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pAMT10

E. coli - S. cerevisiae shuttle vector

Catalog number RDB01951
Resource name pAMT10
Alternative name E. coli-S. cerevisiae shuttle vector
Clone info. E. coli-S. cerevisiae shuttle vector, 9E10 epitope (Myc-tag), centromere-type ori, TRP1, ADH1 promoter, Ampicillin
Vector backbone pAMT10 (Plasmid)
Size of vector backbone 4,319 bp
Selectable markers Amp^r (E. coli), TRP1 (S. cerevisiae)
Depositor Iha, Hidekatsu |

Distribution information

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RDB01951 pAMT10 DNA solution

Ordering Information [in Japanese] [in English]

References and tips

Electronic file

Electronic file Sequence RDB01951z.seq from Depositor
Electronic file Map RDB01951z.gif from Depositor
Electronic file Map RDB01951.gif from Depositor

Featured content

Featured content Expression Vector Backbone of Saccharomyces cerevisiae (English text)

References

original Iha, H., Shuttle-vector system for Saccharomyces cerevisiae designed to produce C-terminal-Myc-tagged fusion proteins. Biotechniques, 25, 936-938 (1998). PMID 9863043.

User reports and related articles (go to bottom)


Sequence information

RIKEN BRC has sequenced portions of this material for quality test. Primers and the results are shown below.

Data are summerized on test sheet below.

Test sheet RDB13044_A4Lj.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: ADHrev RDB13044_A4Lja.seq
>01951_13044_A4Lj_ADHrev_E09_15.ab1
    1 AGAAGCTATC AGATAGATTT TCGTTTTAAA CCTAAGAGTC ACTTTAAAAT TTGTATACAC
   61 TTATTTTTTT TATAACTTAT TTAATAATAA AAATCATAAA TCATAAGAAA TTCGCCCGGA
  121 ATTAGCTTGG CTGCAGGTCG ACTCACAGAT CCTCTTCGGA AATCAGTTTC TGTTCAGAAT
  181 TCGAGCTCGG TACCCGGGGA TCCTCTAGAG TCAGCTTGGA GTTGATTGTA TGCTTGGTAT
  241 AGCTTGAAAT ATTGTGCAGA AAAAGAAACA AGGAAGAAAG GGAACGAGAA CAATGACGAG
  301 GAAACAAAAG ATTAATAATT GCAGGTCTAT TTATACTTGA TAGCAAGACA GCAAACTTTT
  361 TTTTATTTCA AATTCAAGTA ACTGGAAGGA AGGCCGTATA CCGTTGCTCA TTAGAGAGTA
  421 GTGTGCGTGA ATGAAGGAAG GAAAAAGTTT CGTGTGCTTC GAGATACCCC TCATCAGCTC
  481 TGGAACAACG ACATCTGTTG GTGCTGTCTT TGTCGTTAAT TTTTTCCTTT AGTGTCTTCC
  541 ATCATTTTTT TGTCATTGCG GATATGGTGA GACAACAACG GGGGAGAGAG AAAAGAAAAA
  601 AAAAGAAAAG AAGTTGCATG CAAGCTCTGC ATGTGTCAGA GGTTTTCACC GTCATCACCG
  661 AAACGCGCGA GACGAAAGGG CCTCGTGATA CGCCTATTTT TATAGGTTAA TGTCATGATA
  721 ATAATGGTTT CTTAGGGTCC TTTTCATCAC GTGCTATAAA AATAATTATA ATTTAAATTT
  781 TTTAATATAA ATATATAAAT TAAAAATAGA AAGTAAAAAA AGAAATTAAA GAAAAAATAG
  841 TTTTTGTTTT CCGAAGATGT AAAAGACTCT AGGGGGATCG CCAACAAATA CTACCTTTTA
  901 TCTTGCTCTT CCTGCTCTCA GGTATTAATG CCGAATTGTT TCATCTTGTC TGTGTAGAAG
  961 ACCACACACG AAAATCCTGT GATTTTACAT TTTACTTTAT CGTTAATCGA ATGTATATCT
 1021 ATTTAATCTG CTTTTTCTTG TCTAATAAAA TATATATGTA AGTACGCTTT TTGATGGAAG
 1081 TTTTTAAACG TTTGATTATT TTTTTTCTTC ATCGGTAACT TCTTTCTAAC ATTCGTTTAG
1141 TTTAACCTTT CTCTGGA
//
Primer: Amp_R RDB13044_A4Ljb.seq
>01951_13044_A4Lj_Amp_R_F09_18.ab1
    1 TTCGTTTTCA TATTATTGAG CATTTATCAG GGTTATTGTC TCATGAGCGG ATACATATTT
   61 GAATGTATTT AGAAAAATAA ACAAATAGGG GTTCCGCGCA CATTTCCCCG AAAAGTGCCA
  121 CCTGACGGAT CGCTTGCCTG TAACTTACAC GCGCCTCGTA TCTTTTAATG ATGGAATAAT
  181 TTGGGAATTT ACTCTGTGTT TATTTATTTT TATGTTTTGT ATTTGGATTT TAGAAAGTAA
  241 ATAAAGAAGG TAGAAGAGTT ACGGAATGAA GAAAAAAAAA TAAACAAAGG TTTAAAAAAT
  301 TTCAACAAAA AGCGTACTTT ACATATATAT TTATTAGACA AGAAAAGCAG ATTAAATAGA
  361 TATACATTCG ATTAACGATA AGTAAAATGT AAAATCACAG GATTTTCGTG TGTGGTCTTC
  421 TACACAGACA AGATGAAACA ATTCGGCATT AATACCTGAG AGCAGGAAGA GCAAGATAAA
  481 AGGTAGTATT TGTTGGCGAT CCCCCTAGAG TCTTTTACAT CTTCGGAAAA CAAAAACTAT
  541 TTTTTCTTTA ATTTCTTTTT TTACTTTCTA TTTTTAATTT ATATATTTAT ATTAAAAAAT
  601 TTAAATTATA ATTATTTTTA TAGCACGTGA TGAAAAGGAC CCTAAGAAAC CATTATTATC
  661 ATGACATTAA CCTATAAAAA TAGGCGTATC ACGAGGCCCT TTCGTCTCGC GCGTTTCGGT
  721 GATGACGGTG AAAACCTCTG ACACATGCAG AGCTTGCATG CAACTTCTTT TCTTTTTTTT
  781 TCTTTTCTCT CTCCCCCGTT GTTGTCTCAC CATATCCGCA ATGACAAAAA AATGATGGAA
  841 GACACTAAAG GAAAAAATTA ACGACAAAGA CAGCACCAAC AGATGTCGTT GTTCCAGAGC
  901 TGATGAGGGG TATCTCGAAG CACACGAAAC TTTTTCCTTC CTTCATTCAC GCACACTACT
  961 CTCTAATGAG CAACGGTATA CGGGCCTTCC TTTCCAGTTA CTTTGAAATT TGAAATAAAA
 1021 AAAAAAGTTT GCTGTCTTGC TATCAAGTAT AAATAGACCT GCAATATTAA ATCTTTTGTT
 1081 CCTTCGTCAT GTCTTCGATC CCTTTCTTCT TGCTCCTTTT CCTGCACAAT ATTTCAAGCT
1141 TATACCCAAA GGCAATTAAC CAATTCGA
//
Primer: TRP1pro_F RDB13044_A4Ljc.seq
>01951_13044_A4Lj_TRP1pro_F_G09_21.ab1
    1 GTCAGCGATT AGCCACAAGG CAGCTTGGAG TATGTCTGTT ATTAATTTCA CAGGTAGTTC
   61 TGGTCCATTG GTGAAAGTTT GCGGCTTGCA GAGCACAGAG GCCGCAGAAT GTGCTCTAGA
  121 TTCCGATGCT GACTTGCTGG GTATTATATG TGTGCCCAAT AGAAAGAGAA CAATTGACCC
  181 GGTTATTGCA AGGAAAATTT CAAGTCTTGT AAAAGCATAT AAAAATAGTT CAGGCACTCC
  241 GAAATACTTG GTTGGCGTGT TTCGTAATCA ACCTAAGGAG GATGTTTTGG CTCTGGTCAA
  301 TGATTACGGC ATTGATATCG TCCAACTGCA TGGAGATGAG TCGTGGCAAG AATACCAAGA
  361 GTTCCTCGGT TTGCCAGTTA TTAAAAGACT CGTATTTCCA AAAGACTGCA ACATACTACT
  421 CAGTGCAGCT TCACAGAAAC CTCATTCGTT TATTCCCTTG TTTGATTCAG AAGCAGGTGG
  481 GACAGGTGAA CTTTTGGATT GGAACTCGAT TTCTGACTGG GTTGGAAGGC AAGAGAGCCC
  541 CGAAAGCTTA CATTTTATGT TAGCTGGTGG ACTGACGCCA GAAAATGTTG GTGATGCGCT
  601 TAGATTAAAT GGCGTTATTG GTGTTGATGT AAGCGGAGGT GTGGAGACAA ATGGTGTAAA
  661 AGACTCTAAC AAAATAGCAA ATTTCGTCAA AAATGCTAAG AAATAGGTTA TTACTGAGTA
  721 GTATTTATTT AAGTATTGTT TGTGCACTTG CCGGTAGAGG TGTGGTCAAT AAGAGCGACC
  781 TCATGCTATA CCTGAGAAAG CAACCTGACC TACAGGAAAG AGTTACTCAA GAATAAGAAT
  841 TTTCGTTTTA AAACCTAAGA GTCACTTTAA AATTTGTATA CACTTATTTT TTTTTATAAC
  901 TTATTTAATA ATAAAAATCA TAAATCATAA GAAATTCGCC CGGGAATTAG CTTGGCTGCA
  961 CGTCGACTCA CAGATCCTCT TCGGAAATCA GTTTCTGTTC AGAATTTCGA GCTCGGGTAC
 1021 CCGGGGATCC TCTAGAGTCA GCTTTGTAGT TGATTGTATG CTTGGTATAG CTTGAAATAT
 1081 TTGTGCAGAA AAGAACAGCA GGAATGGACG AGACATGACT AGCAACCAAG AATTATAACT
1141 GGCAGTCTAA TTTATACCTT GGAATAAGGC AG
//

Please visit Sequencing and PCR primers for primer information.


References

References

original Iha, H., Shuttle-vector system for Saccharomyces cerevisiae designed to produce C-terminal-Myc-tagged fusion proteins. Biotechniques, 25, 936-938 (1998). PMID 9863043.

2018.12.11

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