RDB01951 - pAMT10

Resource data sheet
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pAMT10

E. coli - S. cerevisiae shuttle vector

Catalog number	RDB01951
Resource name	pAMT10
Alternative name	E. coli-S. cerevisiae shuttle vector
Clone info.	E. coli-S. cerevisiae shuttle vector, 9E10 epitope (Myc-tag), centromere-type ori, TRP1, ADH1 promoter, Ampicillin
Vector backbone	pAMT10 (Plasmid)
Size of vector backbone	4,319 bp
Selectable markers	Amp^r (E. coli), TRP1 (S. cerevisiae)
Depositor	Iha, Hidekatsu \|

Distribution information

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Catalog #	Resource name	Shipping form	Fee (non-profit org.)
RDB01951	pAMT10	DNA solution

Ordering Information [in Japanese] [in English]

References and tips

Electronic file

Electronic file	Sequence RDB01951z.seq from Depositor
Electronic file	Map RDB01951z.gif from Depositor
Electronic file	Map RDB01951.gif from Depositor

Featured content	Expression Vector Backbone of Saccharomyces cerevisiae (English text)

Sequence information

RIKEN BRC has sequenced portions of this material for quality test. Primers and the results are shown below.

Data are summerized on test sheet below.

Test sheet	RDB13044_A4Lj.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: ADHrev	RDB13044_A4Lja.seq
>01951_13044_A4Lj_ADHrev_E09_15.ab1 1 AGAAGCTATC AGATAGATTT TCGTTTTAAA CCTAAGAGTC ACTTTAAAAT TTGTATACAC 61 TTATTTTTTT TATAACTTAT TTAATAATAA AAATCATAAA TCATAAGAAA TTCGCCCGGA 121 ATTAGCTTGG CTGCAGGTCG ACTCACAGAT CCTCTTCGGA AATCAGTTTC TGTTCAGAAT 181 TCGAGCTCGG TACCCGGGGA TCCTCTAGAG TCAGCTTGGA GTTGATTGTA TGCTTGGTAT 241 AGCTTGAAAT ATTGTGCAGA AAAAGAAACA AGGAAGAAAG GGAACGAGAA CAATGACGAG 301 GAAACAAAAG ATTAATAATT GCAGGTCTAT TTATACTTGA TAGCAAGACA GCAAACTTTT 361 TTTTATTTCA AATTCAAGTA ACTGGAAGGA AGGCCGTATA CCGTTGCTCA TTAGAGAGTA 421 GTGTGCGTGA ATGAAGGAAG GAAAAAGTTT CGTGTGCTTC GAGATACCCC TCATCAGCTC 481 TGGAACAACG ACATCTGTTG GTGCTGTCTT TGTCGTTAAT TTTTTCCTTT AGTGTCTTCC 541 ATCATTTTTT TGTCATTGCG GATATGGTGA GACAACAACG GGGGAGAGAG AAAAGAAAAA 601 AAAAGAAAAG AAGTTGCATG CAAGCTCTGC ATGTGTCAGA GGTTTTCACC GTCATCACCG 661 AAACGCGCGA GACGAAAGGG CCTCGTGATA CGCCTATTTT TATAGGTTAA TGTCATGATA 721 ATAATGGTTT CTTAGGGTCC TTTTCATCAC GTGCTATAAA AATAATTATA ATTTAAATTT 781 TTTAATATAA ATATATAAAT TAAAAATAGA AAGTAAAAAA AGAAATTAAA GAAAAAATAG 841 TTTTTGTTTT CCGAAGATGT AAAAGACTCT AGGGGGATCG CCAACAAATA CTACCTTTTA 901 TCTTGCTCTT CCTGCTCTCA GGTATTAATG CCGAATTGTT TCATCTTGTC TGTGTAGAAG 961 ACCACACACG AAAATCCTGT GATTTTACAT TTTACTTTAT CGTTAATCGA ATGTATATCT 1021 ATTTAATCTG CTTTTTCTTG TCTAATAAAA TATATATGTA AGTACGCTTT TTGATGGAAG 1081 TTTTTAAACG TTTGATTATT TTTTTTCTTC ATCGGTAACT TCTTTCTAAC ATTCGTTTAG 1141 TTTAACCTTT CTCTGGA //
Primer: Amp_R	RDB13044_A4Ljb.seq
>01951_13044_A4Lj_Amp_R_F09_18.ab1 1 TTCGTTTTCA TATTATTGAG CATTTATCAG GGTTATTGTC TCATGAGCGG ATACATATTT 61 GAATGTATTT AGAAAAATAA ACAAATAGGG GTTCCGCGCA CATTTCCCCG AAAAGTGCCA 121 CCTGACGGAT CGCTTGCCTG TAACTTACAC GCGCCTCGTA TCTTTTAATG ATGGAATAAT 181 TTGGGAATTT ACTCTGTGTT TATTTATTTT TATGTTTTGT ATTTGGATTT TAGAAAGTAA 241 ATAAAGAAGG TAGAAGAGTT ACGGAATGAA GAAAAAAAAA TAAACAAAGG TTTAAAAAAT 301 TTCAACAAAA AGCGTACTTT ACATATATAT TTATTAGACA AGAAAAGCAG ATTAAATAGA 361 TATACATTCG ATTAACGATA AGTAAAATGT AAAATCACAG GATTTTCGTG TGTGGTCTTC 421 TACACAGACA AGATGAAACA ATTCGGCATT AATACCTGAG AGCAGGAAGA GCAAGATAAA 481 AGGTAGTATT TGTTGGCGAT CCCCCTAGAG TCTTTTACAT CTTCGGAAAA CAAAAACTAT 541 TTTTTCTTTA ATTTCTTTTT TTACTTTCTA TTTTTAATTT ATATATTTAT ATTAAAAAAT 601 TTAAATTATA ATTATTTTTA TAGCACGTGA TGAAAAGGAC CCTAAGAAAC CATTATTATC 661 ATGACATTAA CCTATAAAAA TAGGCGTATC ACGAGGCCCT TTCGTCTCGC GCGTTTCGGT 721 GATGACGGTG AAAACCTCTG ACACATGCAG AGCTTGCATG CAACTTCTTT TCTTTTTTTT 781 TCTTTTCTCT CTCCCCCGTT GTTGTCTCAC CATATCCGCA ATGACAAAAA AATGATGGAA 841 GACACTAAAG GAAAAAATTA ACGACAAAGA CAGCACCAAC AGATGTCGTT GTTCCAGAGC 901 TGATGAGGGG TATCTCGAAG CACACGAAAC TTTTTCCTTC CTTCATTCAC GCACACTACT 961 CTCTAATGAG CAACGGTATA CGGGCCTTCC TTTCCAGTTA CTTTGAAATT TGAAATAAAA 1021 AAAAAAGTTT GCTGTCTTGC TATCAAGTAT AAATAGACCT GCAATATTAA ATCTTTTGTT 1081 CCTTCGTCAT GTCTTCGATC CCTTTCTTCT TGCTCCTTTT CCTGCACAAT ATTTCAAGCT 1141 TATACCCAAA GGCAATTAAC CAATTCGA //
Primer: TRP1pro_F	RDB13044_A4Ljc.seq
>01951_13044_A4Lj_TRP1pro_F_G09_21.ab1 1 GTCAGCGATT AGCCACAAGG CAGCTTGGAG TATGTCTGTT ATTAATTTCA CAGGTAGTTC 61 TGGTCCATTG GTGAAAGTTT GCGGCTTGCA GAGCACAGAG GCCGCAGAAT GTGCTCTAGA 121 TTCCGATGCT GACTTGCTGG GTATTATATG TGTGCCCAAT AGAAAGAGAA CAATTGACCC 181 GGTTATTGCA AGGAAAATTT CAAGTCTTGT AAAAGCATAT AAAAATAGTT CAGGCACTCC 241 GAAATACTTG GTTGGCGTGT TTCGTAATCA ACCTAAGGAG GATGTTTTGG CTCTGGTCAA 301 TGATTACGGC ATTGATATCG TCCAACTGCA TGGAGATGAG TCGTGGCAAG AATACCAAGA 361 GTTCCTCGGT TTGCCAGTTA TTAAAAGACT CGTATTTCCA AAAGACTGCA ACATACTACT 421 CAGTGCAGCT TCACAGAAAC CTCATTCGTT TATTCCCTTG TTTGATTCAG AAGCAGGTGG 481 GACAGGTGAA CTTTTGGATT GGAACTCGAT TTCTGACTGG GTTGGAAGGC AAGAGAGCCC 541 CGAAAGCTTA CATTTTATGT TAGCTGGTGG ACTGACGCCA GAAAATGTTG GTGATGCGCT 601 TAGATTAAAT GGCGTTATTG GTGTTGATGT AAGCGGAGGT GTGGAGACAA ATGGTGTAAA 661 AGACTCTAAC AAAATAGCAA ATTTCGTCAA AAATGCTAAG AAATAGGTTA TTACTGAGTA 721 GTATTTATTT AAGTATTGTT TGTGCACTTG CCGGTAGAGG TGTGGTCAAT AAGAGCGACC 781 TCATGCTATA CCTGAGAAAG CAACCTGACC TACAGGAAAG AGTTACTCAA GAATAAGAAT 841 TTTCGTTTTA AAACCTAAGA GTCACTTTAA AATTTGTATA CACTTATTTT TTTTTATAAC 901 TTATTTAATA ATAAAAATCA TAAATCATAA GAAATTCGCC CGGGAATTAG CTTGGCTGCA 961 CGTCGACTCA CAGATCCTCT TCGGAAATCA GTTTCTGTTC AGAATTTCGA GCTCGGGTAC 1021 CCGGGGATCC TCTAGAGTCA GCTTTGTAGT TGATTGTATG CTTGGTATAG CTTGAAATAT 1081 TTGTGCAGAA AAGAACAGCA GGAATGGACG AGACATGACT AGCAACCAAG AATTATAACT 1141 GGCAGTCTAA TTTATACCTT GGAATAAGGC AG //

Please visit Sequencing and PCR primers for primer information.

References

original	Iha, H., Shuttle-vector system for Saccharomyces cerevisiae designed to produce C-terminal-Myc-tagged fusion proteins. Biotechniques, 25, 936-938 (1998). PMID 9863043.

2018.12.11

GNP_filter3_RDBDEP_html_181012.pl