCCATT TTCCTGGCAC CAATAATTTT 1141 TCAAAGGC //
Primer: URA3pro_F Sequence file: RDB13045_A4Ljc.seq
>01953_13045_A4Lj_URA3pro_F_B10_04.ab1     1 CAAATTGTAG TGTTGAGACA TGAATTGCCC AGTATTCTTA ACCCAACTGC ACAGAACAAA    61 AACCTGCAGG AAACGAAGAT AAATCATGTC GAAAGCTACA TATAAGGAAC GTGCTGCTAC   121 TCATCCTAGT CCTGTTGCTG CCAAGCTATT TAATATCATG CACGAAAAGC AAACAAACTT   181 GTGTGCTTCA TTGGATGTTC GTACCACCAA GGAATTACTG GAGTTAGTTG AAGCATTAGG   241 TCCCAAAATT TGTTTACTAA AAACACATGT GGATATCTTG ACTGATTTTT CCATGGAGGG   301 CACAGTTAAG CCGCTAAAGG CATTATCCGC CAAGTACAAT TTTTTACTCT TCGAAGACAG   361 AAAATTTGCT GACATTGGTA ATACAGTCAA ATTGCAGTAC TCTGCGGGTG TATACAGAAT   421 AGCAGAATGG GCAGACATTA CGAATGCACA CGGTGTGGTG GGCCCAGGTA TTGTTAGCGG   481 TTTGAAGCAG GCGGCAGAAG AAGTAACAAA GGAACCTAGA GGCCTTTTGA TGTTAGCAGA   541 ATTGTCATGC AAGGGCTCCC TATCTACTGG AGAATATACT AAGGGTACTG TTGACATTGC   601 GAAGAGCGAC AAAGATTTTG TTATCGGCTT TATTGCTCAA AGAGACATGG GTGGAAGAGA   661 TGAAGGTTAC GATTGGTTGA TTATGACACC CGGTGTGGGT TTAGATGACA AGGGAGACGC   721 ATTGGGTCAA CAGTATAGAA CCGTGGATGA TGTGGTCTCT ACAGGATCTG ACATTATTAT   781 TGTTGGAAGA GGACTATTTG CAAAGGGAAG GGATGCTAAG GTAGAGGGTG AACGTTACAG   841 AAAAGCAGGC TGGGAAGCAT ATTTGAGAAG ATGCGGCCAG CAAAACTAAA AAAACTGTAT   901 TATAAGTAAA GCTTACATTT TATGTTAGCT GGTGGACTGA CGCCAGAAAA TGTTGGTGAT   961 GCGCTTAGAT TAAATGGCGT TATTGGGTGT TGATGTAAGC GGAGGTGTGG AGACAAATGG  1021 TGTAAAAGAC TCTAACAAAT AGCAAATTTC GTCAAAAAAT GCTAAGAAAT AGGTTATTAC  1081 TGAGTAGTAA TTTATTTTAG TATTTGTTTG TTGCACGTGC TGCTAGAGGT GTATGTCATA 1141 GAGGCGATCC TTCAAAGTGT GCT //

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

2022.02.09

GNP_filter3_RDBDEP_html_220112.pl