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pAML20

E. coli - S. cerevisiae shuttle vector

Catalog number RDB01950
Resource name pAML20
Alternative name E. coli-S. cerevisiae shuttle vector
Clone info. E. coli-S. cerevisiae shuttle vector, 9E10 epitope (Myc-tag), 2micron ori, LEU2, ADH1 promoter, Ampicillin
Vector backbone pAML20 (Plasmid)
Size of vector backbone 5,916 bp
Selectable markers Amp^r (E. coli), LEU2 (S. cerevisiae)
Depositor Iha, Hidekatsu |

Distribution information

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RDB01950 pAML20 DNA solution

Ordering Information [in Japanese] [in English]

References and tips

Electronic file

Electronic file Sequence RDB01950z.seq from Depositor
Electronic file Map RDB01950z.gif from Depositor
Electronic file Map RDB01950.gif from Depositor

Featured content

Featured content Expression Vector Backbone of Saccharomyces cerevisiae (English text)

References

original Iha, H., Shuttle-vector system for Saccharomyces cerevisiae designed to produce C-terminal-Myc-tagged fusion proteins. Biotechniques, 25, 936-938 (1998). PMID 9863043.

User reports and related articles (go to bottom)


Sequence information

RIKEN BRC has sequenced portions of this material for quality test. Primers and the results are shown below.

Data are summerized on test sheet below.

Test sheet RDB12313_A5Bg.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: ADH_rev RDB12313_A5Bga.seq
>01950_12313_A5Bg_ADHrev_A07_01.ab1
    1 TGGACTCTCA AGATAAGAAT TTTCGTTTTA AACCTAAGAG TCACTCCTAA AATTTGTATA
   61 CACTTATTTT TTTTATAACT TATTTAATAA TAAAAATCAT AAATCATAAG AAATTCGCCC
  121 GGAATTAGCT TGGCTGCAGG TCGACTCACA GATCCTCTTC GGAAATCAGT TTCTGTTCAG
  181 AATTCGAGCT CGGTACCCGG GGATCCTCTA GAGTCAGCTT GGAGTTGATT GTATGCTTGG
  241 TATAGCTTGA AATATTGTGC AGAAAAAGAA ACAAGGAAGA AAGGGAACGA GAACAATGAC
  301 GAGGAAACAA AAGATTAATA ATTGCAGGTC TATTTATACT TGATAGCAAG ACAGCAAACT
  361 TTTTTTTATT TCAAATTCAA GTAACTGGAA GGAAGGCCGT ATACCGTTGC TCATTAGAGA
  421 GTAGTGTGCG TGAATGAAGG AAGGAAAAAG TTTCGTGTGC TTCGAGATAC CCCTCATCAG
  481 CTCTGGAACA ACGACATCTG TTGGTGCTGT CTTTGTCGTT AATTTTTTCC TTTAGTGTCT
  541 TCCATCATTT TTTTGTCATT GCGGATATGG TGAGACAACA ACGGGGGAGA GAGAAAAGAA
  601 AAAAAAAGAA AAGAAGTTGC ATGCAAGCAA CGAAGCATCT GTGCTTCATT TTGTAGAACA
  661 AAAATGCAAC GCGAGAGCGC TAATTTTTCA AACAAAGAAT CTGAGCTGCA TTTTTACAGA
  721 ACAGAAATGC AACGCGAAAG CGCTATTTTA CCAACGAAGA ATCTGTGCTT CATTTTTGTA
  781 AAACAAAAAT GCAACGCGAG AGCGCTAATT TTTCAAACAA AGAATCTGAG CTGCATTTTT
  841 ACAGAACAGA AATGCAACGC GAGAGCGCTA TTTTACCAAC AAAGAATCTA TACTTCTTTT
  901 TTTGTTCTAC AAAAAATGCA TCCCGAGAGC GCTATTTTTT CTAACAAAGC ATCTTAGATT
  961 ACTTTTTTTT TCTCCCTTTG TGCGCTCTAT AATGCAGTCT CTTTGATAAA CTTTTTTGCA
 1021 CTGTAGGTCC GTTTAAAGGT TAGAAGAACG GCTACTTTGG GTGGTCTTAT TTTTCTCTTT
 1081 CCATAAAAAA AGGTCTGGAC TCGACTTCCC CGCGATTTTA CTGGAAGTTA ACCTTAAGCC
1141 GGGA
//
Primer: Amp_R RDB12313_A5Bgb.seq
>01950_12313_A5Bg_Amp_R_B07_04.ab1
    1 TTTTTTTATA TTATTGAGCA TTTATCAGGG TTATTGTCTC ATGAGCGGAT ACATATTTGA
   61 ATGTATTTAG AAAAATAAAC AAATAGGGGT TCCGCGCACA TTTCCCCGAA AAGTGCCACC
  121 TGACGTCTAA GAAACCATTA TTATCATGAC ATTAACCTAT AAAAATAGGC GTATCACGAG
  181 GCCCTTTCGT CTCGCGCGTT TCGGTGATGA CGGTGAAAAC CTCTGACACA TGCAGCTCCC
  241 GGAGACGGTC ACAGCTTGTC TGTAAGCGGA TGCCGGGAGC AGACAAGCCC GTCAGGGCGC
  301 GTCAGCGGGT GTTGGCGGGT GTCGGGGCTG GCTTAACTAT GCGGCATCAG AGCAGATTGT
  361 ACTGAGAGTG CACCATAACG CATTTAAGCA TAAACACGCA CTATGCCGTT CTTCTCATGT
  421 ATATATATAT ACAGGCAACA CGCAGATATA GGTGCGACGT GAACAGTGAG CTGTATGTGC
  481 GCAGCTCGCG TTGCATTTTC GGAAGCGCTC GTTTTCGGAA ACGCTTTGAA GTTCCTATTC
  541 CGAAGTTCCT ATTCTCTAGC TAGAAAGTAT AGGAACTTCA GAGCGCTTTT GAAAACCAAA
  601 AGCGCTCTGA AGACGCACTT TCAAAAAACC AAAAACGCAC CGGACTGTAA CGAGCTACTA
  661 AAATATTGCG AATACCGCTT CCACAAACAT TGCTCAAAAG TATCTCTTTG CTATATATCT
  721 CTGTGCTATA TCCCTATATA ACCTACCCAT CCACCTTTCG CTCCTTGAAC TTGCATCTAA
  781 ACTCGACCTC TACATTTTTT ATGTTTATCT CTAGTATTAC TCTTTAGACA AAAAAATTGT
  841 AGTAAGAACT ATTCATAGAG TGAATCGAAA ACAATACGAA AATGTAAACA TTTCCTATAC
  901 GTAGTATATA GAGACAAAAT AGAAGAAACC GTTCATAATT TTCTGACCAA TGAAGAATCA
  961 TCAACGCTAT CACTTTCTGT TCACAAAGTA TGCGCAATCC ACATCGGTAT AGAATATAAT
 1021 CGGGGATGCC CTTTTATCTT GAAAAAATGC ACCCGCAGCT TTCGCTAGTA TCAGTAAACG
 1081 CGGGAGTTGA GGTCAGCTTT TTTATGTAAG AGAAATAGAC CTAAGCTAGC ATTCGTCCGT
1141 AACCCGTA
//
Primer: LEU2pro_F RDB12313_A5Bgc.seq
>01950_12313_A5Bg_LEU2pro_F_C07_07.ab1
    1 ACCTTTCACT GCATATTATT TAAGGACCTA TTGTTTTTTC CAATAGGTGG TTAGCAATCG
   61 TCTTACTTTC TAACTTTTCT TACCTTTTAC ATTTCAGCAA TATATATATA TATATTTCAA
  121 GGATATACCA TTCTAATGTC TGCCCCTAAG AAGATCGTCG TTTTGCCAGG TGACCACGTT
  181 GGTCAAGAAA TCACAGCCGA AGCCATTAAG GTTCTTAAAG CTATTTCTGA TGTTCGTTCC
  241 AATGTCAAGT TCGATTTCGA AAATCATTTA ATTGGTGGTG CTGCTATCGA TGCTACAGGT
  301 GTTCCACTTC CAGATGAGGC GCTGGAAGCC TCCAAGAAGG CTGATGCCGT TTTGTTAGGT
  361 GCTGTGGGTG GTCCTAAATG GGGTACAGGT AGTGTTAGAC CTGAACAAGG TTTACTAAAA
  421 ATCCGTAAAG AACTTCAATT GTACGCCAAC TTAAGACCAT GTAACTTTGC ATCCGACTCT
  481 CTTTTAGACT TATCTCCAAT CAAGCCACAA TTTGCTAAAG GTACTGACTT CGTTGTTGTC
  541 AGAGAATTAG TGGGAGGTAT TTACTTTGGT AAGAGAAAGG AAGACGATGG TGATGGTGTC
  601 GCTTGGGATA GTGAACAATA CACCGTTCCA GAAGTGCAAA GAATCACAAG AATGGCCGCT
  661 TTCATGGCCC TACAACATGA GCCACCATTG CCTATTTGGT CCTTGGATAA AGCTAATGTT
  721 TTGGCCTCTT CAAGATTATG GAGAAAAACT GTGGAGGAAA CCATCAAGAA CGAATTTCCT
  781 ACATTGAAGG TTCAACATCA ATTGATTGAT TCTGCCGCCA TGATCCTAGT TAAGAACCCA
  841 ACCCACCTAA ATGGTATTAT AATCACCAGC AACATGTTTG GTGATATCAT CTCCGATGAA
  901 GCCTCCGTTA TCCCAGGTTC CTTGGGTTTG TTGCCATCTG CGTCCTTGGG CCTCTTTGCC
  961 AGACAAGAAC ACCGCATTTG GGTTTGTACG AACCCATGCC CACGGGTTCT GCTCCAGATT
 1021 TGCCCAAAAG ATAACGTCAC CCCTATCGCC ACTATCTGTC TGCTGCATTG AATGGTGAAA
 1081 TGTTCATTGA ACTGCTGAGA AGGTAGTCCA TGGAGGATGC CAGCTTAAAA AGGTTTTGCC
1141 T
//

Please visit Sequencing and PCR primers for primer information.


References

References

original Iha, H., Shuttle-vector system for Saccharomyces cerevisiae designed to produce C-terminal-Myc-tagged fusion proteins. Biotechniques, 25, 936-938 (1998). PMID 9863043.

2018.12.11

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