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pAML20

E. coli - S. cerevisiae shuttle vector

Catalog number RDB01950
Resource name pAML20
Alternative name E. coli-S. cerevisiae shuttle vector
Clone info. E. coli-S. cerevisiae shuttle vector, 9E10 epitope (Myc-tag), 2micron ori, LEU2, ADH1 promoter, Ampicillin
Vector backbone pAML20 (Plasmid)
Size of vector backbone 5,916 bp
Selectable markers Amp^r (E. coli), LEU2 (S. cerevisiae)
Depositor|Developer Iha, Hidekatsu |
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External Database

            Reference sequence
              

            Distribution information

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            RDB01950 pAML20 DNA solution

            Ordering Information [in Japanese] [in English]
            Please review the QC test results indicated by check icon below as well as clone information before placing your order.

            References and tips

            Electronic file

            Sequence RDB01950z.seq
            Remarks, protocol and/or map (gif) RDB01950.gif
            Remarks, protocol and/or map (gif) RDB01950z.gif

            Original reference

            original Iha, H., Shuttle-vector system for Saccharomyces cerevisiae designed to produce C-terminal-Myc-tagged fusion proteins. Biotechniques, 25, 936-938 (1998). PMID 9863043.

            Further references such as user reports and related articles (go to bottom)

            Featured content

            Featured content Expression Vector Backbone of Saccharomyces cerevisiae (English text)

            Sequence information

            RIKEN BRC has sequenced portions of this material for quality test.
            Please review the QC test results indicated by check icon as well as clone information before placing your order.

            Test sheet RDB12313_A5Bg.pdf check

            Nucleotide sequence of a portion of this resource (if available).

            Primer: ADH_rev
            Sequence file: RDB12313_A5Bga.seq check
            >01950_12313_A5Bg_ADHrev_A07_01.ab1
                1 TGGACTCTCA AGATAAGAAT TTTCGTTTTA AACCTAAGAG TCACTCCTAA AATTTGTATA
               61 CACTTATTTT TTTTATAACT TATTTAATAA TAAAAATCAT AAATCATAAG AAATTCGCCC
              121 GGAATTAGCT TGGCTGCAGG TCGACTCACA GATCCTCTTC GGAAATCAGT TTCTGTTCAG
              181 AATTCGAGCT CGGTACCCGG GGATCCTCTA GAGTCAGCTT GGAGTTGATT GTATGCTTGG
              241 TATAGCTTGA AATATTGTGC AGAAAAAGAA ACAAGGAAGA AAGGGAACGA GAACAATGAC
              301 GAGGAAACAA AAGATTAATA ATTGCAGGTC TATTTATACT TGATAGCAAG ACAGCAAACT
              361 TTTTTTTATT TCAAATTCAA GTAACTGGAA GGAAGGCCGT ATACCGTTGC TCATTAGAGA
              421 GTAGTGTGCG TGAATGAAGG AAGGAAAAAG TTTCGTGTGC TTCGAGATAC CCCTCATCAG
              481 CTCTGGAACA ACGACATCTG TTGGTGCTGT CTTTGTCGTT AATTTTTTCC TTTAGTGTCT
              541 TCCATCATTT TTTTGTCATT GCGGATATGG TGAGACAACA ACGGGGGAGA GAGAAAAGAA
              601 AAAAAAAGAA AAGAAGTTGC ATGCAAGCAA CGAAGCATCT GTGCTTCATT TTGTAGAACA
              661 AAAATGCAAC GCGAGAGCGC TAATTTTTCA AACAAAGAAT CTGAGCTGCA TTTTTACAGA
              721 ACAGAAATGC AACGCGAAAG CGCTATTTTA CCAACGAAGA ATCTGTGCTT CATTTTTGTA
              781 AAACAAAAAT GCAACGCGAG AGCGCTAATT TTTCAAACAA AGAATCTGAG CTGCATTTTT
              841 ACAGAACAGA AATGCAACGC GAGAGCGCTA TTTTACCAAC AAAGAATCTA TACTTCTTTT
              901 TTTGTTCTAC AAAAAATGCA TCCCGAGAGC GCTATTTTTT CTAACAAAGC ATCTTAGATT
              961 ACTTTTTTTT TCTCCCTTTG TGCGCTCTAT AATGCAGTCT CTTTGATAAA CTTTTTTGCA
             1021 CTGTAGGTCC GTTTAAAGGT TAGAAGAACG GCTACTTTGG GTGGTCTTAT TTTTCTCTTT
             1081 CCATAAAAAA AGGTCTGGAC TCGACTTCCC CGCGATTTTA CTGGAAGTTA ACCTTAAGCC
            1141 GGGA
            //
            Primer: Amp_R
            Sequence file: RDB12313_A5Bgb.seq check
            >01950_12313_A5Bg_Amp_R_B07_04.ab1
                1 TTTTTTTATA TTATTGAGCA TTTATCAGGG TTATTGTCTC ATGAGCGGAT ACATATTTGA
               61 ATGTATTTAG AAAAATAAAC AAATAGGGGT TCCGCGCACA TTTCCCCGAA AAGTGCCACC
              121 TGACGTCTAA GAAACCATTA TTATCATGAC ATTAACCTAT AAAAATAGGC GTATCACGAG
              181 GCCCTTTCGT CTCGCGCGTT TCGGTGATGA CGGTGAAAAC CTCTGACACA TGCAGCTCCC
              241 GGAGACGGTC ACAGCTTGTC TGTAAGCGGA TGCCGGGAGC AGACAAGCCC GTCAGGGCGC
              301 GTCAGCGGGT GTTGGCGGGT GTCGGGGCTG GCTTAACTAT GCGGCATCAG AGCAGATTGT
              361 ACTGAGAGTG CACCATAACG CATTTAAGCA TAAACACGCA CTATGCCGTT CTTCTCATGT
              421 ATATATATAT ACAGGCAACA CGCAGATATA GGTGCGACGT GAACAGTGAG CTGTATGTGC
              481 GCAGCTCGCG TTGCATTTTC GGAAGCGCTC GTTTTCGGAA ACGCTTTGAA GTTCCTATTC
              541 CGAAGTTCCT ATTCTCTAGC TAGAAAGTAT AGGAACTTCA GAGCGCTTTT GAAAACCAAA
              601 AGCGCTCTGA AGACGCACTT TCAAAAAACC AAAAACGCAC CGGACTGTAA CGAGCTACTA
              661 AAATATTGCG AATACCGCTT CCACAAACAT TGCTCAAAAG TATCTCTTTG CTATATATCT
              721 CTGTGCTATA TCCCTATATA ACCTACCCAT CCACCTTTCG CTCCTTGAAC TTGCATCTAA
              781 ACTCGACCTC TACATTTTTT ATGTTTATCT CTAGTATTAC TCTTTAGACA AAAAAATTGT
              841 AGTAAGAACT ATTCATAGAG TGAATCGAAA ACAATACGAA AATGTAAACA TTTCCTATAC
              901 GTAGTATATA GAGACAAAAT AGAAGAAACC GTTCATAATT TTCTGACCAA TGAAGAATCA
              961 TCAACGCTAT CACTTTCTGT TCACAAAGTA TGCGCAATCC ACATCGGTAT AGAATATAAT
             1021 CGGGGATGCC CTTTTATCTT GAAAAAATGC ACCCGCAGCT TTCGCTAGTA TCAGTAAACG
             1081 CGGGAGTTGA GGTCAGCTTT TTTATGTAAG AGAAATAGAC CTAAGCTAGC ATTCGTCCGT
            1141 AACCCGTA
            //
            Primer: LEU2pro_F
            Sequence file: RDB12313_A5Bgc.seq check
            >01950_12313_A5Bg_LEU2pro_F_C07_07.ab1
                1 ACCTTTCACT GCATATTATT TAAGGACCTA TTGTTTTTTC CAATAGGTGG TTAGCAATCG
               61 TCTTACTTTC TAACTTTTCT TACCTTTTAC ATTTCAGCAA TATATATATA TATATTTCAA
              121 GGATATACCA TTCTAATGTC TGCCCCTAAG AAGATCGTCG TTTTGCCAGG TGACCACGTT
              181 GGTCAAGAAA TCACAGCCGA AGCCATTAAG GTTCTTAAAG CTATTTCTGA TGTTCGTTCC
              241 AATGTCAAGT TCGATTTCGA AAATCATTTA ATTGGTGGTG CTGCTATCGA TGCTACAGGT
              301 GTTCCACTTC CAGATGAGGC GCTGGAAGCC TCCAAGAAGG CTGATGCCGT TTTGTTAGGT
              361 GCTGTGGGTG GTCCTAAATG GGGTACAGGT AGTGTTAGAC CTGAACAAGG TTTACTAAAA
              421 ATCCGTAAAG AACTTCAATT GTACGCCAAC TTAAGACCAT GTAACTTTGC ATCCGACTCT
              481 CTTTTAGACT TATCTCCAAT CAAGCCACAA TTTGCTAAAG GTACTGACTT CGTTGTTGTC
              541 AGAGAATTAG TGGGAGGTAT TTACTTTGGT AAGAGAAAGG AAGACGATGG TGATGGTGTC
              601 GCTTGGGATA GTGAACAATA CACCGTTCCA GAAGTGCAAA GAATCACAAG AATGGCCGCT
              661 TTCATGGCCC TACAACATGA GCCACCATTG CCTATTTGGT CCTTGGATAA AGCTAATGTT
              721 TTGGCCTCTT CAAGATTATG GAGAAAAACT GTGGAGGAAA CCATCAAGAA CGAATTTCCT
              781 ACATTGAAGG TTCAACATCA ATTGATTGAT TCTGCCGCCA TGATCCTAGT TAAGAACCCA
              841 ACCCACCTAA ATGGTATTAT AATCACCAGC AACATGTTTG GTGATATCAT CTCCGATGAA
              901 GCCTCCGTTA TCCCAGGTTC CTTGGGTTTG TTGCCATCTG CGTCCTTGGG CCTCTTTGCC
              961 AGACAAGAAC ACCGCATTTG GGTTTGTACG AACCCATGCC CACGGGTTCT GCTCCAGATT
             1021 TGCCCAAAAG ATAACGTCAC CCCTATCGCC ACTATCTGTC TGCTGCATTG AATGGTGAAA
             1081 TGTTCATTGA ACTGCTGAGA AGGTAGTCCA TGGAGGATGC CAGCTTAAAA AGGTTTTGCC
            1141 T
            //

            Please visit Sequencing and PCR primers for primer information.


            References

            Original, user report and related articles

            original Iha, H., Shuttle-vector system for Saccharomyces cerevisiae designed to produce C-terminal-Myc-tagged fusion proteins. Biotechniques, 25, 936-938 (1998). PMID 9863043.

            2022.02.09

            GNP_filter3_RDBDEP_html_220112.pl